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by Paul Fassa
Health Impact News
A recent study connecting increased statin drug use with rising ALS was discussed by Dr. Malcom Kendrick. He had followed earlier similar studies with similar concerns, but this one confirmed Dr. Kendricks suspicions.
ALS (Amyotrophic lateral sclerosis), is also known as motor neuron disease (MND), or Lou Gehrigs disease.
It is a progressive neurological disease that destroys neurons in nerves that extend laterally from the spine, thus the word lateral is part of its description. Sclerosis means scarring or hardening, which incapacitates the nerves leading to a loss of muscle control. The term Amyotrophic is a combination of Latin/Greek syllables that means no muscle nourishment leading to complete atrophy.
But the loss of motor control from lateral movement neuron damage progresses to losing the ability to partially or fully physically function in many areas required of the human body, such as walking, talking, eating, walking, and even breathing.
ALS patients can die while slowly suffocating. (Source)
Ones mind normally remains lucid while experiencing ALS. Dr. Kendrick mentions that ALS suffering can be so severe, especially in the late stage of breathing difficulty, that many ALS patients resort to finding places where assisted suicide is allowed to avoid slowly suffocating to death.
The study of concern is Amyotrophic Lateral Sclerosis Associated with Statin Use: A Disproportionality Analysis of the FDAs Adverse Event Reporting System. It was published by the journal Drug Safety in April of 2018.
The researchers at the University of California (San Diego) and Advera Health Analytics, Inc., Santa Rosa, California teamed up to analyze data from the FDA Adverse Event Reporting System (FAERS) to determine what is known as reporting odds ratios...
By Heather Callaghan, Editor
As much as we support supplement freedom in the U.S., there are some very questionable supplements on the market. Some are made from rocks, hair or metals. Iron can build up in the system and become toxic so we are going to have to wave them goodbye. We forget sometimes that iron is a heavy metal, after all. We need a little, not a lot.
According to a new study of cancer cells from Chalmers University of Technology, Sweden, two common iron compounds increase the formation of a known cancer biomarker.
The two compounds, ferric citrate and ferric EDTA, are often used in dietary supplements and as a food additive in the USA and the EU. They are commonly a part of fortified foods, but the types of iron used are rarely listed on supplement bottles or ingredients lists.
Currently, there are many different types of iron supplements on the market. These can be based on at least 20 different iron compounds, and sold under a wide range of brands.
Here, we talk about what iron does in the lab and what you can you do if you need more iron.
Chalmers University reported:
The researchers studied ferric citrate and ferric EDTA, which have both previously been shown to worsen tumour formation in mice with colon cancer. The science behind this has been...
I recently dug up a 1908 curriculum manual in the Minnesota Historical Society archives. It provided instructions on everything from teacher deportment to recommended literature lists for various grades. As a book lover, I was especially interested in the latter!
With the exception of a few textbook-like anthologies, the chart below lists the recommended reading material for Minnesota 7th and 8th graders in 1908:
With such a list in hand, I decided to examine if the common accusation that todays education standards have been dumbed down is really true. To make sure I wasnt unfairly weighting this survey in favor of the past, I went to one of the Twin Cities metro areas finest districts, namely, Edina Public Schools.
Again, with the exception of a few textbook anthologies, the list below offers the reading options for their 7th and...
Comments by Brian Shilhavy
Editor, Health Impact News
Benjamin Krause is a lawyer, investigative reporter, award-winning veterans advocate, and the founder and chief editor of DisabledVeterans.org.
Krause recently published a leaked memo from the Department of the Army, admitting that during a 6-year period, from 2001 through 2007, many soldiers were vaccinated with a bad batch of the anthrax vaccine.
The purpose of the memo was to inform these veterans that they might be entitled to Veteran Affairs (VA) benefits due to the anthrax vaccine.
The memo leaked onto social media and allegedly prompted the Massachusetts Department of Veterans Services to address the memo, calling it a fake memo and a SCAM.
However, Krause reports that the memo was digitally signed and nearly impossible to fake:
A PDF of the memorandum, drafted by a person named Patrick McGrath II was digitally signed using a DOD certificate authority, and that its contents are not subject to repudiation because the certificate chain is intact, according to attorney Francis White, III, a former US Air Force compliance inspector for information technology.
I spoke with White, a colleague of mine in the same office, about his professional impression of the memo and his statement above.
White says faking the signature using a DOD CA certificate chain is nearly impossible in this context, the digital signature on that memo is valid and the related documentation shows it was signed by an individual named Patrick Steven McGrath II, and is not subject to repudiation
White says that does not mean McGrath had the authority to circulate the memo to the public, but it d...
PMID: Food Funct. 2018 Mar 28. Epub 2018 Mar 28. PMID: 29589631 Abstract Title: Broad bean (Vicia faba L.) pods: a rich source of bioactive ingredients with antimicrobial, antioxidant, enzyme inhibitory, anti-diabetic and health-promoting properties. Abstract: This study was aimed at investigating the chemical composition (proximate, minerals, fatty acids and phenolic compounds) and the in vitro (antimicrobial, radical scavenging, anti-acetylcholinesterase and protein denaturing activities) and in vivo (anti-diabetic and histo-protective effects in alloxan-induced diabetic mice) biological activities of broad bean pods (BBPs), a food waste by-product material. The results showed that BBPs have high dietary fiber (57.46%), carbohydrate (18.93%) and protein (13.81%) content versus low fat content (
The reaction mechanism for converting hydrogen fluoride (HF)
impurity from the electrolyte into lithium fluoride (LiF) in the
solid-electrolyte interphase (SEI) with release of hydrogen gas
(H2). The SEI layer is shown on a substrate of gold (Au) atoms,
which serves as a simplified model system. Scientists determined
this mechanism using advanced computational methods (density
functional theory and molecular dynamics simulations). Credit:
Argonne National Laboratory>
Commercially available since the 1970s, the lithium-ion battery is now the workhorse power source in many applications. It can be found in cell phones, laptops and electric vehicles. Yet, much about the basic science taking place at the atomic and molecular levels during charge and discharge remains a mystery.
In a new study published in Nature Catalysis, a team at the U.S. Department of Energys (DOE) Argonne National Laboratory reports a breakthrough in understanding the chemistry of the microscopically thin layer that forms at the interface between the liquid electrolyte and solid electrode. Battery researchers commonly refer to this layer as the solid-electrolyte interphase or SEI.
Much scientific work over the last several decades has been devoted to understanding the SEI in the lithium-ion battery. Scientists know that the SEI forms on the graphite negative electrode, is extremely thin (less than a thousandth of a millimeter), and primarily takes shape during the first charge of the battery. Also well-established is that the SEI prevents detrimental reactions from occurring at the interface, while at the same time allowing the important lithium ions free rein to move between the electrolyte and electrode.
All good lithium-ion batteri...
Stephanie Mencimer is a 47-year-old writer. By all her accounts, she had taken the necessary steps to be free and clear of breast cancer. Careful diet, exercise, BPA free water bottles and breastfeeding her children were all part of Stephanies regimen. Additionally, Stephanie was a decade and a half younger than the median age for... View Article
The post Drinking May Have Given Me Cancer. The Alcohol Industry Has Worked Hard to Bury the Link appeared first on Healthy Holistic Living.
PMID: Plast Reconstr Surg. 2018 Mar 30. Epub 2018 Mar 30. PMID: 29649056 Abstract Title: Topical Aloe Vera Gel for Accelerated Wound Healing of Split-Thickness Skin Graft Donor Sites: A Double-Blind, Randomized, Controlled Trial and Systematic Review." Abstract: BACKGROUND: Aloe vera has been used to treat wounds since ancient times. However, the efficacy of Aloe vera for burns and split-thickness skin graft (STSG) donor sites are inconclusive.METHODS: A double-blind, randomized, controlled trial was conducted. Patients who underwent STSG harvesting from the thigh were included. STSG donor sites were divided into two groups: Aloe vera group and placebo group. The visual analogue scale (VAS) score evaluated pain and complete epithelialization was assessed. We searched electronic databases and included only international clinical trials published in the English language.RESULTS: Twelve patients with 24 donor sites participated. Times to complete epithelization for the Aloe vera and placebo groups were 11.5 + 1.45 and 13.67 + 1.61 days (p
PMID: Clujul Med. 2017 ;90(3):344-347. Epub 2017 Jul 15. PMID: 28781531 Abstract Title: Antibacterial and antifungal activity of endodontic intracanal medications. Abstract: BACKGROUND AND AIMS: The sterilization of the entire root canal system represents the main goal of every endodontist, given the fact that the control of the microbial flora is the key point of every root canal treatment. The diversity of microorganisms found inside the root canal and also the resistance of some bacterial species to intracanal medications led to a continuous development of new endodontic products. The present study focuses on the comparison of the antibacterial and antifungal properties of different endodontic products, two commercially available, one experimental plant based extract, and two control substances.METHODS: The disc diffusion assay was used to determine the antibacterial and antifungal properties of chlorhexidine, calcium hydroxide, a mix extract between Arctium lappa root powder and Aloe barbadensis Miller gel, Amoxicillin with clavulanic acid and Fluconazole (as control substances). Two of the most common microorganisms found in endodontic infections were chosen: Enterococcus faecalis (ATCC 29212) and Candida albicans ATCC(10231).RESULTS: All tested substances showed inhibition zones around the discs, for Enterococcus faecalis and Candida albicans, including the experimental mix extract of Arctium lappa root powder with Aloe vera gel.CONCLUSION: The experimental mix extract of Arctium lappa root powder and Aloe vera gel is able to inhibit very resistant microorganisms, like Enterococcus faecalis and Candida albicans.
PMID: Phytother Res. 2018 Apr ;32(4):631-642. Epub 2017 Dec 13. PMID: 29235180 Abstract Title: The effects of hypoglycemia and weight loss of total lignans from Fructus Arctii in KKAy mice and its mechanisms of the activity. Abstract: Fructus Arctii (great burdock achene) is the dried ripe fruit of Arctium lappa L. (family Asteraceae) and is included in the Chinese pharmacopoeia. It has been reported that the clinical use of Fructus Arctii resulted in a satisfactory hypoglycemic effect in diabetic patients. This study aimed to investigate antidiabetic activity and mechanism of total lignans from Fructus Arctii (TLFA) in KKAy mice, a spontaneous Type 2 diabetic rodent model that exhibits marked obesity. In this study, KKAy mice were gavaged once daily with solvents (0.5% sodium carboxymethyl cellulose), TLFA (250 and 125 mg/kg), or metformin (200 mg/kg) for 11 weeks, and C57BL/6J mice treated with saline solution (0.9%, w/v) were used as normal control. The results indicate that TLFA has dual effects of hypoglycemia and weight loss, and administration of TLFA in KKAy mice could decrease fasting blood glucose, glycated hemoglobin, and body weight; improve oral glucose tolerance; increase high density lipoprotein cholesterol; and decrease triglycerides and free fatty acid in mice serum. Its efficacy may associate with multiple mechanisms of action such as stimulation of insulin secretion, activation of phosphatidylinositol 3-kinase/protein kinase B, and adenosine-monophosphate-activated protein kinase signaling pathway, decreasing leptin.
NEW BEDFORD This spring, rainwater collection barrels offered by The Great American Rain Barrel Company will be available for residents of New Bedford.
Harvesting rainwater with a rain barrel can mitigate the irregular patterns of rainfall. To help homeowners and the City manage their water supplies and encourage water conservation, New Bedford is offering residents the opportunity to purchase rain barrels at a discount.
Saving rainwater helps homeowners hedge against the cost of water. Rainwater is a free healthy alternative; it is organic, has no chloramine, no fluoride and no chemicals. It is an excellent water source for plants, gardens, lawns and any non-potable use.
Regular use of a rain barrel can pay for itself in one season. One tenth of an inch of rain on a 1000-square-foot roof about 25 minutes of moderate rainfall will fill a 60-gallon rain barrel. New England typically receives 17 inches of rain during the growing season from May through September. This provides enough water to fill 170 rain barrels. At 2.78 cents per gallon, homeowners can expect to save about $280 worth of water (based on current New Bedford water rates).
Droughts stress residential landscaping and town water facilities; in 2016, 168 communities in Massachusetts faced restrictions on nonessential outdoor water use a full 47% of the cities and towns in the state. There is no restriction on rainwater use.
The Great American Rain Barrel Co., a local food importing company, has been repurposing shipping drums into Rain Barrels since 1988. Last year, 30 percent of Massachusetts cities and towns partnered with The Great American Rain Barrel Co, selling over 3000 barrels. The Great American Rain Barrel Co. has been approved as vendors by the Massachusetts Department of Environmental Protection (DEP) for the state since 2010.
Barrels are being offered to residents for $69 a savings of 40 percent off the retail price. To learn more about the barrels, and to order a barrel, please visit: www.greatamericanrainbarrel.com/community and select New Bedford. Residents may also order a barrel by calling 1-800-251-2352. The deadline to order barrels is Sunday, April 29.
Once ordered, the barrels will be available for pick up on Saturday, May 5 at the New Bedford Department of Public Infrastructures office at 1103 Shawmut Avenue in New Bedford from 9 a.m. to 11 a.m.
About The Great American Rain Barrel Co.:
100% re-purposed rain barrels produced in Massachusetts.
PMID: J Pharmacol Sci. 2017 Nov ;135(3):97-104. Epub 2017 Aug 10. PMID: 29108833 Abstract Title: Arctiin protects against cardiac hypertrophy through inhibiting MAPKs and AKT signaling pathways. Abstract: PURPOSE: The mitogen-activated protein kinases (MAPKs) and protein kinase B (AKT) pathways have emerged as essential intracellular signaling pathways in eukaryotic cells, particularly as regulators of cardiac hypertrophy. Previous studies indicated that arctiin, an active ingredient of biennial dried ripe burdock, could exhibit potent anti-inflammatory and anti-allergic activities via down-regulating the activation of MAPKs and AKT pathways. However, little is known about its effects on cardiac hypertrophy. Therefore, the present study aimed to explore whether arctiin could attenuate cardiac hypertrophy.GENERAL METHODS: Arctiin (80 mg/kg) was administered by oral gavage once daily for 3 weeks from 1 week after surgery. Then, the mice were subjected to either chronic pressure overload generated by aortic banding (AB) or sham surgery (control group). Cardiac function was assessed by echocardiography.FINDINGS: The results indicated that arctiin attenuated cardiac hypertrophy induced by AB, and suppressed cardiac fibrosis and accumulation of collagen in vivo. Arctiin also inhibit the activation of MAPKs and AKT occurs in response to hypertrophic stimuli. Arctiin attenuated phenylephrine-induced hypertrophy of myocytes in vitro.CONCLUSIONS: In conclusion, arctiin can improve cardiac function and prevent the development of cardiac hypertrophy by blocking the MAPKs and AKT signaling pathways.
PMID: Mol Med Rep. 2017 Dec ;16(6):9120-9124. Epub 2017 Oct 2. PMID: 28990056 Abstract Title: Arctiin regulates collagen type 1 chain 1 mRNA expression in human dermal fibroblasts via the miR378bSIRT6 axis. Abstract: Arctiin, a lignin isolated from Arctium lappa, exhibits a variety of biological effects, including antiviral, antiinflammatory, and antiproliferative actions, in mammalian cells. In a previous study, arctiin was demonstrated to induce procollagen type I synthesis and exhibited protective effects against ultraviolet B (UVB) radiation in normal human dermal fibroblasts (nHDFs). However, the underlying molecular mechanism of arctiinmediated collagen synthesis remains unknown. In the present study, the mechanism for increased expression of collagen type 1 1 chain (COL1A1) mRNA in arctiininduced nHDFs was identified. The expression of microRNA378b (miR378b), downregulated by arctiin, was correlated with the expression of sirtuin6 (SIRT6) mRNA, a regulator of COL1A1 mRNA. Furthermore, it was revealed that arctiin protected the UVB radiationmediated decrease in COL1A1 mRNA expression, through the miR378b/SIRT6 signaling pathway. In conclusion, these results suggest that arctiin regulates COL1A1 through the miR378bSIRT6 axis.
Spring has sprung! Tis the season for rebirth and regeneration. Spring is ruled by the wood element which is associated with the liver and its complementary organ, the gallbladder. What better time to reset and rejuvenate body, mind, and spirit than through the liver, an organ known to regenerate itself if a portion is surgically removed?
It was at one time considered the seat of life; hence its name liver, the thing we live with. -Ambrose Bierce, The Devils Dictionary, Tales and Memoirs
As the bodys largest filter and gland, the liver is a detox powerhouse responsible for more than 500 (possibly 5000) functions. Your liver filters 1 liter of blood every minute from the digestive tract before it flows to the rest of the body. It holds about one pint of blood (13% of the bodys blood) at any given moment. It detoxifies, nourishes, replenishes, and stores blood. It also energizes the blood by releasing stored sugar (glycogen) as glucose. Other jobs of your liver include:
The liver is also...
There are three kinds of lies: lies, damned lies, and statistics. Mark Twain
most flu appears to have nothing to do with influenza. Every year, hundreds of thousands of respiratory specimens are tested across the US. Of those tested, on average 16% are found to be influenza positive. From the British Medical Journal editor, Peter Doshi, MD
PMID: J Vet Med Sci. 2017 Oct 7 ;79(10):1698-1706. Epub 2017 Sep 11. PMID: 28890521 Abstract Title: Effect of burdock extract on physical performance and physiological fatigue in mice. Abstract: : Burdock (BD) is a common vegetable with many pharmacological properties. However, few studies have examined the effect of BD on exercise performance and physical fatigue. We aimed to evaluate the potential beneficial effects of BD on fatigue and ergogenic functions following physical challenge in mice.METHODS: Male ICR mice were divided into four groups to receive either vehicle, or BD at 348.5, 697 or 1,742.5 mg/kg/day, by daily oral gavage for 4 weeks. Exercise performance and fatigue were evaluated from forelimb grip strength, exhaustive swimming time, and post-exercise levels of physical fatigue-related biomarkers serum lactate, ammonia, glucose, and creatine kinase (CK).RESULTS: BD supplementation elevated endurance and grip strength in a dose-dependent manner. It also significantly decreased lactate, ammonia, and CK levels after physical challenge. In addition, BD supplementation had few subchronic toxic effects.CONCLUSIONS: Supplementation with BD has a wide spectrum of bioactive effects, including health promotion, performance improvement, and fatigue reduction.
PMID: Nutr Cancer. 2018 Jan ;70(1):125-135. Epub 2017 Nov 17. PMID: 29148840 Abstract Title: Resveratrol Induces Mitochondrial Apoptosis and Inhibits Epithelial-Mesenchymal Transition in Oral Squamous Cell Carcinoma Cells. Abstract: OSCC is the most common malignant cancer of the head and neck. EMT is an essential cellular process critical to the morphogenesis and homeostasis of solid tissues. It is also involved in the initial stage of cancer metastasis and invasion in which cells lose epithelial characteristics. While cancer therapy protocols such as surgery, radiation, and chemotherapy are effective and useful, the drug tolerance and toxicity of OSCC patients remain a problem. Resveratrol is mainly produced in red grape skin and exhibits anti-oxidative, anti-inflammatory, anti-proliferative, and anti-cancer properties. This study was undertaken to investigate the underlying mechanisms giving rise to the induction of apoptosis by resveratrol in the human tongue squamous cell carcinoma cell line. Resveratrol treatment resulted in a time- and dose-dependent decrease in cell viability and increased the apoptotic cell ratio in CAL-27, SCC15, and SCC25 cells. Resveratrol treatment of CAL-27 cells showed that several lines of apoptotic manifestation and decreased cell migration, invasion, and EMT-inducing transcription factor. Taken together, our findings demonstrate the inhibitory effect of resveratrol in human OSCC cells via the mitochondrial pathway and that resveratrol is able to inhibit cell invasion and migration by inhibiting the EMT-inducing transcription factors.
PMID: Proc Natl Acad Sci U S A. 2018 Feb 6 ;115(6):E1239-E1248. Epub 2018 Jan 24. PMID: 29367423 Abstract Title: Chemotherapy induces enrichment of CD47/CD73/PDL1immune evasive triple-negative breast cancer cells. Abstract: Triple-negative breast cancer (TNBC) is treated with cytotoxic chemotherapy and is often characterized by early relapse and metastasis. To form a secondary (recurrent and/or metastatic) tumor, a breast cancer cell must evade the innate and adaptive immune systems. CD47 enables cancer cells to evade killing by macrophages, whereas CD73 and PDL1 mediate independent mechanisms of evasion of cytotoxic T lymphocytes. Here, we report that treatment of human or murine TNBC cells with carboplatin, doxorubicin, gemcitabine, or paclitaxel induces the coordinate transcriptional induction of CD47, CD73, and PDL1 mRNA and protein expression, leading to a marked increase in the percentage of CD47CD73PDL1breast cancer cells. Genetic or pharmacological inhibition of hypoxia-inducible factors (HIFs) blocked chemotherapy-induced enrichment of CD47CD73PDL1TNBC cells, which were also enriched in the absence of chemotherapy by incubation under hypoxic conditions, leading to T cell anergy or death. Treatment of mice with cytotoxic chemotherapy markedly increased the intratumoral ratio of regulatory/effector T cells, an effect that was abrogated by HIF inhibition. Our results delineate an HIF-dependent transcriptional mechanism contributing to TNBC progression and suggest that combining chemotherapy with an HIF inhibitor may prevent countertherapeutic induction of proteins that mediate evasion of innate and adaptive antitumor immunity.
World Mercury Project (WMP) Note: This important article exposes how Goldman-Sachs looks at the business model of cure vs. no cure for diseases. The report asks the question: Is curing patients a sustainable business model? WMP is working to change this model. By the World Mercury Project Team Together, the U.S. government and American families are spending billions on medications, therapy, insurance,
You all know me: I love good, healthy, whole foods. LOVE. So I was absolutely thrilled when the team at the Food Revolution Summit asked me to invite you all to join 300,000+ other healthy food lovers for this totally FREE online event, beginning April 28th and going thru May 6th. When you sign up, youll get the latest cutting-edge insights
Members of the Houston City Council heard input Monday during a hearing and later at their regular meeting on fluoridation.
The six-member council could decide to continue the 16-year-old practice, opt to end it or place the issue on a ballot. Two years ago, the council decided not to end it.
The most recent debate brought two national organizations to the Texas County seat one against and another that supports it. Both sides spoke about 10 minutes each at the council meeting.
Dr. Paul Connett, a retired professor of chemistry and current director of the Fluoride Action Network, said the practice, which extends over generations, is unsafe, that children are being overexposed and forcing people to swallow fluoride made no sense. Additionally, he said damage can occur to teeth, bones and the brain, which is supported by study. He urged the council to support a more concentrated effort on education to fight tooth decay, obesity and chronic diseases, such as diabetes.
Johnny Johnson Jr., president of the American Fluoridation Society, disputed Connetts assertions and studies cited. He said across the country, adding fluoride had greatly reduced cavities and introduced studies he said were conducted at the highest standard including a finding just released that found no damage with even higher doses.
Johnson said public hearings have played out across the country over years, calling it a waste of time and energy, adding it is safe, effective and not hurting anyone.
The council meets next May 7.
PMID: Bull Cancer. 2014 Sep ;101(9):876-80. PMID: 25296414 Abstract Title: [Radiation-induces increased tumor cell aggressiveness of tumors of the glioblastomas?]. Abstract: Glioblastoma multiform is the most common and aggressive brain tumor with a worse prognostic. Ionizing radiation is a cornerstone in the treatment of glioblastome with chemo-radiation association being the actual standard. As a paradoxal effect, it has been suggested that radiotherapy could have a deleterious effect on local recurrence of cancer. In vivo studies have studied the effect of radiotherapy on biological modification and pathogenous effect of cancer cells. It seems that ionizing radiations with photon could activate oncogenic pathways in glioblastoma cell lines. We realized a review of the literature of photon-enhanced effect on invasion and migration of glioblastoma cells by radiotherapy.
It had all the trappings of a debate on a national stage: The countrys foremost critic of fluoridation and representatives of the national organization that promotes its use with a local medical community that has seen its benefits.
And it all happened in Houston, Mo., on a Monday night with the Houston City Council looking on as it found itself reliving a decision made 16 years ago to add fluoride to the water supply to improve dental health in the community. Houston is among about 75 percent of the countrys population now covered. Cabool also is included after generations of Cartwrights the towns early dentists promoted it. Supporters say the benefits are undeniable. Critics say it is a health danger.
Houstons city government says the discussion was sparked by questions raised by George Sholtz, an Upton resident and a principal in a radio station whose airwaves cover Texas County and other regions. Sholtz, who frequently attends council meetings, raised questions about fluoridation at an earlier meeting and in writing. The council called for a public hearing and asked questions.
What resulted was a visit organized by opponents, including Sholtz and Marie Lasater of Licking, from Dr. Paul Connett, who operates an anti-fluoridation group, Fluoride Action Network. That led the president of the American Fluoridation Society, Dr. Jonny Johnson Jr., and a colleague, Steve Slott, to arrive to provide an alternative view in an exchange at the Houston Storm Shelter and later at city hall, the venue for that early hearing in 2002 that filled the towns city hall.
The one-hour hearing allowed anyone to give a three-minute comment to the council. Later, the principals in the debate each had time to address the council at Houston City Hall during its regular meeting.
Heres what was said at the shelter with City Administrator Tona Bowen at the time clock as the speakers rose for three minutes:
Members of the Hous...
U.K. company GW Pharmaceuticals Plc has created the first drug derived from the marijuana plant that might soon be approved in the U.S. Created with plants bred to have high cannabidiol, or CBD, content, the medication is a purified chemical made into a strawberry-flavored oral solution. GW Pharma hopes to market the medication under the name Epidiolex. The
PMID: J Agric Food Chem. 2017 Nov 1 ;65(43):9443-9452. Epub 2017 Oct 17. PMID: 28976750 Abstract Title: Inhibition of Mast Cell-Mediated Allergic Responses by Arctii Fructus Extracts and Its Main Compound Arctigenin. Abstract: The Arctium lappa seeds (Arctii Fructus) and its major active compound, arctigenin (ARC), are known to have anticancer, antiobesity, antiosteoporosis, and anti-inflammatory activities. However, the effect of Arctii Fructus and ARC on mast cell-mediated allergic inflammation and its associated mechanism have not been elucidated. Therefore, we attempted to investigate the antiallergic activity of Arctii Fructus and ARC on mast cells and experimental mouse models. Arctii Fructus water extract (AFW) or ethanol extract (AFE) and ARC reduced the production of histamine and pro-inflammatory cytokines such as interleukin (IL)-1, IL-6, IL-8, and TNF- in mast cells. AFW, AFE, and ARC inhibited phosphorylation of MAPKs and NF-B in activated mast cells. Moreover, IgE-mediated passive cutaneous anaphylaxis and compound 48/80-induced anaphylactic shock were suppressed by AFW, AFE, and ARC administration. Theseresults suggest that Arctii Fructus and ARC are potential therapeutic agents against allergic inflammatory diseases.
PMID: Sci Rep. 2018 Feb 19 ;8(1):3307. Epub 2018 Feb 19. PMID: 29459629 Abstract Title: Experimental study of the anti-tumour activity and pharmacokinetics of arctigenin and its valine ester derivative. Abstract: Arctigenin (ARG) is a functional active component that has important physiological and pharmacological activities. The anti-tumour and anti-inflammatory activities of ARG show good potential for application and development, but this material has the defect of low water solubility. In this experiment, the valine derivative of ARG (ARG-V) was designed and synthesized to overcome this disadvantage. The ARG amino acid, EDCI and DMAP were raw materials in the addition reaction, with a molar ratio of 1:2:2:0.5. The yield of ARG-V was up to 80%. ARG-V has strong anti-tumour activity in vivo and in vitro. The inhibitory rate of ARG-V was 69.2%, with less damage to the immune organs and different degrees of increased serum cytotoxicity. Moreover, the pharmacokinetics of ARG following oral administration and ARG-V following oral administration in rats were also studied. The Cand AUC values of ARG-V showed significant differences compared to ARG. The relative bioavailabilities of three doses of ARG-V compared to ARG were 664.7%, 741.5% and 812.9%. These pharmacokinetic results may be useful for further studies of the bioactive mechanism of ARG and provide a theoretical basic for clinical use.
The thought of a cupcake, skillfully frosted with fluffy vanilla icing, may put a smile on your face, but research suggests that, in the long term, a sweet tooth may turn that smile into a frown but not for the reasons you think. In a new study, published in Scientific Reports, my colleagues and I found a link between a diet high in sugar and common mental disorders.
The World Health Organisation recommends that people reduce their daily intake of added sugars (that is, all sugar, excluding the sugar that is naturally found in fruit, vegetables and milk) to less than 5% of their total energy intake. However, people in the UK consume double in the US, triple that amount of sugar. Three-quarters of these added sugars come from sweet food and beverages, such as cakes and soft drinks. The rest come from other processed foods, such as ketchup.
At the same time, one in six people worldwide suffers from a common mental disorder, such as a mood or anxiety disorder. Could there be a link between high sugar consumption and common mental disorders?
Earlier research, published in 2002, exa...
An accidental discovery is showing promise in our fight against the plastic that is taking over and polluting our world. In 2016, a bacterium that had naturally evolved to eat plastic was discovered at a dump in Japan and scientists have now revealed the detailed structure of the crucial enzyme produced by the bug. The international
Title: Oil producer Afren fires CEO, 3 other executives in scandal What Caught Our Attention: Two days ago, the news organization Reuters mysteriously withdrew a 2014 story about the firing of four people from now-defunct oil company Afren, including its CEO. The only explanation: it did not meet Reuters standards for accuracy. The executives were Continue reading Caught Our Notice: Why did Reuters remove a 3.5-year-old story?
PMID: Front Pharmacol. 2018 ;9:268. Epub 2018 Mar 27. PMID: 29636686 Abstract Title: Arctigenin Inhibits Liver Cancer Tumorigenesis by Inhibiting Gankyrin Expression via C/EBP and PPAR. Abstract: Burdock () is a popular vegetable in China and Japan that is consumed for its general health benefits. The principal active component of burdock is arctigenin, which shows a range of bioactivitiesand. Here, we investigated the potential anti-tumor effects of arctigenin using two human hepatocellular carcinoma (HCC) cell lines, HepG2 and Hep3B, and sought to elucidate its potential mechanisms of action. Our results showed that arctigenin treatment inhibited cell growth in both HepG2 and Hep3B cell lines (ICof 4.74 nM for HepG2 cells, and of 59.27 nM for Hep3B cells). In addition, migration, invasion, and colony formation by HepG2 cells were significantly inhibited by arctigenin. By contrast, treatment of Hep3B cells with arctigenin did not alter these parameters. Arctigenin also significantly reduced the levels of gankyrin mRNA and protein in HepG2 cells, but not in Hep3B cells. A luciferase assay indicated that arctigenin targeted the -450 to -400 region of the gankyrin promoter. This region is also the potential binding site for both C/EBP and PPAR, as predicted and confirmed by an online software analysis and ChIP assay. Additionally, a co-immunoprecipitation (Co-IP) assay showed that binding between C/EBP and PPAR was increased in the presence of arctigenin. However, arctigenin did not increase the expression of C/EBP orPPAR protein. A binding screening assay and liquid chromatography-mass spectrometry (LC-MS) were performed to identify the mechanisms by which arctigenin regulates gankyrin expression. The results suggested that arctigenin could directly increase C/EBP binding to the gankyrin promoter (-432 to -422 region), but did not affect PPAR binding. Expression of gankyrin,, andwere analyzed in tumor tissues of patients using real-time PCR. Bothandshowed negative correlations with gankyrin. In tumor-bearing mice, arctigenin had a significant inhibitory effect on HCC growth. In conclusion, our results suggested that arctigenin could inhibit liver cancer growth by directly recruiting C/EBP to the gankyrin promoter. PPAR subsequently bound to C/EBP, and both had a negative regulatory effect on gankyrin expression. This study has identified a new mechanism of action of arctigenin against liver cancer growth.
Perth, Australian moms-to-be are needed for a world-first vaccine trial, aiming to protect their unborn babies from a deadly virus. In other words, the vaccine industry is recruiting pregnant women to participate in vaccine experiments....
The post Vaccine companies recruiting pregnant women for experiments appeared first on Vaxxter.
By Dr. Mercola Google and Facebook are two of the largest and clearest monopolies in the world, and between them, the harvesting of your personal information goes far beyond what most people realize was even possible. I have recently removed Google Search from this website and replaced with a search engine without the privacy concerns,
PMID: Am J Chin Med. 2000 ;28(2):163-73. PMID: 10999435 Abstract Title: Hepatoprotective effects of Arctium lappa on carbon tetrachloride- and acetaminophen-induced liver damage. Abstract: The root of Arctium lappa Linne (A. lappa) (Compositae), a perennial herb, has been cultivated for a long time as a popular vegetable. In order to investigate the hepatoprotective effects of A. lappa, male ICR mice were injected with carbon tetrachloride (CCl4, 32 microl/kg, i.p.) or acetaminophen (600 mg/kg, i.p.). A. lappa suppressed the SGOT and SGPT elevations induced by CCl4 or acetaminophen in a dose-dependent manner and alleviated the severity of liver damage based on histopathological observations. In an attempt to elucidate the possible mechanism(s) of this hepatoprotective effect, glutathione (GSH), cytochrome P-450 (P-450) and malondialdehyde (MDA) contents were studied. A. lappa reversed the decrease in GSH and P-450 induced by CCl4 and acetaminophen. It was also found that A. lappa decreased the malondialdehyde (MDA) content in CCl4 or acetaminophen-intoxicated mice. From these results, it was suggested that A. lappa could protect the liver cells from CCl4 or acetaminophen-induced liver damages, perhaps by its antioxidative effect on hepatocytes, hence eliminating the deleterious effects of toxic metabolites from CCl4 or acetaminophen.
PMID: Int J Obstet Anesth. 2018 Feb 16. Epub 2018 Feb 16. PMID: 29544720 Abstract Title: Pruritus after intrathecal morphine for cesarean delivery: incidence, severity and its relation to serum serotonin level. Abstract: BACKGROUND: Pruritus is the most common side effect of intrathecal morphine, especially in parturients. The exact mechanism is not clear and many possible mechanisms have been suggested. Among these is the activation of the 5-hydroxytryptamine sub-type-3 receptors by intrathecal morphine.METHODS: Forty parturients who underwent elective cesarean delivery under spinal anesthesia were divided into two groups of 20 each in this prospective, randomized study. Both groups received an intrathecal injection of 0.5% (2-3mL) hyperbaric bupivacaine in addition to 100g of morphine in group 1 (M100) and 200g of morphine in group 2 (M200). Two blood samples were taken from each patient for serotonin estimation, preoperatively and four hours later. Postoperatively, all patients were assessed for pruritus (incidence and severity), pain (visual analog pain scale), first request for analgesia, and total analgesic dose required within 24hours.RESULTS: The serum serotonin level increased significantly postoperatively, by 283% versus 556% (P
By Gary G. Kohls, MD, guest to Natural Blaze
There are three kinds of lies: lies, damned lies, and statistics. Mark Twain
most flu appears to have nothing to do with influenza. Every year, hundreds of thousands of respiratory specimens are tested across the US. Of those tested, on average 16% are found to be influenza positive. From the British Medical Journal editor, Peter Doshi, MD
71 people would need to be treated with the flu vaccine to prevent one case. In other words, the flu vaccine did nothing for 70 out of 71 who received it. That means this study found the flu vaccine failed 99% (70/71). Dr David Brownstein
Under ideal conditions (when the vaccine actually matches the main viruses circulating that season) you need to vaccinate 33 healthy adults to avoid one set of influenza symptoms. 33 is what is called the NNV (Number Needed to Vaccinate). Dr Tom Jefferson of the Cochrane Collaboration (Cochrane Database Syst. Rev. 2018. Feb. 1,2:CD001269)
influenza virus often mutates to adapt to being grown in chicken eggs, which can influence antigenicity and hence vaccine effectiveness. Ian A. Wilson, MD, et al...
PMID: Anesth Analg. 2018 Mar 27. Epub 2018 Mar 27. PMID: 29596097 Abstract Title: Repeated Morphine Prolongs Postoperative Pain in Male Rats. Abstract: BACKGROUND: Opioids are effective postoperative analgesics. Disturbingly, we have previously reported that opioids such as morphine can worsen inflammatory pain and peripheral and central neuropathic pain. These deleterious effects are mediated by immune mediators that promote neuronal hyperexcitability in the spinal dorsal horn. Herein, we tested whether perioperative morphine could similarly prolong postoperative pain in male rats.METHODS: Rats were treated with morphine for 7 days, beginning immediately after laparotomy, while the morphine was tapered in a second group. Expression of genes for inflammatory mediators was quantified in the spinal dorsal horn. In the final experiment, morphine was administered before laparotomy for 7 days.RESULTS: We found that morphine treatment after laparotomy extended postoperative pain by more than 3 weeks (time treatment: P
I am a professor and head of the clinical neuropsychology working group in the Department of Psychiatry and Psychotherapy at the University Medical Center in Hamburg, Germany. In 2002, our group developed a metacognitive training program (meta = Greek for above/beyond, cognition = derived from Latin for thinking; thinking about thinking). The training can be downloaded at no cost via www.uke.de/mct and is available in 33 languages (for a review see Moritz et al., 20141). For me it is very important that as researchers we serve the public and should not take money for our inventions.
MCT is based on the theoretical foundations of the cognitive-behavioral model of psychosis, but it employs a somewhat different therapeutic approach. The program is comprised of eight modules targeting common cognitive biases (i.e., distortions in the processing of information) in psychosis/schizophrenia. These errors and biases, on their own or in combination, may culminate in the formation of false beliefs to the point of delusions (i.e., fixed false beliefs; Garety & Freeman, 20132; Savulich, Shergill, & Yiend, 20123). The sessions aim to raise participants awareness of these distortions and to prompt them to critically reflect on, expand upon, and change their current repertoire of problem solving. Since psychosis does not occur instantaneously and suddenly, but is often preceded by a gradual change in the appraisal of ones cognitions and social environment (e.g., Klosterktter, 19924), empowering metacognitive competence may act prophylactically to prevent or hinder a psychotic breakdown.
Each module starts with psychoeducational elements and normalizing of symptoms. Through presentation of many examples and exercises, each respective MCT topic is introduced (e.g., jumping to conclusions) and the fallibility of human cognition in general is discussed and illustrated. With regard to normalizing, we stress that cognitive biases and errors are normal to some degree. To illustrate, according to a study, many Americans believe that they saw Bugs Bunny while visiting Disney Land. This is extremely unlikely, however, as it is a Warner Brothers character and anyone with such a costume would likely be asked to leave by guards within minutes.
By Kent Heckenlively, JD I say this in jest, but I believe my logic is sound. You decide. Would you agree with me that any country which interferes in our democracy should fear not just economic sanctions, but military action as well? Okay, talk among yourselves for a minute. It looks like we have a Continue reading Should Trump Launch Missile Strikes on England and Australia?
The post Should Trump Launch Missile Strikes on England and Australia? appeared first on BolenReport.
Researchers from Penn States college of medicine and Yales school of medicine have discovered that the timing of when vaccines are given may be related to major brain disorders. Using insurance data for children ages 6-15,...
The post Penn State Study Shows Correlation Between Vaccines and Brain Disorders appeared first on Vaxxter.
In the video below, Dr. Greger discusses what the science says about the role diet plays in our brain health. This quick video is a must for anyone who is aging or dealing with a relative in the beginning stages of dementia or Alzheimers Disease. RELATED STORY: This vegetable puts the brakes on beta-amyloid and Alzheimers
Does incorporating gardens and their harvest into school-based nutrition programs help children get healthier? A 2017 paper claims it does, but a group of outside experts disagrees strongly. The 2017 paper reported that adding gardens to schools and teaching kids how to cook the harvest, among other elements, helped kids learn about nutrition Continue reading Nutrition paper claims intervention cuts child obesity. Experts disagree.
In Europe, every 90 seconds occurs a death due to heart conditions, yearly are being spent over 18 Billion with cardiovascular diseases. In USA are spent over 70 Billion p.a. In USA, there are over 600,000 people dying yearly because of heart conditions and one quarter of them are younger than 65. Half of people
Salt is the most common and readily available nonmetallic mineral in the world. Yet some people are far more susceptible to the effects of salt than others and scientists are beginning to understand why. Why does a low salt diet increase the risk of death for some and not others?
In this episode of WBURs On Point Radio, Benedict Carey, author of the recent New York Times report on antidepressant withdrawal, discusses the topic of discontinuing antidepressants alongside psychiatrists Eliza Menninger and Ronald Pies.
Photo Credit: "Reading politics," by Grant (Flickr)
By Dr. Mercola
According to the National Coffee Association1 more Americans drank coffee in 2017 than they had in the past four years. This represents a reversal as gourmet coffee brews gain popularity, especially among younger consumers. In an online survey, National Coffee Drinking Trends2 reported coffee drinking was up from 57 percent in 2016, reaching 62 percent of the general population in 2017, with the most pronounced increase in gourmet coffees.
The beverage of choice has been coffee for a number of years, far outpacing other beverages, including bottled water, soda and tea by nearly 50 percent.3 Dark roasted coffee has specific health benefits related to its high levels of antioxidants. For instance, several studies have found lower mortality rates in individuals who drink coffee compared to those who don't drink coffee at all.4,5 Another found those who drink moderate amounts were less likely to have calcium deposits in their coronary arteries.6
The likely culprit behind problems drinking a cup of joe rich in phenols and antioxidants stems from the creamers and sugar added, the pesticides sprayed on the crops, or improper roasting, the latter of which increases the risk of toxic acrylamide levels. The reasons people are hooked on coffee are not consistent, but here are some interesting coffee statistics:7
By Dr. Mercola
Limiting your net carbohydrate intake is an effective way to normalize your weight and reduce your risk for metabolic syndrome, heart disease and many other chronic diseases. Net carbs are calculated by subtracting your net fiber in grams from your total carbohydrates in grams. The resulting number are your net carbohydrates. Your body needs fiber carbohydrates as a prebiotic to feed good bacteria and support a strong gut microbiome.
However, your body doesnt need carbohydrates from grains, breads and pastas. In fact, the reaction inside your body from these foods actually feeds the development of chronic illnesses, which makes the most recent media headlines even more dangerous to the future health of Americans.
Two studies in the past two years claim eating pasta doesnt sabotage weight loss efforts, but instead may actually help. In both cases, the headlines only tell half the story possibly even less. In the first study, published in Nutrition and Diabetes in 2016,1 the authors used two large data sets to evaluate the effect eating a side dish of pasta with a Mediterranean diet would have.
Nearly 20,000 people from two different ongoing research groups were recruited for the trial. Each person filled out a food questionnaire to determine what they ate and how much, and they had their weight, height, waist and hip measurements taken. The data demonstrated no correlation between eating pasta and being overweight. Instead, those who ate pasta along with a strict Mediterranean diet were more slender.2
What the headlines failed to communicate was the people who were more slender and eating pasta were already maintaining an overall healthy, balanced diet and ate only a small side dish of pasta, rather than pasta as the main meal. Instead, headlines read:3 Pasta Isn't Fattening, and Can Actually Help You Lose Weight, Study Finds Unfortunately, for those who get their news from the headlines, this is misleading as many foods eaten in very small portions are not as dangerous as portion sizes common in America.
The most recent headline asks you to believe eating pasta will help you lose weight based on a study in w...
By Dr. Mercola
Google and Facebook are two of the largest and clearest monopolies in the world, and between them, the harvesting of your personal information goes far beyond what most people realize was even possible.
Google catches every single thing you do online if youre using a Google-based feature, and Facebook has a record of everything youve ever said or liked. All of this data is being used to build very powerful personality profiles that are sold for profit and used in a variety of different ways. As previously reported by Gawker:1
Every word of every email sent through Gmail and every click made on a Chrome browser is watched by the company. We dont need you to type at all, [Google co-founder Eric] Schmidt once said. We know where you are. We know where youve been. We can more or less know what youre thinking about.
According to CNN Money,2 98 percent of Facebooks revenue comes from advertising, totaling $39.9 billion last year alone. The company is now facing a firestorm after The New York Times and British media outlets reported Cambridge Analytica used improperly gleaned data from 87 million Facebook users to influence American voters.3
In the video above, Cambridge Analytica data scientist Christopher Wylie, who blew the whistle on his employer, revealing the company built a system that could profile individual U.S. voters in order to target them with personalized political advertisements during the presidential campaign. As noted by CNN:
If the Cambridge Analytica scandal leads to tougher data protection regulations as some policymakers are demanding or puts people off sharing as much about themselves online, that could hurt Facebook's revenue, and that of all social media platforms.
Its important to realize that Google is also the worlds greatest artificial intelligence (AI) company, having purchased Deep Mind for $400 million several years ago. Deep Mind now employs over 700 AI researchers, the largest collection anywhere in the world.
They are respo...
Late [last] Wednesday, a coalition of environmental organizations and farmers represented by the Center for Food Safety (CFS) and Earthjustice filed new legal papers in federal court seeking the reversal of Scott Pruitt and the Trump Environmental Protection Agencys (EPA) approval of Dow Chemicals toxic pesticide, Enlist Duo. The novel pesticide is a combination of glyphosate and 2,4-D, to be sprayed over the top of corn, cotton and soybeans that are genetically engineered by Dow with resistance to both pesticides.
Our filing reveals that EPA approved Enlist Duo despite its significant harms to health, environment, farms, water, and endangered species, said Sylvia Wu, CFS attorney and counsel for the coalition. EPAs job is protecting the environment, human health, and farmers, not blindly do the bidding of pesticide companies. The court must stop its use.
Garcinia cambogia and apple cider vinegar are used by many people to assist with weight loss. Garcinia cambogia contains hydroxycitric acid (HCA) which some scientific research has linked to getting rid of body fat and speedy weight loss. Using garcinia cambogia to help lose weight became popular when Dr. Oz proclaimed that the supplement was the holy grail of fat loss.
However, research into the benefits of garcinia cambogia (GC) for dieting to lose weight is mixed. Some supplements containing garcinia cambogia have also been linked to some health concerns and side effects.
In this article, you will find out all you need to know about garcinia cambogia and apple cider vinegar. I will also look at what research actually says about garcinia cambogia as a weight-loss supplement. This will help you decide if you can safely use the apple cider vinegar diet with garcinia cambogia extract for weight loss.
Many people claim that the garcinia cambogia and apple cider vinegar diet may help you lose weight quicker. If you have done any internet search for garcinia and apple cider vinegar for weight loss, you will have discovered conflicting reports.
Dr. Oz was one of the first to popularize garcinia supplements for weight loss. Research seemed to point to the fact that garcinia cambogia extract really does work as a natural fat burner supplement. However, other studies have shown conflicting reports. Some studies seem to suggest that there is only a minimal effect or that it doesnt work at all. Later in the article you will find out what current research says about the safety and health benefits of garcinia cambogia.
To speed up weight loss using garcinia cambogia, many people use Braggs apple cider vinegar. This has become one of the most popular weight-loss methods. I will examine scientific research looking at if apple cider vinegar does help to lose weight. I will also discuss any side effects of these natural weight loss methods and what is the correct dosage to use garcinia cambogia for weight loss along with the apple cider vinegar diet.
Garcinia cambogia is a fruit that grows in south-east Asia and India and looks like a small pumpkin. According to the website ConsumerLab.com, garcinia cambogia extract is advertised as weight-loss supplement. The extract being used in these supplements is called hydroxycitric acid (HCA). The potential for garcinia cambogia pills to assist in losing weight and body fat is due to amounts of hydroxycitric acid (HCA)....
PMID: BMC Infect Dis. 2018 Apr 10 ;18(1):167. Epub 2018 Apr 10. PMID: 29636018 Abstract Title: Lactobacillus paracasei feeding improves the control of secondary experimental meningococcal infection in flu-infected mice. Abstract: BACKGROUND: The use of probiotics to improve anti-microbial defence, such as for influenza infections, is increasingly recommended. However, no data are available on the effect of probiotics on flu-associated secondary bacterial infections. There is strong evidence of a spatiotemporal association between influenza virus infection and invasive Neisseria meningitidis. We thus investigated the effect of feeding mice Lactobacillus paracasei CNCM I-1518 in a mouse model of sequential influenza-meningococcal infection.METHODS: We intranasally infected BALB/c mice with a strain of influenza A virus (IAV) H3N2 that was first adapted to mice. Seven days later, a secondary bacterial infection was induced by intranasal administration of bioluminescent N. meningitidis. During the experiment, mice orally received either L. paracasei CNCM I-1518 or PBS as a control. The effect of L. paracasei administration on secondary bacterial infection by N. meningitidis was evaluated.RESULTS: Oral consumption of L. paracasei CNCM I-1518 reduced the weight loss of infected mice and lowered the bioluminescent signal of infecting meningococci. This improvement was associated with higher recruitment of inflammatory myeloid cells, such as interstitial monocytes and dendritic cells, to the lungs.CONCLUSIONS: Our data highlight the role of the gut-lung axis. L. paracasei CNCM I-1518 may boost the defence against IAV infection and secondary bacterial infection, which should be further studied and validated in clinical trials.
PMID: Nutrients. 2018 Apr 12 ;10(4). Epub 2018 Apr 12. PMID: 29649103 Abstract Title: The Anti-Periodontitis Effects of Ethanol Extract Prepared Using Lactobacillus paracasei subsp. paracasei NTU 101. Abstract: Poor oral health and related diseases, including caries, periodontal disease, and oral cancer, are highly prevalent across the world, particularly in the elderly. This study aimed to investigate the anti-periodontitis activity of fermented skim milk produced using the promising probioticsubsp.NTU 101 (NTU101FM). An initial analysis found that an ethanol extract of NTU101FM displayed anti-oxidative activities. Further investigation of pathogen growth inhibition zones, minimum inhibitory concentrations (MICs), and minimum bactericidal concentrations (MBCs) revealed that the NTU101FM ethanol extract also had anti-periodontal pathogen activities. In addition, the NTU101FM ethanol extract significantly decreased the release of pro-inflammatory cytokines induced by lipopolysaccharide (LPS) in RAW 264.7 macrophage cells. Finally, the NTU101FM ethanol extract was found to inhibit receptor activator of nuclear factor-B ligand (RANKL)-induced osteoclast differentiation by reducing tartrate-resistant acid phosphatase (TRAP) activity and the number of TRAP-positive multinucleated osteoclasts. In summary, our study demonstrated that ethanol extract prepared from NTU101FM has potential use as an anti-periodontitisagent.
PMID: Cell Physiol Biochem. 2017 ;42(6):2330-2341. Epub 2017 Aug 18. PMID: 28848081 Abstract Title: Bifidobacterium Infantis Ameliorates Chemotherapy-Induced Intestinal Mucositis Via Regulating T Cell Immunity in Colorectal Cancer Rats. Abstract: BACKGROUND/AIMS: Intestinal mucositis (IM) is a commonly encountered side effect in cancer patients receiving chemotherapy. This study aimed to investigate the effect of Bifidobacterium infantis (B. infantis) in attenuating the severity of chemotherapy-induced intestinal mucositis by regulating the T cell subsets in rats with colorectal cancer (CRC).METHODS: Thirty male Sprague-Dawley (SD) rats were injected dimethyl hydrazine (DMH) subcutaneously for 10 weeks, and then injected SW480 cells in rectal mucosa to create a CRC model, and the rats were randomly divided into three groups: Control group (saline + saline), Chemotherapy group (saline + 5-FU+Oxaliplatin), B. infantis group (B. infantis + 5-FU+Oxaliplatin). IM was evaluated based on diarrhea severity, intestinal villus height, crypt depth, pro-inflammatory cytokines (IL-6, IL-1, TNF-), T cell subsets (CD4+ IL17A+ cells and CD4+ CD25+ Foxp3+ Tregs) and related cytokine profiles.RESULTS: The results showed that the B. infantis group demonstrated a higher body weight (BW) and intestinal villus height and a deeper crypt depth compared to the Chemotherapy group. The level of IL-6, IL-1 and TNF- which increased by chemotherapy, was lowered by B. infantis administration. Real time reverse transcription- polymerase chain reaction (RT-PCR) showed B. infantis reduced relative expression of Th17 and Th1 cells related cytokines, and increased relative expression of CD4+ CD25+ Foxp3+Tregs related cytokines. Furthermore, Flow cytometry analysis showed B. infantis reduced CD4+ IL17A+ cells and increased CD4+ CD25+ Foxp3+ Tregs in mesenteric lymph nodes (MLNs) compared to the Chemotherapy group.CONCLUSION: B. infantis effectively attenuates chemotherapy-induced intestinal mucositis by decreasing Th1 and Th17 response and increasing CD4+ CD25+ Foxp3+ Tregs response.
PMID: Front Microbiol. 2017 ;8:1732. Epub 2017 Sep 11. PMID: 28959241 Abstract Title: Prophylactic Supplementation of5Protects Mice from Ovariectomy-Induced Exacerbated Allergic Airway Inflammation and Airway Hyperresponsiveness. Abstract: Asthma is a chronic inflammatory disease that affects more females than males after puberty, and its symptoms and severity in women change during menstruation and menopause. Recently, evidence has demonstrated that interactions among the microbiota, female sex hormones, and immunity are associated with the development of autoimmune diseases. However, no studies have investigated if therapeutic gut microbiota modulation strategies could affect asthma exacerbation during menstruation and menopause. Here we aimed to examine the preventive effects of a probiotic,5, on airway inflammation exacerbation in allergic ovariectomized mice. We first evaluated the gut microbiota composition and diversity in mice 10 days after ovariectomy. Next, we examined whether re-exposure of ovariectomized allergic mice to antigen (ovalbumin) would lead to exacerbation of lung inflammation. Finally, we evaluated the preventive and treatment effect of5on lung inflammation and airway hyperresponsiveness. Our results showed that whereas ovariectomy caused no alterations in the gut microbiota composition and diversity in this animal model, 10 days after ovariectomy, preventive use administration of5, rather than its use after surgery was capable of attenuate the exacerbated lung inflammation and hyperresponsiveness in ovariectomized allergic mice. This prophylactic effect of5involves acetate production, which led to increased fecal acetate levels and, consequently, increased Treg cells in ovariectomized allergic mice.
PMID: Cell Host Microbe. 2018 01 10 ;23(1):27-40.e7. Epub 2017 Dec 21. PMID: 29276171 Abstract Title: Bifidobacteria or Fiber Protects against Diet-Induced Microbiota-Mediated Colonic Mucus Deterioration. Abstract: Diet strongly affects gut microbiota composition, and gut bacteria can influence the colonic mucus layer, a physical barrier that separates trillions of gut bacteria from the host. However, the interplay between a Western style diet (WSD), gut microbiota composition, and the intestinal mucus layer is less clear. Here we show that mice fed a WSD have an altered colonic microbiota composition that causes increased penetrability and a reduced growth rate of the inner mucus layer. Both barrier defects can be prevented by transplanting microbiota from chow-fed mice. In addition, we found that administration of Bifidobacterium longum was sufficient to restore mucus growth, whereas administration of the fiber inulin prevented increased mucus penetrability in WSD-fed mice. We hypothesize that the presence of distinct bacteria is crucial for proper mucus function. If confirmed in humans, these findings may help to better understand diseases with an affected mucus layer, such as ulcerative colitis.
PMID: Curr Pharm Biotechnol. 2017 ;18(11):883-889. PMID: 29278212 Abstract Title: Immunomodulatory Effects of Bifidobacterium longum W11 Produced Exopolysaccharide on Cytokine Production. Abstract: BACKGROUND: Health benefits, including immune modulating capability, exerted by Bifidobacterium strains have been attributed to their exopolysaccharides (EPSs).OBJECTIVE: The effects of the purified EPS from B. longum W11 on cytokine production by peripheral blood mononuclear cells (PBMCs) alone or ConA-stimulated were investigated.METHOD: The production of IFN-, IL-1, IL-6 and IL-10 by PBMCs from healthy adult donors was analysed using purified EPS at two different concentrations (100 g/mL and 200 g/mL) and ConA, as an immunopotentiating marker. Moreover, the monosaccharide composition of the EPS from B. longum W11 was detected using HPLC analysis.RESULTS: The results demonstrated the ability of purified EPS to increase the production of the tested cytokines, except IL-10, in ConA-stimulated PBMCs. In not-stimulated-PBMCs, EPS increased the production of IL-6 (at 200g/mL) and IL-10 (at 100 g/mL). The HPLC analysis showed the presence of main monomers, galactose and glucose (ratio 1:1 wt/wt), and small amount of rhamnose.CONCLUSION: The results of this study demonstrate the ability of the EPS produced by B. longum W11 to interact in vitro with the human PBMCs, showing an immune-regulatory profile alone and an immune stimulatory profile in ConA-stimulated PBMCs. This suggests putative applications for the EPS from B. longum W11 in different pathological conditions.
PMID: J Microbiol Methods. 2018 Feb ;145:87-92. Epub 2018 Jan 9. PMID: 29329748 Abstract Title: Oral Bifidobacterium longum expressing GLP-2 improves nutrient assimilation and nutritional homeostasis in mice. Abstract: Bifidobacterium has been developed for the oral delivery of peptides and has the added beneficial effect on our bodies through its probiotic properties. Here, we utilize Bifidobacterium as a delivery system to orally deliver Glucagon like peptide-2 (GLP-2). We constructed vector derived from pET-31b(+) to construct a Bifidobacterium longum expressing GLP-2. We then determined the bioactivity of recombinant Bifidobacterium in Caco-2 cells. Finally, we quantified newly synthesized ApoB48 and chylomicron production in mice infused with exogenous GLP-2 or Bifidobacterium expressing GLP-2. Results based on secretion of the triglyceride (TG)-rich lipoprotein (TRL)-ApoB48 and secretion of chylomicron revealed that recombinant Bifidobacterium was efficient in treating intestinal dysfunction,suggesting an alternative way to use Bifidobacterium as a delivery system to deliver GLP-2 for gastrointestinal nutrition coordination.
PMID: Biosci Biotechnol Biochem. 2018 Mar 20:1-8. Epub 2018 Mar 20. PMID: 29557273 Abstract Title: Oral supplementation of Bifidobacterium longum strain BR-108 alters cecal microbiota by stimulating gut immune system in mice irrespectively of viability. Abstract: Effect on cecal microbiota and gene expression of various cytokines in ileal Peyer's patches and cecal tissues were compared between viable and heat-killed Bifidobacterium longum strain BR-108 (BR-108) using a mouse model. Irrespectively of viability, oral supplementation of BR-108 altered the cecal microbiota and stimulated gene expression of cytokines such as IL-6 and IL-10 in ileal Peyer's patches and cecal tissue of mice. In addition, BR-108 supplementation significantly affected the relative abundance of bacterial genera and family, Oscillospira, Bacteroides and S24-7. The abundance of these bacterial genera and family strongly correlated with gene expression induced by BR-108. This study demonstrated that the effect of heat-killed BR-108 on the mouse cecal microbiota is similar to that of viable BR-108, most likely due to stimulation of the gut immune system by both heat-killed and viable BR-108 is also similar.
PMID: J Microbiol Biotechnol. 2018 Mar 16. Epub 2018 Mar 16. PMID: 29551020 Abstract Title: Anti-obese Effects of a Mixture of Fermented Ginseng,BORI, andCH88 in high-fat diet-fed mice. Abstract: Ginseng and probiotics have anti-obese effects in mice fed a high-fat diet(HFD). Absorption of ginsenoside and colonization of probiotics occur in the intestine. In this study, the mixture of fermented ginseng and two probiotics,BORI andCH88, was administered to HFD fed mice for 9 weeks. The mixture significantly suppressed weight gain (
PMID: Oncol Lett. 2018 Apr ;15(4):6043-6049. Epub 2018 Feb 15. PMID: 29556318 Abstract Title: Dammarane-type triterpene ginsenoside-Rg18 inhibits human non-small cell lung cancer A549 cell proliferation via Gphase arrest. Abstract: A previous study reported that a novel dammarane-type triterpene saponin, ginsenoside-Rg18, derived from the root, displayed hydroxyl radical scavenging, anti-bacterial and cytotoxic activities. However, the underlying molecular mechanisms of its anti-proliferative effect on non-small cell lung cancer (NSCLC) A549 cells remains unclear. In the present study, it was determined that Rg18 inhibited the proliferation of A549 cells with a half-maximal inhibitory concentration of 150M. Flow cytometry analysis indicated that cell cycle progression was blocked by Rg18 at Gphase in A549 cells, which was accompanied by downregulation of cyclin-dependent kinase 2 (CDK2), CDK4, CDK6, cyclin D1, cyclin D2, cyclin E and phosphorylated retinoblastoma protein expression at the protein level. In addition, the CDK inhibitors (CDKNs), CDKN1A and CDKN1B, were upregulated following Rg18 treatment. Furthermore, Rg18 treatment resulted in the intracellular accumulation of reactive oxygen species (ROS), and a dose-dependent inhibition of p38 mitogen activated protein kinase (p38), c-Jun N-terminal kinase (JNK) and nuclear factor-B (NF-B)/p65 phosphorylation. Taken together, Rg18-mediated Gphase arrest was closely associated with the generation of intracellular ROS, and p38, JNK and NF-B/p65 inhibition in A549 human NSCLC cells.
PMID: Cell Physiol Biochem. 2018 Mar 16 ;45(6):2548-2559. Epub 2018 Mar 16. PMID: 29558748 Abstract Title: Ginsenoside 20(S)-Rg3 Inhibits the Warburg Effect Via Modulating DNMT3A/ MiR-532-3p/HK2 Pathway in Ovarian Cancer Cells. Abstract: BACKGROUND/AIMS: The Warburg effect is one of the main energy metabolism features supporting cancer cell growth. 20(S)-Rg3 exerts anti-tumor effect on ovarian cancer partly by inhibiting the Warburg effect. microRNAs are important regulators of the Warburg effect. However, the microRNA regulatory network mediating the anti-Warburg effect of 20(S)-Rg3 was largely unknown.METHODS: microRNA deep sequencing was performed to identify the 20(S)-Rg3-influenced microRNAs in SKOV3 ovarian cancer cells. miR-532-3p was overexpressed by mimic532-3p transfection in SKOV3 and A2780 cells or inhibited by inhibitor532-3p transfection in 20(S)-Rg3-treated cells to examine the changes in HK2 and PKM2 expression, glucose consumption, lactate production and cell growth. Dual-luciferase reporter assay was conducted to verify the direct binding of miR-532-3p to HK2. The methylation status in the promoter region of pre-miR-532-3p gene was examined by methylation-specific PCR. Expression changes of key molecules controlling DNA methylation including DNMT1, DNMT3A, DNMT3B, and TET1-3 were examined in 20(S)-Rg3-treated cells. DNMT3A was overexpressed in 20(S)-Rg3-treated cells to examine its influence on miR-532-3p level, HK2 and PKM2 expression, glucose consumption and lactate production.RESULTS: Deep sequencing results showed that 11 microRNAs were increased and 9 microRNAs were decreased by 20(S)-Rg3 in SKOV3 cells, which were verified by qPCR. More than 2-fold increase of miR-532-3p was found in 20(S)-Rg3-treated SKOV3 cells. Forced expression of miR-532-3p reduced HK2 and PKM2 expression, glucose consumption and lactate production in SKOV3 and A2780 ovarian cancer cells. Inhibition of miR-532-3p antagonized the suppressive effect of 20(S)-Rg3 on HK2 and PKM2 expression, glucose consumption and lactate production in ovarian cancer cells. Dual-luciferase reporter assay showed that miR-532-3p directly suppressed HK2 rather than PKM2. miR-532-3p level was controlled by the methylation in the promoter region of its host gene. 20(S)-Rg3 inhibited DNMT3A expression while exerted insignificant effect on DNMT1, DNMT3B and TET1-3. 20(S)-Rg3 reversed DNMT3A-mediated methylation in the promoter of the host gene of miR-532-3p, and thus elevated miR-532-3p level followed by suppression of HK2 and PKM2 expression, glucose consumption and lactate production.CONCLUSIONS: 20(S)-Rg3 modulated microRNAs to exert the anti-tumor effect in ovarian cancer. 20(S)-Rg3 lessened the DNMT3A-mediated methylation and promoted the suppression of miR-532-3p on HK2 to antagonize the Warburg effect of ov...
PMID: J Pharm Pharmacol. 2018 Mar 25. Epub 2018 Mar 25. PMID: 29574918 Abstract Title: Ginsenoside Rb1 inhibits autophagy through regulation of Rho/ROCK and PI3K/mTOR pathways in a pressure-overload heart failure rat model. Abstract: OBJECTIVE: This study was designed to explore the relationship between ginsenoside Rb1 (Grb1) and high-load heart failure (HF) in rats.METHODS: The parameters of cardiac systolic function (left ventricular posterior wall thickness (LVPWT), left ventricular internal diastolic diameter (LVID), fraction shortening (FS) and mitral valves (MVs)) of rat hearts in each group were inspected by echocardiogram. The expressions of rat myocardial contractile proteins, autophagy-related proteins and the activation of Rho/ROCK and PI3K/mTOR pathways were detected by Western blot.KEY FINDINGS: LVPWT, FS, MVs and the expression of myocardial contractile proteins-MHC, apoptosis-related proteins Bcl-2 and signalling pathway involved proteins pAkt and mTOR were significantly reduced in the HF, HF+5 mg/kg Grb1 (HF+Grb1-5) and HF+Grb1+arachidonic acid (AA) groups with LVID, -MHC, cell apoptosis, cell autophagy and Rho/ROCK significantly increased comparedwith the control group, of which the tendency was contrary to the HF+20 mg/kg Grb1 (HF+Grb1-20) group compared with the HF group (P
PMID: J Cell Mol Med. 2018 Mar 22. Epub 2018 Mar 22. PMID: 29566305 Abstract Title: Ginsenoside Rg3 stereoisomers differentially inhibit vascular smooth muscle cell proliferation and migration in diabetic atherosclerosis. Abstract: Ginsenoside 20(R/S)-Rg3, as a natural peroxisome proliferator-activated receptor gamma (PPAR) ligand, has been reported to exhibit differential biological effects. It is of great interest to understand the stereochemical selectivity of 20(R/S)-Rg3 and explore whether differential PPAR activation by Rg3 stereoisomers, if it exists, could lead to differential physiological outcome and therapeutic effects in diabetic atherosclerosis. Here, we investigated the binding modes of 20(R/S)-Rg3 stereoisomers in the PPAR ligand-binding domain (PPAR-LBD) using molecular modelling and their effects on smooth muscle cell proliferation and migration induced by advanced glycation end products (AGEs). The results revealed that 20(S)-Rg3 exhibited stronger antiproliferative and antimigratory effects due to stronger PPAR activation. To validate the in vitro results, we used a mice model with diabetic atherosclerosis and obtained that 20(S)-Rg3 markedly reduced the plaque size secondaryto reducing the proliferation and migration of VSMCs, while the plaques were more stable due to improvements in other plaque compositions. The results shed light on the structural difference between Rg3 stereoisomers that can lead to significant differential physiological outcome, and the (S)-isomerseems to be the more potent isomer to be developed as a promising drug for diabetic atherosclerosis.
PMID: Biochem Biophys Res Commun. 2018 May 5 ;499(2):381-388. Epub 2018 Mar 26. PMID: 29577907 Abstract Title: Possible role of ginsenoside Rb1 in skin wound healing via regulating senescent skin dermal fibroblast. Abstract: Cellular senescence suppresses cancer by inducing irreversible cell growth arrest. Nevertheless, senescent cells is proposed as causal link with aging and aging-related pathologies. The physiological beneficial functions of senescent cells are still of paucity. Here we show that senescent human dermal fibroblast accelerates keratinocytes scratch wound healing and stimulates differentiation of fibroblast. Using oxidative stress (100MHOexposure for 1h) induction, we successfully triggered fibroblast senescence and developed senescence associated secretory phenotype (SASP). The induction of SASP was regulated by p38MAPK/MSK2/NF-B pathway. Interestingly, inhibition of p38MAPK activation only partially suppressed SASP. However, SASP was significantly inhibited by SB747651A, a specific MSK inhibitor. Additionally, we demonstrate that SASP stimulates migration of keratinocytes and myofibroblast transition of fibroblast, through fold-increased secretion of growth factors, platelet-derived growth factor AA (PDGF-AA) and AB (PDGF-AB), transforming growth factor beta 1 (TGF-1) and beta 2 (TGF-2), vascular endothelial growth factor A (VEGF-A) and D (VEGF-D), vascular endothelial growth factor receptor 2 (VEGFR2) and 3 (VEGFR3). Importantly, we also confirmed ginsenoside Rb1 promoted SASP-mediated healing process via p38MAPK/MSK2/NF-B pathway. The results pointed to senescent fibroblast as a potential mechanism of wound healing control in human skin. Further, it provided a candidate targeted for wound therapy.
PMID: Biochem Biophys Res Commun. 2018 Mar 31. Epub 2018 Mar 31. PMID: 29596831 Abstract Title: The anti-tumor effect of ginsenoside Rh4 in MCF-7 breast cancer cells in vitro and in vivo. Abstract: Breast cancer is a tremendous threat to humans in many countries, and thus we need to find safe and effective drugs for treatment. Ginsenoside Rh4 has been reported to be present in processed ginseng. However, few studies have focused on its anti-tumor activity. In this study, we investigated the inhibitory effects of ginsenoside Rh4 on MCF-7 breast cancer cells and the pathways that promote apoptosis in vitro. To study the effect of ginsenoside Rh4 in vivo, xenograft models were randomly divided into 3 groups (the control group, 10mg/kg/d Rh4, 20mg/kg/d Rh4, n=10 per group), the ginsenoside Rh4 injection method was i.p. The results showed that ginsenoside Rh4 effectively inhibited proliferation, arrested the cell cycle in S phase and induced apoptosis in MCF-7cells by flow cytometry. Morphological changes caused by ginsenoside Rh4-induced apoptosis were also observed by Hoechst 33342 staining. Western-blot analyses indicated that the apoptosis-inducing effects of ginsenosideRh4 were associated with the external pathway by decreasing Bcl-2, increasing Bax, and activating caspase-8, -3 and PARP. Moreover, ginsenoside Rh4 significantly inhibited the growth of MCF-7 tumor cells in vivo. These results suggested that ginsenoside Rh4 could be a potentially effective anti-tumor drug for breast cancer.
PMID: Food Funct. 2018 Apr 10. Epub 2018 Apr 10. PMID: 29632923 Abstract Title: Anti-anemia effects of ginsenoside Rk3 and ginsenoside Rh4 on mice with ribavirin-induced anemia. Abstract: Ginsenoside Rk3 (Rk3) and ginsenoside Rh4 (Rh4) have been reported to have therapeutic effects on a variety of diseases including some hematological diseases. The aim of the present study was to treat ribavirin-induced anemia in mice with Rk3 and Rh4 and explore the therapeutic mechanisms. All the experimental mice were randomly divided into 10 groups (the normal group, the control group, the model group, the erythropoietin (EPO) group, and 6 groups of different doses of Rk3 and Rh4, n = 10 per group). The changes in the hemograms, the content of cytokines, the number of hematopoietic cells and viscera damage were observed in the experiment. The results showed that the red blood cell count, the hemoglobin amount and the hematocrit of mice in the ginsenoside groups could be restored to normal values in a short time, and the effects of both Rk3 and Rh4 are obviously better than those of EPO. Moreover, some positive regulators (EPO, erythroid transcription factor-1, and interleukin-3) related to hematopoiesis increased and some negative regulators (interferon- and tumour necrosis factor-) decreased in vivo after the injection of Rk3 or Rh4. These two ginsenosides did little damage to viscera and produced different degrees of increase in the hematopoietic cells in the bone marrow and spleen, which indicated that Rk3 promoted bone marrow and extramedullary haematopoiesis while Rh4 promoted extramedullary hematopoiesis. Therefore, both Rk3 and Rh4 could be considered new therapeutic drugs for treating anemia.
PMID: Int Immunopharmacol. 2018 Apr 2 ;59:21-30. Epub 2018 Apr 2. PMID: 29621733 Abstract Title: 20(R)-ginsenoside Rg3, a rare saponin from red ginseng, ameliorates acetaminophen-induced hepatotoxicity by suppressing PI3K/AKT pathway-mediated inflammation and apoptosis. Abstract: Although ginsenoside Rg3 was isolated as a major component of Korea red ginseng and confirmed to exert potential hepatoprotective effect on acetaminophen (APAP)-induced liver injury via induction of glutathione S-transferase (GST) in vitro, thein vivo hepatoprotective effect of Rg3 and the underlying molecular mechanism of action remain unclear. The current study was aimed to explore whether 20(R)-Ginsenoside Rg3 (20(R)-Rg3) could alleviate acetaminophen-induced liver injury in mice and to determine the involvement of PI3K/AKT signaling pathway. Our findings demonstrated that a single injection of APAP (250mg/kg) increased the levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), tumor necrosis factor- (TNF-), and interleukin-1 (IL-1); such increases were attenuated by pretreatment of mice with 20(R)-Rg3 for seven days. The depletion of glutathione (GSH), generation ofmalondialdehyde (MDA) and the over expression of cytochrome P450 E1 (CYP2E1) and 4-hydroxynonenal (4-HNE) caused by APAP exposure were also inhibited by 20(R)-Rg3 pretreatment. Moreover, 20(R)-Rg3 pretreatment significantly alleviated APAP-induced apoptosis, necrosis, and inflammatory infiltrationin liver tissues. Importantly, 20(R)-Rg3 effectively attenuated APAP-induced liver injury in part via activating PI3K/AKT signaling pathway. In summary, 20(R)-Rg3 exerted liver protection against APAP-caused hepatotoxicity evidenced by inhibition of oxidative stress and inflammatory response, alleviation of hepatocellular necrosis and apoptosis via activation of PI3K/AKT signaling pathway, showing potential as a novel therapeutic agent to prevent liver damage.
PMID: Am J Transl Res. 2018 ;10(3):796-805. Epub 2018 Mar 15. PMID: 29636869 Abstract Title: Ginsenoside Rb1 inhibit apoptosis in rat model of Alzheimer's disease induced by A. Abstract: Alzheimer's disease (AD) is the most common form of dementia and lacks disease-altering treatments. Ginsenoside Rb1 (GsRb1), the key active compounds of ginsenoside found in ginseng. The present study aimed to determine whether GsRb1 could prevent cognitive deficit and take neuroprotective effects in A-induced rat model through apoptotic signaling pathway. Injection of soluble Ainto the hippocampus caused impairment in learning and memory. Daily administration of Rb1 (12.5, 25, and 50 mg/kg,) for 14 consecutive days. All rats were tested for their capabilities of spatial navigation and memorization by Morris water maze. Apoptosis was tested using TUNEL staining in hippocampus neuronal cells. RT-PCR, immunohistochemical staining and western blotting were employed to confirm the expressions of Bcl-2, Bax and Cleaved Caspase-3. The results showed that Rb1 administration could prevent cognitive deficit, and significantly decreased the levels of Bax and Cleaved Caspase-3 meanwhile up regulation the level of Bcl-2 in the hippocampus. We suggest that GsRb1 may be effective for preventing or slowing the development of Alzheimer's disease, which improving cognitive and memory functions by inhibiting the levels of pro-apoptosis mediators and improving the levels of anti-apoptosis mediators in the rat brain.
PMID: Mol Med Rep. 2018 May ;17(5):6269-6276. Epub 2018 Mar 1. PMID: 29512726 Abstract Title: Ginsenoside Rg1 ameliorates testicular senescence changes in Dgalinduced aging mice via antiinflammatory and antioxidative mechanisms. Abstract: With the growing population, aging, extended lifespans and anti-aging have become popular areas of research in the life and social sciences. With increasing age, the structure and function of the testes, the spermatogenetic and androgenproducing organ in the male reproductive system, gradually declines. Ginsenoside Rg1 is an extract of Panax ginseng in traditional Chinese medicine. The extract facilitates antiaging through its antiinflammatory and antioxidant properties. However, it has not been reported whether ginsenoside Rg1 delays testicular aging. The present study established Dgalactose (Dgal)induced aging mouse models to examine the protective effects of ginsenoside Rg1 on the structure and function of the testes, and the underlying mechanism. A total of 60 healthy specific pathogenfree male C57BL/6 mice were randomly divided into four groups: Control group; Rg1 group; Dgal + Rg1 group; and Dgal group. The tissues of the mice were used for further experiments. The present study further investigated the effects of Rg1 on the volume of serum testosterone, the testicular index, testicular microscopic structures, the senescence of spermatogenetic cells, the apoptosis of spermatogenetic cells, the activity of the antioxidant enzymes, the levels of inflammatory cytokines, and the levels of Sphase kinaseassociated protein (p19), cyclindependent kinase inhibitor 1 (p21) and cellular tumor antigen p53 (p53) in Dgalinduced aging mice. In general, compared with the Dgal group, the treatment of Rg1 increased the testis index, serum testosterone level and the active content of superoxide dismutase and the total antioxidant capacity. The percentage of senescenceassociated galactosidasepositive cells, the level of apoptosis and the volume of methane dicarboxylic aldehyde, tumor necrosis factor, interleukin (IL)1 and IL6 in testicular tissues were significantly decreased, and the expression of p19, p53 and p21 was downregulated due to the treatment with Rg1. The results of the present study demonstrated that ginsenoside Rg1 was able to protect the testes against Dgalinduced aging in mice. In addition, the protective effect of Rg1 may be achieved via antioxidation and downregulation of the p19/p53/p21 signaling pathway.
PMID: Braz J Med Biol Res. 2018 Mar 1 ;51(4):e7139. Epub 2018 Mar 1. PMID: 29513799 Abstract Title: Ginsenoside Rg1 inhibits dietary-induced obesity and improves obesity-related glucose metabolic disorders. Abstract: Obesity and its consequent type 2 diabetes are significant threats to global health. Emerging evidence indicates that ginsenosides from ginseng (Panax ginseng) have anti-diabetic activity. We hypothesized that ginsenosides Rg1 could suppress dietary-induced obesity and improve obesity-related glucose metabolic disorders. Our results showed that ginsenoside Rg1 attenuated dietary-induced body weight gain and fat accumulation in white adipocyte tissue of mice fed a high-fat diet. Furthermore, we found that ginsenosides Rg1 not only decreased fasting glucose concentration and the 2-h postprandial glucose concentration, but also improved insulin resistance and glucose intolerance in those mice. Ginsenoside Rg1 also activated the AMPK pathway in vitro and in vivo and increased plasma membrane translocation of GLUT4 in C2C12 skeletal muscle cells. In conclusion, our observations suggested that ginsenoside Rg1 inhibited dietary-induced obesity and improved obesity-related insulin resistance and glucose intolerance by activation of the AMPK pathway.
PMID: Int Immunopharmacol. 2018 Mar 19 ;58:94-102. Epub 2018 Mar 19. PMID: 29567591 Abstract Title: Protective effects of ginsenoside Rg1 on splenocytes and thymocytes in an aging rat model induced by d-galactose. Abstract: Physiological aging is associated with a range of medical problems. However, the treatment of aging-associated diseases and prolonging human life are vital to our current aging societies. Panax ginseng, a traditional Chinese medicine, has been shown to have anti-oxidative and anti-aging effects. In the current study, aging rats induced by d-galactose were administered ginsenoside Rg1, then splenocytes and thymocytes were extracted and changes in activity were detected. The results demonstrated that compared with the d-gal group, the level of advanced glycation end products (AGE), the ratio of splenocytes and thymocytes in G0 phase (%), and apoptosis (%) of splenocytes and thymocytes, the ratio (%) of SA-gal positive splenocytes and thymocytes, the content of reactive oxygen species (ROS) and malondialdehyde (MDA), the ratio of glutathione (GSH) to oxidized glutathione (GSSG) and senescence-associated protein expression were significantly decreased and the index of the spleen and thymus, the proportion of white pulp in the spleen, the proportion of cortex in the thymus, the content of interleukin-2 (IL-2), interleukin-6 (IL-6), granulocyte-macrophage colony stimulating factor (GM-CSF), tumor necrosis factor- (TNF-), the activities of superoxide dismutase (SOD), and the proliferative capacity of splenocytes and thymocytes were increased in the Rg1+ d-gal group. These findings demonstrated that ginsenoside Rg1 may antagonize spleen and thymus damage in d-galactose-induced aging rats by alleviating oxidative stress injury and down-regulating the expression of senescence-associated protein.
PMID: Biochem Biophys Res Commun. 2018 Apr 11. Epub 2018 Apr 11. PMID: 29654764 Abstract Title: Rg1 inhibits high glucose-induced mesenchymal activation and fibrosis via regulating miR-2113/RP11-982M15.8/Zeb1 pathway. Abstract: Recent study has showed that Ginsenoside Rg1, the mian active compound of Panax ginseng, could ameliorate oxidative stress and myocardial apoptosis in diabetes mellitus. However, the roles and mechanisms of Rg1 in proliferative diabetic retinopathy (PDR) are still unclear. In the present study, we aimed to investigate the effects of Rg1 on mesenchymal activation of high-glucose (HG) cultured mller cells. High glucose conditions up-regulate MMP-2, MMP-9 and down-regulate TIMP-2, and promote mesenchymal activation in Mller cells. And Rg1 inhibits the HG-induced mesenchymal activation and HG-increased MMP-2 and MMP-9 and HG-decreased TIMP-2 in Mller cells. HG up-regulates Zeb1 and lncRNA RP11-982M15.8, and down-regulates miR-2113, and Rg1 inhibits these effects of HG. Both inhibition of miR-2113 and over-expression of RP11-982M15.8 significantly restored the HG induced mesenchymal activasion. Taken together, our findings suggested that Rg1 inhibited HG-induced mesenchymal activation and fibrosis via regulating miR-2113/RP11-982M15.8/Zeb1 pathway.
PMID: Biol Pharm Bull. 2018 ;41(4):585-591. PMID: 29607931 Abstract Title: Ginsenoside-Rg1 Protects against Renal Fibrosis by Regulating the Klotho/TGF-1/Smad Signaling Pathway in Rats with Obstructive Nephropathy. Abstract: Ginsenoside-Rg1 (G-Rg1) is an agent isolated from Panax ginseng that exerts anti-fibrotic effects; however, the mechanism is still unclear. Herein, we investigated whether G-Rg1 administration can mitigate or reverse unilateral ureteral obstruction (UUO)-induced renal fibrosis by regulating the Klotho/transforming growth factor (TGF)-1/Smad signaling pathway in rats. Sprague-Dawley male rats were subjected to UUO, and rats in the treatment group were administered G-Rg1 or G-Rg1 plus Klotho short hairpin RNA interference (shRNA), while rats in the control and model groups were administered vehicle for 14d. Epithelial-mesenchymal transition (EMT) biomarkers and Klotho/TGF-1 signaling molecules were examined by immunohistochemistry, quantitative real-time PCR and Western blotting. Immunohistochemistry showed that UUO induced increased pro-fibrotic TGF-1 expression, overexpression of the mesenchymal marker, -smoothmuscle actin (-SMA), and suppression of the epithelial marker, E-cadherin. Moreover, Western blotting analysis indicated that UUO promoted TGF-1 and phosphorylated Smad3 (p-Smad3) expression (p
PMID: Food Chem. 2017 Oct 15 ;233:429-433. Epub 2017 Apr 20. PMID: 28530594 Abstract Title: Mushrooms: A rich source of the antioxidants ergothioneine and glutathione. Abstract: While mushrooms are the highest dietary source for the unique sulfur-containing antioxidant ergothioneine, little is known regarding levels of the major biological antioxidant glutathione. Thus, our objectives were to determine and compare levels of glutathione, as well as ergothioneine, in different species of mushrooms. Glutathione levels varied>20-fold (0.11-2.41mg/gdw) with some varieties having higher levels than reported for other foods. Ergothioneine levels also varied widely (0.15-7.27mg/gdw) and were highly correlated with those of glutathione (r=0.62, P
PMID: Pol J Microbiol. 2017 Jul 6 ;66(2):201-207. PMID: 28735315 Abstract Title: The Diversity, Growth Promoting Abilities and Anti-microbial Activities of Bacteria Isolated from the Fruiting Body of Agaricus bisporus. Abstract: Agaricus bisporus plays an important role in ecological processes and is one of the most widely cultivated mushrooms worldwide. Mushroom growth-promoting bacteria have been isolated from casing soil and compost, but microorganisms in the fruiting body have received only a little attention. To get an overview of phylogenetic diversity of microorganisms in the fruiting body of A. bisporus, as well as to screen antimicrobial and mushroom growth-promoting strains, and eventually intensify mushroom production, we isolated and characterized microorganisms from the fruiting body of A. bisporus. In total, 55 bacterial strains were isolated, among which nine isolates represented Actinomycetes. All the isolates were analyzed by 16S rRNA gene RFLP and sixteen representative strains by 16S rRNA gene sequencing. According to the phylogenetic analysis, eleven isolates represented the Gram-positive Bacillus, Lysinibacillus, Paenibacillus, Pandorea and Streptomyces genera, and five isolates belonged to the Gram-negative Alcaligenes and Pseudomonas genera. The bacteria isolated from the fruiting body of A. bisporus had broad-spectrum antimicrobial activities and potential mushroom growth-promoting abilities.
PMID: Evid Based Complement Alternat Med. 2017 ;2017:7298683. Epub 2017 Aug 22. PMID: 29104605 Abstract Title: The Antioxidative, Antiaging, and Hepatoprotective Effects of Alkali-Extractable Polysaccharides by. Abstract: The aim of this work was designed to investigate the antioxidant, antiaging, and hepatoprotective effects of alkali-extractable polysaccharides (AlAPS) and their three purified fractions (AlAPS-1, AlAPS-2, and AlAPS-3) fromin D-galactose induced aging mice. Forantioxidant analysis, both AlAPS and its fractions exhibited moderate reducing power, Fe-chelating activities, and potent scavenging activities on hydroxyl and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals. Theresults demonstrated that the polysaccharides, especially AlAPS-2, showed potential antiaging and hepatoprotective effects by enhancing the antioxidant status, decreasing serum hepatic enzyme activities, and improving the lipid metabolism. This study suggested that the polysaccharides extracted and purified fromcould be exploited as a potent dietary supplement to attenuate aging and prevent age-related diseases.
PMID: PLoS One. 2015 ;10(10):e0140355. Epub 2015 Oct 15. PMID: 26468946 Abstract Title: Antibacterial, Antiradical Potential and Phenolic Compounds of Thirty-One Polish Mushrooms. Abstract: BACKGROUND: Among many sources of natural bioactive substances, mushrooms constitute a huge and almost unexplored group. Fungal compounds have been repeatedly reported to exert biological effects which have prompted their use in pharmaceutical and cosmetic industry. Therefore, the aim of this study was analysis of chemical composition and biological activity of 31 wild growing mushroom species (including saprophytic and parasitic) from Poland.METHODS: Qualitative and quantitative LC-ESI-MS/MS analysis of fourteen phenolic acids in the mushrooms analysed was performed. Moreover, total phenolic content was determined by the modified Folin-Ciocalteau method. Antioxidative activity of ethanolic extracts towards DPPH free radical was examined. Antibacterial activity against Gram-positive (S. epidermidis, S. aureus, B. subtilis, M. luteus) and Gram-negative (E. coli, K. pneumoniae, P. aeruginosa, P. mirabilis) microbial strains was analyzed.RESULTS: As a result, the first such broad report on polyphenolic composition, antiradical and antimicrobial potential of wild growing Polish mushrooms was developed. Mushroom extracts were found to contain both benzoic (protocatechuic, 4-OH-benzoic, vanillic, syringic) and cinnamic acid derivatives (caffeic, p-coumaric, ferulic). Total phenolic content in mushrooms ranged between 2.79 and 53.13 mg gallic acid equivalent /g of dried extract in Trichaptum fuscoviolaceum and Fomes fomentarius, respectively. Fungi showed much differentiated antiradical activity, from highly active F. fomentarius to poorly effective Russula fragilis (IC50 1.39 to 120.54 mg per mg DPPH, respectively). A quite considerable relationship between phenolic content and antiradical activity has been demonstrated. Mushrooms varied widely in antimicrobial potential (MIC from 0.156 to 5 mg/ml). Generally, a slightly higher activity against Gram-positive than Gram-negative strains was observed. This is the first study concerning the chemical composition and biological activity of the majority of investigated species.
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PMID: Int Immunopharmacol. 2018 Jan ;54:286-295. Epub 2017 Nov 24. PMID: 29175507 Abstract Title: The mast cell stabilizing activity of Chaga mushroom critical for its therapeutic effect on food allergy is derived from inotodiol. Abstract: While an anti-allergic effect of Chaga mushroom (Inonotus obliquus) has been indicated, its therapeutic effect on allergy and immunoregulatory mechanisms and chemical constituents directly responsible for that are hardly known. We examined the effect of 70% ethanol extract of Chaga mushroom (EE) and its dichloromethane (DF) and aqueous (AF) fractions using a mouse model of chicken ovalbumin (cOVA)-induced food allergy, and found that only EE and DF ameliorated allergy symptoms to a significant extent. The in vivo mast cell-stabilizing activity was also found only in EE and DF whereas the activities to suppress Th2 and Th17 immune responses and cOVA-specific IgE production in the small intestine were observed in all three treatment regimens, implying that inhibition of the mast cell function by lipophilic compounds was vital for the therapeutic effect. Results also indicated that inotodiol, a triterpenoid predominantly present in DF, played an active role as a mast cell stabilizer.
PMID: Biomed Pharmacother. 2018 Mar ;99:142-150. PMID: 29331760 Abstract Title: Inotodiol suppresses proliferation of breast cancer in rat model of type 2 diabetes mellitus via downregulation of-catenin signaling. Abstract: Breast cancer is amongst the most common cancers causing death of women worldwide. Breast cancer occurrence is more prominent in people with diabetes. A recent trend is management of diabetes and cancer has evolved to be natural remedy including single molecule therapy or combination. In this study, we investigated the effect of inotodiol on breast cancer growth in diabetic conditions. Inotodiol is a lanostane triterpenoid found in natural resources like edible mushroom Inonotus obliquus. We established a rat model of diabetic-breast cancer by treating female Sprague-Dawley rats with streptazotocin (STZ) at 35mg/kg followed by induction of breast cancer by administration of 7,12-dimethylbenz(a)anthracene (DMBA) at 10mg/kg. Diabetes development in experimental rats was confirmed by measuring fasting blood glucose levels and oral glucose tolerance test (OGTT), and other biochemical assays were performed. Histological evaluation of pancreas was performed. The proliferation of breast tumor was measured by immunohistochemical staining for PCNA, cleaved-caspase-3 and TUNEL staining for apoptosis, and -catenin. Results of the study demonstrate that inotodiol lowered the blood glucose levels in SDrats as well as reduced plasma levels of cholesterol, triglyceride, and high-density lipoprotein. The tumor proliferation marker PCNA was reduced by inotodiol. It downregulated the expression of -catenin and its downstream targets (c-Myc and Cyclin D1) followed by apoptosis induction. Conclusively, results suggest that inotodiol regulates blood glucose levels in diabetic rats and then controls proliferation of breast tumor progression by inducing apoptosis via downregulation of -catenin signaling. It further suggests that inotodiol can be a preventive approach in managing dietary chronic conditions like diabetic-breast cancer.
PMID: Int J Med Mushrooms. 2017 ;19(6):485-497. PMID: 29199559 Abstract Title: Growth-Inhibitory and Immunomodulatory Activities of Wild Mushrooms from North-Central British Columbia (Canada). Abstract: Wild mushrooms, especially from North America, have not been systematically explored for their medicinal properties. Here we report screening for the growth-inhibitory and immunomodulatory activities of 12 species collected from multiple locations in north-central British Columbia, Canada. Mushrooms were characterized using morphology and DNA sequencing, followed by chemical extraction into 4 fractions using 80% ethanol, 50% methanol, water, and 5% sodium hydroxide. Growth-inhibitory, immunostimulatory, and anti-inflammatory activities of 5 mushrooms (Leucocybe connata, Trichaptum abietinum, Hydnellum sp., Gyromitra esculenta, and Hericium coralloides) are reported here, to our knowledge for the first time. Growth-inhibitory effects were assessed using the cytotoxic MTT assay. Immunostimulatory activity was assessed by tumor necrosis factor- production in Raw 264.7 macrophages, whereas anti-inflammatory activity was assessed based on the inhibition of lipopolysaccharide-induced tumor necrosis factor- production. The ethanol and aqueous extracts of Hydnellum sp. were potent growth inhibitors, with a half-maximal inhibitory concentration of 0.6 mg/mL. All 5 fungi displayed strong immunostimulatory activity, whereas only L. connata and T. abietinum showed strong anti-inflammatory activity. For the 7 other fungi investigated, which included well-known medicinal species such as Inonotus obliquus, Phellinus igniarius, and Ganodermaapplanatum, the remarkable similarities in the biological activities reported here, and by others for specimens collected elsewhere, suggest that mushrooms can produce similar metabolites regardless of their habitat or ecosystem. This is to our knowledge the first study to explore wild mushrooms from British Columbia for biological activities that are relevant to cancer, and the results provide an initial framework for the selection of mushroom species with the potential for discovery of novel anticancer compounds.
Merck's aggressive agenda to increase HPV vaccine uptake rates, despite causing thousands of severe injuries, is hitting a stumbling block in a court case alleging blatant corruption.
There has been documented evidence that the HPV vaccine has caused more injuries than any other vaccination in history. Despite this evidence however, the HPV vaccination has continued to be hailed a success by the pharmaceutical industry and governments alike.
PMID: Int J Med Mushrooms. 2018 ;20(1):1-11. PMID: 29604909 Abstract Title: Antiproliferative Activity and Cytotoxicity of Some Medicinal Wood-Destroying Mushrooms from Russia. Abstract: We analyzed the antiproliferative activity of 6 medicinal wood-destroying mushrooms (Fomes fomentarius, Fomitopsis pinicola, Trametes versicolor, Trichaptum biforme, Inonotus obliquus, and Coniophora puteana) that are common in deciduous and mixed coniferous forests in Central Russia. Morphological identification of strains collected from the wild was confirmed based on ribosomal DNA internal transcribed spacer phylogenetic analysis. We observed cytotoxic and cell growth-inhibitory effects of hot water extracts from mycelial biomass of 5 species-T. versicolor, C. puteana, F. fomentarius, F. pinicola, and I. obliquus-on leukemia cell lines (Jukart, K562, and THP-1); the effective extract concentrations were mostly less than 50g mL-1. However, we observed no antiproliferative activity of dry biomass from methanol-chloroform (1:1) extracts of C. puteana and F. fomentarius. A chemosensitivity assay showed that the most effective polypore mushroom extract was the methanol extract of T. versicolor (strain It-1), which inhibited the growth of 6 various solid tumors (A-549 and SWi573 [lung], HBL-100 and T-47D [breast], HeLa [cervix], and WiDr [colon]) at concentrations below 45 g mL-1, with a concentration as low as 0.7-3.6 g mL-1 causing 50% reduction in the proliferation of cancer cells in lung and cervixtumors. Methanol extracts of F. pinicola and I. obliquus were less effective, with proliferation-inhibiting capacities at concentrations below 70 and 200 g mL-1, respectively. Thus, T. versicolor is a prospective candidate in the search for and production of new antiproliferative chemical compounds.
PMID: Biochem Biophys Res Commun. 2018 Apr 13. Epub 2018 Apr 13. PMID: 29660337 Abstract Title: Effect of betulinic acid and its ionic derivatives on M-MuLV replication. Abstract: Murine leukemia virus (MuLV) is a retrovirus known causing leukemia and neurological disorders in mice, and its viral life cycle and pathogenesis have been investigated extensively over the past decades. As a natural antiviral agent, betulinic acid is a pentacyclic triterpenoid that can be found in the bark of several species of plants (particularly the white birch). One of the hurdles for betulinic acid to release its antiviral potency is its poor water solubility. In this study, we synthesized more water-soluble ionic derivatives of betulinic acid, and examined their activities against Moloney MuLV (M-MuLV). The mouse fibroblast cells stably infected with M-MuLV, 43D cells, were treated with various doses of betulinic acids and its derivatives, and the viral structural protein Gag in cells and media were detected by western blots. Two ionic derivatives containing the benzalkonium cation were found to inhibit the virus production into media and decreased Gag in cells. However, a cell proliferation assay showed that the benzalkonium cation inhibited the growth of 43D cells, suggesting that our ionic derivatives limited virus production through the inhibition of metabolism in 43D cells. Interestingly, all of these betulinic acid compounds exhibited a minimum impact on the processing and release of Gag from 43D cells, which outlines the differences of viral maturation between MuLV and human immunodeficiency virus.
PMID: Integr Cancer Ther. 2018 Feb 1:1534735418757912. Epub 2018 Feb 1. PMID: 29484963 Abstract Title: Chaga ( Inonotus obliquus), a Future Potential Medicinal Fungus in Oncology? A Chemical Study and a Comparison of the Cytotoxicity Against Human Lung Adenocarcinoma Cells (A549) and Human Bronchial Epithelial Cells (BEAS-2B). Abstract: BACKGROUND: Inonotus obliquus, also known as Chaga, is a parasitic fungus growing on birches and used in traditional medicine (especially by Khanty people) to treat various health problems. In this study, we aimed to quantify the 3 metabolites frequently cited in literature, that is, betulin, betulinic acid, and inotodiol in the Chaga recently discovered in forests located in Normandy (France), and to compare their concentrations with Ukrainian and Canadian Chaga. This study also explores the cytotoxicity of the French Chaga against cancer-derived cells and transformed cells.METHODS: A quantification method by HPLC-MS-MS (high-performance liquid chromatography-tandem mass spectrometry) of betulin, betulinic acid, and inotodiol was developed to study the French Chaga and compare the concentration of these metabolites with extracts provided from Chaga growing in Canada and Ukraine. This method was also used to identify and quantify those 3 compounds in other traditional preparations of Chaga (aqueous extract, infusion, and decoction). Among these preparations, the aqueous extract that contains betulin, betulinic acid, and inotodiol was chosen to evaluate and compare its cytotoxic activity toward human lung adenocarcinoma cells (A549 line) and human bronchial epithelial cells (BEAS-2B line).RESULTS: French Chaga contains betulin and betulinic acid at higher levels than in other Chaga, whereas the concentration of inotodiol is greater in the Canadian Chaga. Moreover, the results highlighted a cytotoxic activity of the Chaga's aqueous extract after 48 and 72 hours of exposure with a higher effect on cancer-derived cells A549 than on normal transformed cells BEAS-2B ( P = 0.025 after 48 hours of exposure and P = 0.004 after 72 hours of exposure).
PMID: Toxicol Ind Health. 2018 Jan 1:748233718757082. Epub 2018 Jan 1. PMID: 29656705 Abstract Title: Suppressive effects of neonatal bisphenol A on the neuroendocrine system. Abstract: The aim of this study was to assess the effects of neonatal bisphenol A (BPA) administration on neuroendocrine features (the thyroid-brain axis). BPA (20 or 40g/kg) was orally administered to juvenile male albino rats ( Rattus norvegicus) from postnatal days (PNDs) 15 to 30. Both doses resulted in lower serum thyroxine (T4), triiodothyronine (T3), and growth hormone levels and higher thyrotropin level than the control levels at PND 30. In the neonatal cerebellum and cerebrum, vacuolation, pyknosis, edema, degenerative changes, and reductions in the size and number of the cells were observed in both treated groups. Alternatively, elevations in oxidative markers (lipid peroxidation, nitric oxide, and hydrogen peroxide [HO]) at both dose levels were recorded at PND 30, along with decreased activities of antioxidant markers (ascorbic acid, total thiol [t-SH], glutathione, glutathione peroxidase, glutathione reductase, glutathione-S-transferase, and catalase) with respect to control levels. Thus, the BPA-induced hypothyroid state may disturb the neonatal thyroid-brain axis via production of free radicals, and this could damage the plasma membrane and cellular components, delaying cerebrum and cerebellum development.
Dr Ron whio is a Fellow of the American Academy of Family Practice and a Charter member of the American Academy of Emergency Medicine and a licinsed Acupucturist is joined by Dr Gerry and Dr Dan to host this weeky podcast on the latest medical news and information We are not influenced by Big Pharma and will give you honest opinions on the news, medical devices and we have teriffic guests. We have over 150 years of combined medical experience and are grateful to share it with you. You can hear us on blogtalkradio, stitcher, iTunes, GooglePlay, Alexa and others. Reach us live at 347-989-8899 Dr. Talmor graduated from Hadassah Medical school in Jerusalem in 1974 and now practices holistic medicine in Gainesville, Florida. He is board certified in Pediatrics and board eligible in Emergency Medicine. ? Dr. Talmor has utilized body-mind techniques in his medical practice as a pediatrician and as an emergency room physician since 1976. He taught personal growth classes to inmates in the Florida prison system from 1985 to 1991. He worked extensively and instructed individuals and groups in the use of consciousness and personal growth in self healing.
PMID: Bioessays. 2003 Oct ;25(10):961-70. PMID: 14505363 Abstract Title: Apical meristems: the plant's fountain of youth. Abstract: During postembryonic development, all organs of a plant are ultimately derived from a few pluripotent stem cells found in specialized structures called apical meristems. Here we discuss our current knowledge about the regulation of plant stem cells and their environments with main emphasis on the shoot apical meristem of Arabidopsis thaliana. Recent studies suggest that stem cells are localized in specialized niches where signals from surrounding cells maintain their undifferentiated state. In the shoot meristem, initiation of stem cells during embryogenesis, regulation of stem-cell homeostasis and termination of stem-cell maintenance during flower development appear to primarily involve regulation of the stem-cell niche.
PMID: Toxicon. 2018 Apr 11. Epub 2018 Apr 11. PMID: 29654868 Abstract Title: Bee venom therapy: Potential mechanisms and therapeutic applications. Abstract: Bee venom is a very complex mixture of natural products extracted from honey bee which contains various pharmaceutical properties such as peptides, enzymes, biologically active amines and nonpeptide components. The use of bee venom into the specific points is so called bee venom therapy, which is widely used as a complementary and alternative therapy for 3000 years. A growing number of evidence has demonstrated the anti-inflammation, the anti-apoptosis, the anti-fibrosis and the anti-arthrosclerosis effects of bee venom therapy. With these pharmaceutical characteristics, bee venom therapy has also been used as the therapeutic method in treating rheumatoid arthritis, amyotrophic lateral sclerosis, Parkinson's disease, Alzheimer's disease, liver fibrosis, atherosclerosis, pain and others. Although widely used, several cases still reported that bee venom therapy might cause some adverse effects, such as local itching or swelling. In this review, we summarize its potential mechanisms, therapeutic applications, and discuss its existing problems.
PMID: Cell. 2008 Feb 22 ;132(4):553-7. PMID: 18295573 Abstract Title: Plant stem cell niches: standing the test of time. Abstract: Similar to animal stem cells, plant stem cells require special niche microenvironments to continuously generate the tissues that constitute the plant body. Recent work using computer modeling and live imaging is helping to elucidate some of the mechanisms responsible for the specification and maintenance of stem cells in the root and shoot.
PMID: Curr Opin Plant Biol. 2011 Feb ;14(1):4-9. Epub 2010 Aug 23. PMID: 20739214 Abstract Title: Plant stem cell niches: from signalling to execution. Abstract: The shoot and root meristems contain small populations of stem cells that constantly renew themselves while providing precursor cells to build all other plant tissues and organs. Cell renewal, growth and differentiation in the meristems are co-ordinated by networks of transcription factors and intercellular signals. The past two years have revealed how auxin and cytokinin signals are integrated with each other and with regulatory genes in the shoot and root meristems. Small RNAs have also emerged as novel intercellular signals. Downstream of meristem regulatory genes, links have been made to cell division control and chromatin function. Protection of genome integrity, partly through programmed cell death after DNA damage, has recently been revealed as a specialised function in plant stem cells.
PMID: Biochem Biophys Res Commun. 2011 Jun 10 ;409(3):363-6. Epub 2011 May 6. PMID: 21570951 Abstract Title: Sculpting the meristem: the roles of miRNAs in plant stem cells. Abstract: The whole structure of higher plants is generated dynamically throughout the life cycle by the activity of stem cell niches at the apex of shoot and root. Hormone molecules and many transcription factors cooperate to balance the stem cell maintenance and differentiation. It is becoming increasingly clear that microRNA (miRNA) molecules are also participants in these processes. Here, we highlight the advances that have been made in regarding the roles of miRNAs in plant stem cell control. These advances provide a framework for our understanding of how signals are integrated to specify and position the stem cell niches in plants.
PMID: Curr Opin Plant Biol. 2012 Dec ;15(6):652-8. Epub 2012 Jun 21. PMID: 22727764 Abstract Title: Argonautes compete for miR165/166 to regulate shoot apical meristem development. Abstract: Plant stem cells in the shoot apical meristem (SAM) possess the unique abilities of both self-renewal for SAM maintenance and providing undifferentiated daughter cells for initiation and subsequent development of aerial organs. The coordination between stem cell renewal and cell differentiation during organogenesis is regulated by elaborate genetic pathways involving numerous transcription factors and other molecules. In the past decade, microRNAs (miRNAs) have emerged as pivotal regulators in many biological processes including meristem homeostasis and differentiation in plants. In this review, we summarize current knowledge about the function and mechanism of a family of miRNAs (miR165/166), the miRNA-designated Argonautes (AGOs), and the miRNA-regulated targets in SAM development and maintenance.
PMID: J Exp Bot. 2013 Mar ;64(5):1153-65. Epub 2012 Nov 25. PMID: 23183258 Abstract Title: Hormonal regulation of stem cell maintenance in roots. Abstract: During plant embryogenesis, the apical-basal axis is established and both the shoot apical meristem (SAM) and the root apical meristem (RAM) are formed. In both meristems, there are slowly dividing cells which control the differentiation of their surrounding cells called the organizing centre (OC) and the quiescent centre (QC) in the shoot and root, respectively. These centres with their surrounding initial cells form a 'stem cell niche'. The initial cells eventually differentiate into various plant tissues, giving rise to plant organs such as lateral shoots, flowers, leaves, and lateral roots. Plant hormones are important factors involved in the balance between cell division and differentiation such that plant growth and development are tightly controlled in space and time. No single hormone acts by itself in regulating the meristematic activity in the root meristem. Division and differentiation are controlled by interactions between several hormones. Intensive research on plant stem cells has focused on how cell division is regulated to form specific plant organs and tissues, how differentiation is controlled, and how stem cell fate is coordinated. In this review, recent knowledge pertaining to the role of plant hormones in maintaining root stem cells including the QC is summarized and discussed. Furthermore, we suggest diverse approaches to answering the main question of how root stem cells are regulated and maintained by plant hormones.
PMID: Trends Cell Biol. 2014 Aug ;24(8):443-8. Epub 2014 Apr 3. PMID: 24704103 Abstract Title: A quiescent path to plant longevity. Abstract: The giant sequoia and the bristlecone pine trees are capable of living up to several hundreds or even thousands of years. Plants achieve this longevity by regenerating stem cells capable of giving rise to all differentiated cells. Plant stem cells reside in specific niches with high mitotic activity that are known as meristems. Remarkably, at the center of the root stem cell niche (SCN) resides a group of mitotically inactive cells known as the quiescent center (QC). Recent studies suggest that stress-related phytohormones and DNA damage can initiate QC cell division, resulting in the replenishment of stem cells surrounding the QC. We therefore propose that the QC represents a pool of backup cells that serve to replace damaged stem cells, thereby contributing to plant longevity.
PMID: Nat Rev Mol Cell Biol. 2014 May ;15(5):301-12. PMID: 24755933 Abstract Title: Plant and animal stem cells: similar yet different. Abstract: The astonishingly long lives of plants and their regeneration capacity depend on the activity of plant stem cells. As in animals, stem cells reside in stem cell niches, which produce signals that regulate the balance between self-renewal and the generation of daughter cells that differentiate into new tissues. Plant stem cell niches are located within the meristems, which are organized structures that are responsible for most post-embryonic development. The continuous organ production that is characteristic of plant growth requires a robust regulatory network to keep the balance between pluripotent stem cells and differentiating progeny. Components of this network have now been elucidated and provide a unique opportunity for comparing strategies that were developed in the animal and plant kingdoms, which underlie the logic of stem cell behaviour.
PMID: Pharmacogn Rev. 2014 Jul ;8(16):105-12. PMID: 25125883 Abstract Title: Molecule mechanism of stem cells in Arabidopsis thaliana. Abstract: Plants possess the ability to continually produce new tissues and organs throughout their life. Unlike animals, plants are exposed to extreme variations in environmental conditions over the course of their lives. The vitality of plants is so powerful that they can survive several hundreds of years or even more making it an amazing miracle that comes from plant stem cells. The stem cells continue to divide to renew themselves and provide cells for the formation of leaves, stems, and flowers. Stem cells are not only quiescent but also immortal, pluripotent and homeostatic. Stem cells are the magic cells that repair tissues and regenerate organs. During the past decade, scholars around the world have paid more and more attention toward plant stem cells. At present, the major challenge is in relating molecule action mechanism to root apical meristem, shoot apical meristem and vascular system. The coordination between stem cells maintenance and differentiation is critical for normal plant growth and development. Elements such as phytohormones, transcription factors and some other known or unknown genes cooperate to balance this process. In this review, Arabidopsis thaliana as a pioneer system, we highlight recent developments in molecule modulating, illustrating how plant stem cells generate new mechanistic insights into the regulation of plants growth and development.
PMID: Zhongguo Zhong Yao Za Zhi. 2014 Jan ;39(2):343-5. PMID: 24761658 Abstract Title: [On plant stem cells and animal stem cells]. Abstract: A comparison of plant and animal stem cells can highlight core aspects of stem-cell biology. In both kingdoms, stem cells are defined by their clonogenic properties and are maintained by intercellular signals. The signaling molecules are different in plants and animals stem cell niches, but the roles of argonaute and polycomb group proteins suggest that there are some molecular similarities.
Pesticides cause a multitude of adverse effects on humans. However, they are especially harmful to children. They may have something to do with the mass-shootings in schools all over America because some of them are neurotoxins. This means they affect and damage the central nervous system and the brain of all animals, including humans.
Warren Porter, professor emeritus of zoology at the University of Wisconsin, conducted experiments with ground water water drawn from the ground of farms with typical levels of pesticides and fertilizers. He chose farm water contaminated with the insecticide aldicarb, the herbicide atrazine, and nitrogen fertilizer. He tested that mixture on white mice and deer mice.
The concentrations of the fertilizer with each of the pesticides (aldicarb and atrazine) in the ground water were of the order of magnitude the Environmental Protection Agency says the chemicals cause no unreasonable harm to man and the environment....
Could a diet high in refined sugars make children and adults more susceptible to opioid addiction and overdose? New research, from our laboratory of behavioral neuroscience at the University of Guelph, suggests it could.
Approximately 20,000 people died of fentanyl-related overdoses in the United States last year and in Canada there were at least 2,816 opioid-related deaths. During 2017 so far, over 1,000 people have died of illicit drug overdoses in British Columbia. High schools are stocking up on the overdose-reversing drug naloxone and universities are training staff to administer the drug.
Nobody is talking about sugar.
And yet there is substantial experimental evidence that refined sugar can promote addictive behaviours by activating the brains rewards centres in much the same way as addictive drugs. Opioid abuse is also associated with poor dietary habits, including preferences for sugar-rich foods, as well as malnutrition. These connections have led to questions of whether excessive consumption of refined sugar may affect vulnerability to opioid addiction....
by Health Impact News/MedicalKidnap.com Staff
Tara Chapman, a 33 year old disabled mother, was handcuffed, drugged, and carted away to a facility that is 8 hours from her family. She is being held prisoner in Tennessee under the guise of mental illness, even though a mental hospital in Virginia recently said that she did not meet the criteria to commit her.
The state of Tennessee wants Taras three boys to stay in foster care, away from family. The family wants to know how far the state is willing to go to ensure that neither Tara nor her family gets her 3 children back? A fourth child, Taras daughter, is being cared for by her mother Whitney, the childrens grandmother, but the state refuses to allow the grandmother to care for the 3 boys.
There was supposed to be a court hearing on Friday, April 13, 2018, but the juvenile court cancelled it without warning less than 24 hours before.
Tara has suffered from mild mental illness for many years, but it was only after her children were seized by Child Protective Services and abused in foster care that her mental health deteriorated to the point where she qualified for disability.
See original story, reported in January:
She was recently home with family members in Virginia for almost 3 weeks before she was medically kidnapped. During that time, her mother Whitney Manning was able to ween her off of the psychotropic medications.
The change in Tara was almost miraculous. Whitney found that the symptoms in her daughter that they had been led to believe were due to her mental illness disappeared. She stopped hallucinating. The crazy tal...
PMID: Acta Pol Pharm. 2014 Sep-Oct;71(5):701-7. PMID: 25362798 Abstract Title: Plant stem cells as innovation in cosmetics. Abstract: The stem cells thanks to their ability of unlimited division number or transformation into different cell types creating organs, are responsible for regeneration processes. Depending on the organism in which the stem cells exists, they divide to the plant or animal ones. The later group includes the stem cells existing in both embryo's and adult human's organs. It includes, among others, epidermal stem cells, located in the hair follicle relieves and also in its basal layers, and responsible for permanent regeneration of the epidermis. Temporary science looks for method suitable for stimulation of the epidermis stem cells, amongst the other by delivery of e.g., growth factors for proliferation that decrease with the age. One of the methods is the use of the plant cell culture technology, including a number of methods that should ensure growth of plant cells, issues or organs in the environment with the microorganism-free medium. It uses abilities of the different plant cells to dedifferentiation into stem cells and coming back to the pluripotent status. The extracts obtained this way from the plant stem cells are currently used for production of both common or professional care cosmetics. This work describes exactly impact of the plant stem cell extract, coming from one type of the common apple tree (Uttwiler Sptlauber) to human skin as one of the first plant sorts, which are used in cosmetology and esthetic dermatology.
PMID: Nutr Res Pract. 2018 Apr ;12(2):101-109. Epub 2018 Mar 22. PMID: 29629026 Abstract Title: Effect of vitamin C on azoxymethane (AOM)/dextran sulfate sodium (DSS)-induced colitis-associated early colon cancer in mice. Abstract: BACKGROUND/OBJECTIVES: The objective of this study was to investigate the effects of vitamin C on inflammation, tumor development, and dysbiosis of intestinal microbiota in an azoxymethane (AOM)/dextran sulfate sodium (DSS)-induced inflammation-associated early colon cancer mouse model.MATERIALS/METHODS: Male BALB/c mice were injected intraperitoneally with AOM [10 mg/kg body weight (b.w)] and given two 7-d cycles of 2% DSS drinking water with a 14 d inter-cycle interval. Vitamin C (60 mg/kg b.w. and 120 mg/kg b.w.) was supplemented by gavage for 5 weeks starting 2 d after the AOM injection.RESULTS: The vitamin C treatment suppressed inflammatory morbidity, as reflected by disease activity index (DAI) in recovery phase and inhibited shortening of the colon, and reduced histological damage. In addition, vitamin C supplementation suppressed mRNA levels of pro-inflammatory mediators and cytokines, including cyclooxygenase-2, microsomal prostaglandin E synthase-2, tumor necrosis factor-, Interleukin, and, and reduced expression of the proliferation marker, proliferating cell nuclear antigen, compared to observations of AOM/DSS animals. Although the microbial composition did not differ significantly between the groups, administration of vitamin C improved the level of inflammation-relatedandto control levels.CONCLUSION: Vitamin C treatment provided moderate suppression of inflammation, proliferation, and certain inflammation-related dysbiosis in a murine model of colitis associated-early colon cancer. These findings support that vitamin C supplementation can benefit colonic health. Long-term clinical studies with various doses of vitamin C are warranted.
PMID: Front Plant Sci. 2015 ;6:1028. Epub 2015 Nov 18. PMID: 26635851 Abstract Title: Involvement of Plant Stem Cells or Stem Cell-Like Cells in Dedifferentiation. Abstract: Dedifferentiation is the transformation of cells from a given differentiated state to a less differentiated or stem cell-like state. Stem cell-related genes play important roles in dedifferentiation, which exhibits similar histone modification and DNA methylation features to stem cell maintenance. Hence, stem cell-related factors possibly synergistically function to provide a specific niche beneficial to dedifferentiation. During callus formation in Arabidopsis petioles, cells adjacent to procambium cells (stem cell-like cells) are dedifferentiated and survive more easily than other cell types. This finding indicates that stem cells or stem cell-like cells may influence the dedifferentiating niche. In this paper, we provide a brief overview of stem cell maintenance and dedifferentiation regulation. We also summarize current knowledge of genetic and epigenetic mechanisms underlying the balance between differentiation and dedifferentiation. Furthermore, we discuss the correlation of stem cells or stem cell-like cells with dedifferentiation.
PMID: J Ginseng Res. 2015 Oct ;39(4):376-83. Epub 2015 Apr 30. PMID: 26869831 Abstract Title: Protective effect of wild ginseng cambial meristematic cells on d-galactosamine-induced hepatotoxicity in rats. Abstract: BACKGROUND: Panax ginseng has a wide range of biological activities including anti-inflammatory, antioxidant, and immunomodulatory functions. Wild ginseng cambial meristematic cells (CMCs) were obtained from P. ginseng cambium. This study examined the protective mechanism of wild ginseng CMCs against d-galactosamine (GalN)-induced liver injury. GalN, a well-known hepatotoxicant, causes severe hepatocellular inflammatory damage and clinical features similar to those of human viral hepatitis in experimental animals.METHODS: Hepatotoxicity was induced in rats using GalN (700 mg/kg, i.p.). Wild ginseng CMCs was administered orally once a day for 2 wks, and then 2 h prior to and 6 h after GalN injection.RESULTS: Wild ginseng CMCs attenuated the increase in serum aminotransferase activity that occurs 24 h after GalN injection. Wild ginseng CMCs also attenuated the GalN-induced increase in serum tumor necrosis factor-, interleukin-6 level, and hepatic cyclooxygenase-2 protein and mRNA expression. Wild ginseng CMCs augmented the increase in serum interleukin -10 and hepatic heme oxygenase-1 protein and mRNA expression that was induced by GalN, inhibited the increase in the nuclear level of nuclear factor-kappaB, and enhanced the increase in NF-E2-related factor 2.CONCLUSION: Our findings suggest that wild ginseng CMCs protects liver against GalN-induced inflammation by suppressing proinflammatory mediators and enhancing production of anti-inflammatory mediators.
PMID: Sci Adv. 2016 Jan ;2(1):e1500989. Epub 2016 Jan 29. PMID: 27152324 Abstract Title: An epidermis-driven mechanism positions and scales stem cell niches in plants. Abstract: How molecular patterning scales to organ size is highly debated in developmental biology. We explore this question for the characteristic gene expression domains of the plant stem cell niche residing in the shoot apical meristem. We show that a combination of signals originating from the epidermal cell layer can correctly pattern the key gene expression domains and notably leads to adaptive scaling of these domains to the size of the tissue. Using live imaging, we experimentally confirm this prediction. The identified mechanism is also sufficient to explain de novo stem cell niches in emerging flowers. Our findings suggest that the deformation of the tissue transposes meristem geometry into an instructive scaling and positional input for the apical plant stem cell niche.
PMID: Sci China Life Sci. 2016 Aug ;59(8):825-31. Epub 2016 Aug 10. PMID: 27502904 Abstract Title: Recent advances in the research for the homolog of breast cancer associated gene AtROW1 in higher plants. Abstract: BARD1 (BRCA1 associated RING domain protein 1), as an important animal tumor suppressor gene associated with many kinds of cancers, has been intensively studied for decades. Surprisingly, homolog of BARD1 was found in plants and it was renamed AtROW1 (repressor of Wuschel-1) according to its extremely important function with regard to plant stem cell homeostasis. Although great advances have been made in human BARD1, the function of this animal tumor-suppressor like gene in plant is not well studied and need to be further elucidated. Here, we review and summarize past and present work regarding this protein. Apart from its previously proposed role in DNA repair, recently it is found essential for shoot and root stem cell development and differentiation in plants. The study of AtROW1 in plant may provide an ideal model for further elucidating the functional mechanism of BARD1 in mammals.
PMID: Curr Biol. 2016 09 12 ;26(17):R816-21. PMID: 27623267 Abstract Title: Plant Stem Cells. Abstract: Among the trending topics in the life sciences, stem cells have received a fair share of attention in the public debate - mostly in connection with their potential for biomedical application and therapies. While the promise of organ regeneration and the end of cancer have captured our imagination, it has gone almost unnoticed that plant stem cells represent the ultimate origin of much of the food we eat, the oxygen we breathe, as well the fuels we burn. Thus, plant stem cells may be ranked among the most important cells for human well-being. Research by many labs in the last decades has uncovered a set of independent stem cell systems that fulfill the specialized needs of plant development and growth in four dimensions. Surprisingly, the cellular and molecular design of these systems is remarkably similar, even across diverse species. In some long-lived plants, such as trees, plant stem cells remain active over hundreds or even thousands of years, revealing the exquisite precision in the underlying control of proliferation, self-renewal and differentiation. In this minireview, we introduce the basic features of the three major plant stem cell systems building on these facts, highlight their modular design at the level of cellular layout and regulatory underpinnings and briefly compare them with their animal counterparts.
PMID: EMBO J. 2017 10 2 ;36(19):2844-2855. Epub 2017 Aug 24. PMID: 28838936 Abstract Title: Redox regulation of plant stem cell fate. Abstract: Despite the importance of stem cells in plant and animal development, the common mechanisms of stem cell maintenance in both systems have remained elusive. Recently, the importance of hydrogen peroxide (HO) signaling in priming stem cell differentiation has been extensively studied in animals. Here, we show that different forms of reactive oxygen species (ROS) have antagonistic roles in plant stem cell regulation, which were established by distinct spatiotemporal patterns of ROS-metabolizing enzymes. The superoxide anion (O2-) is markedly enriched in stem cells to activateand maintain stemness, whereas HOis more abundant in the differentiating peripheral zone to promote stem cell differentiation. Moreover, HOnegatively regulates O2- biosynthesis in stem cells, and increasing HOlevels or scavenging O2- leads to the termination of stem cells. Our results provide a mechanistic framework for ROS-mediated control of plant stem cell fate and demonstrate that the balance between O2- and HOis key to stem cell maintenance and differentiation.
PMID: Future Sci OA. 2017 Nov ;3(4):FSO226. Epub 2017 Jul 12. PMID: 29134115 Abstract Title: Plant stem cells in cosmetics: current trends and future directions. Abstract: Plant regeneration at the cellular and tissue level is a unique process. Similar to animals, the stem cells in plants have properties that help stimulate and regenerate plants after injury. The unique properties of plant stem cells have been a recent area of interest and focus both in developing new cosmetics and studying how these extracts/phytohormones will influence animal skin. This special report focuses on the current evidence-based trends in plant stem cell-based cosmetics and sheds light on the challenges that we need to overcome in order to see meaningful changes in human skin using topical cosmetics derived from plant stem cells.
by Health Impact News/MedicalKidnap.com Staff
Tara Chapman, a 33 year old disabled mother, was handcuffed, drugged, and carted away to a facility that is 8 hours from her family. She is being held prisoner in Tennessee under the guise of mental illness, even though a mental hospital in Virginia recently said that she did not meet the criteria to commit her.
The state of Tennessee wants Taras three boys to stay in foster care, away from family. The family wants to know how far the state is willing to go to ensure that neither Tara nor her family gets her 3 children back? A fourth child, Taras daughter, is being cared for by her mother Whitney, the childrens grandmother, but the state refuses to allow the grandmother to care for the 3 boys.
There was supposed to be a court hearing on Friday, April 13, 2018, but the juvenile court cancelled it without warning less than 24 hours before.
Tara has suffered from mild mental illness for many years, but it was only after her children were seized by Child Protective Services and abused in foster care that her mental health deteriorated to the point where she qualified for disability.
See original story, reported in January:
She was recently home with family members in Virginia for almost 3 weeks before she was medically kidnapped. During that time, her mother Whitney Manning was able to ween her off of the psychotropic medications.
The change in Tara was almost miraculous. Whitney found that the symptoms in her daughter that they had been led to believe were due to her mental illness disappeared. She stopped hallucinating. The crazy talk c...
PMID: J Biol Regul Homeost Agents. 2014 Jan-Mar;28(1):141-5. PMID: 24750800 Abstract Title: White mulberry supplementation as adjuvant treatment of obesity. Abstract: Body weight is controlled by our genes and managed by a neuro-hormonal system, in particular by insulin and glucagon. The meristematic extract of Japanese white mulberry blocks the alpha-glucosidase and then the intestinal hydrolysis of polysaccharides, thereby reducing the glycaemic index of carbohydrates. The target of our research was to evaluate the adjuvant slimming effect of the extract of white Japanese mulberry in the dietetic treatment of some patients who are obese or overweight. 46 overweight people were enrolled and divided into two subgroups: the subjects of both subgroups were given an identical balanced diet of 1300 kcal: subjects of the subgroup alpha received 2400 mg of white Japanese mulberry extract, the subgroup b subjects receive placebo. Each subgroup was followed-up every 30 days at 30, 60 and 90 days of treatment. Both in the periodic inspections and in the final inspection measurements of body weight and waist circumference in all the subjects and thigh circumference in women only were repeated. All subjects repeated blood tests. In the subgroup alpha, weight loss was about 9 kg in 3 months, equal to approximately 10 percent of the initial weight, significantly higher than subgroup beta (P
PMID: Biotechnol Genet Eng Rev. 2012 ;28:47-59. PMID: 22616481 Abstract Title: Plant natural products: history, limitations and the potential of cambial meristematic cells. Abstract: Humans have utilised plant derived natural products as medicines for millenia. Moreover, many contemporary pharmaceuticals are also natural products or derivatives thereof. However, the full potential of these compounds remains to be exploited because often they are: complex and difficult to synthesise; found in low quantities; produced by undomesticated and sometimes rare plants; and, their synthesis is routinely influenced by weather conditions. Potentially, the in vitro culture of cells from the corresponding plant species could circumvent some of these problems but the growth of plant cells on an industrial scale is also problematic. The recent isolation and culture of cambial meristematic cells (CMCs), stem cells which ordinarily generate the plant vasculature, may now provide a key platform technology to help realise the full potential of plant natural products.
PMID: Mycopathologia. 2011 Nov ;172(5):373-80. Epub 2011 Jun 26. PMID: 21706309 Abstract Title: Melanization of a meristematic mutant of Fonsecaea monophora increases tolerance to stress factors while no effects on antifungal susceptibility. Abstract: Melanin is a complex polymer, which is widely distributed in nature, and is known as an important virulence factor in opportunistic and pathogenic fungi. In this study, three melanin mutants of Fonsecaea monophora from a case of chromoblastomycosis were generated from a parent strain that lacked hyphal morphology but was meristematic instead. Two albino mutants, one of which (CBS 125187) produced secreted melanin and another (CBS 125149) lacked melanin, grew faster than a mutant with cell-wall-associated and secreted melanin (CBS 125188) and than the meristematic parent strain (CBS 122845) (P
PMID: Biometals. 2009 Jun ;22(3):479-90. Epub 2009 Jan 19. PMID: 19152114 Abstract Title: Red cabbage anthocyanin extract alleviates copper-induced cytological disturbances in plant meristematic tissue and human lymphocytes. Abstract: Red cabbage is a source of health beneficial substances with antioxidant and antigenotoxic properties. HPLC analysis specifying the content of the investigated extract indicated that mainly anthocyanins (ATH) were responsible for its abilities. Cytological research was conducted with two experimental models: plant tissues--meristematic cells of Vicia faba, and animal tissue elements--human lymphocytes. Positive influence of ATH extract on mitotic activity of Vicia cells exposed to Cu(2+) stress, and inhibitory effect of ATH on cytotoxic actions of Cu(2+) on lymphocytes were demonstrated. In all experimental series with ATH application in combinations with Cu(2+), mitotic index (MI) were higher than those obtained for only Cu(2+) stressed tissues. Preincubation in ATH before Cu(2+) stress had the best effect. Similarly, after ATH applications in all tested series decrease in frequency of micronuclei (MN) appearance was noticed in comparison with only Cu(2+) stressed material. In the case of Vicia cells ATH acted effectively even applied after Cu(2+) stress. It suggests that this ATH mixture not only prevents and limits but also heals the cytological injury caused by Cu(2+) stress.
This is neither fact
nor expert advice:
I am an artist, a poet, and all that may be in-between.
This is neither fact
nor expert advice:
Specialisms are making nonsense of sense.
We are being divided.
PMID: J Ethnopharmacol. 2008 Feb 28 ;116(1):173-8. Epub 2007 Nov 28. PMID: 18191353 Abstract Title: Inhibitory effects of kratom leaf extract (Mitragyna speciosa Korth.) on the rat gastrointestinal tract. Abstract: Kratom (Mitragyna speciosa Korth.) is an indigenous plant of Thailand used traditionally in folk medicine although it is claimed to cause addiction. It is used to treat diarrhea, however, there is no scientific evidence to support the use. The aim of this study is to investigate the effect of methanolic extract of kratom leaves on the rat gastrointestinal tract. Kratom extract at 50, 100, 200 and 400 mg/kg (p.o.) caused a dose dependent protection against castor oil-induced diarrhea in rats and also inhibited intestinal transit. The antidiarrheal effect was not antagonized by naloxzone. The inhibition of intestinal transit by kratom extract was significantly different from the control when treated with a single dose for 1 day. For longer-term treatments of 15 and 30 days, kratom extract did not decrease the intestinal transit time indicating that adaptation had occurred. Kratom extract at a dose level of 200 and 400 mg/kg for 30 days and morphine at 3 mg/kg (i.p.) caused a decrease in the increment of body weight that was significantly different from the control and kratom extract at lower doses (50 and 100 mg/kg). However it had no effect on the level of plasma cholecystokinin. The results suggested that methanolic kratom extract exhibited its antidiarrheal effect on rat gastrointestinal tract. The effects may occur via pathways in addition to the action on opioid receptors. High does of kratom extract decreased the increment of body weight similar to the effect of morphine.
PMID: Neurosci Biobehav Rev. 2013 Feb ;37(2):138-51. Epub 2012 Dec 1. PMID: 23206666 Abstract Title: From Kratom to mitragynine and its derivatives: physiological and behavioural effects related to use, abuse, and addiction. Abstract: Kratom (or Ketum) is a psychoactive plant preparation used in Southeast Asia. It is derived from the plant Mitragyna speciosa Korth. Kratom as well as its main alkaloid, mitragynine, currently spreads around the world. Thus, addiction potential and adverse health consequences are becoming an important issue for health authorities. Here we reviewed the available evidence and identified future research needs. It was found that mitragynine and M. speciosa preparations are systematically consumed with rather well defined instrumentalization goals, e.g. to enhance tolerance for hard work or as a substitute in the self-treatment of opiate addiction. There is also evidence from experimental animal models supporting analgesic, muscle relaxant, anti-inflammatory as well as strong anorectic effects. In humans, regular consumption may escalate, lead to tolerance and may yield aversive withdrawal effects. Mitragynine and its derivatives actions in the central nervous system involve-opioid receptors, neuronal Ca channels and descending monoaminergic projections. Altogether, available data currently suggest both, a therapeutic as well as an abuse potential.
According to a recent AP investigation, the world of medicine is often far more forgiving about sexual misconduct than it should be. And certainly more forgiving than weve seen in pop culture over the last few months. If a doctor is found to have committed some type of misconduct, when and IF they are disciplined,
PMID: J Psychoactive Drugs. 2015 Apr-Jun;47(2):125-31. PMID: 25950592 Abstract Title: Social Functioning of Kratom (Mitragyna speciosa) Users in Malaysia. Abstract: Kratom (Mitragyna speciosa) is an indigenous plant known for its traditional medicinal use, and for its addiction potential, in Southeast Asia. In recent years, kratom and its major alkaloid, mitragynine, spread worldwide with largely unknown effects on behavior and mental health. Recent studies show that kratom use can lead to dependence and that mitragynine works as an addictive drug in animal studies. Nevertheless, kratom preparations were also suggested as a less harmful substitute in opiate withdrawal. Potential side-effects of prolonged kratom use, however, are currently unclear. The aim of this study was to investigate the social functioning of regular kratom users in Malaysia. A cross-sectional survey was carried out in three northern states of Peninsular Malaysia investigating 293 regular kratom consumers using the Addiction Severity Index in a snowball sampling technique. Findings showed that regular kratom users do not experience major impairments in their social functioning, despite being dependent on kratom for prolonged periods. Our findings suggest that chronic kratom administration does not significantly impair social functioning of users in a natural context in Malaysia.
PMID: Drug Des Devel Ther. 2015 ;9:2421-9. Epub 2015 Apr 29. PMID: 25995615 Abstract Title: Pharmacokinetics of mitragynine in man. Abstract: BACKGROUND: Kratom, known botanically as Mitragyna speciosa (Korth.), is an indigenous tree in Southeast Asia. Kratom is currently easily available worldwide via special shops and the Internet to use as a drug of abuse, opioid alternative, or pain killer. So far, the pharmacokinetics of this plant has been studied only in animals, and there is no such study in humans. The major abundant active alkaloid in Kratom, mitragynine, is one of the promising new chemical substances to be developed as a new drug. The aim of this study was to examine the pharmacokinetics of mitragynine and assess the linearity in pharmacokinetics in chronic users.METHODS: Since Kratom is illegal in Thailand, studies in healthy subjects would be unethical. We therefore conducted a prospective study by enrolling ten chronic, regular, healthy users. We adjusted the steady state in each subject by giving a known amount of Kratom tea for 7 days before commencement of the experiment. We admitted and gave different oral doses to subjects to confirm linearity in pharmacokinetics. The mitragynine blood concentrations at 17 times points and the urine concentrations during the 24-hour period were collected and measured by liquid chromatography-tandem mass spectrometry method.RESULTS: Ten male subjects completed the study without adverse reactions. The median duration of abuse was 1.75 years. We analyzed one subject separately due to the abnormal behavior of blood concentration. From data of nine subjects, the pharmacokinetic parameters established were time to reach the maximum plasma concentration (0.830.35 hour), terminal half-life (23.2416.07 hours), and the apparent volume of distribution (38.0424.32 L/kg). The urine excretion of unchanged form was 0.14%. The pharmacokinetics were observed to be oral two-compartment model.CONCLUSION: This was the first pharmacokinetic study in humans, which demonstrated linearity and was consistent with the oral two-compartment model with a terminal half-life of about 1 day. The pharmacokinetic linearity and parameters reported are necessary pharmacological information of Kratom, and there is a possibility for it to be developed medically as a pain killer or better opioid substitute in the future.
PMID: J Psychoactive Drugs. 2015 Nov-Dec;47(5):360-7. Epub 2015 Nov 23. PMID: 26595229 Abstract Title: Experiences of Kratom Users: A Qualitative Analysis. Abstract: Kratom (Mitragyna speciosa) is a psychoactive plant that has been used since at least 1836 in folk medicine in Southeast Asian countries. More recently, kratom has become widely available in the West and is used for both recreational and medicinal purposes. There has, however, been little scientific research into the short- and long-term effects of kratom in humans, and much of the information available is anecdotal. To supplement the increasing scientific understanding of kratom's pharmacology and research into its effects in animals, we report the results of a qualitative analysis of first-hand descriptions of human kratom use that were submitted to, and published by, a psychoactive substance information website (Erowid.org). Themes that emerged from these experience reports indicate that kratom may be useful for analgesia, mood elevation, anxiety reduction, and may aid opioid withdrawal management. Negative response themes also emerged, indicating potential problems and unfavorable"side"effects, especially stomach upset and vomiting. Based on our analyses, we present preliminary hypotheses for future examination in controlled, quantitative studies of kratom.
n/a PMID: Front Pharmacol. 2017 ;8:96. Epub 2017 Feb 28. PMID: 28293192 Abstract Title: Indole Alkaloids from Plants as Potential Leads for Antidepressant Drugs: A Mini Review. Abstract: Depression is the most common illness observed in the elderly, adults, and children. Antidepressants prescribed are usually synthetic drugs and these can sometimes cause a wide range of unpleasant side effects. Current research is focussed on natural products from plants as they are a rich source of potent new drug leads. Besides(St. John's wort), the plants studied includeL. (passion flower),(kratom),G. Forst (kava) andL. Harman, harmol, harmine, harmalol and harmaline are indole alkaloids isolated from, while mitragynine is isolated from. The structure of isolated compounds fromG. Forst andL. contains an indole moiety. The indole moiety is related to the neurotransmitter serotonin which is widely implicated for brain function and cognition as the endogenous receptor agonist. An imbalance in serotonin levels may influence mood in a way that leads to depression. The moiety is present in a number of antidepressants already on the market. Hence, the objective of this review is to discuss bioactive compounds containing the indole moiety from plants that can serve as potent antidepressants.
PMID: J Ethnopharmacol. 2017 Apr 18 ;202:302-325. Epub 2017 Mar 19. PMID: 28330725 Abstract Title: A botanical, phytochemical and ethnomedicinal review of the genus Mitragyna korth: Implications for products sold as kratom. Abstract: ETHNOPHARMACOLOGICAL RELEVANCE: The genus Mitragyna (Rubiacaeae) has been traditionally used in parts of Africa, Asia and Oceania. In recent years, there has been increased interest in species of Mitragyna with the introduction of products to western markets and regulatory uncertainty.AIM OF THE STUDY: This paper reviewed the traditional ethnomedicinal uses of leaves for species belonging to the genus Mitragyna with reference to the botany and known chemistry in order to highlight areas of interest for products currently being sold as kratom.MATERIALS AND METHODS: A literature search was conducted using Web of Science, Google Scholar, the Royal Museum for Central Africa, Internet Archive, Hathi Trust, and Biodiversity Heritage Library search engines in the spring of 2015, fall of 2016 and winter of 2017 to document uses of bark, leaf and root material.RESULTS: Leaves of M. speciosa (kratom) had the most common documented ethnomedicinal uses as an opium substitute or remedy for addiction. Other species of Mitragyna were reportedly used for treating pain, however the mode of preparation was most often cited as topical application. Other uses of Mitragyna included treatment of fever, skin infections, and as a mild anxiolytic.CONCLUSIONS: Mitragyna species have been used medicinally in various parts of the world and that there is significant traditional evidence of use. Modern products that include formulations as topical application of liniments, balms or tinctures may provide effective alternatives for treatment of certain types of pains. Future research is required to establish safety and toxicology limits, medicinal chemistry parameters and the potential for different physiological responses among varying genetic populations to support regulatory requirements for Mitragyna spp.
PMID: J Ethnopharmacol. 2017 Jun 9 ;205:178-185. Epub 2017 May 10. PMID: 28501425 Abstract Title: The effects of kratom on restraint-stress-induced analgesia and its mechanisms of action. Abstract: ETHNOPHARMACOLOGICAL RELEVANCE: Mitragyna speciosa and its extracts are called kratom (dried leaves, extract). They contain several alkaloids with an affinity for different opioid receptors. They are used in traditional medicine for the treatment of different diseases, as a substitute by opiate addicts, and to mitigate opioid withdrawal symptoms. Apart from their medical properties, they are used to enhance physical endurance and as a means of overcoming stress.PURPOSE: The aim of this study was to determine the mechanisms underlying the effects of kratom on restraint-stress-induced analgesia which occurs during or following exposure to a stressful or fearful stimulus.METHODS: To gain further insights into the action of kratom on stress, we conducted experiments using restraint stress as a test system and stress-induced analgesia as a test parameter. Using transgenic mu opioid-receptor (MOR) deficient mice, we studied the involvement of this receptor type. We used nor-binaltorphimine (BNT), an antagonist at kappa opioid receptors (KOR), to study functions of this type of receptor. Membrane potential assay was also employed to measure the intrinsic activity of kratom in comparison to U50,488, a highly selective kappa agonist.RESULTS: Treatment with kratom diminished stress-induced analgesia in wildtype and MOR knockout animals. Pretreatment of MOR deficient mice with BNT resulted in similar effects. In comparison to U50,488, kratom exhibited negligible intrinsic activity at KOR alone.CONCLUSIONS: The results suggest that the use of kratom as a pharmacological tool to mitigate withdrawal symptoms is related to its action on KOR.
PMID: Drug Alcohol Depend. 2018 Feb 1 ;183:134-140. Epub 2017 Dec 7. PMID: 29248691 Abstract Title: Kratom use and mental health: A systematic review. Abstract: BACKGROUND: Kratom (Mitragyna speciosa) is a psychoactive plant native to Southeastern Asia that is receiving increased international attention as a potential therapeutic agent. While much of the limited scientific research on kratom is focused on its analgesic potential, kratom use also has important risks and benefits in the domain of mental health.METHODS: We conducted a comprehensive systematic review of all studies on kratom use and mental health published between January 1960 and July 2017.RESULTS: Findings indicate kratom's potential as a harm reduction tool, most notably as a substitute for opioids among people who are addicted. Kratom also enhances mood and relieves anxiety among many users. For many, kratom's negative mental health effects - primarily withdrawal symptoms - appear to be mild relative to those of opioids. For some users, however, withdrawal is highly uncomfortable and maintaining abstinence becomes difficult.CONCLUSION: Results inform clinicians working in the mental health and substance use fields, policy-makers, and researchers about the mental health effects of this plant.
Appendicitis Off The Grid After a disaster or at a remote homestead, the medic may not have ready access to modern medical technology. As such, many conditions that are commonly identified with ultrasounds or CAT scans may be more challenging to diagnose. One of these challenges is abdominal pain. There are various medical issues that cause it, with the classic example of an abdominal emergency being appendicitis. Over 300,000 Americans have their appendix removed every year. The Appendix The vermiform
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A prominent cosmetic surgeon and his daughter have lost a 2017 paper on treating men with excessive neck flab otherwise known as turkey neck because much of the work appears to have duplicated a book chapter he co-authored about the topic. The first author of the retracted article is Ronald L. Moy, a Continue reading Youth Guru loses turkey-neck paper that overlapped with book chapter
A new scientific study shows that bright electric light exposure of preschool children in the evening suppresses melatonin production almost completely, an important addition to the growing body of research in this area. Melatonin suppression is a marker of disruption of our circadian rhythms.
Ten kids, ages 3 to 5, were exposed to bright light (~1000 lux from a light box) for one hour before their habitual bedtime, about 8 p.m. Melatonin suppression (where the body stops producing this hormone) began within 10 minutes and continued for another hour after the bright light was turned off at 8 p.m., which was well into their usual sleep period. Melatonin is a hormone that is important for healthy circadian rhythms and good sleep.
This could undoubtedly reduce sleep quality, but may also cause other serious problems in the longer term.
The new study built upon a...
Im proud to be participating in the 3rd Chronic Lyme Disease Summit, a free, online summit beginning April 30th. RELATED STORY: Dr. Klinghardts Treatment of Lyme Disease >>>>>>>>>>>>Click here to sign up for FREE!<<<<<<<<<<<<<< After battling Lyme Disease for years, and finally having freedom from it, Im passionate about people finding that same thing for
Researchers say theyre well on the way to curing thousands of diseases by tinkering with human genes. But is that true? Or is their effort really part of a long-range agenda to keep experimenting in the dark, through grotesque trial and error, to alter humans and make them into a new species? (The Underground, Jon Rappoport)
With the onrush of new gene-editing techniques, the medical research establishment is beating an old drum: they will cure many human diseases by making genetic changes.
First of all, the new editing techniques have unknown consequences. A simple snip of a gene can bring on ripples in the patients overall genetic structure. This fact spells danger.
Second, and here is the old drum: there are a number of diseases caused by a problem with a single geneone gene, one disease. Therefore, a precise edit of the offending gene will cure the disease.
But is this one-gene one-disease hypothesis actually true?
If so, we should already have seen these cures. But we havent.
Im not talking about the occasional claim of a single cure in a single patient. Im talking about curing a specific disease across the...
By Dr. Mercola According to the largest review of the drug resistance problem to date, by 2050 the annual global death toll from antibiotic-resistant disease will reach 10 million. At present, an estimated 23,000 Americans die each year from drug-resistant infections, and the death toll will continue to rise until or unless the underlying causes are
(Editors Note: We love animals and we absolutely hate animal testing. Period. So it is with heavy hearts that we share this story with you.) Over the weekend, four baboons worked together to ESCAPE from the Southwest National Primate Reseach Center in San Antonio, Texas, one of seven federally supported National Primate Research Centers. However, they
The article below is a sponsored review for the BetterMe Fitness App
Were on a quest to find the fitness app an app with excellent reviews and excellent features that is actually good for you. Well, BetterMe reviews suggest that everything is okay in the customer satisfaction department, but will we be as excited about this app when we try it out?
Today, its so simple to download an app, use it for a bit and either delete and forget about it, or find out that you really like this tiny little helper and recommend it to your friends. The wellness and fitness app section is overflowing with options both on GooglePlay and AppStore, so the competition is kind of tough. What makes a certain fitness app stand out?
The BetterMe app is relatively new on the market, but it already tops the app charts and has thousands of mostly positive reviews. BetterMe Facebook community seems to be thriving as well, and they have their own site, BetterMe Guru, dedicated to health and wellness. So the BetterMe brand looks intriguing enough!
Youll need to enter your email, or register through Facebook, in order to set up an account with BetterMe.
The app makes use of some of your personal data you enter yourself, such as weight, age, height, target weight, your level of physical training (a newbie,...
Last April, the American Journal of Epidemiology and the American Journal of Public Health published a rare joint editorial statement. It concerned a pair of papers on the topic of mortality and obesity. Several complaints had prompted the journals to investigate. Their assessment: These papers contained inaccurate results. The statement was not a retractionit was Continue reading Dont like a paper, but dont want to retract it? Just issue an editorial statement
Many diabetics already know about the benefits of a low-glycemic diet and the need for regular exercise, but why haven't they heard about turmeric, one of the world's most extensively researched anti-diabetic plants?
From CNBC: A recent report by Goldman Sachs analysts questions whether curing patients, as opposed to providing them with chronic therapies, could pose a financial risk for biotech companies.
The potential to deliver one shot cures is one of the most attractive aspects of gene therapy, genetically-engineered cell therapy and gene editing. However, such treatments offer a very different outlook with regard to recurring revenue versus chronic therapies, analyst Salveen Richter wrote in the note to clients Tuesday. While this proposition carries tremendous value for patients and society, it could represent a challenge for genome medicine developers looking for sustained cash flow.'
From The Lily: In light of the recent New York Times article highlighting the effects antidepressant withdrawal, Ashley Abramson shares her personal story of deciding to come off antidepressants after taking them for almost 20 years.
A new randomized, double-blind, placebo-controlled trial has found bright light therapy to be a powerful intervention that could provide an alternative to medication for people with bipolar depression. Two-thirds of the people receiving the light therapy improved, compared with one-fifth of those who took only medication. The research was led by Dorothy Sit at Northwestern University and published in the American Journal of Psychiatry.
The study included people who had a diagnosis of either bipolar I or bipolar II disorder, but who were not currently meeting criteria for a manic or hypomanic episode. All participants were taking antimanic medication, also known as mood stabilizers (e.g. lithium).
Many (78.3%) of the participants were also taking antidepressant medications, and the researchers made sure that both the control group and the intervention group had similar numbers of people taking these drugs. All participants also met criteria for continuing depression, despite their current medication regimen.
One group of 23 participants was exposed to 7000-lux bright white light daily, between noon and 2:30 pm. They initially began at 15 minutes of light use, then gradually increased to the target dose of 60 minutes per day at week 4. The other group received a placebo light intervention that was described as 50-lux dim red light.
The difference in improvement is startling. 68.2% of people who received the bright light intervention no longer met criteria for depression (what researchers refer to as remission), compared with only 22.2% who received a placebo light intervention.
This difference was not due simply to an improvement in sleep quality, as sleep was observed to improve equally for both groups. There was no difference in side effects between the two groups, indicating that light therapy did not create any additional adverse events. No one experienced a hypomanic or manic episode.
The improvement in depressive symptoms appeared to occur between weeks 4-6 for most participants, which the researchers theorize is due to reaching the 60-minute per day dose of light at week 4 (see graph below).
Particularly surprising is that, despite most participan...
A MOVE to introduce a mineral to the water supply in a town where more than a third of five-year-olds have one or more decayed, filled or missing teeth has been revealed.
Some 63 years after being invited to take part in a five-year trial to test the benefits of adding fluoride to the water supply, Darlingtons council looks set to be urged to do so by one of its committees.
When it rejected the scheme in 1955 members of Darlington Town Council said they feared they were making a mistake, but residents did not all want fluoride in their water.
Seven years later, the Ministry of Health published the studys results, which found there had been a substantial improvement in the teeth of young children in the areas which agreed to the study and no evidence of harm from fluoridation had been discovered.
At a meeting of the councils children and young people scrutiny committee members heard a proposal to introduce fluoride to the towns water supply was being drawn up following a study which exposed stark differences between childrens oral health between areas where the mineral is or is not in drinking water.
Members were told childrens oral health was also closely linked to deprivation, but there were huge differences between the economically similar areas of Darlington and Hartlepool, where the latter has fluoride naturally in the water.
Councillor Anne-Marie Curry said fluoridated water was the least intrusive way of helping people look after their teeth.
She told the meeting: There is still a feeling that there should be a choice. If they dont want the fluoridated water we should suggest they filter it or drink bottled water.
Last year, Dr John Furness, a consultant paediatrician based at Darlington Memorial Hospital, said despite weekly extraction clinics for youngsters in the region, councils were refusing to pay for the fluoride to water because of the cost involved.
Durham County Council has heard while 61 per cent of children were suffering decay in the Woodhouse Close area of Bishop Auckland, just six per cent were in the Chester-le-Street South division, where fluoride has been added to the water supply since the 1960s, at a cost of around 50,000 a year.
However, campaigners say there is no conclusive evidence about the benefits of adding the mineral to the water supply.
After being told about the possible move in Darlington, Professor of Health Policy at Kent University Stephen Peckham said schemes which had seen NHS staff support families were far more effective than fluoridation....
By Dr. Mercola
According to the largest review of the drug resistance problem to date, by 2050 the annual global death toll from antibiotic-resistant disease will reach 10 million.1 At present, an estimated 23,000 Americans die each year from drug-resistant infections, and the death toll will continue to rise until or unless the underlying causes are properly addressed. One significant driver of antibiotic-resistant bacteria is the routine use of antibiotics in agriculture.2
As noted by the Institute for Agriculture and Trade Policy in 2016,3 "The rapid spread of new disease strains ... is one very visible reason why the expansion of factory-style animal production is viewed as unsustainable." In the video above, the late Steve Wing, former associate professor and epidemiologist at the University of North Carolina at Chapel Hill, discusses the human health impacts of factory farms.
Another major contributor to and source of drug-resistant infections is hospitals. According to statistics by the Centers for Disease Control and Prevention (CDC), 1 in 25 patients now contracts a hospital-acquired infection4 and many of them are resistant to drug treatment. Most disturbing of all, some infections are now exhibiting "panresistance," meaning they're resistant to every antibiotic in existence.
One particularly nasty panresistant bacterium that is gaining ground is carbapenem-resistant enterobacteriaceae (CRE), which produces an enzyme that breaks down antibiotics. Hospitals are the most common source of this infection, which is lethal in about 9 percent of all cases. When the CRE infection affects the blood, the death rate jumps to 50 percent.5
Tests conducted last year on nearly 5,780 antibiotic-resistant bacterial samples collected from hospitals and nursing homes revealed 1 in 4 samples contained genes known to confer drug resistance, and 221 contained a particularly rare drug-resistance gene that confers a very high level of resistance.6,...
By Dr. Mercola
Carrageenan, a food additive extracted from red seaweed, is commonly added as a thickening agent to processed foods, particularly dairy products, certain deli meats and other prepared foods. Since it comes from seaweed, many people assume carrageenan is natural perhaps even healthy and along with conventional foods this additive is often found in natural and organic products.
The problem is that carrageenan is not nutritious, nor is it natural or certified organic. Its a processed additive extracted from seaweed using alkali, and research suggests its highly inflammatory, triggering an immune reaction that may cause inflammation in your gastrointestinal system and related problems.1 As such, organic watchdog groups such as The Cornucopia Institute have called for it to be removed from the U.S. list of approved organic ingredients.
In December 2016, the National Organic Standards Board (NOSB), the U.S. Department of Agricultures (USDA) expert advisory board, voted to do just that. After hearing evidence on the potential health risks and the availability of alternative ingredients, NOSB voted to remove carrageenan from the organic ingredients list. Unfortunately, the vote is technically only a recommendation, and while the USDA has historically almost always sided with their expert panel, in April 2018 they did just the opposite.
AMS [USDA's Agricultural Marketing Service] has reviewed NOSB's sunset review document and decided to renew all 17 substances, including carrageenan. AMS found sufficient evidence in public comments to the NOSB that carrageenan continues to be necessary for handling agricultural products because of the unavailability of wholly natural substitutes
Carrageenan has specific uses in an array of agricultural products, and public comments reported that potential substitutes do not adequately replicate the functions of carrageenan across the broad scope of use.
Yet, as noted by Mark A. Kastel, senior farm policy analyst at the Cornucopia Institute, the decision flies in the face of the very reason NOSB was created. This is the latest instance of the administration...
By Dr. Mercola
Though he still sees himself as just a little boy who loves frogs, Tyrone Hayes, Ph.D., an integrative biologist at the University of California, Berkeley, has spent a decade feuding with chemical giant Syngenta, manufacturer of the pesticide atrazine. Novartis, which eventually became Syngenta, asked Hayes to use his extensive knowledge of frog hormones to determine if atrazine was interfering with frogs in the environment.
It certainly wasnt what they wanted to hear, but he found atrazine may be chemically castrating male frogs, essentially turning them into female frogs. When Hayes reported some of his troubling findings to the U.S. Environmental Protection Agency (EPA), including male frogs with eggs bursting out of their testicles I thought they would be interested, after all it was the No. 1 selling chemical in the world at the time, he said they stated it wasnt an adverse effect that would prompt them to reevaluate the chemical.
Hayes resigned his contractor position with Syngenta after the company refused to allow him to publish the results of studies they had funded. After resigning, he obtained independent funding to repeat the research, which was subsequently published and found that atrazine causes hermaphroditism in frogs.
Syngenta attempted to discredit Hayes after the damaging research was released. However, as Hayes argues in the TED talk above, he follows Albert Einsteins notion that those who have the privilege to know, have the duty to act.
Hayes research hypothesized that atrazine turned on an enzyme (aromatase) that caused testosterone to be converted into estrogen. If youre a male, this means that you wont make sperm, but you will make estrogen, even though you shouldnt.
According to Hayes and colleagues in research published in Nature in 2002, exposure to water-borne atrazine contamination led to gonadal abnormalities such as retarded development and hermaphroditism in 10 percent to 92 percent of male wild leopard frogs. Hayes published another study in 2010 in Proceedings of the National Academy of Sciences (PNAS), which similarly found:1
Atrazine-exposed males suffered from depressed testosterone, decreased breeding gland size, demasculinized/feminized laryngeal development, suppressed mating behavior, reduced sper...
This video details the Left's flagrant attacks on Christians. Whether it be attacks against Chick-Fil-A, Mike Pompeo, or Christian bakers, those of the left have proven that they are intolerant of Christianity.
Currently our total stands at $49,364 from 331 donors. Thanks to everyone who has donated so far and for everything else you and they are doing to end the reckless practice of water fluoridation.
The TSCA lawsuit* against the EPA has sparked three creative ways to increase money for the legal fund.
The first flare of creativity comes from David Green in Ontario, who sent us this photo of his effort. His local group is Fluoride Free Peterborough. David is a partner, with his brother Ian, in Rocky Ridge Drinking Water Limited. David says, No $50 bills in the bottle yet, but the coins are accumulating.
The second is a challenge is from one of FANs long-time benefactors
The third is also a challenge from yet another long-time (since the 1970s) fluoridation fighter, who firmly believes that this lawsuit is our best chance ever of ending fluoridation worldwide.
Our goal is to raise $75,000 by May 31. If we make that deadline then we have another generous benefactor and a non-profit organization that have each pledged to donate $75,000 to yield a healthy legal fighting fund of $225,000. Ideally, we would like the burden to be shared by a 1000 people.
How to donate
Margaret van Zyl is still waiting for her money.
The Mosgiel pensioner turned up at the Dunedin City Councils infrastructure and networks committee meeting yesterday to voice her displeasure during the public forum.
She was upset by the councils continuing practice of adding fluoride to drinking water, in line with Ministry of Health guidelines.
And she was also upset at the councils decision to switch the source of Mosgiels water from an untreated bore to the citys fluoridated supply in December, following public health advice.
But, most of all, she was miffed by the councils refusal to pay for the $240 water filter she bought and had installed after Mosgiels water supply changed.
Mrs van Zyl told yesterdays meeting she had sent the bill the council, but received a response from council chief executive Sue Bidrose declining to cover the cost.
Not good enough, Mrs van Zyl told councillors.
I expect to be paid. Your cheque, I hope, will be in the post.
*Original article online at https://www.odt.co.nz/news/dunedin/dcc/council-gets-ticking-over-bill
PMID: Br J Nutr. 2018 Apr ;119(8):847-858. PMID: 29644950 Abstract Title: Vitamin C intake in relation to bone mineral density and risk of hip fracture and osteoporosis: a systematic review and meta-analysis of observational studies. Abstract: We aimed to systematically review available data on the association between vitamin C intake and bone mineral density (BMD), as well as risk of fractures and osteoporosis, and to summarise this information through a meta-analysis. Previous studies on vitamin C intake in relation to BMD and risk of fracture and osteoporosis were selected through searching PubMed, Scopus, ISI Web of Science and Google Scholar databases before February 2017, using MeSH and text words. To pool data, either a fixed-effects model or a random-effects model was used, and for assessing heterogeneity, Cochran's Q and I 2 tests were used. Subgroup analysis was applied to define possible sources of heterogeneity. Greater dietary vitamin C intake was positively associated with BMD at femoral neck (pooled r 018; 006, 030) and lumbar spine (pooled r 014; 95 % CI 006, 022); however, significant between-study heterogeneity was found at femoral neck: I 2=876 %, P heterogeneity
PMID: Spine (Phila Pa 1976). 2018 Apr 11. Epub 2018 Apr 11. PMID: 29649092 Abstract Title: Ascorbic Acid Attenuates Multifidus Muscles Injury and Atrophy after Posterior Lumbar Spine Surgery by Suppressing Inflammation and Oxidative Stress in a Rat Model. Abstract: STUDY DESIGN: A rat model of multifidus muscles injury and atrophy after posterior lumbar spine surgery.OBJECTIVE: We determined the effect of ascorbic acid (AA) on the postoperative multifidus muscles in rat model.SUMMARY OF BACKGROUND DATA: Previous studies show oxidative stress and inflammation are two main molecular mechanisms in multifidus muscle injury and atrophy after posterior lumbar surgery. AA may have a protective effect in postoperative multifidus muscles.METHODS: Rats were divided into sham surgery, control surgery, and surgery plus AA groups. Multifidus muscles of the control and AA groups were excised from the osseous structures. The muscles were retracted continuously for 2h. In the sham and AA groups, AA was administered via oral gavage daily in the first week. In each group, the oxidative stress was evaluated by measuring malondialdehyde (MDA) and Total superoxide dismutase (T-SOD). The inflammation, fat degeneration or fibrosis of multifidus muscle were evaluated by Q-PCR, histology or immunohistochemical analysis.RESULTS: T-SOD activity was significantly lower in the control group than that in the AA group in the first week. MDA levels were significantly higher in the AA group. Interleukin-6 and tumor necrosis factor- in multifidus muscles also showed significant differences when treated with AA. The inflammation score on histology was significantly lower in the AA group postoperatively in the first week. In the long run, marker genes for fibrosis and fat degeneration, and fibrosis and fat degeneration scores,were significantly lower in the AA than the control group on days 14 and 28 postoperatively.CONCLUSIONS: In conclusion, AA attenuated the oxidative stress and inflammation response in the postoperative multifidus muscles, and remarkable differences were observed from the histological assessment and related marker genes expression. Our results provided important insight into the anti-inflammatory and anti-oxidative effects of AA in the postoperative multifidus muscles.LEVEL OF EVIDENCE: N/A.
PMID: Ren Fail. 2018 Nov ;40(1):298-305. PMID: 29619876 Abstract Title: Inhibitory effects of taraxasterol and aqueous extract of Taraxacum officinale on calcium oxalate crystallization: in vitro study. Abstract: OBJECTIVE: We investigated and compared the effects of taraxasterol, aqueous extract of T. officinale (AET) aerial part, and potassium citrate (PC) on calcium oxalate (CaOx) crystallization in vitro.MATERIALS AND METHODS: CaOx crystallization was induced by adding sodium oxalate to synthetic urine. Taraxasterol (2.5, 5, 7.5 and 12.5g/mL), extract (1, 2, 4 and 8mg/mL), and PC (100, 150, 200 and 350mg/mL) were subjected to anti-crystallization activities. The absorbance and %inhibition of nucleation of CaOx crystals were evaluated by spectrophotometer at 2, 4, 6, 8, 10, 20, 30, 40, 50 and 60min and the number and morphology of crystals were studied by light microscopy after 60min.RESULTS: Presence of taraxasterol, extract and PC decreased absorbance in experimental samples compared to control, significantly. The nucleation of crystals is inhibited by taraxasterol, extract, and PC (26-64, 55-63 and 60-70%, respectively). The number of CaOx crystals were decreased in presence of taraxasterol (p
PMID: PLoS One. 2018 ;13(3):e0194843. Epub 2018 Mar 22. PMID: 29566095 Abstract Title: Metabolic recovery from heavy exertion following banana compared to sugar beverage or water only ingestion: A randomized, crossover trial. Abstract: OBJECTIVES AND METHODS: Using a randomized, crossover, counterbalanced approach, cyclists (N = 20, overnight fasted state) engaged in the four 75-km time trials (2-week washout) while ingesting two types of bananas with similar carbohydrate (CHO) but different phenolic content (Cavendish, CAV; mini-yellow, MIY, 63% higher polyphenols), a 6% sugar beverage (SUG), and water only (WAT). CHO intake was set at 0.2 g/kg every 15 minutes. Blood samples were collected pre-exercise and 0 h-, 0.75 h-,1.5 h-, 3 h-, 4.5 h-, 21 h-, 45 h-post-exercise.RESULTS: Each of the CHO trials (CAV, MIY, SUG) compared to water was associated with higher post-exercise plasma glucose and fructose, and lower leukocyte counts, plasma 9+13 HODES, and IL-6, IL-10, and IL-1ra. OPLS-DA analysis showed that metabolic perturbation (N = 1,605 metabolites) for WAT (86.84.0 arbitrary units) was significantly greater and sustained than for CAV (70.43.9, P = 0.006), MIY (68.34.0, P = 0.002), and SUG (68.14.2, P = 0.002). VIP ranking (
PMID: J Food Sci Technol. 2018 Feb ;55(2):658-666. Epub 2017 Nov 27. PMID: 29391630 Abstract Title: Extraction and characterization of proteins from banana () flower and evaluation of antimicrobial activities. Abstract: Ultrasonic assisted alkaline extraction of protein from banana flower was optimized using response surface methodology. The extracted proteins were characterized by Fourier transform infrared spectroscopy and molecular weight distribution was determined by gel electrophoresis. The maximum protein yield of 252.25 mg/g was obtained under optimized extraction conditions: temperature 50 C, 30 min extraction time and 1 M NaOH concentration. The alkaline extraction produced a significantly high protein yield compared to enzymatic extraction of banana flower. Chemical finger printing of proteins showed thepresence of tyrosine, tryptophan and amide bonds in extracted protein. Alkaline and pepsin assisted extracted banana flower proteins showed characteristic bands at 40 and 10 kDA, respectively. The extracted proteins showed antibacterial effects against both gram positive and gram negative bacteria.The high protein content and antimicrobial activity indicate the potential applications of banana flower in the food and feed industry.
PMID: Pharmacognosy Res. 2018 Jan-Mar;10(1):49-54. PMID: 29568187 Abstract Title: Anticonvulsant and Antioxidant Effects ofStem Extract on Acute and Chronic Experimental Models of Epilepsy. Abstract: Background: (banana) plant extract has been shown to possess antioxidant activity in previous studies. Neuronal injury resulting from oxidative stress is an important factor involved in pathogenesis of epilepsy.Objective: The present study aimed to evaluate the anticonvulsant activity ofstem extract (MSSE) in acute and chronic experimental models in mice and its effects on various markers of oxidative stress in the brain of pentylenetetrazole (PTZ)-kindled animals.Material and Methods: Maximal electroshock seizures (MES) and PTZ-induced convulsion models were used for acute studies. For the chronic study, the effect of MSSE on the development of kindling was studied. For the evaluation of the effects of MSSE on oxidative stress in brain, malondialdehyde (MDA) and reduced glutathione (GSH) levels were estimated in the brains of the kindled animals.Results: MSSE significantly increased the latency to onset of myoclonic jerks and the duration of clonic convulsions following PTZ administration. The MSSE pretreated group showed significantly reduced mean seizure score on PTZ-induced kindling. There was a significant increase in the brain MDA levels and decrease in GSH levels in response to PTZ-induced kindling. On MSSE pretreatment, there was a significant decrease in the MDA levels in the brains, though the increase in the GSH levels was not significant.Conclusion: The results from this study suggest the presence of significant anticonvulsant activity in MSSE, in both acute and chronic PTZ-induced seizure models, which could be due to its antioxidant activity, as is reflected by the change in oxidative stress markers in brain.SUMMARY: Evaluation of the anticonvulsant activity ofand its effects on various markers of oxidative stress in the brain has not been done previously to the best of our knowledgestem extract (MSSE) significantly increased the latency to onset of myoclonic jerks and the duration of clonic convulsions in the experimental modelsThe MSSE pretreated group showed significantly reduced mean seizure score on pentylenetetrazole (PTZ)-induced kindlingThere was significant increase in the brain malondialdehyde (MDA) levels and decrease in glutathione (GSH) levels in response to PTZ-induced kindlingOn MSSE pretreatment, there was a significant decrease in the MDA levels in the brain, though the increase in the GSH levels was not significant.MSSE:stem extract, PTZ: Pentylenetetrazole, MES: Maximal electroshock seizures, MDA: Malondialdehyde, GSH: Glutathione, SOD: Superoxide dismutase, THLE: Tonic hindlimb extension.
PMID: Food Sci Nutr. 2018 Jan ;6(1):137-145. Epub 2017 Nov 20. PMID: 29387371 Abstract Title: Antihyperglycemic and antidyslipidemic activity of-based diet in alloxan-induced diabetic rats. Abstract: This study was aimed at investigating the antihyperglycemic and antidyslipidemic activity of-based diets in alloxan-induced diabetic mellitus rats. Diabetes was induced by a single intraperitoneal injection of alloxan (150 mg/kg b.w) in 48 randomly selected rats. The rats were randomly grouped into four as follows: normal rats fed-based diet, diabetic control rats fed-based diet, diabetic rats fed-based diet and administered metformin (14.2 mg/kg body weight) orally per day, and diabetic rats fed-based diet. Body weight and fasting blood glucose level were monitored, on 28th days the rats were sacrificed, liver was excised. Thereafter, the hyperglycemic and dyslipidemic statii of the induced diabetic animals were determined. The-based diet significantly (
Dane Wigington GeoengineeringWatch.org Planet Earth is being forced into an abrupt climate shift, the ongoing global climate engineering operations are further fueling the overall process. The combination satellite / radar image below reveals an extremely anomalous and alarming scenario: a massive, bizarre, and completely unnatural, straight, south to north flow of tropical moisture from the record warm waters
PMID: Indian J Pharmacol. 2017 Jan-Feb;49(1):71-76. PMID: 28458426 Abstract Title: Antidiabetic, antihyperlipidemic, and antioxidant activities ofColla. in Type 1 diabetic rats. Abstract: OBJECTIVES: To evaluate the antidiabetic, antihyperlipidemic, and antioxidant activities of the ethanolic extracts of the flowers and inflorescence stalk ofColla. in streptozotocin (STZ)-induced Type 1 diabetic rats.MATERIALS AND METHODS: Diabetes was induced in male Wistar albino rats (150-200 g) by single intraperitoneal injection of STZ (60 mg/kg b.w. i.p.). Albino rats (= 25) were divided into five groups, of which five animals each. Group A (normal control) and Group B (diabetic control) received normal saline (10 ml/kg/day p.o.), whereas Group C and Group D received 250 mg/kg/day p.o. of flower and inflorescence stalk ethanolic extracts, respectively, for 2 weeks. Group E (diabetic standard) received 6 U/kg/day s.c of Neutral Protamine Hagedorn insulin. Fasting blood sugar, serum insulin, catalase (CAT), malondialdehyde (MDA), and serum lipid profile were estimated at specific intervals of time. Effect of the extracts on intestinal glucose absorption was also evaluated to know the probable mechanism of action.RESULTS: Diabetic control exhibited significant increase in blood glucose, serum cholesterol, triglycerides, low-density lipoprotein, serum MDA levels and decreased serum CAT, and high-density lipoprotein levels which were significantly reverted by flower and inflorescence stalk ethanolic extracts after 2 weeks. Serum insulin levels were in increased (
PMID: Carbohydr Polym. 2017 May 15 ;164:31-41. Epub 2017 Jan 22. PMID: 28325331 Abstract Title: Two banana cultivars differ in composition of potentially immunomodulatory mannan and arabinogalactan. Abstract: Banana (Musa acuminata and M. acuminata x M. balbisiana) fruit cell walls are rich in mannans, homogalacturonans and xylogalacturonan, rhamnogalacturonan-I, and arabinogalactans, certain forms of which is considered to have immunomodulatory activity. The cultivars Nanico and Thap Maeo represent two widely variants with respect to compositional differences in the forms of these polysaccharides. Nanico has low amounts of mannan in the water-insoluble and water-soluble fraction. Both cultivars have high amounts of water-soluble arabinogalactan. These commelinoidmonocots lack the (13),(14)--d-glucans of grasses, but Thap Maeo has higher amounts of non-starch glucans associated with wild species than does Nanico. High amount of callose was found in both cultivars. As immunomodulatory activity is associated with the fine structure and interaction ofthese polysaccharides, breeding programs to introgress disease resistance from wild species must account for these special structural features in retaining fruit quality and beneficial properties.
Pain or headaches at the base of your skull are often the result of tight muscles in the back of your neck due to tension, stress, or fatigue. Pain in back of head at the base of skull can also be caused by a herniated cervical disc or injury to your upper neck. Sometimes, the headaches that start at the base of the skull can be tension headaches, cluster headaches, or cervicogenic headaches.
Pain in the back of your head at the base of your skull can cause your head to hurt with dull, nagging persistent pains. If you have a trapped nerve in your cervical spine, you may experience sharp jabbing pains that radiate to your temples or behind your eye. Headaches at the back of your neck can also cause pain that travels down your spine. Neck pain at the base of your skull may also result in shoulder pain, back pain, or tingling in your arms.
In most cases, the neck pain and headaches at the back of your head go away when you get proper rest or apply home remedies. For example, applying a heat or cold pack, getting a neck or shoulder massage, or using essential oils can help to relieve a stiff neck that causes headaches.
In this article, you will find out what it means if you have a headache in the back of your head and neck. You will also find helpful ways to alleviate the pressure and tension at the base of your skull. In some cases, if the pain doesnt go away, you should see a doctor.
The upper part of your spine that joins to the base of your skull is called the cervical spine. Dr. Robert E. Windsor, who specializes in physical medicine and rehabilitation, says that the cervical spine consists of 7 vertebrae. These are referred to as C1 to C7 vertebrae. The first vertebra (C1) is called the atlas and upon which the base of the skull rests.1
The cervical spinal vertebrae are also responsible for most of the movement of your neck and head. Dr. Windsor says that the cervical spine is the most mobile part of your spine.
Intervertebral discs are located between each vertebra and these act as shock absorbers. These are round, jelly-like discs that prevent inflammation and pain in the back of your neck.
The Encyclopaedia Britannica says that you also have nerves and muscles in the back of your neck. The muscles join the top of your neck to the base of your skull. The trapezius muscles also attach your neck to your scapula (shoulder blade). There is also a system of nerves in the back of your neck, some of which form part of the spinal cord.2
What Southerner does not love barbecue? This classic recipe for pulled pork is one of my redone favorites using healthier ingredients. It is oven baked for convenience (who has a smoker in the backyard?), but the results turn out just as tender.
This dish is just perfect for a crowd or a chilly/rainy weekend at home.
The post Southern Pulled Pork Recipe (oven baked, sugar free) appeared first on The Healthy Home Economist.
Do you ever feel like medical tests and screenings that are supposedly expected of you are just too much? Annual physical exams are one thing, but then you have cancer screenings, pap smears, eye exams, colonoscopies, breast exams All those appointments are done in hopes of keeping you healthy and extending your life. However, New York... View Article
The post Why Barbara Ehrenreich Is Giving Up On Preventative Care appeared first on Healthy Holistic Living.
by Paul Fassa
Health Impact News
Sweat lodges, dry saunas, and wet saunas (steam) have been used for healing and detoxing for several centuries. While exposing ones body to heat over an extended period of time, the activity of fever takes place, stimulating the immune system to release white blood cells to kill pathogenic microbes or destroy cancer tumor cells. (Source)
Exposing heat to the body over an extended period of time produces profuse sweating that can transport toxins out of the body. Over time, the practice of sauna bathing can even eliminate toxins and drugs trapped in lipid tissue (fat) for years.
Both functions of body heat is why more and more health practitioners are realizing that wrapping up and sweating out a bout of fever up to but not beyond 104 degrees Fahrenheit is more beneficial than suppressing it with pharmaceuticals. (Source)
Many of us cant cope with extended dry or wet sauna visits and some of us cant even access them. But there is a solution for easily obtaining the same results without the agony of enduring intense ambient heat, using infrared technology.
Infrared energy frequency applications offer deeper heat penetration into body tissue with less heat, reducing ambient heat from the 160 degrees F of conventional saunas to 110 degrees. Infrared radiation is able to penetrate human tissue three inches, less or more, without burning or discomfort.
Generally, around 15 to 30 minutes inside an infrared radiation sauna is sufficient to achieve the maximum expected results of conventional dry or wet saunas that require enduring higher temperatures for considerably longer times.
Mayo Clinics Dr. Brent...
PMID: Carbohydr Polym. 2015 Jun 25 ;124:157-63. Epub 2015 Feb 20. PMID: 25839806 Abstract Title: A polysaccharide from Trametes robiniophila inhibits human osteosarcoma xenograft tumor growth in vivo. Abstract: In the present study, we isolated and purified one polysaccharide (TRP) from Trametes robiniophila, which had a backbone of 1,3,6- and 1,4-linked glucpyranosyl moieties, with 1-linked arabinofuranosyl and galactopyranosyl terminal at the O-3 position of 1,3,6-linked glucpyranosyl residues. TRP was further evaluated for its antitumor activity against xenografted U-2 OS osteosarcoma in BALB/c nude mice together with the possible mechanism of action. We found that oral administration of TRP significantly suppressed U-2 OS tumor growth in mice via the induction of apoptosis, as evidenced by the increased number of TUNEL-positive cells in tumor tissues. Moreover, TRP administration increased the levels of the proapoptotic Bax protein and decreased the level of the antiapoptotic Bcl-2 protein, thus resulting in a rise of Bax/Bcl-2 ratio. Furthermore, the protein expression of caspase-9, caspase-3 and cleaved PARP became evident in tumor tissues from mice following TRP treatment, but caspase-8 keep unchanged. Besides, overexpression of metadherin (MTDH) was attenuated in tumor tissues of TRP-fed mice. Taken together, these findings suggest that the TRP-induced apoptosis of tumor tissues is through a mitochondria-mediated intrinsic apoptotic pathway.
Smartphones are a permanent fixture in most peoples lives, a constant source of notification and quasi-connection. The downside of that convenience is that many of us are also addicted to the constant pings, chimes, vibrations and other alerts from our devices, unable to ignore new emails, texts and images. We are addicted to logging in.
In a new study published in NeuroRegulation, San Francisco State University Professor of Health Education Erik Peper and Associate Professor of Health Education Richard Harvey argue that overuse of smart phones is just like any other type of substance abuse.
On top of that, addiction to social media technology may actually have a negative effect on social connection. In a survey of 135 San Francisco State students, Peper and Harvey found that students who...
PMID: Zhongguo Zhong Yao Za Zhi. 2015 Dec ;40(24):4805-10. PMID: 27245026 Abstract Title: [Research progress on anti-tumor effect of Huaier]. Abstract: Huaier (Trametes robiniophila) has been widely used as an adjuvant drug for cancer treatment in China. The anti-cancer effect of Huaier extract has been confirmed in liver cancer, lung cancer, breast cancer, ovarian cancer, gastric cancer, and so on. The main mechanisms by which Huaier exerts an anti-neoplastic effect include inhibition of the growth and proliferation of cancer cells, induction of apoptosis of cancer cells, suppression of angiogenesis, inhibition of the invasion and migration of cancer cells, regulation of oncogenes and tumor suppressor genes expression, improving immunity, and reversal of drug resistance in cancer cells. In order to provide references for further study and clinical application on anti-tumor effect of Huaier, the latest research progress on anti-tumor effect of Huaier in recent years is summarized in this paper.
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