IndyWatch Health Watch Feed Archiver

Go Back:30 Days | 7 Days | 2 Days | 1 Day

IndyWatch Health Watch Feed Today.

Go Forward:1 Day | 2 Days | 7 Days | 30 Days

IndyWatch Health Watch Feed was generated at Community Resources IndyWatch.

Sunday, 17 June

17:57

5G Mini Cell TowersJunk Yards On A PoleWill Affect Your Lifestyle More Than You Know "IndyWatch Feed Health"

5G Mini Cell TowersJunk Yards On A PoleWill Affect Your Lifestyle More Than You KnowBy Catherine J. Frompovich

How would you like this mini cell tower parked outside your front door or your bedroom window or on a pole in your backyard? And you cannot have it removed, thanks to the U.S. Federal Communications Commission!

5G Mini Cell TowersJunk Yards On A PoleWill Affect Your Lifestyle More Than You KnowSource: EMF Safety Network

That little monster will pump out 5G wireless networks at speeds consumers literally may die for, as the amount of microwaves 5G will be p...

17:28

Is Psychosis Natural? "IndyWatch Feed Health"

The physiological process of psychosis is ecologically purposeful. Not good nor bad, but part of what Nature does trying to grow.

17:02

Difficult Fathers Day for Dads Who Lost Their Children to Corrupt Child Protection System "IndyWatch Feed Health"

Fathers collage

Some of the fathers who lost their children wrongly to Child Protection Services that Health Impact News has covered.

by Terri LaPoint
Health Impact News

It is Fathers Day. The day that we honor dads. The holiday began decades ago as a day to celebrate the strong, heroic, protective men who inspire, encourage, and push us to be the best we can be.

Most of us have a beautiful, nostalgic image of what today is supposed to be like, but for too many of our readers, that image is a painful reminder of what could be, what should be but isnt.

Children and parents are being separated by the hundreds of thousands in the United States. There are many fathers who want nothing more than to be with their children today, but a judge or a social worker has decided that they cant be.

If they had done something wrong, the bitter separation might be somewhat easier to bear, but many dads today are in a situation that was created by a false allegation or by asking for a second medical opinion. They wanted to protect their children and give their best, but people who see their children as little more than dollar signs have stolen that basic human right from them.

We say in our culture that we value fathers, but attitudes in our courts, media, and entertainment reflect otherwise.

Fatherhood is the noblest calling that a man can have. It is the opportunity to impact and shape the lives of those who follow in his footsteps, as he provides for, protects, and comforts his children.

To a young child, a dad is bigger than life, even better than Superman. To the child who is now an adult, father is still the person who we turn to when life doesnt make sense or we need strong arms to guide or comfort us.

The presence of a childs father in ones life impacts that child in countless significant ways, and the impact is lifelong. The absence of a father also makes a profound impact in a childs life.

The very concept of fatherhood, once celebrated and embraced on all levels of society, has been shattered and the role of father...

16:50

Difficult Fathers Day for Dads Who Lost Their Children to Corrupt Child Protection System "IndyWatch Feed Health"

Fathers collage

Some of the fathers who lost their children wrongly to Child Protection Services that Health Impact News has covered.

by Terri LaPoint
Health Impact News

It is Fathers Day. The day that we honor dads. The holiday began decades ago as a day to celebrate the strong, heroic, protective men who inspire, encourage, and push us to be the best we can be.

Most of us have a beautiful, nostalgic image of what today is supposed to be like, but for too many of our readers, that image is a painful reminder of what could be, what should be but isnt.

Children and parents are being separated by the hundreds of thousands in the United States. There are many fathers who want nothing more than to be with their children today, but a judge or a social worker has decided that they cant be.

If they had done something wrong, the bitter separation might be somewhat easier to bear, but many dads today are in a situation that was created by a false allegation or by asking for a second medical opinion. They wanted to protect their children and give their best, but people who see their children as little more than dollar signs have stolen that basic human right from them.

We say in our culture that we value fathers, but attitudes in our courts, media, and entertainment reflect otherwise.

Fatherhood is the noblest calling that a man can have. It is the opportunity to impact and shape the lives of those who follow in his footsteps, as he provides for, protects, and comforts his children.

To a young child, a dad is bigger than life, even better than Superman. To the child who is now an adult, father is still the person who we turn to when life doesnt make sense or we need strong arms to guide or comfort us.

The presence of a childs father in ones life impacts that child in countless significant ways, and the impact is lifelong. The absence of a father also makes a profound impact in a childs life.

The very concept of fatherhood, once celebrated and embraced on all levels of society, has been shattered and the role of father has...

16:43

Nine Herbs to Detox the Liver and Protect the Body From Toxins and Pollutants "IndyWatch Feed Health"

Source: Nine Herbs to Detox the Liver and Protect the Body From Toxins and Pollutants For more content like this visit REALfarmacy.com.

Imagine a fish tank with a motorized water filter. If the filter is clogged with toxins from the tank, the entire tank becomes filled with the same contaminants. In our system, the filter is the liver, the tank is the body, and the fish that has to live in it is you. Thankfully, a number []

Source: Nine Herbs to Detox the Liver and Protect the Body From Toxins and Pollutants Learn more at REALfarmacy.com - Healthy News and Information.

15:55

As I Lay Dying.. LA Times Writers Last Words Will Make You Question Entire Breast Cancer Industry "IndyWatch Feed Health"

wide_3128

Laurie Becklund, who passed away from breast cancer this year. PHOTO: Medicinex.Stanford.edu

 

 

 

15:52

Meet the scientific sleuths: Eleven whove had an impact on the scientific literature "IndyWatch Feed Health"

Over the years, we have written about a number of the sleuths who, on their own time and often at great risks to their careers or finances, have looked for issues in the scientific literature. Heres a sampling: Elisabeth Eagle Eyes Bik showed that one in 25 papers she examined had evidence of inappropriate image manipulation. Continue reading Meet the scientific sleuths: Eleven whove had an impact on the scientific literature

15:21

How Turmeric Can Save the Aging Brain From Dementia and Premature Death "IndyWatch Feed Health"

How Turmeric Can Save the Aging Brain From Dementia and Premature Death

Presently, there are no pharmaceutical interventions that effectively slow, and certainly not reverse, age-related cerebrovascular pathologies linked to conditions such as Alzheimer's disease and stroke. A new study on turmeric extract, however, indicates that a natural curative agent already exists and is close to the everyday consumer as their spice rack. 

read more

13:16

Why Fathers Day is Mother's Day for This Autism Dad "IndyWatch Feed Health"

By Chuck Hancock Five years ago on Father's Day, my mother died. Mary Hancock had broken her arm several weeks before her death, and her health just started to go downhill. The medical professionals had trouble managing her pain. I...

12:01

Mentally Ill Students Say Universities are Leaving Them Behind "IndyWatch Feed Health"

When Stanford student Emily W. attempted suicide in 2013, a representative of the elite California university told her she was a liability, and people like you tend not to succeed, or words to that effect before revoking her housing and forcing her to take a leave of absence, according to a complaint filed last month in the U.S. District Court for the Northern District of California.

The suit, filed by the Berkeley-based Disability Rights Advocates (DRA), seeks class-action status for students like Emily affected by what DRA calls discriminatory policies and practices that have made it hard for them to thrive at one of the most prestigious universities in the United States. It could have big implications for the way universities handle mental health crises and students in need of support in the future.

Article 

02:29

Dads Who Go Beyond "IndyWatch Feed Health"

By Cathy Jameson I saw one of the sweetest videos a little over a week ago. You may have seen it, too. A dad jumped on stage to help his young ballerina daughter. Plagued with stage fright, she was in...

01:03

Special Forces Father "IndyWatch Feed Health"

By Dara Berger My husband is no where near perfect, but one thing I can say is he is an amazing father and sometimes it actually takes my breath away. On most days I find myself barking at my husband...

Go Back:30 Days | 7 Days | 2 Days | 1 Day

IndyWatch Health Watch Feed Today.

Go Forward:1 Day | 2 Days | 7 Days | 30 Days

Saturday, 16 June

20:51

Geoengineering Watch Global Alert News, June 16, 2018, #149 "IndyWatch Feed Health"

wwr3Dane Wigington GeoengineeringWatch.org So many breaking dire headlines about the rapidly deteriorating state of the environment are pouring in that it is impossible to cover and comprehend all the incoming reports, but we must try just the same. The June 16th installment of Global Alert News is below. Denial wont save us, apathy wont save us, inaction wont save

17:54

Republican State Convention in San Antonio supports banning fluoridation in Texas "IndyWatch Feed Health"

The following was passed at the Republican State Convention in San Antonio that ended today:

245. Fluoride in Water Supply: The Republican Party of Texas supports banning the fluoridation of the Texas water supply.

See http://fluoridealert.org/wp-content/uploads/tx-gop.platform.june_.2018.pdf

17:00

Deadly Brain-Eating Amoeba Found in Louisiana Water Supply for the Third Time "IndyWatch Feed Health"

By Lily Dane

Residents of Terrebonne Parish in Louisiana are being warned once again: Independent testing of the water supply returned positive results for Naegleria fowleri also known as the brain-eating amoeba   which causes fatal brain swelling and tissue destruction.

The Terrebonne Consolidated Waterworks District issued the notification Sunday and has temporarily switched from using chloramine to free chlorine, which should kill off the amoebas, reports WWLTV.com.

Officials say they are doing what they can, but residents are understandably afraid:

Residents, once again, are ignoring their swimming pools and the nearby bayous, terrified of something they cant see but officials warn is there. This is the third time since 2015 the deadly amoeba has been detected in the water, impacting all fresh water sources in the parish. That includes drinking water, the water in the bayous and pools and the water used for showers and baths.

It kinda freaks me out because this is my home, I cant do what I usually do, Lindsey Dupre said. I want to know Im secure rather than freak out over an amoeba.

Norma...

16:19

For Almost Neurotypical Kids Who Struggle "IndyWatch Feed Health"

June 16, 2018 Ive written many times about my older son, who was diagnosed with an autism spectrum disorder in the fall of 2000. What I havent shared publicly, is the story of the little baby who was in the Continue reading

The post For Almost Neurotypical Kids Who Struggle appeared first on The Thinking Moms' Revolution.

15:30

Weekend reads: How to kill zombie citations; wanted: 6,000 new journals; does peer review matter anymore? "IndyWatch Feed Health"

Before we present this weeks Weekend Reads, a question: Do you enjoy our weekly roundup? If so, we could really use your help. Would you consider a tax-deductible donation to support Weekend Reads, and our daily work? Thanks in advance. The week at Retraction Watch featured a retraction and replacement of a diet study in the Continue reading Weekend reads: How to kill zombie citations; wanted: 6,000 new journals; does peer review matter anymore?

15:09

Learning to Speak Psychotic "IndyWatch Feed Health"

One of the biggest barriers that people who are psychotic face is one of communication: other people often have trouble understanding what theyre talking about. The way that so-called psychotics describe their experience (their actual language) and their ideas are simply foreign to most people, and this lack of clear communication is what gets psychotics labelled as psychotic in the first place, and thus it leads to a breakdown between the psychotic and the rest of society. This is a loss to both groups.

The way to address this might be if everyone, or at least those who are in the helping professions, learned to Speak Psychotic. I am referring to Psychotic as a kind of language, then almost a kind of code that is based on a metaphorical (or possibly even factual) system which, I hope you will see, is focused on certain relatively limited groups of themes once you learn to interpret them for what they mean.

1. The Nature of the Code

To begin with a story:

A little more than three years ago, in 2015, I was confined for about four months in New Hampshire Hospital, my states psychiatric facility (which I regard frankly as a prison). I was going through a psychotic period myself, characterized by all sorts of what they call delusions, a term which is entirely a misnomer we will let that pass for the moment as well as by hearing voices all day long, every single day.

Shortly after I first arrived I made the acquaintance of a woman who had long, dyed-black hair and all kinds of old scars all over her face (possibly burn or acne scars), and she wore black shirts and black pants and black boots and all sorts of little trinkets like bracelets and necklaces from the hospital/prisons gift shop. She would stalk up and down the halls of the unit, and each time she passed me she would look at me in this blank way out of the blank mask that her face had become with an expression that said she was studying me, looking out of her prison of psychological Hell with a desperate expression in her eyes, and after I nodded at her in acknowledgment, she began to talk to me.

She spoke in what might have seemed like a kind of gibberish, a kind of word salad that was near impenetrable, but I kept listening to her and gradually, over the course of a couple of weeks, I began to see the outlines of what she was talking about.

She talked all the time about internet porn and how there were people out there who had robbed her, and I realized that she was probably the victim of sexual abuse and financial exploit...

15:03

The Only Dandruff Cure Youll Ever Need "IndyWatch Feed Health"

Have you ever looked down at your clothes and noticed the embarrassing, tell-tale signs of a flaking scalp? If so, youre not alonedandruff affects one in five people in the United States. But its important to understand the reason for those flakes: It could be a sign of normal dryness, a pesky fungal infection, or a serious autoimmune condition

read more

14:21

Health Officials Alarmed: Texas Vaccine Resistance Up 20-Times Over Past Decade "IndyWatch Feed Health"

Texas has always been known for its libertarian and conservative foundation. It seems with vaccines, the trend when compared nationally, remains exactly in line with such beliefs. A new study has four major cities in...

The post Health Officials Alarmed: Texas Vaccine Resistance Up 20-Times Over Past Decade appeared first on Vaxxter.

12:01

The Lifespan of a Lie "IndyWatch Feed Health"

From MediumDespite the Stanford prison experiments canonical status in intro psych classes around the country today, methodological criticism of it was swift and widespread in the years after it was conducted. Deviating from scientific protocol, Zimbardo and his students had published their first article about the experiment not in an academic journal of psychology but in The New York Times Magazine, sidestepping the usual peer review. Famed psychologist Erich Fromm, unaware that guards had been explicitly instructed to be tough, nonetheless opined that in light of the obvious pressures to abuse, what was most surprising about the experiment was how few guards did. The authors believe it proves that the situation alone can within a few days transform normal people into abject, submissive individuals or into ruthless sadists, Fromm wrote. It seems to me that the experiment proves, if anything, rather the contrary. Some scholars have argued that it wasnt an experiment at all. Leon Festinger, the psychologist who pioneered the concept of cognitive dissonance, dismissed it as a happening.

Article 

 

12:01

Are Americans a Broken People? "IndyWatch Feed Health"

From Alternet: A psychologist asks: Have consumerism, suburbanization and a malevolent corporate-government partnership so beaten us down that we no longer have the will to save ourselves?

Can people become so broken that truths of how they are being screwed do not set them free but instead further demoralize them? Has such a demoralization happened in the United States?

Do some totalitarians actually want us to hear how we have been screwed because they know that humiliating passivity in the face of obvious oppression will demoralize us even further?

What forces have created a demoralized, passive, dis-couraged U.S. population?

Can anything be done to turn this around?

Can people become so broken that truths of how they are being screwed do not set them free but instead further demoralize them?

Article 

 

 

12:00

Summer Dreams "IndyWatch Feed Health"

Note: This story is beautiful. Our kids are smart and deserve the benefit of the doubt at all times. Enjoy! ### The crowd was surprised when Sef Scott stepped up to the podium during his high school graduation ceremony last...

09:13

Grape seed proanthocyanidins protect against streptozotocininduced diabetic nephropathy. "IndyWatch Feed Health"

PMID:  Mol Med Rep. 2018 Jun 6. Epub 2018 Jun 6. PMID: 29901130 Abstract Title:  Grape seed proanthocyanidins protect against streptozotocininduced diabetic nephropathy by attenuating endoplasmic reticulum stressinduced apoptosis. Abstract:  Diabetic nephropathy (DN) is by far the most common cause of endstage renal disease (ESRD) in industrial countries, accounting for ~45% of all new ESRD cases in the United States. Grape seed proanthocyanidin extracts (GSPE) are powerful antioxidants, with an antioxidant ability 50fold greater than that of vitamin E and 20fold greater than that of vitamin C. The present study investigated whether GSPE can protect against streptozotocin (STZ)induced DN and aimed to elucidate a possible mechanism. Male Sprague Dawley rats were randomly divided into three groups: Control group (N), diabetes mellitus group (DM) injected with 40 mg/kg STZ, and theGSPE treatment group (intragastric administration of 250 mg/kg/day GSPE for 16 weeks after diabetes was induced in the rats). Blood and kidney samples were collected after treatment. The renal pathological changes were determined with periodic acidSchiff (PAS) staining, while the protein expression levels of glucoseregulated protein 78 (GRP78), phosphorylatedextracellular signalregulated kinase (pERK) and Caspase12 were determined by western blotting and immunohistochemical staining. Apoptosis was determined with a terminal deoxynucleotidyl transferase dUTP nickend labeling (TUNEL) assay. Compared with the DM group, the GSPE group had no significant changes in the blood urea nitrogen (BUN) level and serum creatinine (Scr) level, but showed a significant decline in the renal index (RI) level and 24h urinary albumin level (P

read more

09:09

Consuming a healthy diet that provides the recommended amount of magnesium can be an appropriate strategy for helping control blood pressure. "IndyWatch Feed Health"

PMID:  Int J Mol Sci. 2018 Jun 11 ;19(6). Epub 2018 Jun 11. PMID: 29891771 Abstract Title:  Role of Magnesium Deficiency in Promoting Atherosclerosis, Endothelial Dysfunction, and Arterial Stiffening as Risk Factors for Hypertension. Abstract:  Arterial hypertension is a disease with a complex pathogenesis. Despite considerable knowledge about this socially significant disease, the role of magnesium deficiency (MgD) as a risk factor is not fully understood. Magnesium is a natural calcium antagonist. It potentiates the production of local vasodilator mediators (prostacyclin and nitric oxide) and alters vascular responses to a variety of vasoactive substances (endothelin-1, angiotensin II, and catecholamines). MgD stimulates the production of aldosterone and potentiates vascular inflammatory response, while expression/activity of various antioxidant enzymes (glutathione peroxidase, superoxide dismutase, and catalase) and the levels of important antioxidants (vitamin C, vitamin E, and selenium) are decreased. Magnesium balances the effects of catecholamines in acute and chronic stress. MgD may be associated with the development of insulin resistance, hyperglycemia, and changes in lipid metabolism, which enhance atherosclerotic changes and arterial stiffness. Magnesium regulates collagen and elastin turnover in the vascular wall and matrix metalloproteinase activity. Magnesium helps to protect the elastic fibers from calcium deposition and maintains the elasticity of the vessels. Considering the numerous positive effects on a number of mechanisms related to arterial hypertension, consuming a healthy diet that provides the recommended amount of magnesium can be an appropriate strategy for helping control blood pressure.

read more

08:58

Ferulic acid also demonstrated a synergistic effect in combination with caspofungin against C. albicans. "IndyWatch Feed Health"

PMID:  J Food Drug Anal. 2018 Jan ;26(1):439-443. Epub 2017 Feb 13. PMID: 29389586 Abstract Title:  Evaluation of synergistic anticandidal and apoptotic effects of ferulic acid and caspofungin against Candida albicans. Abstract:  This study aimed to investigate the synergy between anticandidal and apoptotic effects of ferulic acid and caspofungin against Candida albicans and Candida glabrata, with the help of a quantitative checkerboard microdilution assay using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) as a viability dye. Apoptotic effects of caspofungin and ferulic acid concentrations (alone and combined) were analyzed for C. albicans and C. glabrata based on annexin V-propidium iodide binding capacities using flow cytometric analysis. C. albicans showed a synergistic effect, represented by a fractional inhibitory concentration index of0.5). Early and late apoptotic effects of caspofungin and ferulic acid concentrations (1 g/mL and 1000 g/mL) were calculated as 55.7% and 18.3%, respectively, while their necrotic effects were determined as 5.8% and 51.6%, respectively, using flow cytometric analyses. The apoptotic effects of the combination of caspofungin and ferulic acid at concentrations of 1 g/mL and 1000 g/mL on C. albicans and C. glabrata were 73.0% and 48.7%, respectively. Ferulic acid also demonstrated a synergistic effect in combination with caspofungin against C. albicans. Another possibility is to combine the existing anticandidal drug with phytochemicals to enhance the efficacy of anticandidal drug.

read more

08:53

Ferulic acid protects lipopolysaccharide-induced acute kidney injury. "IndyWatch Feed Health"

PMID:  Biomed Pharmacother. 2018 Apr ;100:304-315. Epub 2018 Feb 16. PMID: 29448207 Abstract Title:  Ferulic acid protects lipopolysaccharide-induced acute kidney injury by suppressing inflammatory events and upregulating antioxidant defenses in Balb/c mice. Abstract:  Sepsis-induced acute kidney injury (AKI) is responsible for 70-80% mortality in intensive care patients due to elevated levels of endotoxin, Lipopolysaccharide (LPS) caused by gram-negative infections. Ferulic acid (FA), a phenolic phytochemical is known for its renal protection on various induced models of nephrotoxicity. However, the curative effect of FA in LPS-induced AKI is not well studied. This study aimed to investigate the effect of FA on LPS-induced AKI in mice model and to understand the protective mechanisms involved, to provide evidence for FA in the treatment of AKI. Balb/c mice were treated with FA at 50mg/kg and 100mg/kg dosages after LPS stimulation (10mg/kg). At the end of the intervention, we determined the concentrations of serum creatinine and blood urea nitrogen, inflammatory cytokines and histopathological changes in animals. Also, the relative protein expression level of TLR4 mediated NF-B signaling pathway were studied in kidney tissues. FA treated animals showed upregulation of antioxidant defenses and suppression of inflammatory events by inhibiting TLR-4 mediated NFB activation. However, LPS alone administered group, resulted in rapid renal damage with increased levels of blood urea nitrogen and modest increase in creatinine; decreased antioxidant defenses and release of inflammatory cytokines. The histopathological analysis also revealed the protective action of the FA against sepsis induced fibrosis and renal damage. Our findings demonstrated that FA exhibitsmarked protective effects on LPS-induced AKI in mice suggesting its chemopotential role for treating AKI in humans.

read more

08:47

Mesenchymal stem cell-derived exosomes represent potentially new therapeutic agents in the therapy of degenerative and inflammatory ocular diseases. "IndyWatch Feed Health"

PMID:  Adv Exp Med Biol. 2018 May 18. Epub 2018 May 18. PMID: 29774506 Abstract Title:  Therapeutic Potential of Mesenchymal Stem Cell-Derived Exosomes in the Treatment of Eye Diseases. Abstract:  Mesenchymal stem cells (MSCs) were, due to their immunomodulatory and pro-angiogenic characteristics, extensively explored as new therapeutic agents in cell-based therapy of uveitis, glaucoma, retinal and ocular surface diseases.Since it was recently revealed that exosomes play an important role in biological functions of MSCs, herewith we summarized current knowledge about the morphology, structure, phenotype and functional characteristics of MSC-derived exosomes emphasizing their therapeutic potential in the treatment of eye diseases.MSC-derived exosomes were as efficient as transplanted MSCs in limiting the extent of eye injury and inflammation. Immediately after intravitreal injection, MSC-derived exosomes, due to nano-dimension, diffused rapidly throughout the retina and significantly attenuated retinal damage and inflammation. MSC-derived exosomes successfully delivered trophic and immunomodulatory factors to the inner retina and efficiently promoted survival and neuritogenesis of injured retinal ganglion cells. MSC-derived exosomes efficiently suppressed migration of inflammatory cells, attenuated detrimental Th1 and Th17 cell-driven immune response and ameliorated experimental autoimmune uveitis. MSC-derived exosomes were able to fuse with the lysosomes within corneal cells, enabling delivering of MSC-derived active-glucuronidase and consequent catabolism of accumulated glycosaminoglycans, indicating their therapeutic potential in the treatment of Mucopolysaccharidosis VII (Sly Syndrome). Importantly, beneficent effects were noticed only in animals that received MSC-derived exosomes and were not seen after therapy with fibroblasts-derived exosomes confirming specific therapeutic potential of MSCs and their products in the treatment of eye diseases.In conclusion, MSC-derived exosomes represent potentially new therapeutic agents in the therapy of degenerative and inflammatory ocular diseases.

read more

08:42

The antidepressant-like effects of ferulic acid involves the serotonergic and norepinergic systems. "IndyWatch Feed Health"

PMID:  Metab Brain Dis. 2015 Feb ;30(1):129-36. Epub 2014 Dec 9. PMID: 25483788 Abstract Title:  Antidepressant-like effects of ferulic acid: involvement of serotonergic and norepinergic systems. Abstract:  Ferulic acid is a polyphenol that has antioxidant, anti-inflammatory and anticancer properties. The present study analyzed the antidepressant-like potential of ferulic acid using two well-validated mouse models of despair test, tail suspension and forced swim tests. The results suggested that ferulic acid treatment at doses of 10, 20, 40 and 80 mg/kg (p.o.) significantly reduced the immobility time in both of these two tests. These doses that affected the depressive-like behaviors did now show any effect on locomotion counts. The further neurochemical assays suggested that ferulic acid increased monoamine neurotransmitter levels in the brain regions that are relative to mood disorders: the hippocampus and frontal cortex. The increased tend to serotonin and norepinephrine was also found in the hypothalamus after higher dose of ferulic acid treatment. The subsequent study suggested that monoamine oxidase A (MAO-A) activity was inhibited in the frontal cortex and hippocampus when treatment with 40 and 80 mg/kg ferulic acid; while MAO-B activity did not change significantly. The current study provides the first lines of evidence that serotonin and norepinephrine, but not dopamine levels were elevated in mouse hippocampus and frontal cortex after ferulic acid treatment. These changes may be attributable to the inhibition of MAO-A activities in the same brain regions.

read more

08:37

Ameliorative role of ferulic acid against diabetes associated oxidative stress induced spleen damage. "IndyWatch Feed Health"

PMID:  Food Chem Toxicol. 2018 May 11 ;118:272-286. Epub 2018 May 11. PMID: 29758315 Abstract Title:  Ameliorative role of ferulic acid against diabetes associated oxidative stress induced spleen damage. Abstract:  Spleen, a secondary lymphoid organ, is the site of initiation of most of the immune responses. The present study is centered on the ameliorative role of ferulic acid against diabetic complications in the spleen of male Wistar rats. Induction of diabetes by STZ (at a dose of 50mgkgbody wt, i.p.) reduced the spleen size, plasma insulin level, enhanced the blood glucose level and disrupted the intracellular antioxidant machineries along with the depletion of splenic white pulp. Induction of oxidative stress mediated inflammation and apoptosis (upregulation in the levels of inflammatory cytokines, translocation of NFB in the nucleus, alteration in Bax/Bcl-2 ratio, release of cytochrome c from mitochondria, activation of caspase-9 and 3, PARP cleavage and DNA fragmentation) were evidenced from immunoblot analyses, DNA fragmentation and TUNEL assay. However, ferulic acid administration post diabetes induction,(at a dose of 50mgkgbody wt, orally for eight weeks) could reverse such adverse effects. Therefore, ferulic acid, as a potential therapeutic agent may hold promise in evading oxidative stress mediated diabetic splenotoxicity in rats.

read more

08:31

Ferulic acid has the potential to reduce cardiovascular disease risk factors. "IndyWatch Feed Health"

PMID:  Nutrients. 2018 Jun 2 ;10(6). Epub 2018 Jun 2. PMID: 29865227 Abstract Title:  Ferulic Acid Supplementation Improves Lipid Profiles, Oxidative Stress, and Inflammatory Status in Hyperlipidemic Subjects: A Randomized, Double-Blind, Placebo-Controlled Clinical Trial. Abstract:  Ferulic acid is the most abundant phenolic compound found in vegetables and cereal grains. In vitro and animal studies have shown ferulic acid has anti-hyperlipidemic, anti-oxidative, and anti-inflammatory effects. The objective of this study is to investigate the effects of ferulic acid supplementation on lipid profiles, oxidative stress, and inflammatory status in hyperlipidemia. The study design is a randomized, double-blind, placebo-controlled trial. Subjects with hyperlipidemia were randomly divided into two groups. The treatment group (= 24) was given ferulic acid (1000 mg daily) and the control group (= 24) was provided with a placebo for six weeks. Lipid profiles, biomarkers of oxidative stress and inflammation were assessed before and after the intervention. Ferulic acid supplementation demonstrated a statistically significant decrease in total cholesterol (8.1%;= 0.001), LDL-C (9.3%;

read more

08:20

This study highlights that fucoidan might be served as an alternative therapeutic approach for the treatment of HBV infection. "IndyWatch Feed Health"

PMID:  Virol J. 2017 09 16 ;14(1):178. Epub 2017 Sep 16. PMID: 28915824 Abstract Title:  Fucoidan from Fucus vesiculosus suppresses hepatitis B virus replication by enhancing extracellular signal-regulated Kinase activation. Abstract:  BACKGROUND: Hepatitis B virus (HBV) infection is a serious public health problem leading to cirrhosis and hepatocellular carcinoma. As the clinical utility of current therapies is limited, the development of new therapeutic approaches for the prevention and treatment of HBV infection is imperative. Fucoidan is a natural sulfated polysaccharide that extracted from different species of brown seaweed, which was reported to exhibit various bioactivities. However, it remains unclear whether fucoidan influences HBV replication or not.METHODS: The HBV-infected mouse model was established by hydrodynamic injection of HBV replicative plasmid, and the mice were treated with saline or fucoidan respectively. Besides, we also tested the inhibitory effect of fucoidan against HBV infection in HBV-transfected cell lines.RESULTS: The result showed that fucoidan from Fucus vesiculosus decreased serum HBV DNA, HBsAg and HBeAg levels and hepatic HBcAg expression in HBV-infected mice. Moreover, fucoidan treatment also suppressed intracellular HBcAg expression and the secretion of the HBV DNA as well as HBsAg and HBeAg in HBV-expressing cells. Furthermore, we proved that the inhibitory activity by fucoidan was due to the activation of the extracellular signal-regulated kinase (ERK) pathway and the subsequent production of type I interferon. Using specific inhibitor of ERK pathway abrogated the fucoidan-mediated inhibition of HBV replication.CONCLUSION: This study highlights that fucoidan might be served as an alternative therapeutic approach for the treatment of HBV infection.

read more

08:14

A fucoidan-supplemented diet can inhibit DMBA-induced tumors in rats. "IndyWatch Feed Health"

PMID:  Nutr Cancer. 2017 Nov-Dec;69(8):1234-1244. Epub 2017 Oct 18. PMID: 29043842 Abstract Title:  The Protective and Immunomodulatory Effects of Fucoidan Against 7,12-Dimethyl benz[a]anthracene-Induced Experimental Mammary Carcinogenesis Through the PD1/PDL1 Signaling Pathway in Rats. Abstract:  Fucoidan is a sulfated polysaccharide that is extracted from brown algae seaweed. This study was designed to evaluate the protective and immunomodulatory effects of dietary fucoidan on 7,12-dimethyl benz[a]anthracene (DMBA)-induced experimental mammary carcinogenesis in rats. Sixty Sprague-Dawley rats were randomly assigned to four equal groups: the control group (control group), the cancer model group (model group), and the F1 and F2 groups, which were fed fucoidan at concentrations of 200 and 400 mg/kgbody weight, respectively. We found that fucoidan treatment decreased the tumor incidence and mean tumor weight and prolonged the tumor latency. Flow cytometric analyses revealed that the number of blood natural killer cells was higher after fucoidan treatment and that the proportions of CD4 and CD8 T cells were also increased. The serum levels of interleukin (IL)-6, IL-12p40, and interferon (IFN)- were higher in the rats treated with fucoidan compared to those of model rats. Moreover, the percentage of CD3+ Foxp3+ regulatory T cells in the blood and the levels of IL-10 and transforming growth factor in the serum were lower in the rats treated with fucoidan. Furthermore, fucoidan treatment decreased the expression of Foxp3 and programmed cell death 1 ligand 1 (PDL1) in tumor tissues. The levels of p-phosphatidyl inositol kinase 3 and p-AKT in tumor tissues were also lower than those of model rats. These results suggest that a fucoidan-supplemented diet can inhibit DMBA-induced tumors in rats. This study provides experimental evidence toward elucidating the immune enhancement induced by fucoidan through the programmed cell death 1/PDL1 signaling pathway. The immunomodulatory effect is one of the possible mechanisms of the protective effect of fucoidan against mammary carcinogenesis.

read more

08:02

Fucoidan may attenuate hyperoxic lung injury via the ERK1/2 signaling pathway. "IndyWatch Feed Health"

PMID:  Mol Med Rep. 2018 Jan ;17(1):1813-1818. Epub 2017 Nov 10. PMID: 29138816 Abstract Title:  Protective effects of fucoidan against hyperoxic lung injury via the ERK signaling pathway. Abstract:  High oxygen mechanical ventilation is widely used to treat various lung diseases; however, it may result in hyperoxia, which induces inflammation and lung injury. Fucoidan is an extract of the seaweed Fucus vesiculosus, which has previously been reported to exert effects against diabetic nephropathy. The present study is the first, to the best of our knowledge, to investigate the protective effects of fucoidan against hyperoxic lung injury. Balb/c mice were ventilated with 100% oxygen, with or without the atomization inhalation of fucoidan, for 36 h. Hyperoxia reduced the body weight and increased the relative lung weight of the mice. In addition, cell quantity and differentiation were determined using a hemocytometer, hyperoxia increased the total number of cells, and the number of macrophages, neutrophils and lymphocytes in the bronchoalveolar lavage fluid. Reverse transcriptionquantitative polymerase chain reaction (RTqPCR) demonstrated that hyperoxia also increased the mRNA expression levels of cluster of differentiation (CD)68, F4/80, CD64 and CD19 in lung tissue, and inducedlung morphological alterations. Furthermore, western blotting assay demonstrated that hyperoxia increased the expression levels of interleukin (IL)1, IL6 and tumor necrosis factor (TNF), and the phosphorylation of extracellular signalregulated kinase (ERK)1/2. Conversely, hyperoxiainduced inflammation and morphological alterations were significantly attenuated in the mice treated with fucoidan. Atomization inhalation of fucoidan also reduced the hyperoxiainduced expression of IL1, IL6 and TNF, and the phosphorylation of ERK1/2. These findings suggested that fucoidan may attenuate hyperoxic lung injury via the ERK1/2 signaling pathway.

read more

07:45

Fucoidan may be a suitable candidate chemopreventive agent in HT-29 colon cancer cells. "IndyWatch Feed Health"

PMID:  Oncol Rep. 2018 Mar ;39(3):1516-1522. Epub 2018 Jan 4. PMID: 29328495 Abstract Title:  Fucoidan downregulates insulin-like growth factor-I receptor levels in HT-29 human colon cancer cells. Abstract:  Fucoidan, a sulfated polysaccharide present in brown seaweed, has demonstrated anticancer activity in lung, breast, liver and colon cells. The insulin-like growth factor (IGF) signaling pathway regulates growth in HT-29 cells through the insulin receptor substrate-1 (IRS-1)/phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT) and Ras/Raf/extracellular signal-regulated kinase (ERK) pathways. The aim of the present study was to investigate whether fucoidandownregulates the IGF-IR signaling pathway in HT-29 human colon cancer cells. Fucoidan treatment (0-1,000 g/ml) was administered for 24 h in HT-29 cells. First, we investigated IRS-1/PI3K/AKT pathway-related protein expression levels following treatment with fucoidan (0-500 g/ml) using western blot analysis. Fucoidan significantly inhibited the expression of IGF-IR, PTEN, PI3K and AKT as well as their phosphorylated forms (p-IRS-1, p-PI3K and p-AKT). Next, we investigated the effects of fucoidan on Ras/Raf/ERK pathwayrelated protein expression levels in HT-29 cells. Fucoidan significantly inhibited the expression of IGF-IR, Shc, Ras, SOS, Raf and MEK. HT-29 cells were then incubated in the presence of fucoidan (0 or 250 g/ml), and IGF-I (10 nM) was added for 0 to 60 min. Immunoprecipitation (IP) experiments showed that fucoidan inhibited IGF-I-induced phosphorylation of IGF-IR, PI3K, Shc (IP, IGF-IR), and phosphorylated IRS-1 and PI3K (IP, IRS-1) compared to the control group. Western blot analysis showed that fucoidan inhibited the expression of IGF-I-induced p-IGF-IR/IGF-IR and p-AKT/AKT, but not p-ERK/ERK. In conclusion, the inhibition of cell viabilityby fucoidan in HT-29 cells may be due to the downregulation of IGF-IR signaling through the main IRS-1/PI3K/AKT pathway. Fucoidan also partially impacted Ras/Raf signaling in the Ras/Raf/ERK pathway. Therefore, we suggest that fucoidan may be a suitable candidate chemopreventive agent in HT-29 colon cancer cells.

read more

07:26

The study suggested the use of fucoidan as an intestinal flora modulator for potential prevention of breast cancer. "IndyWatch Feed Health"

PMID:  Food Funct. 2018 Feb 21 ;9(2):1214-1223. PMID: 29384543 Abstract Title:  The effect of fucoidan on intestinal flora and intestinal barrier function in rats with breast cancer. Abstract:  Recent research studies have shown that the intestinal flora are related to the occurrence and progress of breast cancer. This study investigates the effect of fucoidan on intestinal flora and intestinal barrier function in rats with 7,12-dimethylbenz[a]anthracene (DMBA)-induced breast cancers. Sixty female Sprague-Dawley rats were randomly assigned to the control group, the model group, and the F1 and F2 groups, which were fed fucoidan at concentrations of 200 and 400 mg per kg bw (body weight), respectively. Intestinal histopathological analysis was performed and 16S rDNA high-throughput sequencing was used to provide an overview of the intestinal flora composition. The contents of d-lactic acid (d-LA), diamine oxidase (DAO) and endotoxin in plasma were detected by ELISA. Expression levels of the tight junction (TJ) proteins, phosphorylated p38 MAPK and ERK1/2 were measured using western blotting. Our results suggested that the intestinal wall of the model group was damaged. However, after fucoidan intervention, the villi were gradually restored. ELISA showed that the levels of plasma endotoxin, d-LA and DAO decreased in the F1 and F2 groups compared to those in the model group. Fucoidan treatment also increased the expressions of ZO-1, occludin, claudin-1 and claudin-8. Furthermore, the expression levels of phosphorylated p38 MAPK and ERK1/2 were upregulated in fucoidan treatment groups. The results of 16S rDNA high-throughput sequencing indicated that fucoidan increased the diversity of the intestinal microbiota and induced changes in microbial composition, with the increased Bacteroidetes/Firmicutes phylum ratio. In conclusion, the supplement of fucoidan could improve the fecal microbiota composition and repair the intestinal barrier function. The study suggested the use of fucoidan as an intestinal flora modulator for potential prevention of breast cancer.

read more

07:12

Italys Populist Leader to End Forced Vaccinations, Poland Activates "IndyWatch Feed Health"

Source: Italys Populist Leader to End Forced Vaccinations, Poland Activates For more content like this visit REALfarmacy.com.

by JEFFEREY JAXEN A movement has broke through the surface globally with shocking momentum. Some see it as parental choice, others as an issue of informed consent, many in the medical community as a long-awaited return to the Hippocratic Oath. People from all walks of society and across multiple professions and classes are uniting in []

Source: Italys Populist Leader to End Forced Vaccinations, Poland Activates Learn more at REALfarmacy.com - Healthy News and Information.

06:58

Low molecular-weight fucoidan protects against hindlimb ischemic injury in type 2 diabetic mice. "IndyWatch Feed Health"

PMID:  J Diabetes. 2018 Apr 6. Epub 2018 Apr 6. PMID: 29633569 Abstract Title:  Low molecular-weight fucoidan protects against hindlimb ischemic injury in type 2 diabetic mice through enhancing endothelial nitric oxide synthase phosphorylation. Abstract:  BACKGROUND: Diabetes mellitus (DM) complications are associated with ischemic injury. Angiogenesis is a therapeutic strategy for diabetic foot. The aim of this study was to investigate the possible angiogenic effect of low molecular weight fucoidan (LMWF) in diabetic peripheral arterial disease (PAD).METHODS: Diabetic db/db mice and age-matched C57BL/6 mice underwent femoral artery ligation followed by LMWF (30, 60, 80mg/kg per day, p.o.) or cilostazol (30mg/kg/day, p.o.) treatment for 6weeks. Endothelium-dependent vasodilation and blood flow of the hindlimb were measured. Histological and western blot analyses of CD34, vascular endothelial growth factor (VEGF), eNOS, and inflammatory factors in the gastrocnemius were performed. The effects of LMWF were confirmed in human umbilical vein endothelial cells (HUVEC).RESULTS: Diabetic mice with ligation exhibited hindlimb ulceration, hydrosarca, and necrosis, increased expression of inflammatory factors, and decreased levels of VEGF and eNOS phosphorylation. Treatment with LMWF markedly ameliorated foot lesions, suppressed expression of inflammatory factors, and improved plantar perfusion by promoting endothelium-dependent vasodilation and revascularization in diabetic PAD mice. In high-glucose treated HUVEC, LMWF (40g/mL) reversed blunted endothelial cell proliferation, migration, and tube formation, and promoted eNOS phosphorylation and VEGF expression, whereas HUVEC pretreatment with 100mol/L N-nitro-l-arginine methyl ester, an eNOS antagonist, markedly inhibited the effects of LMWF.CONCLUSION: This study demonstrates that LMWF alleviates hindlimb ischemic damage, at least in part by promoting eNOS phosphorylation, nitric oxide production, and VEGF expression, resulting in enhanced angiogenesis in the ischemic region.

read more

06:46

Low molecular weight fucoidan attenuates experimental abdominal aortic aneurysm. "IndyWatch Feed Health"

PMID:  Mol Med Rep. 2018 May ;17(5):7089-7096. Epub 2018 Mar 16. PMID: 29568947 Abstract Title:  Low molecular weight fucoidan attenuates experimental abdominal aortic aneurysm through interfering the leukocyte-endothelial cells interaction. Abstract:  Low molecular weight fucoidan (LMWF) is a sulfated polysaccharide extracted from Saccharina Japonica that presents high affinity for P-selectin and abolish selectin-dependent recruitment of leukocytes. We hypothesized that dietary intake of LMWF, as a competitive binding agent of Pselectin, could limit the inflammatory infiltration and aneurysmal growth in an Angiotensin IIinduced abdominal aortic aneurysm (AAA) mouse model. The Gene Expression Omnibus database was used for gene expressions and gene set enrichment analysis. Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis showed that focal adhesion was involved in the development of AAA. However, dietary intake of LMWF could limit the enlargement of AAA, decreasing maximal aortic diameter and preserving elastin lamellae. Although LMWF did not decrease the circulatory monocytes count and lower the expression of Pselectin in endothelium, it reduced macrophages infiltration in media and adventitia. Furthermore, matrix metalloproteinase expression was markedly downregulated, accompanied with reduced expression of inflammatory mediators, including interleukin 1, tumor necrosis factorand monocyte chemotactic protein1. The present study revealed a novel target for the treatment of AAA and the antiinammatory effects of LMWF.

read more

06:33

The current results support the potential of combined fucoidan and cisplatin treatment as an effective therapeutic strategy in lung cancer. "IndyWatch Feed Health"

PMID:  Cancer Lett. 2018 May 7. Epub 2018 May 7. PMID: 29746926 Abstract Title:  Fucoidan Upregulates TLR4/CHOP-Mediated Caspase-3 and PARP Activation to Enhance Cisplatin-Induced Cytotoxicity in Human Lung Cancer Cells. Abstract:  Cisplatin-based therapy is a traditional, clinical treatment for cancers, including lung cancer. In this study, we found that sequential therapy, i.e., cisplatin followed by fucoidan, reduced tumor volume in an LLC1-bearing C57BL/6 mouse model. Using a series of combined therapeutic experiments, we found that the inhibition rate of the sequential treatment (cisplatinfucoidan) was 50 75%. However, the inhibition rate of the sequential treatment, with fucoidan pretreatment, was increased to 75 85%. Moreover, we found that the simultaneous administration of fucoidan and cisplatin synergistically inhibited lung cancer cell viability via inducing apoptotic responses, including upregulating cleaved caspase-3 and poly (ADP ribose) polymerase (PARP) expression. Mechanistically, we demonstrated that the fucoidan-induced, TLR4-mediated endoplasmic reticulum stress molecule CHOP promoted caspase-3 activation, which was further stimulated by the cisplatin-induced DNA damage responses, and CHOP shRNA eliminated fucoidan-induced caspase-3 cleavage but did not affect cisplatin-mediated apoptotic molecules. In addition, we observed an increasing number of clinical results that suggest combined cisplatin and fucoidan exerts a greater anti-tumorigenic effect in patients with lung cancer in Taiwan. Together, our current results support the potential of combined fucoidan and cisplatin treatment as an effective therapeutic strategy in lung cancer.

read more

06:21

Deuterium depleted water considerably suppressed cancer cell lines amoeboid movement in vitro. "IndyWatch Feed Health"

PMID:  J Trace Elem Med Biol. 2018 May 14. Epub 2018 May 14. PMID: 29773469 Abstract Title:  The effect of the deuterium depleted water on the biological activity of the eukaryotic cells. Abstract:  Here we show the dependence of the unicellular biosensor S.ambigua lifespan on the water D/H isotopic composition. This dependence is bell-shaped with descents both in case of deficiency or excess of deuterium in water. The influence of the water D/H isotopic composition on the cell culture proliferative potential and colony forming efficiency in vitro was tested on the human dermal fibroblasts. We observed that the deuterium depleted water stimulates cell colony formation at the early passages. The dynamics of the cell doubling index in the deuterium depleted water-based growth medium showed higher proliferation potential compared to the water with normal isotopic composition. Using scratch assay, we have also studied the impact of the growth medium D/H isotopic composition on the cell motility of human cancer cell lines A549 and HT29. We have shown that the deuterium depleted water considerably suppressed cancer cell lines amoeboid movement in vitro.

read more

06:01

Comcast Newsmakers: Fashion Rocks Autism "IndyWatch Feed Health"

Yolanda Vazquez speaks with Lisa Wiederlight, the Executive Director of SafeMinds, about the impact of Autism and their upcoming Fashion Rocks Autism event. The event is to be held on September 28th, 2018 from 7-10 p.m.

The post Comcast Newsmakers: Fashion Rocks Autism appeared first on SafeMinds.

04:47

A review of the microbiome-gut-brain axis and toll-like receptors in Parkinson's disease. "IndyWatch Feed Health"

PMID:  Int J Mol Sci. 2018 Jun 6 ;19(6). Epub 2018 Jun 6. PMID: 29882798 Abstract Title:  Microbiome-Gut-Brain Axis and Toll-Like Receptors in Parkinson's Disease. Abstract:  Parkinsons disease (PD) is a progressively debilitating neurodegenerative disease characterized by-synucleinopathy, which involves all districts of the brain-gut axis, including the central, autonomic and enteric nervous systems. The highly bidirectional communication between the brain and the gut is markedly influenced by the microbiome through integrated immunological, neuroendocrine and neurological processes. The gut microbiota and its relevant metabolites interact with the host via a series of biochemical and functional inputs, thereby affecting host homeostasis and health. Indeed, a dysregulated microbiota-gut-brain axis in PD might lie at the basis of gastrointestinal dysfunctions which predominantly emerge many years prior to the diagnosis, corroborating the theory that the pathological process is spread from the gut to the brain. Toll-like receptors (TLRs) play a crucial role in innate immunity by recognizing conserved motifs primarily found in microorganisms and a dysregulation in their signaling may be implicated in-synucleinopathy, such as PD. An overstimulation of the innate immune system due to gut dysbiosis and/or small intestinal bacterial overgrowth, together with higher intestinal barrier permeability, may provoke local and systemic inflammation as well as enteric neuroglial activation, ultimately triggering the development of alpha-synuclein pathology. In this review, we provide the current knowledge regarding the relationship between the microbiota-gutbrain axis and TLRs in PD. A better understanding of the dialogue sustained by the microbiota-gut-brain axis and innate immunity via TLR signaling should bring interesting insights in the pathophysiology of PD and provide novel dietary and/or therapeutic measures aimed at shaping the gut microbiota composition, improving the intestinal epithelial barrier function and balancing the innate immune response in PD patients, in order to influence the early phases of the following neurodegenerative cascade.

read more

04:41

Gut dysbiosis: a potential link between increased cancer risk in ageing and inflammaging. "IndyWatch Feed Health"

PMID:  Lancet Oncol. 2018 Jun ;19(6):e295-e304. Epub 2018 Jun 1. PMID: 29893261 Abstract Title:  Gut dysbiosis: a potential link between increased cancer risk in ageing and inflammaging. Abstract:  Cancer incidence substantially increases with ageing in both men and women, although the reason for this increase is unknown. In this Series paper, we propose that age-associated changes in gut commensal microbes, otherwise known as the microbiota, facilitate cancer development and growth by compromising immune fitness. Ageing is associated with a reduction in the beneficial commensal microbes, which control the expansion of pathogenic commensals and maintain the integrity of the intestinal barrier through the production of mucus and lipid metabolites, such as short-chain fatty acids. Expansion of gut dysbiosis and leakage of microbial products contributes to the chronic proinflammatory state (inflammaging), which negatively affects the immune system and impairs the removal of mutant and senescent cells, thereby enabling tumour outgrowth. Studies in animal models and the importance of commensals in cancer immunotherapy suggest that this status can be reversible. Thus, interventions that alter the composition of the gut microbiota might reduce inflammaging and rejuvenate immune functions to provide anticancer benefits in frail elderly people.

read more

04:30

Cardiac progenitor cellderived exosomes promote H9C2 cell growth via Akt/mTOR activation. "IndyWatch Feed Health"

PMID:  Int J Mol Med. 2018 May 21. Epub 2018 May 21. PMID: 29786755 Abstract Title:  Cardiac progenitor cellderived exosomes promote H9C2 cell growth via Akt/mTOR activation. Abstract:  Exosomes are cellderived vesicles released from a variety of mammalian cells that are involved in celltocell signalling. It has been reported that cardiac progenitor cells (CPCs) derived from an adult heart are one of the most promising stem cell types for cardioprotection and repair. The mammalian target of rapamycin (mTOR) signalling pathway is a pivotal regulator in CPCs, therefore, CPCderived exosomes were used in the present study to investigate whether it can promote H9C2 cell growth through the protein kinase B (PKB, or Akt)/mTOR signalling pathway. The CPCs were isolated from SpragueDawley hearts. Following treatment with a specific medium, the exosomes were purified and identified by electron micrograph and western blot assays, using CD63 and CD81 as markers. The methylthiazolyltetrazolium and 5ethynyl2'deoxyuridine methods were used to detect H9C2 cell growth. Theexpression of Akt and mTOR were detected by western blot analysis following treatment with 200 or 400 g/ml of exosomes for 24 or 48 h, respectively. It was found that, compared with higher concentrations of exosomes, prolonging the duration of exposure promoted cell growth. Accordingly, CPCderived exosomes stimulated the expression of Akt to a marked degree; groups treated with exosomes for 48 h showed higher expression of Akt than those treated for 24 h at the same concentration. mTOR was also stimulated by CPCderived exosomes. The activation of mTOR increased in accordance with the treatment time at an exosome concentration of 200 g/ml and decreased with treatment time at an exosome concentration of 400 g/ml. In conclusion, the present study demonstrated that CPCderived exosomes promoted H9C2 cell growth via the activation of Akt/mTOR in a timedependent manner at a relatively low exosome concentration, which may provide a novel therapy for cardiovascular disease.

read more

04:17

Beneficial effects of green tea catechins on neurodegenerative diseases. "IndyWatch Feed Health"

PMID:  Molecules. 2018 May 29 ;23(6). Epub 2018 May 29. PMID: 29843466 Abstract Title:  Beneficial Effects of Green Tea Catechins on Neurodegenerative Diseases. Abstract:  Tea is one of the most consumed beverages in the world. Green tea, black tea, and oolong tea are made from the same plant(L.) O. Kuntze. Among them, green tea has been the most extensively studied for beneficial effects on diseases including cancer, obesity, diabetes, and inflammatory and neurodegenerative diseases. Several human observational and intervention studies have found beneficial effects of tea consumption on neurodegenerative impairment, such as cognitive dysfunction and memory loss. These studies supported the basis of tea's preventive effects of Parkinson's disease, but few studies have revealed such effects on Alzheimer's disease. In contrast, several human studies have not reported these favorable effects with regard to tea. This discrepancy may be due to incomplete adjustment of confounding factors, including the method of quantifying consumption, beverage temperature, cigarette smoking, alcohol consumption, and differences in genetic and environmental factors, such as race, sex, age, and lifestyle. Thus, more rigorous human studies are required to understand the neuroprotective effect of tea. A number of laboratory experiments demonstrated the benefits of green tea and green tea catechins (GTCs), such as epigallocatechin gallate (EGCG), and proposed action mechanisms. The targets of GTCs include the abnormal accumulation of fibrous proteins, such as A and -synuclein, inflammation, elevated expression of pro-apoptotic proteins, and oxidative stress, which are associated with neuronal cell dysfunction and death in the cerebral cortex. Computational molecular docking analysis revealed how EGCG can prevent the accumulation of fibrous proteins. These findings suggest that GTCs have the potential to be used in the prevention and treatment of neurodegenerative diseases and could be useful for the development of new drugs.

read more

03:59

Exosomes in severe asthma: update in their roles and potential in therapy. "IndyWatch Feed Health"

PMID:  Biomed Res Int. 2018 ;2018:2862187. Epub 2018 May 8. PMID: 29854739 Abstract Title:  Exosomes in Severe Asthma: Update in Their Roles and Potential in Therapy. Abstract:  Exosomes are nanosized vesicles and have recently been recognized as important players in cell-to-cell communication. Exosomes contain different mediators such as proteins, nucleic acids (DNA, mRNA, miRNAs, and other ncRNAs), and lipid mediators and can shuttle their exosomal content to both neighboring and distal cells. Exosomes are very effective in orchestrating immune responses in the airways and all cell types can contribute to the systemic exosome pool. Intracellular communication between the broad range of cell types within the lung is crucial in disease emphasizing the importance of exosomes. In asthma, exosomes affect the inflammatory microenvironment which ultimately determines the development or alleviation of the pathological symptoms. Recent studies in this area have provided insight into the underlying mechanisms of disease and led to interest in using exosomes as potential novel therapeutic agents.

read more

03:45

Exosomes: a novel therapeutic target for Alzheimers disease? "IndyWatch Feed Health"

PMID:  Neural Regen Res. 2018 May ;13(5):930-935. PMID: 29863025 Abstract Title:  Exosomes: a novel therapeutic target for Alzheimer's disease? Abstract:  Extracellular exosomes are formed inside the cytoplasm of cells in compartments known as multivesicular bodies. Thus, exosomes contain cytoplasmic content. Multivesicular bodies fuse with the plasma membrane and release exosomes into the extracellular environment. Comprehensive research suggests that exosomes act as both inflammatory intermediaries and critical inducers of oxidative stress to drive progression of Alzheimer's disease. An important role of exosomes in Alzheimer's disease includes the formation of neurofibrillary tangles and beta-amyloid production, clearance, and accumulation. In addition, exosomes are involved in neuroinflammation and oxidative stress, which both act as triggers for beta-amyloid pathogenesis and tau hyperphosphorylation. Further, it has been shown that exosomes are strongly associated with beta-amyloid clearance. Thus, effective measures for regulating exosome metabolism may be novel drug targets for Alzheimer's disease.

read more

03:29

Immunomodulatory function of Treg-derived exosomes is impaired in patients with relapsing-remitting multiple sclerosis. "IndyWatch Feed Health"

PMID:  Immunol Res. 2018 Jun 7. Epub 2018 Jun 7. PMID: 29882035 Abstract Title:  Immunomodulatory function of Treg-derived exosomes is impaired in patients with relapsing-remitting multiple sclerosis. Abstract:  Multiple sclerosis (MS) is an autoimmune disease which is characterized by neuroaxonal degeneration in the central nervous system. Impaired function of regulatory T cells (Tregs) is believed to be an underlying pathogenic mechanism in MS. Tregs is able to release exosomes, which contain a considerable amount of protein and RNA. Exosomes are capable of transporting their content to other cells where the released content exerts biological functions. Here, we investigated whether Tregs exosomes of RRMS patients or healthy controls might regulate the proliferation or survival of T lymphocytes. Regulatory T cells derived from MS patients or healthy controls were cultured for 3 days and exosomes were purified from supernatants. Treg-derived exosomes were co-cultured with conventional T cells (Tconv). The percentages of Tconv proliferation and apoptosis were measured. Our findings showed that the percentage of proliferation suppression induced by exosomes in patients compared to healthy controls was 8.041.17 and 12.51.22, respectively, p value=0.035. Moreover, the rate of Tconv apoptosis induced by exosome of MS patient was less than healthy controls (0.680.12 vs. 1.290.13; p value=0.015). Overall, Treg-derived exosomes from MS patients and healthy controls suppressed the proliferation and induced apoptosis in Tconv. However, the effect of MS-derived exosomes was significantly less than healthy controls. Our results point to an alternative Treg inhibitory mechanism which might be important in immunopathogenesis of MS. Although, the cause of the exosomal defect in MS patients is unclear, manipulation of patients' Treg-derived exosomes to restore their suppressive activity might be considered as a potential therapeutic approach. Graphical abstract .

read more

03:23

These data demonstrated that ECFC-derived exosomes have beneficial effects on blood-brain barrier integrity in mice with traumatic brain injury. "IndyWatch Feed Health"

PMID:  Exp Neurol. 2018 Jun 6 ;307:99-108. Epub 2018 Jun 6. PMID: 29883579 Abstract Title:  Endothelial colony-forming cell-derived exosomes restore blood-brain barrier continuity in mice subjected to traumatic brain injury. Abstract:  Traumatic brain injury (TBI) tends to cause disruption of the blood-brain barrier (BBB). Previous studies have shown that intravenously or intracerebroventricularly infused human umbilical cord blood-derived endothelial colony-forming cells (ECFCs) can home to injury sites and improve outcomes in mice subjected to experimental TBI. Several reports have demonstrated that these cells did not incorporate directly into newly formed vasculature but instead stimulated the proliferation and migration of tissue-resident endothelial cells (ECs) via paracrine mechanisms. In the present study, exosomes, which range from 30 to 150nm in diameter, were isolated from ECFC-conditioned medium. The exosomes were labeled with PKH67 ex vivo, and we observed that they were taken up by ECs with high efficiency after 12h of incubation. Pretreatment with ECFC-derived exosomes promoted the migration of ECs subjected to scratch injury, and incubating ECs exposed to hypoxia with ECFC-derived exosomes decreased PTEN expression, stimulated AKT phosphorylation and increased tight junction (TJ) protein expression in the cells. Furthermore, in vivo delivery of ECFC-derived exosomes into TBI mice also inhibited PTEN expression and increased AKT expression, changes accompanied by reductions in Evans blue (EB) dye extravasation, brain edema and TJ degradation. These data demonstrated that ECFC-derived exosomes have beneficial effects on BBB integrity in mice with TBI.

read more

03:18

These findings suggest MDA231-LM2-secreted exosomes as a promising target to treat breast cancer lung metastasis. "IndyWatch Feed Health"

PMID:  Biochem Biophys Res Commun. 2018 Jun 11. Epub 2018 Jun 11. PMID: 29885840 Abstract Title:  Exosomes derived from breast cancer lung metastasis subpopulations promote tumor self-seeding. Abstract:  Lung metastasis is a primary obstacle in the clinical treatment of metastatic breast cancer. Most patients with lung metastasis eventually die of recurrence. Recurrence may be related to self-seeding, which occurs when circulating tumor cells re-seed into the tumors they originated from (metastasis or carcinoma in situ). Tumor-derived exosomes have been intensively revealed to promote the progression of various cancers. However, whether tumor-derived exosomes play roles in tumor self-seeding has not yet been identified. By establishing a self-seeding nude mouse model, we found that exosomes derived from MDA231-LM2 cells (subpopulations of breast cancer lung metastasis) potentiate the growth of MDA-MB-231 xenografts. More importantly, laser confocal microscopy and flow cytometry results identified that MDA231-LM2-secreted exosomes promote the seeding of MDA231-LM2 cells into MDA-MB-231 xenografts. These findings suggest MDA231-LM2-secreted exosomes as a promising target to treat breast cancer lung metastasis.

read more

03:03

Exosomes derived from human induced pluripotent stem cells ameliorate the aging of skin fibroblasts. "IndyWatch Feed Health"

PMID:  Int J Mol Sci. 2018 Jun 9 ;19(6). Epub 2018 Jun 9. PMID: 29890746 Abstract Title:  Exosomes Derived from Human Induced Pluripotent Stem Cells Ameliorate the Aging of Skin Fibroblasts. Abstract:  Stem cells and their paracrine factors have emerged as a resource for regenerative medicine. Many studies have shown the beneficial effects of paracrine factors secreted from adult stem cells, such as exosomes, on skin aging. However, to date, few reports have demonstrated the use of exosomes derived from human pluripotent stem cells for the treatment of skin aging. In this study, we collected exosomes from the conditioned medium of human induced pluripotent stem cells (iPSCs) and investigated the effect on aged human dermal fibroblasts (HDFs). Cell proliferation and viability were determined by an MTT assay and cell migration capacity was shown by a scratch wound assay and a transwell migration assay. To induce photoaging and natural senescence, HDFs were irradiated by UVB (315 nm) and subcultured for over 30 passages, respectively. The expression level of certain mRNAs was evaluated by quantitative real-time PCR (qPCR). Senescence-associated--galactosidase (SA--Gal) activity was assessed as a marker of natural senescence. As a result, we found that exosomes derived from human iPSCs (iPSCs-Exo) stimulated the proliferation and migration of HDFs under normal conditions. Pretreatment with iPSCs-Exo inhibited the damages of HDFs and overexpression of matrix-degrading enzymes (MMP-1/3) caused by UVB irradiation. The iPSCs-Exo also increased the expression level of collagen type I in the photo-aged HDFs. In addition, we demonstrated that iPSCs-Exo significantly reduced the expression level of SA--Gal and MMP-1/3 and restored the collagen type I expression in senescent HDFs. Taken together, it is anticipated that these results suggest a therapeutic potential of iPSCs-Exo for the treatment of skin aging.

read more

02:55

Exosomes, the message transporters in vascular calcification. "IndyWatch Feed Health"

PMID:  J Cell Mol Med. 2018 Jun 12. Epub 2018 Jun 12. PMID: 29892998 Abstract Title:  Exosomes, the message transporters in vascular calcification. Abstract:  Vascular calcification (VC) is caused by hydroxyapatite deposition in the intimal and medial layers of the vascular wall, leading to severe cardiovascular events in patients with hypertension, chronic kidney disease and diabetes mellitus. VC occurrences involve complicated mechanism networks, such as matrix vesicles or exosomes production, osteogenic differentiation, reduced cell viability, aging and so on. However, with present therapeutic methods targeting at VC ineffectively, novel targets for VC treatment are demanded. Exosomes are proven to participate in VC and function as initializers for mineral deposition. Secreted exosomes loaded with microRNAs are also demonstrated to modulate VC procession in recipient vascular smooth muscle cells. In this review, we targeted at the roles of exosomes during VC, especially at their effects on transporting biological information among cells. Moreover, we will discuss the potential mechanisms of exosomes in VC.

read more

02:37

Resveratrol induces autophagy-dependent apoptosis in HL-60 cells. "IndyWatch Feed Health"

PMID:  BMC Cancer. 2018 May 22 ;18(1):581. Epub 2018 May 22. PMID: 29788929 Abstract Title:  Resveratrol induces autophagy-dependent apoptosis in HL-60 cells. Abstract:  BACKGROUND: All known mechanisms of apoptosis induced by resveratrol act through cell cycle arrest and changes in mitochondrial membrane potential. It is currently unknown whether resveratrol-induced apoptosis is associated with other physiological processes, such as autophagy.METHODS: Apoptosis-related markers involved in the intrinsic and extrinsic apoptotic pathways, and autophagic markers were detected by using western blotting and immunofluorescence. Mitochondrial membrane potential was assayed by flow cytometry. Pharmaceutical or genetic inhibition of autophagy involved were carried by 3- methyladenine or knockdown of autophagy-related (Atg) genes by siRNA. Differences between two values were tested by Student's unpaired t test.RESULTS: We show that resveratrol-induced apoptosis occurs through both the intrinsic and extrinsic apoptotic pathways. Mitochondrial membrane potential and apoptosis-related markers, such as an increased Bax/Bcl-2 ratio, and cleaved forms of caspase-8 and caspase-3, arise following resveratrol addition. Moreover, we find that resveratrol increases both the levels of microtubule-associated protein 1 light chain 3-II and the number of autophagosomes, and further demonstrate that resveratrol-induced autophagy depends on the LKB1-AMPK-mTOR pathway. We next reveal that some apoptosis-related markers induced by resveratrol are further attenuated by the inhibition of autophagy with 3-methyladenine or knockdown of autophagy-related (Atg) genes by siRNA.CONCLUSIONS: These results suggest that resveratrol induced apoptotic cell death of HL-60 cells depends on the autophagy activated through both the LKB1-AMPK and PI3K/AKT-regulated mTOR signaling pathways.

read more

02:30

Resveratrol prevents high-calorie diet-induced learning and memory dysfunction. "IndyWatch Feed Health"

PMID:  Neurol Res. 2018 May 24:1-7. Epub 2018 May 24. PMID: 29792387 Abstract Title:  Resveratrol prevents high-calorie diet-induced learning and memory dysfunction in juvenile C57BL/6J mice. Abstract:  OBJECTIVE: Because resveratrol (RSV) has been shown to improve learning and memory, so we investigated the potential benefit of RSV on learning and memory deficits in juvenile mice fed with a HC diet and explored the molecular mechanisms underlying this process.METHODS: Six-week-old C57BL/6J mice were divided into three different diet groups: control, HC diet, and HC + RSV diet. Serum insulin and insulin-like growth factor 1 (IGF-1) levels were measured using enzyme-linked immunosorbent assays. Protein expression was examined by immunohistochemistry and western blotting.RESULTS: Administration of RSV daily (30 mg/kg) prevented the HC diet-induced increase in juvenile animal body weight but did not improve any other physiological conditions, including fasting blood glucose and serum cholesterol, triglyceride, insulin, and IGF-1 levels. However, RSV did prevent learning and memory deficits in the HC group. Peroxisome proliferator-activated receptor gamma (PPAR) was downregulated in the CA1 region of the hippocampus in both the HC and HC + RSV groups, but the reduction was significantly greater in the HC + RSV group (P 

read more

02:19

EGCG promotes atherosclerotic lesion stability in apolipoprotein E-deficient mice. "IndyWatch Feed Health"

PMID:  Kardiol Pol. 2018 Jun 4. Epub 2018 Jun 4. PMID: 29862488 Abstract Title:  Green tea polyphenol epigallocatechin-3-gallate increases atherosclerotic plaque stability in apolipoprotein E-deficient mice fed a high-fat diet. Abstract:  BACKGROUND: Epigallocatechin-3-gallate (EGCG), which is the principal component of green tea, has been shown to prevent the formation of atherosclerosis. However, the effect of EGCG on atherosclerotic plaque stability remains unknown.AIM: This study aimed to assess whether EGCG can enhance atherosclerotic plaque stability and to investigate the underlying mechanisms.METHODS: Apolipoprotein E-deficient mice fed a high-fat diet were injected intraperitoneally with EGCG (10 mg/kg ) for 16 weeks. Cross sections of the brachiocephalic arteries were stained with hematoxylin and eosin (HE) for morphometric analyses or Masson's trichrome for collagen content analyses. Immunohistochemistry was performed to evaluate the percentage of macrophages and smooth muscle cells (SMCs). Protein expression and matrix metalloproteinase (MMP) activity were assayed by Western blot and gelatin zymography, respectively. Serum inflammatory cytokine levels were quantified by enzyme-linked immunosorbent assay.RESULTS: After 16 weeks of feeding the high-fat diet, there was clear atherosclerosis formation in the proximal brachiocephalic artery segments according to HE staining. EGCG treatment significantly increased the thickness of the fibrous cap. In the atherosclerotic plaques of the EGCG group, the relative macrophage content was decreased, whereas the relative SMC and collagen contents were increased. The expression levels of MMP-2, MMP-9 and extracellular matrix metalloproteinase inducer (EMMPRIN) were significantly decreased by EGCG treatment. In addition, EGCG treatment decreased the circulating TNF-a, IL-6, MCP-1 and IFN- levels in apolipoprotein E-deficient mice.CONCLUSIONS: EGCG promotes atherosclerotic lesion stability in apolipoprotein E-deficient mice. Potentially, these effects are mediated through the inhibition of inflammatory cytokine, MMPs and EMMPRIN expression.

read more

02:08

EGCG reversed GIO in rats by protecting osteoblasts by activating the Nrf2 signaling pathway. "IndyWatch Feed Health"

PMID:  Front Pharmacol. 2018 ;9:447. Epub 2018 May 9. PMID: 29867459 Abstract Title:  Epigallocatechin-3-Gallate Ameliorates Glucocorticoid-Induced Osteoporosis of Ratsand. Abstract:  Prolonged administration of overdoses of glucocorticoids results in increased bone remodeling, leading to glucocorticoid-induced osteoporosis (GIO), which is primarily due to the dysfunction and apoptosis of osteoblasts. The present study investigated the therapeutic effect and molecular mechanism of action of epigallocatechin-3-gallate (EGCG), a bioactive catechin in green tea, in high-dose dexamethasone-induced osteoblast differentiationand.The anti-dexamethasone (DEX) effects of EGCG on primary osteoblasts were determined on the basis of cell viability and alkaline phosphatase (ALP) and total cellular superoxide dismutase (SOD) activities. Flow cytometry and Western blot analysis were also used to evaluate the expression of related biomarkers, and bone microarchitecture was also extensively examined in a rat model.The results showed that EGCG pretreatment significantly increased osteoblast viability and ALP and SOD activities when cells were exposed to DEX. Alizarin red staining indicated that there was more mineralization with EGCG pretreatment, countering DEX effects. EGCG reduced DEX-induced reactive oxygen species at both the mitochondrial and cellular levels in osteoblasts by activating the nuclear factor erythroid-derived 2-like-2 (Nrf2) pathway. In addition, EGCG protected osteoblasts from apoptosis. EGCG also regulated the formation of active glucocorticoid by 11-hydroxysteroid dehydrogenase activity. Furthermore, femoral micro-computed tomography scans revealed that EGCG improved bone microstructure and mitigated DEX-induced deterioration of bone quality.These findings suggested that EGCG reversed GIO in rats by protecting osteoblasts by activating the Nrf2 signaling pathway.

read more

01:48

Resveratrol interrupts the pro-invasive communication between cancer associated fibroblasts and cholangiocarcinoma cells. "IndyWatch Feed Health"

PMID:  Cancer Lett. 2018 Aug 28 ;430:160-171. Epub 2018 May 23. PMID: 29802929 Abstract Title:  Resveratrol interrupts the pro-invasive communication between cancer associated fibroblasts and cholangiocarcinoma cells. Abstract:  Cholangiocarcinoma (CCA), the cancer arising from the epithelial cells of bile ducts, is a prototype of inflammatory-driven cancer. Cytokines released by cancer associated fibroblasts (CAFs) play a pivotal role in CCA progression, driving the epigenetic Epithelial-to-Mesenchymal transition and the growth and metastasization of CCA cells. Consistently, the conditioned medium from CCA-derived CAFs further stimulated the secretion of IL-6, and to a lesser extent of IL-8, by CCA cells. CCA has a poor prognosis, because of late diagnosis and of high resistance to radio- and chemo-therapy of CCA cells. Targeting the CAFs and their secretion could be an alternative option. We found that while IL-6 indeed promoted the cell migration of invasive CCA cells, the nutraceutical Resveratrol strongly counteracted this effect both in CCA cells and in immortalized cholangiocytes. More importantly, here we show that Resveratrol has the potential to abrogate the secretion of IL-6 by CAFs. While the conditioned medium from CAFs strongly induced IL-6 mediated motility of CCA cells, the conditioned medium from CAFs pre-treated with Resveratrol completely halted cancer cell motility and reverted the N-to E-cadherin switch in migrating cells. This effect was associated with stimulation of autophagy in the cancer cells. This is the first demonstration that CAFs secretory products directly affect the regulation of autophagy and consequently the behavior of CCA cells, and that a nutraceutical may revert the malignant phenotype of cancer cells by acting on CAFs metabolism and secretion.

read more

01:35

Resveratrol inhibits pulmonary fibrosis by regulating miR-21 through MAPK/AP-1 pathways. "IndyWatch Feed Health"

PMID:  Biomed Pharmacother. 2018 May 26 ;105:37-44. Epub 2018 May 26. PMID: 29843043 Abstract Title:  Resveratrol inhibits pulmonary fibrosis by regulating miR-21 through MAPK/AP-1 pathways. Abstract:  OBJECTIVE: To explore the molecular mechanism of Res in regulation of pulmonary fibrosis (PF).METHODS: Rats were injected with bleomycin (BLM) to establish a PF model and treated with resveratrol (Res) and/or miR-21 agomir. After 14 days, lung tissues were collected for Hematoxylin-eosin and Masson's staining, and real-time quantitative polymerase chain reaction and Western blot were performed to detect fibrosis-related protein expression and the activation of the TGF-1/Smad pathway. In vitro, MRC-5 cells were pretreated with TGF-1, Res, and/or miR-21 agomir. After 48h, total soluble collagen was detected with a Sircol Soluble Collagen Assay. Subsequently, a miR-21 mimic was transfected into MRC-5 cells, and a luciferase reporter assay was employed to verify whether miR-21 targeted Smad7.RESULTS: Res reversed the increased levels of miR-21 induced by BLM and alleviated serious PF symptoms, but agomiR-21 treatment effectively impaired the above manifestations. In vivo, miR-21 inhibited the decreases of TGF-1 and p-Smad2/3 that were induced by Res. In vitro, miR-21 significantly disrupted the positive effect of Res on TGF--induced collagen deposition, as well as the levels of Fn, -SMA, p-Smad2, and Smad7. In addition, Smad7 was found to be a direct target of miR-21-5p. TGF- stimulation led to an enormous increase in p-c-Jun, c-Jun, and c-Fos, which were significantly reduced by Res. Finally, miR-21 sharply reduced the increased phosphorylation levels of ERK, JNK and p38 that were induced by Res.CONCLUSION: Res inhibits BLM-induced PF by regulating miR-21 through MAPK/AP-1 pathways.

read more

01:21

Resveratrol protects murine chondrogenic ATDC5 cells against LPS-induced inflammatory injury. "IndyWatch Feed Health"

PMID:  Cell Physiol Biochem. 2018 May 24 ;47(3):972-980. Epub 2018 May 24. PMID: 29843156 Abstract Title:  Resveratrol Protects Murine Chondrogenic ATDC5 Cells Against LPS-Induced Inflammatory Injury Through Up-Regulating MiR-146b. Abstract:  BACKGROUND/AIMS: Resveratrol (RSV) has been reported as a promising oral supplementation for osteoarthritis treatment, while the mechanism of its action is still unclear. The specific aim of this study is to decode one of the mechanisms by which RSV protects chondrocyte.METHODS: Mouse chondrogenic cell line ATDC5 was treated with 30M RSV for 24 h, and 10 g/ml LPS for 12 h, after which cell viability, apoptosis, and the release of pro-inflammatory cytokines were assessed. The expression of miR-146b in ATDC5 cells was silenced by the specific inhibitor transfection, and then cell viability, apoptosis and inflammation were re-assessed.RESULTS: The IC50 value of LPS in ATDC5 cells was about 10.27g/ml. LPS with a dosage of 10 g/ml repressed cell viability, induced apoptosis, and increased the release of IL-1, IL-6 and TNF-. RSV pre-treatment (30 M) significantly alleviated LPS-induced apoptosis and inflammation. More importantly, miR-146b was up-regulated by RSV, and the protectivefunctions of RSV on ATDC5 cells were attenuated by miR-146b silence. Further, NF-B and p38MAPK pathways were activated by LPS, and were deactivated by RSV. Besides, RSV-induced the deactivation of NF-B and p38MAPK pathways was reversed by miR-146b silence.CONCLUSIONS: Our findings suggest that RSV protects ATDC5 cells from LPS-induced inflammatory and apoptotic injury via up-regulation of miR-146b and thereby deactivation of NF-B and p38MAPK pathways.

read more

01:11

Upcoming Northern California Climate Engineering Awareness Event "IndyWatch Feed Health"

GeoengineeringWatch.org 445731On Saturday, July 28th, 2018, a critical public awareness event has been scheduled at the Shasta County Fairgrounds in Anderson, California (1890 Briggs St). The event will be in the Fusaro Hall which has seating for approximately 800. Verifiable evidence will be presented to the public on the ongoing global climate engineering / geoengineering  programs that are wreaking havoc on the atmosphere, environment, and

00:51

These findings suggest that resveratrol may potentially prevent arterial restenosis. "IndyWatch Feed Health"

PMID:  Mol Med Rep. 2018 May 23. Epub 2018 May 23. PMID: 29845301 Abstract Title:  Resveratrol inhibits angiotensin IIinduced proliferation of A7r5 cells and decreases neointimal hyperplasia by inhibiting the CaMKIIHDAC4 signaling pathway. Abstract:  Resveratrol has been reported to inhibit vascular smooth muscle cell proliferation and neointimal hyperplasia following arterial injury; however, the underlying mechanisms remain unclear. The present study was designed to investigate the effects of resveratrol on angiotensin II (AngII)induced proliferation of A7r5 cells and explore the molecular mechanisms responsible for the observed effects. Resveratrol inhibited cell proliferation and migration, and decreased the AngIIinduced protein expression of smooth muscle actin (SMA), proliferating cell nuclear antigen (PCNA) and cyclindependent kinase 4 (CDK4). Resveratrol inhibited AngIIinduced activation of intracellular Ca2+/calmodulindependent protein kinase II (CaMKII) and histone deacetylases 4 (HDAC4), as well as blocking AngIIinduced cell cycle progression from the G0/G1 to Sphase. In vivo, 4weeks of resveratrol treatment decreased the neointima area and the neointima/media area ratio in rats following carotid balloon injury. Resveratrol also inhibited the protein expression of total and phosphorylated CaMKII and HDAC4 in the injured arteries. In conclusion, the present study demonstrated that resveratrol attenuated AngIIinduced cell proliferation and neointimal hyperplasia by inhibiting the CaMKIIHDAC4 signaling pathway. These findings suggest that resveratrol may potentially prevent arterial restenosis.

read more

00:17

Resveratrol improves tube formation in AGE-induced late endothelial progenitor cells by suppressing syndecan-4 shedding. "IndyWatch Feed Health"

PMID:  Oxid Med Cell Longev. 2018 ;2018:9045976. Epub 2018 Apr 10. PMID: 29849922 Abstract Title:  Resveratrol Improves Tube Formation in AGE-Induced Late Endothelial Progenitor Cells by Suppressing Syndecan-4 Shedding. Abstract:  Dysfunction of endothelial progenitor cells (EPCs) contributes to cardiovascular complications in diabetes, and resveratrol has been shown to improve EPC functions. Syndecan-4 (Synd4), a cell surface heparin sulfate proteoglycan, has been shown to promote neovascularization. Thus, the present study was performed to determine whether resveratrol promoted angiogenesis of EPCs by regulating Synd4. Late EPCs were isolated from human peripheral blood and stimulated with AGEs. Western blot showed that AGEs induced Synd4 shedding in a dose- and time-dependent manner. AGE-induced Synd4 shedding was partly reversed by NAC or resveratrol, along with normalized ROS production. Overexpression of Synd4 or pretreatment of resveratrol reversed AGE-impaired tube formation of EPCs and regulated the Akt/eNOS pathway. Furthermore, resveratrol suppressed Synd4 shedding via the inhibition of oxidative stress and improved tube formation of late EPCs via the regulation of the Synd4/Akt/eNOS pathway.

read more

00:02

Mechanism of resveratrol in improving ovarian function in a rat model of premature ovarian insufficiency. "IndyWatch Feed Health"

PMID:  J Obstet Gynaecol Res. 2018 May 30. Epub 2018 May 30. PMID: 29851197 Abstract Title:  Mechanism of resveratrol in improving ovarian function in a rat model of premature ovarian insufficiency. Abstract:  AIM: To investigate the mechanism of resveratrol treatment in chemically induced premature ovarian insufficiency (POI) in rats.METHODS: Five-week-old specific pathogen-free healthy Sprague-Dawley female rats (n=90) were randomly divided into five groups (n=18): low-dose resveratrol (group A), moderate-dose resveratrol (group B), high-dose resveratrol (group C), model control group (group D) and blank control group (group E). POI was induced in rats from the resveratrol-treated groups and the model control group according to observed indexes using a previously established model (oral administration of tripterygium wilfordii polyglycoside tablet 50mg/kg/day for 14days). Western blot analysis was performed to compare levels of PI3K, p-PI3K, Akt, p-Akt, mTOR, p-mTOR, Bax, Bcl-2 and Caspase-3 in ovarian tissues of each group, and expression of p-Akt, p-mTOR, Bax, Bcl-2 and Caspase-3 was also evaluated by immunohistochemistry. Serum levels of malondialdehyde (MDA) and superoxide dismutase (SOD) were determined using enzyme-linked immunosorbent assay.RESULTS: Compared with controls, we found that serum MDA decreased and SOD increased in resveratrol-treated groups. In addition, we found increased expression of p-PI3K, p-Akt, p-mTOR and Bcl-2, and decreased expression of Bax and Caspase-3 were observed in ovarian tissues of treated rats with POI. There was reduced ovarian function in POI rats compared with rats from the blank control group.CONCLUSION: Resveratrol reduced oxidative stress and inhibited apoptosis in granulosa cells by activating the PI3K/Akt/mTOR signaling pathway in a rat model of POI.

read more

Friday, 15 June

23:42

Resveratrol inhibits ROS-promoted activation and glycolysis of pancreatic stellate cells via suppression of miR-21. "IndyWatch Feed Health"

PMID:  Oxid Med Cell Longev. 2018 ;2018:1346958. Epub 2018 Apr 26. PMID: 29854071 Abstract Title:  Resveratrol Inhibits ROS-Promoted Activation and Glycolysis of Pancreatic Stellate Cells via Suppression of miR-21. Abstract:  Activation of pancreatic stellate cells (PSCs) initiates pancreatic fibrosis in chronic pancreatitis and furnishes a niche that enhances the malignancy of pancreatic cancer cells (PCCs) in pancreatic ductal adenocarcinoma (PDAC). Resveratrol (RSV), a natural polyphenol, exhibits potent antioxidant and anticancer effects. However, whether and how RSV influences the biological properties of activated PSCs and the effects of these changes on tumor remain unknown. In the present study, we found that RSV impeded hydrogen peroxide-driven reactive oxygen species- (ROS-) induced activation, invasion, migration, and glycolysis of PSCs. In addition, miR-21 expression in activated PSCs was downregulated after RSV treatment, whereas the PTEN protein level increased. miR-21 silencing attenuated ROS-induced activation, invasion, migration, and glycolysis of PSCs, whereas the overexpression of miR-21 rescued the responses of PSCs treated with RSV. Moreover, RSV or N-acetyl-L-cysteine (NAC) administration or miR-21 knockdown in PSCs reduced the invasion and migration of PCCs in coculture, and the effects of RSV were partly reversed by miR-21 upregulation. Collectively, RSV inhibits PCC invasion and migration through suppression of ROS/miR-21-mediated activation and glycolysis in PSCs. Therefore, targeting miR-21-mediated glycolysis by RSV in tumor stroma may serve as a new strategy for clinical PDAC prevention or treatment.

read more

23:17

Resveratrol could be a potential therapeutic target for intrahepatic cholestasis of pregnancy. "IndyWatch Feed Health"

PMID:  Hepatol Res. 2018 Jun 1. Epub 2018 Jun 1. PMID: 29855113 Abstract Title:  Resveratrol Regulating SIRT1-NF-B Signal Pathway in Intrahepatic Cholestasis of Pregnancy. Abstract:  OBJECTIVE: Intrahepatic cholestasis of pregnancy (ICP) is a pregnancy-specific liver disease characterized by raised serum bile acids and adverse fetal outcomes. In this study, we aimed to explore the molecular and biochemical mechanism of resveratrol in regulating SIRT1-NF-B signal pathway and bile acid biosynthesis in intrahepatic cholestasis of pregnancy.METHODS: We analyzed serum and placenta samples from 30 normal and ICP pregnancy women. Then we treated HTR-8/SVneo cells with taurocholic acid (TCA) to mimic ICP condition before dealing these cells with resveratrol as an activator of SIRT1 and EX-57 as an inhibitor of SIRT1. We established ICP rats model to analyze the therapeutic effect of resveratrol.RESULTS: The expression of SIRT1 protein in normal placenta tissues was higher than that in ICP, and the expression of NF-B in normal group was lower than ICP group. SIRT1 was down-regulated, while NF-B and TNF- were up-regulated, in syncytiotrophoblast HTR-8 cells treated by TCA. This phenomenon could be reverted by resveratrol, and these effects could be blocked by Ex-527.CONCLUSION: These data demonstrate that resveratrol may protect syncytiotrophoblast against TCA-induced inflammatory injury by up-regulation of SIRT1 and down-regulation of NF-B and TNF-. Resveratrol could be a potential therapeutic target for intrahepatic cholestasis of pregnancy.

read more

22:42

It is concluded that the cardioprotective effect of resveratrol is due to the antioxidant effect and other antiaging effects. "IndyWatch Feed Health"

PMID:  Oxid Med Cell Longev. 2018 ;2018:1390483. Epub 2018 May 2. PMID: 29854072 Abstract Title:  Evaluation of Oxidative Stress in Cardiomyocytes during the Aging Process in Rats Treated with Resveratrol. Abstract:  The substantial increase in the number of elderly people in our societies represents a challenge for biology and medicine. The societies of the industrialized countries are subject to a progressive aging process that translates into an increase in the cardiovascular risk of the population. In the present work, the activity of catalase and superoxide dismutase was evaluated, as well as markers of oxidative stress (concentration of nitric oxide and total lipoperoxidation in its main components: malondialdehyde and 4-hydroxyalkene) in cardiomyocytes during the aging process in rats treated with resveratrol. Rats were divided into 4 groups according to the following categories: control (without treatment), negative control group (administered with physiological solution with 10% ethanol), positive control group (administered with vitamin E, 2mg/kg/day), and group administered with resveratrol (10mg/kg/day); these groups in turn were divided into 2, 4, 6, and 8 months of treatment. The analysis of nitric oxide showed a decreased level in the cardiac tissue in the groups treated with resveratrol; the same occurs when total lipoperoxidation is analyzed. The enzymatic activity studied (catalase and superoxide dismutase) did not present significant changes with respect to the controls. It is concluded that the cardioprotective effect of resveratrol is due to the antioxidant effect and other antiaging effects and not to the activation of the enzymes catalase and superoxide dismutase.

read more

21:27

Resveratrol sensitizes carfilzomib-induced apoptosis via promoting oxidative stress in multiple myeloma cells. "IndyWatch Feed Health"

PMID:  Front Pharmacol. 2018 ;9:334. Epub 2018 May 14. PMID: 29867453 Abstract Title:  Resveratrol Sensitizes Carfilzomib-Induced Apoptosis via Promoting Oxidative Stress in Multiple Myeloma Cells. Abstract:  The proteasome inhibitor is a target therapy for multiple myeloma (MM) patients, which has increased the overall survival rate of multiple myeloma in clinic. However, relapse and toxicity are major challenges for almost all MM patients. Thus, there is an urgent need for an effective and less toxic combination therapy. Here, we demonstrated that a natural compound, resveratrol (RSV) displayed anti-proliferative activity in a dose- and time-dependent manner in a panel of MM cell lines. More importantly, a low concentration of RSV was synergistic with a low dose of the proteasome inhibitor carfilzomib (CFZ) to induce apoptosis in myeloma cells. Further studies showed that mitochondria was a key regulatory site after RSV/CFZ combination treatment. RSV induced the release of second mitochondria-derived activator of caspase (Smac) in a dose-dependent manner and kept the Smac in a high level after combination with CFZ. Also, RSV was additive with CFZ to increase reactive oxygen species (ROS) production. Moreover, a stress sensor SIRT1, with deacetylase enzyme activity, was remarkably downregulated after RSV/CFZ combination, thereby significantly decreasing its target protein, survivin in MM cells. Simultaneously, autophagy was invoked after RSV/CFZ combination treatment in myeloma cells. Further inhibition of autophagy could increase more ROS production and apoptosis, indicating a close linkage between autophagy and proteasome to modulate the oxidative stress. Together, these findings suggest that induction of multiple stress responses after RSV/CFZ combination is a major mechanism to synergistically inhibit MM cell growth and reduce the toxicity of CFZ in MM cells. This study also provides an important rationale for the clinic to consider an autophagy inhibitor for the combination therapy in MM patients.

read more

21:15

Resveratrol inhibits the proliferation of A549 cells by inhibiting the expression of COX-2. "IndyWatch Feed Health"

PMID:  Onco Targets Ther. 2018 ;11:2981-2989. Epub 2018 May 22. PMID: 29872310 Abstract Title:  Resveratrol inhibits the proliferation of A549 cells by inhibiting the expression of. Abstract:  Purpose: The aim was to investigate resveratrol effects on A549 cells proliferation.Methods: A total of 104 lung adenocarcinoma tissues and nontumor tissues were collected. BEAS-2B cells were cultured in RPMI 1640 medium (group A). A549 cells were treated with RPMI 1640 medium containing different resveratrol concentrations. A549 cells were transfected and grouped as follows: blank group, siRNA-negative control group, siRNA-COX-2 group and resveratrol + siRNA-COX-2 group. qRT-PCR and Western blot were conducted to detectexpression. MTT assay, soft agar clone assay and flow cytometry were performed to assess proliferation and cell cycle.Results: The relative expression ofmRNA was significantly increased in lung adenocarcinoma tissues (

read more

20:55

Resveratrol treatment promoted nerve regeneration and led to rapid motor repair. "IndyWatch Feed Health"

PMID:  Front Neurosci. 2018 ;12:341. Epub 2018 May 23. PMID: 29875625 Abstract Title:  Resveratrol Promotes Nerve Regeneration via Activation of p300 Acetyltransferase-Mediated VEGF Signaling in a Rat Model of Sciatic Nerve Crush Injury. Abstract:  Peripheral nerve injuries are generally associated with incomplete restoration of motor function. The slow rate of nerve regeneration after injury may account for this. Although many benefits of resveratrol have been shown in the nervous system, it is not clear whether resveratrol could promote fast nerve regeneration and motor repair after peripheral nerve injury. This study showed that the motor deficits caused by sciatic nerve crush injury were alleviated by daily systematic resveratrol treatment within 10 days. Resveratrol increased the number of axons in the distal part of the injured nerve, indicating enhanced nerve regeneration. In the affected ventral spinal cord, resveratrol enhanced the expression of several vascular endothelial growth factor family proteins (VEGFs) and increased the phosphorylation of p300 through Akt signaling, indicating activation of p300 acetyltransferase. Inactivation of p300 acetyltransferase reversed the resveratrol-induced expression of VEGFs and motor repair in rats that had undergone sciatic nerve crush injury. The above results indicated that daily systematic resveratrol treatment promoted nerve regeneration and led to rapid motor repair. Resveratrol activated p300 acetyltransferase-mediated VEGF signaling in the affected ventral spinal cord, which may have thus contributed to the acceleration of nerve regeneration and motor repair.

read more

20:34

Resveratrol is a blocker of STAT5 activation and thus may be potentially useful for the treatment of chronic myelogenous leukemia. "IndyWatch Feed Health"

PMID:  Anticancer Drugs. 2018 Jun 6. Epub 2018 Jun 6. PMID: 29878900 Abstract Title:  Resveratrol inhibits STAT5 activation through the induction of SHP-1 and SHP-2 tyrosine phosphatases in chronic myelogenous leukemia cells. Abstract:  STAT5 is an important transcription factor that is constitutively activated in various types of malignancies, including chronic myelogenous leukemia (CML). Whether the antitumor effects of resveratrol (RES) are linked to its capability to inhibit STAT5 activation in CML cells was investigated. We found that RES inhibited STAT5 activation in K562 and KU812 cell lines; RES also reduced the STAT5 concentration in the nucleus of K562 and KU812 cells. Protein tyrosine phosphatase (PTP) inhibitor, sodium pervanadate, reversed the RES-induced downregulation of STAT5, suggesting the involvement of a PTP. Indeed, we observed that RES decreased the expression of tyrosine phosphatase SHP-1 and SHP-2; moreover, the deletion of SHP-1 and SHP-2 genes by siRNA abolished the ability of RES to inhibit STAT5 activation, which suggested the critical role of both SHP-1 and SHP-2 in its possible mechanism of action. RES downregulated the expression of STAT5-regulated gene products such as Bcl-xL, Bcl-2, Cyclin D1, and Mcl-1, and increased the expression of Bax. This correlated with the suppression of proliferation and induction of apoptosis. Overall, our results suggest that RES is a blocker of STAT5 activation and thus may be potentially useful for the treatment of CML.

read more

IndyWatch Health Watch Feed Archiver

Go Back:30 Days | 7 Days | 2 Days | 1 Day

IndyWatch Health Watch Feed Today.

Go Forward:1 Day | 2 Days | 7 Days | 30 Days

IndyWatch Health Watch Feed was generated at Community Resources IndyWatch.

Resource generated at IndyWatch using aliasfeed and rawdog