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Saturday, 17 February

21:37

Fluoridation debate continues in Clark County "IndyWatch Feed Health"

City officials in central Ohio are considering fluoridating the municipal water supply to combat poor dental health.

The Springfield News-Sun reports Clark County Health Commissioner Charles Patterson urges Springfield city commissioners to allow a referendum vote in November on fluoridating the water supply.

A state Department of Health study says about two-thirds of children in Clark County has experienced tooth decay. Patterson says the chief complaint among patients at Springfield Regional Medical Centers emergency room in 2015 was oral health-related pain.

Patterson says fluoridation is safe and the most effective way to prevent oral health decay.

Opponents of the measure say fluoridation is harmful and the county should instead focus on expanding dental coverage for Medicaid patients.

Ballot initiatives for the November election must be filed by Aug. 8.

17:20

Age of Reactive Attachment Disorder? "IndyWatch Feed Health"

Our community is none too pleased to see reports that the Florida shooter "may have had autism." Our children, from tots to teens to twenties and beyond, are not innately cruel. They may be terribly violent at times most often...

17:13

New Utah Law Would Expand Raw Milk Sales From Farms and Markets to Mobile Trucks "IndyWatch Feed Health"

By Michael Maharrey

Yesterday, a Utah Senate committee passed a bill would expand raw milk sales in the state. Passage into law would take an important step toward rejecting a federal prohibition scheme in effect.

Sen. David Hinkins (R-27) and Rep. Marc Roberts (R-67) introduced Senate Bill 108 (SB108) January 23. The legislation would expand existing laws relating to raw milk sales from producer to consumer in the state. Under the proposed law, a milk producer would be able to sell up to 120 gallons of raw milk per month to consumers without meeting stricter requirements under the current law and permitting program, providing certain conditions are met. Under the proposed law, the raw milk could only be sold directly to the consumer on the premises where the milk is produced for household use, not resale. SB108 also includes handling, sanitation and record-keeping requirements for unlicensed producers of raw milk. The milk bottle would have to include the warning This raw milk has not been licensed or inspected by the state of Utah. Raw milk, no matter how carefully produced, may be unsafe.

The proposed law would also allow licensed producers to sell raw milk from a mobile refrigerated truck where the raw milk is maintained at 41 degrees Fahrenheit or a lower temperature.

The Senate Natural Resources, Agriculture, and Environment Committee...

16:49

Prevention Against Gallstones Eat More Foods With Vitamin E "IndyWatch Feed Health"

By Karen Foster

More than 90 percent of the population does not meet their recommended daily allowance of vitamin E. Researchers in Germany led by the University of Kiel found that subjects with gallstone disease had lower blood levels of vitamin E than those who did not have gallstones.

Gallstones are hardened deposits of digestive fluid that can form in your gallbladder. Gallstones range in size from as small as a grain of sand to as large as a golf ball. Some people develop just one gallstone, while others develop many gallstones at the same time. Most gallstones provoke no symptoms at all. On average, symptoms take about eight years to develop.

Vitamin E does everything from ease exercise soreness, treating menstrual pain, preventing cancer and playing a central role in neurological and cardiovascular function. Adequate levels of vitamin E, an essential micronutrient, are especially critical for the very young.

The scientists also found that those with gallstone disease also had a lower alpha-tocopherol/cholesterol ratio than healthy individuals. A similar association was found with gamma-tocopherol levels. However, with the gamma form, the results were not statistically significant.

We observed an inverse association between circulating vitamin E (alpha- and gamma-tocopherol [non-significant]) levels and the prevalence of gallstone diseas...

16:41

The Really Weird World Of Smart Meters "IndyWatch Feed Health"

By Catherine J. Frompovich

Probably when those who either question and/or oppose AMI Smart Meters thought the situation couldnt get any more weird than it is regarding misinformation and lack of transparency about the so-called consensus science that dominates technology, medicine, pharmacology and vaccines, the makers of one high-profile Smart Meter, Landis+Gyr seemingly has outdone itself.

The website Blacklisted News featured an article titled Smart Meter Company Landis+Gyr Now Using Copyright To Try To Hide Public Records. What happened to transparency, especially in high tech science? Is there information they dont want the public to know?

As some background information, back in 2016 L+G took some legal action, which was chronicled in the Tech Dirt article Smart Grid Company Demands MuckRock Turn Over Info On Anyone Who Might Have Seen Public Records Docs Involving It.

Hmmm!

16:14

NJ Hospital Says More Flu Shots Key To Immune System "IndyWatch Feed Health"

Its the flu season that simply wont go away. Many people are questioning how hospital workers, such as nurses, are surviving. One New Jersey hospital claims that the more flu shots you get, the better...

The post NJ Hospital Says More Flu Shots Key To Immune System appeared first on Vaxxter.

14:26

The REAL STORY About the Skeptic Attack on Del BigTree "IndyWatch Feed Health"

Are We Sick of The Threats, Lies and Misdirection? Opinion by Deplorable Consumer Advocate Tim Bolen The photo at the top of this article is indeed worth a thousand words, for it explains a lot about WHO, and WHAT, attacked Vaxxed producer Del BigTree. Lets explore that WHO, and WHAT.  Yes, the attack was easily repulsed Continue reading The REAL STORY About the Skeptic Attack on Del BigTree

The post The REAL STORY About the Skeptic Attack on Del BigTree appeared first on BolenReport.

12:03

Statement of APA President in Response to Florida Shooting "IndyWatch Feed Health"

APA President Jessica Henderson Daniel has released a statement on the recent school shooting in Florida emphasizing the lack of correlation between gun violence and mental health.

While law enforcement is still piecing together the shooters motives, some public figures and news reports are focusing on his mental health. It is important to remember that only a very small percentage of violent acts are committed by people who are diagnosed with, or in treatment for, mental illness. Framing the conversation about gun violence in the context of mental illness does a disservice to the victims of violence and unfairly stigmatizes the many others with mental illness. More important, it does not direct us to appropriate solutions to this public health crisis.

Article 

12:02

When You Start Paying Attention to Everyday Sensory Experience "IndyWatch Feed Health"

From Aeon: A new video aims to capture the small, abstract moments that make up our inner lives, that would otherwise go ignored or quickly disappear.

Video 

12:01

Michael Fontaine: What the Ancient World Can Teach Us About Emotional Distress "IndyWatch Feed Health"

This week on MIA Radio, we interview Professor Michael Fontaine. Michael is Professor of Classics and Associate Vice Provost of Undergraduate Education at Cornell University in New York. He regularly consults on Latin for museums, institutions, dealers, and collectors, having exposed forgery in Renaissance and Dutch Golden Age paintings. In 2016 he received the Thomas S. Szasz Award for Outstanding Contributions to the Cause of Civil Liberties.

In the episode we discuss:

  • How Michael came to be a Professor of Classics and Literature.
  • How studying the ancient world helps us to understand what the first scientists thought about mental or emotional distress.
  • The first use of the phrase psychiatric ward which can be found at the Library of Alexandria in Northern Egypt.
  • That the phrase that ultimately became the word Psychiatry in ancient times actually meant a Healing Place for the Soul and is inscribed above library entrances even today ( or Psyches iatreion).
  • The links between the Rosenhan experiment and a comedic play written 2,200 years ago by the ancient Roman playwright T. Maccius Plautus.
  • That, in the ancient world, there was no long-term incarceration in prisons or asylums.
  • The relationship between the Hippocratic/medical model (the humoral theory) and the Epicurean model of mental or emotional distress.
  • That, in the ancient world, depression didnt exist, and that the solutions for unhappiness were based in the community or in Philosophy.
  • That about 1700 years ago, the Roman Empire entered a state of decline and it...

12:01

Congress Did Something Extraordinary for Vulnerable Children "IndyWatch Feed Health"

From The Intercept: A new piece of legislation attempting to revolutionize the foster care system recently passed despite significant opposition. The Family First Prevention Services Act allows states to use federal funds for preventative mental health and substance use programs to keep at-risk children in their homes instead of the foster system.

The Family First Prevention Services Act will usher in the most significant improvements to the child welfare system in decades and provide real help to families to fight the opioid epidemic, Wyden said in a statement. We owe our most vulnerable children the best chance to stay with their families when its safe to keep them at home and the highest standards of care to protect children who are already in foster care.

Children are taken from their families in cases of abuse but more often than not, Sandy Santana, executive director for Childrens Rights, said, kids enter the foster care system because of neglect that stems primarily from issues of poverty or substance abuse.

With the opioid epidemic, more and more kids are coming into the system because their parents are dependent on opioids, Santana said. For the government now to redirect funding for substance abuse and preventative services to keep those kids that are at risk of entering foster care with their families is a big, big deal.'

Article

12:00

Pharmagunndon in America "IndyWatch Feed Health"

Note: Teresa Wrote this post in 2012, after the shooting by Adam Lanza in Newtown, CT. Seems this post should just be "on deck" at any given moment in the USA. By Teresa Conrick Here at Age of Autism, we...

11:18

Dietary phytoestrogens are associated with lower likelihood of hypertension in adults living in the Mediterranean area. "IndyWatch Feed Health"

PMID:  Molecules. 2018 Feb 9 ;23(2). Epub 2018 Feb 9. PMID: 29425149 Abstract Title:  Dietary Phytoestrogen Intake is Inversely Associated with Hypertension in a Cohort of Adults Living in the Mediterranean Area. Abstract:  BACKGROUND: Dietary polyphenols, including phytoestrogens are abundantly present in a balanced diet. Evidence for their role in preventing non-communicable diseases is emerging.METHODS: We examined the association between estimated habitual intakes of dietary phytoestrogens and hypertension in a cohort study. The baseline data included 1936 men and women aged 18 years and older. Intakes of total phytoestrogens, isoflavones, and lignans were calculated from validated food frequency questionnaire. Data on the polyphenols content in foods were retrieved from the Phenol-Explorer database.RESULTS: Individuals in the highest quartile of dietary phytoestrogens intake were less likely to be hypertensive (OR: 0.66, 95% CI: 0.44-0.98); moreover, the association showed a significant decreasing trend. Isoflavones and lignans were not associated with lower odds of hypertension; however, some individual compounds, such as biochanin A and pinoresinol showed an independent inverse association with hypertension.CONCLUSIONS: Dietary phytoestrogens are associated with lower likelihood of hypertension in adults living in the Mediterranean area. Future studies are needed to confirm the present findings (i.e., prospective cohort studies) and to better understand the mechanisms underlying such associations.

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10:43

Med-Diet adherence might potentially delay the onset of age-related health deterioration and reduce the need of multiple medications. "IndyWatch Feed Health"

PMID:  J Nutr Health Aging. 2018 ;22(1):73-81. PMID: 29300425 Abstract Title:  Aging and Adherence to the Mediterranean Diet: Relationship with Cardiometabolic Disorders and Polypharmacy. Abstract:  OBJECTIVES: To investigate the association between adherence to the Mediterranean Diet (Med-Diet), cardiometabolic disorders and polypharmacy.DESIGN: Cross-sectional study.SETTING: Geriatrics outpatient clinic, Policlinico Umberto I, Sapienza University of Rome.PARTICIPANTS: 508 patients (219 male, 289 female) aged 50 to 89 who were evaluated for cardiovascular and metabolic disorders.METHODS AND MEASUREMENTS: Patients underwent a comprehensive medical assessment including medical history and the use of medications. Adherence to Med-Diet was assessed using the validated Med-Diet 14-item questionnaire; for the analysis, patients were divided in high (8) and medium-low (

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10:28

Pumpkin seed oil prevents the high fat diet induced obesity by altering the markers important to lipid metabolism. "IndyWatch Feed Health"

PMID:  Nat Prod Res. 2017 Oct 19:1-4. Epub 2017 Oct 19. PMID: 29047298 Abstract Title:  Anti obese potential of Cucurbita maxima seeds oil: effect on lipid profile and histoarchitecture in high fat diet induced obese rats. Abstract:  In this study, we made an attempt to evaluate the potential of Cucurbita maxima seeds oil (CSO) against high-fat diet (HFD)-induced obesity in rats. We investigated the effect of CSO (100 mg/kg body weight) supplementation over 30 days on the changes of HFD-induced obese rats in body weight, biochemical parameters and lipid profile as well as investigated the effects of CSO on the histopathological changes. Oral administration with CSO revealed significant diminution in body weight gain, glucose and insulin levels, which altered the activity of lipid profile and restored the pathological alterations. It demonstrated that CSO had considerably altered these parameters when evaluated with HFD control rats. In conclusion, this study established that CSO prevents the HFD-inducedobesity by altering the markers important to lipid metabolism.

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09:54

Dietary supplementation with almonds may have a beneficial effect in reducing the risk of oxidative stress-induced memory loss. "IndyWatch Feed Health"

PMID:  Plant Foods Hum Nutr. 2018 Feb 10. Epub 2018 Feb 10. PMID: 29429075 Abstract Title:  Dietary Supplementation of Almond Prevents Oxidative Stress by Advocating Antioxidants and Attenuates Impaired Aversive Memory in Male Rats. Abstract:  Scopolamine, an anti-muscarinic agent, has been shown to induce amnesia and oxidative stress similar to that observed in the older age. The present study was designed to determine the relationship between the oxidative status and memory improvement in scopolamine injected rats pre-administered with almonds. Rats (n=8) in the almond group were administered orally with 400 mg/kg almond suspension for 28 days daily before the intraperitoneal injection of scopolamine (0.5 mg/kg). Passive avoidance task (PAT) was used to assess memory function at the end of treatment. The present study revealed that scopolamine injection significantly impaired the memory function in rats pre-treated with saline which was accompanied by increased oxidative stress as evident by increased brain malondialdehyde (MDA) levels and reduced activities of antioxidant enzymes as compared to healthy controls. Pre-treatment withalmond significantly ameliorated scopolamine-induced oxidative stress and memory dysfunction. These findings suggest that dietary supplementation with almonds may have a beneficial effect in reducing the risk of oxidative stress-induced memory loss and delaying or preventing the onset of age-related memory impairment.

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08:56

A carotenoid extract from a cultivar of pumpkin triggers nonprotective autophagy in malignant cells. "IndyWatch Feed Health"

PMID:  Oxid Med Cell Longev. 2017 ;2017:7468538. Epub 2017 Dec 21. PMID: 29430284 Abstract Title:  A Carotenoid Extract from a Southern Italian Cultivar of Pumpkin Triggers Nonprotective Autophagy in Malignant Cells. Abstract:  Carotenoids, including-carotene, lycopene, and derivatives, such as retinoic acid, have been studied for their significant antiproliferative and differentiating activity on cancer cells in experimental models and in clinics. We are presenting here data on the mechanism of action of a carotenoid-enriched extract obtained from the pumpkin, variety"long of Naples,"on two malignant human cell lines, Caco-2 and SAOs, derived from a colon adenocarcinoma and an osteosarcoma, respectively. The carotenoid extract has been obtained from pumpkin pulp and seeds by supercritical COextraction and employed to prepare oil-in-water nanoemulsions. The nanoemulsions, applied at a final carotenoid concentration of 200-400g/ml, were not cytotoxic, but induced a delay in cell growth of about 40% in both SAOs and Caco-2 cell lines. This effect was associated with the activation of a"nonprotective"form of autophagy and, in SAOs cells, to the induction of cell differentiation via a mechanism that involved AMPK activation. Our data suggest the presence of a pool of bioactive compounds in the carotenoid-enriched extract, acting additively, or synergistically, to delay cell growth in cancer cells.

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08:44

nuts and peanut butter might reduce pancreatic cancer risk in men. "IndyWatch Feed Health"

PMID:  Cancer Epidemiol Biomarkers Prev. 2018 Jan 22. Epub 2018 Jan 22. PMID: 29358224 Abstract Title:  Total nut, tree nut, peanut, and peanut butter consumption and the risk of pancreatic cancer in the Netherlands Cohort Study. Abstract:  BACKGROUND: Nut intake has been associated with decreased cancer-related mortality, but few studies have examined the potential of nuts in the chemoprevention of pancreatic cancer. We prospectively investigated the association of total nut, tree nut, peanut, and peanut butter consumption with pancreatic cancer risk.METHODS: In the Netherlands Cohort Study, 120,852 men and women completed a baseline questionnaire, including a food frequency questionnaire, in 1986. After 20.3 years of follow-up, 583 incident pancreatic cancer cases, including 349 microscopically confirmed pancreatic cancer (MCPC) cases, were included in multivariable case-cohort analyses.RESULTS: Increased total nut consumption was associated with a non-significantly decreased MCPC risk in men (HR (95% CI) for 10+ g/day vs. nonconsumers = 0.72 (0.47-1.11), P-trend = 0.163). No clear association was found in women. For tree nut and peanut consumption, non-significant inverse associations were observed in men. In women, no or unclear associations were found for tree nut and peanut consumption. Peanut butter intake was related to a significantly reduced risk of MCPC in men (HR (95% CI) for 5+ g/day vs. nonconsumers = 0.53 (0.28-1.00), P-trend = 0.047), but this relation was not clear in women. Evidence for a nonlinear dose-response relation with MCPC was found for tree nut intake only. The associations were weaker when looking at total pancreatic cancer.CONCLUSIONS: Our results suggest that nuts and peanut butter might reduce pancreatic cancer risk in men. In women, no or unclear associations were found.IMPACT: Nut consumption might reduce the risk of pancreatic cancer in men.

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08:40

These results confirm the effects of lipid-lowering and modifying body composition of almond consumption. "IndyWatch Feed Health"

PMID:  J Med Food. 2018 Jan 9. Epub 2018 Jan 9. PMID: 29315019 Abstract Title:  Time and Intervention Effects of Daily Almond Intake on the Changes of Lipid Profile and Body Composition Among Free-Living Healthy Adults. Abstract:  Favorable health benefits of almond have been shown in several previous studies. However, repeated measures, randomized, controlled trials to investigate the changes due to almond intake based on the time effects have not yet been reported. The current study was conducted to evaluate the effects of daily almond intake on changes in body composition and lipid profiles for 20 weeks with four measurements among healthy adults. Participants in the almond group showed favorable changes on blood lipid profiles, including levels of triglycerides (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), and non-high-density lipoprotein (non-HDL-C) after consuming 56g of almond per day for 20 weeks compared with those at baseline. At week 20, subjects in the almond group showed significantly decreased TC, LDL-C, non-HDL-C, TG, body fat mass, and waist-hip ratio compared with those of the control group who consumed isocaloric control food. The mixed model also confirmed that there were significant time effects in several bioimpedance indicators (i.e., total body protein, fat-free mass, etc.) and all of the lipid profile parameters in the almond group. These results confirm the effects of lipid-lowering and modifying body composition of almond consumption. In addition, our results suggest that the measuring time points would be critical to capture the effects of dietary intervention.

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08:34

These results reveal that almond consumption induced changes in the microbial community composition of the human gastrointestinal microbiota. "IndyWatch Feed Health"

PMID:  Nutrients. 2018 Jan 26 ;10(2). Epub 2018 Jan 26. PMID: 29373513 Abstract Title:  Almond Consumption and Processing Affects the Composition of the Gastrointestinal Microbiota of Healthy Adult Men and Women: A Randomized Controlled Trial. Abstract:  BACKGROUND: Almond processing has been shown to differentially impact metabolizable energy; however, the effect of food form on the gastrointestinal microbiota is under-investigated.OBJECTIVE: We aimed to assess the interrelationship of almond consumption and processing on the gastrointestinal microbiota.DESIGN: A controlled-feeding, randomized, five-period, crossover study with washouts between diet periods was conducted in healthy adults (= 18). Treatments included: (1) zero servings/day of almonds (control); (2) 1.5 servings (42 g)/day of whole almonds; (3) 1.5 servings/day of whole, roasted almonds; (4) 1.5 servings/day of roasted, chopped almonds; and (5) 1.5 servings/day of almond butter. Fecal samples were collected at the end of each three-week diet period.RESULTS: Almond consumption increased the relative abundances of,, and( 0.05). Comparisons between control and the four almond treatments revealed that chopped almonds increased,, andcompared to control (

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08:12

Cashew nut supplementation in Asian Indians with T2DM reduced systolic blood pressure and increased HDL cholesterol concentrations. "IndyWatch Feed Health"

PMID:  J Nutr. 2018 Jan 1 ;148(1):63-69. PMID: 29378038 Abstract Title:  Cashew Nut Consumption Increases HDL Cholesterol and Reduces Systolic Blood Pressure in Asian Indians with Type 2 Diabetes: A 12-Week Randomized Controlled Trial. Abstract:  Background: There is increasing evidence that nut consumption decreases the risk of cardiovascular disease. However, there are few data on the health effects of cashew nuts among adults with type 2 diabetes (T2DM).Objective: The study aimed to investigate the effects of cashew nut supplementation on glycemia, body weight, blood pressure, and lipid profile in Asian Indians with T2DM.Methods: In a parallel-arm, randomized controlled trial, 300 adults with T2DM [mean SD age: 51 9.3 y; body mass index (BMI; in kg/m2): 26.0 3.4; 55% male] were randomly assigned to receive advice to follow a standard diabetic diet (control) or similar advice plus 30 g cashew nuts/d (intervention) for 12 wk. The macronutrient composition of the prescribed diabetic diet was60-65% energy from carbohydrates, 15-25% from fat, and the rest from protein. Differences between groups in changes in anthropometric and biochemical variables were analyzed using linear models with robust variance estimation under an assumed independence working correlation.Results: Participants in the intervention group had a greater decrease in systolic blood pressure from baseline to 12 wk than did controls (-4.9 13.7 compared with -1.7 11.6 mm Hg; P = 0.04) and a greater increase in plasma HDL cholesterol compared with controls (+1.7 5.6 compared with +0.1 4.6 mg/dL; P = 0.01). There were no differences between the groups with respect to changes in body weight, BMI, blood lipid, and glycemic variables. Plasma oleic acid concentrations and self-reported dietary intake of nuts, oleic acid, and monounsaturated fatty acids suggested excellent compliance with the nut consumption.Conclusion: Cashew nut supplementation in Asian Indians with T2DM reduced systolic blood pressure and increased HDL cholesterol concentrations with no deleterious effects on body weight, glycemia, or other lipid variables. This study was registered at the clinical trial registry of India as CTRI/2017/07/009022.

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08:03

The findings indicate that the nut consumption may contribute to the delay of cognitive decline in aging. "IndyWatch Feed Health"

PMID:  Curr Alzheimer Res. 2018 Feb 1. Epub 2018 Feb 1. PMID: 29422003 Abstract Title:  Role of Nut Consumption in the Management of Cognitive Decline - A Mini-Review. Abstract:  BACKGROUND: Currently, there is a significant increase in the number of older generation groups, which may result in serious economic and social issues. Therefore there is a need to prolong the active life of these older individuals, especially by focusing on modifying lifestyle factors such as healthy nutrition. In fact, recent research has shown that, for example, nuts are an important part of people's healthy diet because they have appeared to be neuroprotective compounds which might maintain or in some cases even improve people's cognitive functions.OBJECTIVE: The purpose of this review study is to explore the role of the nut nutrition in the maintenance and delay of cognitive decline among older individuals.RESULTS: The findings indicate that the nut consumption may contribute to the delay of cognitive decline in aging. However, this nut diet is just one component of the multi-nutrient dietary intervention for health aging.CONCLUSION: More longitudinal controlled randomized studies have to be performed in this field to prove the efficacy of the nut nutrition for the delay of cognitive decline.

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07:29

Nephroprotection of plantamajoside in rats treated with cadmium. "IndyWatch Feed Health"

PMID:  Environ Toxicol Pharmacol. 2015 Jan ;39(1):125-36. Epub 2014 Nov 30. PMID: 25499790 Abstract Title:  Nephroprotection of plantamajoside in rats treated with cadmium. Abstract:  Cadmium (Cd), an environmental and industrial pollutant, generates free radicals responsible for oxidative stress. Cd can also lead to various renal toxic damage such as the proximal tubules and glomerulus dysfunction. Plantamajoside (PMS), a major compound of Plantago asiatica (PA), was reported to have the antioxidant effects. In this study, we investigated the protective effects of PMS on Cd-induced renal damage in the NRK-52E cell and rat kidney tissue. Cd exposure increased the ROS generation, lipid peroxidation, serum biochemical values of renal damage, and mRNA and protein expressions of KIM-1 in vitro and in vivo. The significant reduction in glutathione (GSH)/glutathione disulfide (GSSG) ratio and activities of antioxidant enzymes were also observed in the rats treated with Cd. PMS significantly decreased the ROS generation and lipid peroxidation, thus enhancing GSH/GSSG ratio, antioxidant enzyme activities in the cells and rats, and improved histochemical appearances, indicating that PMS has protective activities against Cd-induced renal injury.

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07:16

Plantamajoside inhibits UVB and advanced glycation end products-induced MMP-1 expression. "IndyWatch Feed Health"

PMID:  Photochem Photobiol. 2016 09 ;92(5):708-19. Epub 2016 Aug 9. PMID: 27346084 Abstract Title:  Plantamajoside Inhibits UVB and Advanced Glycation End Products-Induced MMP-1 Expression by Suppressing the MAPK and NF-B Pathways in HaCaT Cells. Abstract:  Photoaging and glycation stress are major causes of skin deterioration. Oxidative stress caused by ultraviolet B (UVB) irradiation can upregulate matrix metalloprotease 1 (MMP-1), a major enzyme responsible for collagen damage in the skin. Advanced glycation end products (AGEs) accumulate via gradual formation from skin proteins, especially from long-lived proteins such as dermal elastin and collagen. Plantamajoside (PM), isolated from Plantago asiatica, has various biological effects including anti-inflammatory and antioxidant effects. In this study, we assessed the protective effects of PM on a human keratinocyte cell line (HaCaT) and primary human dermal fibroblasts (HDF) against stress caused by glyceraldehyde-induced AGEs (glycer-AGEs) with UVB irradiation. We found that PM attenuated UVB- and-glycer-AGEs-induced MMP-1 expression in HaCaT and HDF cells and proinflammatory cytokines expression by inhibiting the phosphorylation of mitogen-activated protein kinases (MAPKs) activated by reactive oxygen species. Specific inhibitors of NF-B and MAPKs attenuated the induced expression of MMP-1. PM also inhibited the phosphorylation of IB, and reduced nuclear translocation of NF-B in these cells. Furthermore, PM attenuated the upregulation of receptor for AGEs (RAGE) by glycer-AGEs with UVB irradiation. Therefore, our findingsstrongly suggest that PM is a promising inhibitor of skin photoaging.

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07:01

Antioxidant-rich extract from plantaginis semen Ameliorates diabetic retinal injury in a streptozotocin-induced diabetic rat model. "IndyWatch Feed Health"

PMID:  Nutrients. 2016 Sep 18 ;8(9). Epub 2016 Sep 18. PMID: 27649243 Abstract Title:  Antioxidant-Rich Extract from Plantaginis Semen Ameliorates Diabetic Retinal Injury in a Streptozotocin-Induced Diabetic Rat Model. Abstract:  Plantaginis semen, the dried ripe seed of Plantago asiatica L. or Plantago depressa Willd. (Plantaginaceae), has been traditionally used to treat blurred vision in Asia. The aim of this work was to investigate the effect of plantaginis semen ethanol extract (PSEE) on the amelioration of diabetic retinopathy (DR) in streptozotocin (STZ)-diabetic rats. PSEE has abundant polyphenols with strong antioxidant activity. PSEE (100, 200 or 300 mg/kg) was oral administrated to the diabetic rats once daily consecutively for 8 weeks. Oral administration of PSEE resulted in significant reduction of hyperglycemia, the diameter of the retinal vessels, and retinal vascular permeability and leukostasis in diabetic rats. In addition, PSEE administration increased the activities of superoxidase dismutase (SOD) and catalase (CAT), and glutathione peroxidase (GSH) level in diabetic retinae. PSEE treatment inhibited the expression of vascular endothelial growth factor (VEGF) and hypoxia-inducible factor-1 (HIF-1) and the phosphorylation of Akt without altering the Akt protein expression in diabetic retinae. PSEE not only down-regulated the gene expression of hypoxia-inducible factor-1 (TNF-) and interleukin-1 (IL-1), but also reduced ICAM-1 and VCAM-1 expression in diabetic retinae. Moreover, PSEE reduced the nuclear factor-B (NF-B) activation and corrected imbalance between histone deacetylases (HDAC) and histone acetyltransferases (HAT) activities in diabetic retinae. In conclusion, phenolic antioxidants extract from plantaginis semen has potential benefits in the preventionand/or progression of DR.

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07:00

Do Your Moles Ever Go Away? "IndyWatch Feed Health"

By Dr. Mercola

Skin is your body's largest and fastest growing organ, used to protect your body from bacteria and germs, regulate your temperature, get rid of waste products and house a nervous system that allows you to feel and sense your environment.1 Your skin is what others first notice about you, and is an indicator of both internal and external aging.

Skin cancer is one of the most common types of cancer in the U.S.2 Estimates suggest nearly 20 percent of all Americans will experience some form of skin cancer during their life. Non-melanoma skin cancers affect nearly 3 million Americans each year and more than 1 million are living with melanoma. The rates of diagnosis of basal cell (BCC) and squamous cell carcinomas (SCC), non-melanoma skin cancers, have been rising dramatically.

There has been an increase of 145 percent of BCC between 1976 to 1984 and 2000 to 2010, and a 263 percent increase of SCC during the same period.3 Routinely inspecting your skin increases the likelihood you'll recognize changes that may need medical attention, and will help you become more familiar with your unique pattern of moles (nevi), freckles and blemishes.

Monthly self-inspection is done in a well-lit room with the assistance of mirrors to inspect areas that are difficult to see. Your first examination may take longer as you record the location of moles and freckles on your body. This enables you to confirm the location and any changes each month forward. You might notice that a mole suddenly appears where there wasn't one before, or a mole you've had for years may start to disappear.

Evolution of a Mole

Moles are growths on your skin that are usually black or brown, but can be a lighter tan. They usually appear in childhood anywhere on your body, either alone or in groups. By the time you reach your mid-20s it's common to have 10 to 40 moles.4 Moles happen when cells that provide color to your skin melanocytes grow in clumps together instead of spreading throughout your skin. After being exposed to the sun or during hormonal changes, such as your teen years or pregnancy, moles may darken in color.

Not all moles are created equally. The common mole is 5 to 6 mm in diameter...

The Motivation Factor "IndyWatch Feed Health"

By Dr. Mercola

Research1 has convincingly demonstrated that the more physically active children and teenagers are, the better they do academically. As noted by the authors of a 2012 scientific review:

"Physical activity and sports are generally promoted for their positive effect on children's physical health There is also a growing body of literature suggesting that physical activity has beneficial effects on several mental health outcomes In addition ... there is a strong belief that regular participation in physical activity is linked to enhancement of brain function and cognition, thereby positively influencing academic performance."

Several mechanisms that help explain why physical activity benefits cognition have been proposed. For starters, exercise increases blood and oxygen flow to your brain, which in and of itself increases learning speed.2 Exercise also increases levels of norepinephrine and endorphins, which lowers stress and improves mood. Importantly, exercise triggers growth factors that create new nerve cells and enable brain plasticity, thereby facilitating memory and learning. According to the authors of this 2012 review:

"The increasing pressures to improve academic scores often lead to additional instructional time for subjects such as mathematics and language at the cost of time for being physically active [T]he literature provides inconclusive evidence on the positive longitudinal relationship between physical activity and academic performance. However, there is a strong general belief that this relationship is present, and research in this area is ongoing."

The Motivation Factor

The featured documentary, "The Motivation Factor,"3 investigates these claims, showing how exercise and particularly physical education (PE) in school helps motivate kids and young adults to excel in other areas of life, including academically.

"The knowledge that the physical well-being of the citizen is an important foundation for the vigor and vitality of all the activities of the nation, is as old as the Western civiliz...

06:49

Plantago asiatica seed extract improves lipid accumulation and hyperglycemia in high-fat diet-induced obese mice. "IndyWatch Feed Health"

PMID:  Int J Mol Sci. 2017 Jun 30 ;18(7). Epub 2017 Jun 30. PMID: 28665305 Abstract Title:  Plantago asiatica L. Seed Extract Improves Lipid Accumulation and Hyperglycemia in High-Fat Diet-Induced Obese Mice. Abstract:  Obesity and its common association with type 2 diabetes, dyslipidemia, and cardiovascular diseases are worldwide epidemics. Currently, to prevent or treat obesity and associated metabolic disorders, herbal dietary supplements or medicines have attracted more and more attention owing to their relative effectiveness with fewer significant side effects. We investigate the therapeutic effects and underlying mechanisms ofL. seed extract (PSE) on obesity and associated metabolic disorders in high-fat (HF) diet-induced mice. Our results displayed that PSE did not modify food intake or body weight but decreased abdominal white adipose tissue ratio, white/brown adipocyte size, serum total cholesterol, triglyceride (TG), low density lipoprotein cholesterol, free fatty acid, and hepatic TG concentrations when compared with the HF group. The levels of fasting blood glucose and glucose tolerance were improved in the PSE group when compared with the HF group. Furthermore, PSE upregulated mRNA expressions of peroxisome proliferator activated receptors (PPARs) and target genes related to fatty acid metabolism and energy expenditure in liver and adipose tissue of obese mice when compared with the HF group. PSE treatment effectively improved lipid and glucose metabolism in HF diet-induced obese mice. These effects might be attributed to the upregulation of PPAR signaling.

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06:33

Molecular properties and immunomodulatory activities of a water-soluble heteropolysaccharide isolated from Plantago asiatica. "IndyWatch Feed Health"

PMID:  Nat Prod Res. 2018 Jan 31:1-4. Epub 2018 Jan 31. PMID: 29385853 Abstract Title:  Molecular properties and immunomodulatory activities of a water-soluble heteropolysaccharide isolated from Plantago asiatica L. leaves. Abstract:  This study aims at investigating physical-chemical properties of a water-soluble heteropolysaccharide from Plnatago asiatica L. leaves, and its immunomodulatory effects on macrophages RAW264.7 cells. Hot water-extracted crude polysaccharide from the plant leaves (PLLCP) was fractionated into four fractions by DEAE Sepharose Fast Flow eluted with 0.1-0.5 M NaCl solutions. The most abundant fraction (0.3 M NaCl elution), designated PLLP-2, was identified as a heteropolysaccharide with an average molecular weight (M) of 3.54  10and composed mainly of Gal (34.4%), GalA (36.5%), Ara (10.1%) and Rha (8.4%). PLLP-2 was an acidic polysaccharide exhibiting inflaky curly aggregation with smooth surface. PLLCP and its main subfraction PLLP-2 displayed immunomodulatory activities by stimulating the production of pro-inflammatory cytokines, including TNF- and IL-1 in macrophage RAW264.7 cells. These results indicate that the main polysaccharide fraction purified from P. asiatica L. leaves is probably pectin, and have potential immunomodulatory function.

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06:27

A. vera extract exhibited pancreatic islet cell rejuvenation. "IndyWatch Feed Health"

PMID:  Pharmacognosy Res. 2017 Dec ;9(Suppl 1):S99-S104. PMID: 29333050 Abstract Title:  Improvement of Insulin Secretion and Pancreatic-cell Function in Streptozotocin-induced Diabetic Rats Treated withExtract. Abstract:  Background: Diabetes mellitus is a metabolic disorder characterized by chronic hyperglycemia. Plant extracts and their products are being used as an alternative system of medicine for the treatment of diabetes.has been traditionally used to treat several diseases and it exhibits antioxidant, anti-inflammatory, and wound-healing effects. Streptozotocin (STZ)-induced Wistar diabetic rats were used in this study to understand the potential protective effect ofextract on the pancreatic islets.Objective: The aim of the present study was to evaluate theextract on improvement of insulin secretion and pancreatic-cell function by morphometric analysis of pancreatic islets in STZ-induced diabetic Wistar rats.Materials and Methods: After acclimatization, male Wistar rats, maintained as per the Committee for the Purpose of Control and Supervision of Experiments on Animals guidelines, were randomly divided into four groups of six rats each. Fasting plasma glucose and insulin levels were assessed. The effect ofextract in STZ-induced diabetic rats on the pancreatic islets by morphometric analysis was evaluated.Results: Oral administration ofextract (300 mg/kg) daily to diabetic rats for 3 weeks showed restoration of blood glucose levels to normal levels with a concomitant increase in insulin levels upon feeding withextract in STZ-induced diabetic rats. Morphometric analysis of pancreatic sections revealed quantitative and qualitative gain in terms of number, diameter, volume, and area of the pancreatic islets of diabetic rats treated withextract when compared to the untreated diabetic rats.Conclusion: extract exerts antidiabetic effects by improving insulin secretion and pancreatic-cell function by restoring pancreatic islet mass in STZ-induced diabetic Wistar rats.SUMMARY: Fasting plasma glucose (FPG) and insulin levels were restored to normal levels in diabetic rats treated withextractIslets of pancreas were qualitatively and quantitatively restored to normalcy leading to restoration of FPG and insulin levels of diabetic rats treated withextractMorphometric analysis of pancreatic sections revealed quantitative and qualitative gain in terms of number, diameter, volume, and area of the pancreatic islets of diabetic rats treated withextract when compared to the untreated diabetic rats., FPG: Fasting plasma glucose, STZ: Streptozotocin, BW: Body weight.

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06:17

The results of this study support the existing data and the empirical uses of P. major. "IndyWatch Feed Health"

PMID:  Pharmacogn Mag. 2017 Jul-Sep;13(51):393-399. Epub 2017 Jul 19. PMID: 28839362 Abstract Title:  Effects ofExtracts and Its Chemical Compounds on Proliferation of Cancer Cells and Cytokines Production of Lipopolysaccharide-activated THP-1 Macrophages. Abstract:  BACKGROUND: has been reported to have anticancer and anti-inflammatory properties. However, its antiproliferative and anti-inflammatory mechanisms have not been fully elucidated. Moreover, which plant parts are more suitable as starting materials has not been explored.OBJECTIVES: To investigate the antiproliferative activity ofextracts against MCF-7, MDA-MB-231, HeLaS3, A549, and KB cancer cell lines as well as their effects on inflammatory cytokines (tumor necrosis factor [TNF]-, interleukin [IL]-1, IL-6, and interferon [IFN]-) production by lipopolysaccharide (LPS)-stimulated THP-1 macrophages.MATERIALS AND METHODS: The methanol and aqueous extracts offrom different plant parts and its chemical compounds, i.e., ursolic acid (UA), oleanolic acid (OA), and aucubin were tested in this experiment.RESULTS: Methanol and aqueous extracts ofseeds exhibited the greatest antiproliferative activity. The methanol extracts of seeds also demonstrated the highest inhibition of TNF-, IL-1, IL-6, and IFN- production. Interestingly, the roots, which were commonly discarded, exhibited comparable activities to those of leaves and petioles. Furthermore, UA exhibited stronger activities than OA and aucubin.CONCLUSIONS: The seeds are being proposed as the main source for further development of anticancer and anti-inflammatory products, whereas the roots could be included in the preparation ofderived products with respect to anti-inflammatory.SUMMARY: Amongst the parts of, seeds exhibited the greatest antiproliferative activity against MCF-7, MDA-MB-231, HeLaS3, A549, and KB cell lines as well as the highest inhibition on TNF-, IL-1, IL-6, and IFN- productionThe roots, which were commonly discarded, exhibited comparable antiproliferative and cytokines inhibition activities to those of leaves and petiolesUrsolic acid, a chemical compound of, exhibited stronger activities than oleanolic acid and aucubinThe seeds are being proposed as the main source for further development of anticancer and anti inflammatory products, whereas the roots could be included in the preparation ofderived products with respect to anti inflammatory.TNF: Tumor Necrosis Factor; IL: Interleukin; IFN: Interferon; HPTLC: High Performance Thin Layer Chromatography; UA: Ursolic Acid; OA: Oleanolic Acid; AUC: Aucubin.

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06:07

Chemical constituents and medical benefits of Plantago major. "IndyWatch Feed Health"

PMID:  Biomed Pharmacother. 2017 Dec ;96:348-360. Epub 2017 Oct 10. PMID: 29028587 Abstract Title:  Chemical constituents and medical benefits of Plantago major. Abstract:  The medicinal benefits of Plantago major have been acknowledged around the world for hundreds of years. This plant contains a number of effective chemical constituents including flavonoids, alkaloids, terpenoids, phenolic acid derivatives, iridoid glycosides, fatty acids, polysaccharides and vitamins which contribute to its exerting specific therapeutic effects. Correspondingly, studies have found that Plantago major is effective as a wound healer, as well as an antiulcerative, antidiabetic, antidiarrhoeal, anti-inflammatory, antinociceptive, antibacterial, and antiviral agent. It also combats fatigue and cancer, is an antioxidant and a free radical scavenger. This paper provides a review of the medicinal benefits and chemical constituents of Plantago major published in journals from year 1937 to 2015 which are available from PubMed, ScienceDirect and Google Scholar.

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05:52

Topical Plantavera gel seems to be an effective, cheap and safe treatment of diabetic foot ulcer. "IndyWatch Feed Health"

PMID:  Curr Drug Discov Technol. 2018 01 14. Epub 2018 Jan 14. PMID: 29336265 Abstract Title:  Efficacy of Aloe vera/ Plantago major gel in Diabetic Foot Ulcer: a randomized double-blind clinical trial. Abstract:  BACKGROUND: Diabetic foot ulcer (DFU) is one of the most common complications of diabetic patients. Mostly, non-healing DFU leads to infection, gangrene, amputation and even death. High costs and poor healing of the wounds need a new treatment such as alternative medicine. So, the aim of this study was to evaluate the efficacy of Aloe vera/ Plantago major gel (Plantavera gel) in healing of DFU.METHODS: Forty patients with DFU enrolled in a double-blind randomized clinical trial. The patients who were randomly assigned into the intervention group (n=20), received topical Plantavera gel in addition to the routine cares, whereas the patients in the control group (n=20), received topical Placebo gel in addition to the routine cares. Intervention was done twice a day for 4 weeks in the both groups. Photography and an evaluation of DFUhealing were conducted by a checklist and then were scored at baseline and at the end of each week. The collected data was analyzed by SPSS software.RESULTS: At the end of the study, there was a significant difference between the two groups in terms of total ulcer score (P

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05:34

Thymol can be therapeutic against high risk factors for Alzheimer's disease. "IndyWatch Feed Health"

PMID:  Brain Res Bull. 2018 Jan 13 ;137:338-350. Epub 2018 Jan 13. PMID: 29339105 Abstract Title:  Effects of thymol on amyloid--induced impairments in hippocampal synaptic plasticity in rats fed a high-fat diet. Abstract:  Obesity and a high-fat diet (HFD) are known to increase the incidence of Alzheimer's disease (AD). Oxidative stress, a major risk factor for AD, is increased with HFD consumption. Thymol (Thy) has antioxidant properties. Therefore, in the present study, we examined the protective and therapeutic effects of Thy on amyloid- (A)-induced impairments in the hippocampal synaptic plasticity of HFD-fed rats. In this study, 72 adult male Wistar rats were randomly assigned to 9 groups (n=8 rats/group): Group 1 (control; standard diet); Group 2: Control+phosphate-buffered saline (PBS)+Oil (Thy vehicle); Group 3 (HFD+PBS); Group 4: (HFD+A); Group 5: Control+PBS+Thy; Group 6: (HFD+A+Oil); Group 7: Control+A+Thy; Group 8: HFD+PBS+Thy; Group 9: (HFD+A+Thy). After stereotaxic surgery, the field potentials were recorded afterthe implantation of the recording and stimulating electrodes in the dentate gyrus (DG) and perforant pathway, respectively. Following high-frequency stimulation, the long-term potentiation (LTP) of the population spike (PS) amplitude and the slope of the excitatory postsynaptic potentials (EPSPs) were measured in the DG. The HFD rats that received A exhibited a significant decrease in their EPSP slope and PS amplitude as compared to the control group. In contrast, Thy administration in the HFD+A rats reduced the decrease in the EPSP slope and PS amplitude. Thy decreased the A-induced LTP impairments in HFD rats. The HFD significantly increased serum malondialdehyde levels and decreased total antioxidant capacity and total glutathione levels; whereas, Thy supplementation significantly reversed these parameters. Therefore, these results suggest that Thy, a natural antioxidant,can be therapeutic against high risk factors for AD, such as HFD.

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05:27

Thymoquinone reverses learning and memory impairments and brain tissue oxidative damage in hypothyroid juvenile rats. "IndyWatch Feed Health"

PMID:  Arq Neuropsiquiatr. 2018 Jan ;76(1):32-40. PMID: 29364392 Abstract Title:  Thymoquinone reverses learning and memory impairments and brain tissue oxidative damage in hypothyroid juvenile rats. Abstract:  In this study, the effect of thymoquinone (TQ) on propylthiouracil (PTU)-induced memory impairment was investigated in juvenile rats. The rats were grouped into control, Hypo, Hypo-TQ5 and Hypo-TQ10. Propylthiouracil increased latency time in the Morris water maze test and decreased delay in entering the dark compartment in the passive avoidance test. Both 5 mg/kg and 10 mg/kg doses of TQ decreased latency time in the Morris water maze test and increased delay in entering the dark compartment in a passive avoidance test. The PTU also increased malondialdehyde and nitric oxide metabolites in the brain while reduced the thiol content and superoxide dismutase and catalase activities and serum T4 level. Both doses of TQ decreased malondialdehyde and nitric oxide metabolites in the brain while enhanced the thiol content and superoxide dismutase and catalase activities and serum T4 level. The results of the present study showed that TQ protected against PTU-induced memory impairments in rats.

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05:20

Thymoquinone activates MAPK pathway in hippocampus of streptozotocin-treated rat model. "IndyWatch Feed Health"

PMID:  Biomed Pharmacother. 2018 Jan 20 ;99:391-401. Epub 2018 Jan 20. PMID: 29367108 Abstract Title:  Thymoquinone activates MAPK pathway in hippocampus of streptozotocin-treated rat model. Abstract:  Streptozotocin (STZ), a glucosamine-nitrosourea compound, produces deficiencies in learning, memory, and cognitive functions when it was administered intracerebroventricularly (i.c.v). In molecular level, increase in neuroinflammation and oxidative stress in brain, and decrease in the number of surviving neurons are the outcomes of STZ administration. Herein, we aimed to investigate the effect of thymoquinone (TQ), an anti-inflammatory, immunomodulatory and neuroprotective agent, on STZ-induced neurodegeneration in rats. For this purpose, bilateral i.c.v. injection of STZ (3mg/kg) was given to adult female rats on days 1 and 3. TQ (20mg/kg/day in cornoil) was administered intragastrically to rats for 15 days starting from the 15th day of STZ injection. The Morris water maze test and passive avoidance test were applied to measure the learning and memory performance of animals. Following the behavioral tests, all of the rats were sacrificed for evaluation of molecular alterations. Rats in the STZ-TQ group showed higher performance in passive avoidance test than rats in the STZ group whose memory performance declined compared to control group. The worse memoryperformance in STZ group was correlated with low number of surviving neurons and high number of degenerating neurons. In addition, an increase in APOE expression and a decrease in NGF expression were observed with STZ injection. Administration of TQ reversed these STZ-triggered cognitive and molecular alterations. In the present study, we observed the neuroregenerative effects of TQ by activation of JNK protein, upregulation of mir-124, and downregulation of ERK1/2 and NOS enzymes. The same ameliorative effect of TQ was also observed in the pTau protein expression. To sum up, we can say thatthe healing effect of TQ on STZ induced neurodegeneration opens a new door for the development of Alzheimer's disease treatment using natural products as an adjuvant when their action mechanism was explained in detail.

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05:14

Thymoquinone alleviates the experimentally induced Alzheimer's disease inflammation. "IndyWatch Feed Health"

PMID:  Hum Exp Toxicol. 2018 Jan 1:960327118755256. Epub 2018 Jan 1. PMID: 29405769 Abstract Title:  Thymoquinone alleviates the experimentally induced Alzheimer's disease inflammation by modulation of TLRs signaling. Abstract:  Alzheimer's disease (AD) is characterized by a robust inflammatory response elicited by the accumulation and deposition of amyloid- (A) within the brain. A induces detrimental inflammatory responses through toll-like receptors (TLRs) signaling pathway. Thymoquinone (TQ), the main active constituent of Nigella sativa oil, has been reported by several previous studies for its potent anti-inflammatory effect. The aim of thisstudy is to elucidate the effect of TQ in improving learning and memory, using a rat model of AD induced by a combination of aluminum chloride (AlCl) and d-galactose (d-Gal). TQ was administered orally at doses of 10, 20, and 40 mg/kg/day for 14 days after AD induction. Memory functions were assessed using the step through passive avoidance test. Amyloid plaques were shown to be present using hematoxylin and eosin staining. Tumor necrosis factor-alpha (TNF-) and Interleukin-1beta (IL-1) levels in brain were assessed via ELISA and profiling TLR-2, TLR-4, myeloid differential factor 88, toll-interleukin-1 receptor domain-containing adapter-inducing interferon-, interferon regulatory factor 3 (IRF-3), and nuclear factor-B (NF-B) expressions viareal-time polymerase chain reaction. TQ improved AD rat cognitive decline, decreased A formation and accumulation, significantly decreased TNF- and IL-1 at all levels of doses and significantly downregulated the expression of TLRs pathway components as well as their downstream effectors NF-B and IRF-3 mRNAs at all levels of doses ( p

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05:14

Prevention Against Gallstones - Eat More Foods With Vitamin E "IndyWatch Feed Health"

More than 90 percent of the population does not meet their recommended daily allowance of vitamin E. Researchers in Germany led by the University of Kiel found that subjects with gallstone disease had lower blood levels of vitamin E than those who did not have gallstones.

05:09

Immunomodulatory and anti-inflammatory effects of thymoquinone. "IndyWatch Feed Health"

PMID:  Cardiovasc Hematol Disord Drug Targets. 2018 Feb 11. Epub 2018 Feb 11. PMID: 29437018 Abstract Title:  Immunomodulatory and Anti-inflammatory Effects of Thymoquinone. Abstract:  The immunomodulatory properties of Nigella sativa seed, as a basis of its clinical applications by many cultures have been reviewed. Thymoquinone (TQ) is one of the major bioactive component of the volatile oil of N. sativa seeds. The present investigations provide a comprehensive review of the ability of TQ to modulate inflammation and immune-related disorders in the various important disease states. This finding showed that TQ has the anti-inflammatory properties that prevents the biosynthesis of important mediators in inflammatory processes and asthma such as 5-LO, COX, PGD2 and LTs. TQ also reduced LPS-induced proinflammatory cytokines such as interleukins (ILs) and TNF-.. In addition, TQ showed the immunomodulatory role in the cellular and humoral immunity. This study also found that TQ improves the imidacloprid toxicity through reducing oxidative stress and increasing chemokinesis, chemotaxis, phagocytic activity, antibody levels and the hemagglutination of immunoglobulins as well as by reducing serum MDA levels and hepatic enzymes.

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05:02

antioxidant effect of thymoquinone could protect damage of liver parameters against morphine toxicity. "IndyWatch Feed Health"

PMID:  Int J Prev Med. 2018 ;9:8. Epub 2018 Jan 29. PMID: 29441185 Abstract Title:  The Effects of Thymoquinone Against Morphine-induced Damages on Male Mice Liver. Abstract:  Background: Morphine is a pain medication. It is mostly processed in liver and reasons disturbing effects. It can increase the production of free radicals. Thymoquinone is a phytochemical compound found in the plant. It has diverse pharmacological properties such as antioxidant and anticancer. This study was intended to assess the effects of thymoquinone against morphine damages on the liver of mice.Methods: In this study, various doses of thymoquinone (4.5, 9, and 18 mg/kg) and thymoquinone plus morphine was administered (once a day) intraperitoneally to 48 male mice for 20 consequent days. These mice were randomly assigned to eight groups (= 6). Aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, serum nitric oxide (NO) levels, liver weight, and histology have been studied.Results: The results indicated that morphine administration significantly increased the mean diameter of central hepatic vein and hepatocyte, blood serum NO level, liver enzymes level, and decreased liver weight compared to saline group (

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04:48

Xanthohumol protects morphology and function in a mouse model of retinal degeneration. "IndyWatch Feed Health"

PMID:  Invest Ophthalmol Vis Sci. 2018 Jan 1 ;59(1):45-53. PMID: 29305606 Abstract Title:  Xanthohumol Protects Morphology and Function in a Mouse Model of Retinal Degeneration. Abstract:  Purpose: To investigate whether treatment with xanthohumol (XN), the principal prenylated chalconoid from Humulus lupulus (hops), is protective in a mouse model of light-induced retinal degeneration (LIRD).Methods: Mice (129S2/SvPasCrl) were intraperitoneally injected with vehicle or XN prior to toxic light exposure and every 3 days thereafter. Retinal function was assessed by electroretinograms at 1, 2, and 4 weeks following toxic light exposure. Visual acuity was tested by optokinetic tracking 1 week and 4 weeks after toxic light exposure. Retina sections were stained with hematoxylin and eosin for morphologic analysis or by TUNEL. Redox potentials were assessed in retinal tissue by measuring levels of cysteine (CYS), cystine (CYSS), glutathione (GSH), and glutathione disulfide (GSSG) using HPLC with fluorescence detection.Results: Toxic light significantly suppressed retinal function and visual acuity, severely disrupted the photoreceptor cell layer, and significantly decreased the number of nuclei and increased the accumulation of TUNEL-labeled cells in the outer nuclear layer. These effects were prevented by XN treatment. Treatment with XN also maintained GSSG and CYSS redox potentials and the total CYS pool in retinas of mice undergoing toxic light exposure.Conclusions: XN treatment partially preserved visual acuity and retinal function in the LIRD mouse. Preservation of retinal CYS and of GSSG and CYSS redox potentials may indicate that XN treatment induces an increased antioxidant response, but further experiments are needed to verify this potential mechanism. To our knowledge, this is the first study to report protective effects of XN in a model of retinal degeneration.

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04:38

Antibiofilm activity of bioactive hop compounds toward susceptible and resistant staphylococci. "IndyWatch Feed Health"

PMID:  Res Microbiol. 2018 Jan 30. Epub 2018 Jan 30. PMID: 29407045 Abstract Title:  Antibiofilm activity of bioactive hop compounds humulone, lupulone and xanthohumol toward susceptible and resistant staphylococci. Abstract:  Bacterial biofilms pose a serious medical problem due to their significant resistance to antimicrobials, and staphylococci are recognized as the most frequent cause of biofilm-associated infections. The hop plant (Humulus lupulus L.) contains substances that have been determined to act as anti-infective agents against bacteria, mainly in planktonic form. Therefore, we decided to investigate the antibiofilm properties of Humulus lupulus L.-derived compounds (humulone, lupulone and xanthohumol) against a selected group of Staphylococcus spp., including methicillin-susceptible and resistant strains. All tested hop compounds were shown to possess antimicrobial properties against all tested staphylococci, both planktonic and biofilm-dwelling, with no significant difference between resistant and susceptible strains. All compounds lowered the number of bacterial cells released from the biofilm, with the strongest effect seen for lupulone, followed by xanthohumol. Moreover, lupulone and xanthohumol were not only able to penetrate the biofilm and reduce the number of bacteria within it, but their higher concentrations (60 g/mL for xanthohumol and 125 g/mL for lupulone) reduced the number of surviving bacterial cells to zero.

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04:27

Astaxanthin improves glucose metabolism and reduces blood pressure in patients with type 2 diabetes mellitus. "IndyWatch Feed Health"

PMID:  Asia Pac J Clin Nutr. 2018 ;27(2):341-346. PMID: 29384321 Abstract Title:  Astaxanthin improves glucose metabolism and reduces blood pressure in patients with type 2 diabetes mellitus. Abstract:  BACKGROUND AND OBJECTIVES: This randomized, placebo-controlled trial was performed for 8 weeks to investigate the potential effects of astaxanthin (AST) supplementation on the adiponectin concentration, lipid peroxidation, glycemic control, insulin sensitivity, and anthropometric indices in participants with type 2 diabetes mellitus.METHODS AND STUDY DESIGN: We enrolled 44 participants with type 2 diabetes who met our inclusion criteria. Eight milligrams of AST supplementation or a placebo were randomly administered once daily for 8 weeks to these participants.RESULTS: The 8-week administration of AST supplementation increased the serum adiponectin concentration and reduced visceral body fat mass (p

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04:18

Xanthohumol prevents dextran sulfate sodium-induced colitis. "IndyWatch Feed Health"

PMID:  Oncotarget. 2018 Jan 2 ;9(1):866-880. Epub 2017 Dec 12. PMID: 29416662 Abstract Title:  Xanthohumol prevents dextran sulfate sodium-induced colitis via inhibition of IKK/NF-B signaling in mice. Abstract:  Xanthohumol (XN), a prenylated chalcone isolated from the hop plant, has been reported to exhibit multiple biological functions including anti-inflammation. However, the pharmacological function of XN on colitis remains unknown. In this study, we investigated the anti-inflammatory effect of synthesized XN and molecular mechanism on dextran sulfate sodium (DSS)-induced experimental colitis. XN attenuated the colitis symptoms along with the prevention of colonic lesions after DSS challenge. XN inhibited the production of pro-inflammatory cytokines, oxidative stress and cyclooxygenase-2 expression in DSS-treated mice. Moreover, XN inhibited the phosphorylation of IB, the nuclear translocation of NF-B subunits and the transcriptional activity of NF-Band. In contrast to XN, isoXN showed much less effects on the kinase activity of IKK and IB phosphorylation induced by XN in this study, suggesting that an electrophilic carbon center present in XN is critical for the anti-inflammation in colitis, especially inhibition of IKK/NF-B signaling pathway. Consistently, our docking analysis revealed that XN could bind to the active site, presumably at the Cys99 of IKK. Taken together, these findings demonstrate a new function of XN to inhibit IKK/NF-B signaling, suggesting XN could be the potential therapeutic agent for the prevention of colitis.

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04:06

Xanthohumol may potentially be useful as a chemopreventive agent during breast hyperplasia and carcinogenesis. "IndyWatch Feed Health"

PMID:  Oncol Lett. 2018 Jan ;15(1):908-916. Epub 2017 Nov 17. PMID: 29422966 Abstract Title:  Inhibition of breast cancer cell survival by Xanthohumol via modulation of the Notch signaling pathwayand. Abstract:  Natural compounds derived from plants have been an important source of numerous clinically useful anticancer agents. Nevertheless, limited studies indicate that xanthohumol (XN), a major prenylated flavonoid in hop plants (Humulus lupulus), may possess anticarcinogenic properties. The purpose of the present study was to clarify the antitumorigenic effects and the underlying mechanism of XN on breast cancerand. A 4T1 breast tumor mouse model was used in the present study to investigate XN suppression of tumor growth as detected by tumorigenicity assays. In addition,studies revealed that XN significantly decreased cell viability, induced G/Gcell cycle arrest and apoptosis in MCF-7 and MDA-MB-231 cells, as confirmed by an MTT assay, flow cytometry and western blot analysis, indicating anticarcinogenic activity of XN against breast cancer. Furthermore, immunohistochemistry was performed to confirm the inactivation of the Notch signaling pathway, Notch 1 and Ki-67,; consistently, XN caused decreased activation of the Notch signaling pathway and apoptotic regulators B-cell lymphoma-2 (Bcl-2), Bcl-extra large and caspase 3, as determined by western blot analysis. This study suggests that XN may potentially be useful as a chemopreventive agent during breast hyperplasia and carcinogenesis, acting via the regulation of Notch associated apoptotic regulatorsand.

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03:53

These results suggest that a synergistic effect between luteolin and LED could be a new effective therapy in relieving neuroinflammation. "IndyWatch Feed Health"

PMID:  Am J Transl Res. 2018 ;10(1):283-291. Epub 2018 Jan 15. PMID: 29423013 Abstract Title:  LED enhances anti-inflammatory effect of. Abstract:  Neuroinflammation is a complex pathological process usually results from abnormal microglial activation, thus, intervention in a microglial stimulation pathway could be a promising approach for the treatment of neurodegenerative diseases. Luteolin is an important bioflavonoid possesses anti-inflammatory properties, which is widely studied over these years. Light emitting diode (LED) therapy is reported to be a potential therapeutic strategy for many diseases including neurodegenerative diseases. However, little is known about the anti-inflammatory effect of LED therapy on activated microglial cells, even less is known whether there is a synergistic anti-inflammatory effect exist in LED and luteolin therapy. In this study, we aimed to confirm the anti-inflammatory effect of luteolin and LED combination therapy in lipopolysaccharide (LPS)-stimulated BV2 microglial cells. We showed that luteolin inhibited LPS-induced cytotoxicity, tumor necrosis factor alpha (TNF) and interleukin-6 (IL-6) production through modulation of p38 and extracellular signal-regulated kinase (ERK) signaling in BV2 cells. In addition, LED therapy enhanced the anti-inflammatory effect of luteolin. These results suggest that a synergistic effect between luteolin and LED could be a new effective therapy in relieving neuroinflammation.

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03:41

Black chokeberry has protective effects against the development of Alzheimer's disease. "IndyWatch Feed Health"

PMID:  Nutr Res Pract. 2018 Feb ;12(1):13-19. Epub 2018 Jan 15. PMID: 29399292 Abstract Title:  Anti-neuroinflammatory effects of ethanolic extract of black chokeberry (L.) in lipopolysaccharide-stimulated BV2 cells and ICR mice. Abstract:  BACKGROUND/OBJECTIVES: One of the mechanisms considered to be prevalent in the development of Alzheimer's disease (AD) is hyper-stimulation of microglia. Black chokeberry (L.) is widely used to treat diabetes and atherosclerosis, and is known to exert anti-oxidant and anti-inflammatory effects; however, its neuroprotective effects have not been elucidated thus far.MATERIALS/METHODS: We undertook to assess the anti-inflammatory effect of the ethanolic extract of black chokeberry friut (BCE) in BV2 cells, and evaluate its neuroprotective effect in the lipopolysaccharide (LPS)-induced mouse model of AD.RESULTS: Following stimulation of BV2 cells by LPS, exposure to BCE significantly reduced the generation of nitric oxide as well as mRNA levels of numerous inflammatory factors such as inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), interleukin 1 beta (IL-1), and tumor necrosis factor alpha (TNF-). In addition, AD was induced in a mouse model by intraperitoneal injection of LPS (250 g/kg), subsequent to which we investigated the neuroprotective effects of BCE (50 mg/kg) on brain damage. We observed that BCE significantly reduced tissue damage inthe hippocampus by downregulating iNOS, COX-2, and TNF- levels. We further identified the quinic acids in BCE using liquid chromatography-mass spectrometry (LCMS). Furthermore, we confirmed the neuroprotective effect of BCE and quinic acid on amyloid beta-induced cell death in rat hippocampal primary neurons.CONCLUSIONS: Our findings suggest that black chokeberry has protective effects against the development of AD.

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03:32

P. vulgaris extract has a potential protective effect against UVB-induced photo aging and photo-inflammation. "IndyWatch Feed Health"

PMID:  Rejuvenation Res. 2018 Jan 29. Epub 2018 Jan 29. PMID: 29378470 Abstract Title:  Prunella vulgaris L. exerts a protective effect against extrinsic aging via NF-B, MAPKs, AP-1, and TGF-/Smad signaling pathways in UVB-aged normal human dermal fibroblasts. Abstract:  Prunella vulgaris L., a well-known traditional Chinese herbal medicine, has anti-inflammatory and antioxidant activities. In the present study, the underlying molecular mechanisms of the protective effect of P. vulgaris extract (PVE) were investigated in UVB-irradiated normal human dermal fibroblasts (NHDFs). The mRNA expression of matrix metalloproteinases (MMPs), procollagen type I and cytokines such as interleukin-6 (IL-6) and tumor necrosis factor (TNF-) was determined by RT-PCR. The expression of anti-photo aging-related signaling molecules in the NF-B, MAPK/AP-1, and TGF/Smad pathways was assessed by Western blot. We observed that PVE blocked the up-regulated production of ROS induced in UVB-irradiated NHDFs in a dose-dependent manner. Treatment with PVE also significantly ameliorated the mRNA levels of MMPs, procollagen type I, TNF-, and IL-6. In addition, the phosphorylation level of c-Jun and c-Fos was decreased through the attenuated expression levels of p-ERK and p-JNK after treatment with PVE. Furthermore, cells treated with PVE showed inhibited Smad 7 and increased Smad 2/3 expression in the TGF-/Smad signaling pathway. Hence, synthesis of procollagen type I, a precursor of collagen I, was promoted. These findings indicate that treatment with PVE has a potential protective effect against UVB-induced photo aging and photo-inflammation.

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03:16

Dried plum ingestion increases the osteoblastogenic capacity of human serum. "IndyWatch Feed Health"

PMID:  J Med Food. 2017 Jul ;20(7):653-658. Epub 2017 Apr 26. PMID: 28445075 Abstract Title:  Dried Plum Ingestion Increases the Osteoblastogenic Capacity of Human Serum. Abstract:  In cell culture studies, dried plum (Prunus domestica L.) polyphenols increased osteoblast alkaline phosphatase (ALP) activity, mineralized nodule formation, and the expression of the bone marker genes runt-related transcription factor 2 (RUNX2) and osterix. The purpose of this study was to determine whether human serum collected 1 and 2h after dried plum ingestion influenced osteoblast cell activity and gene expression. Five healthy women ingested 100g of dried plum, and serum samples were collected at baseline (before dried plum ingestion) and 1 and 2h postingestion of dried plum. MC3T3-E1 osteoblast cells were treated (2% of medium) with these serum samples for 3 or 9 days. Intracellular and extracellular ALP activities were significantly increased after 3 or 9 days of treatment with serum both postingestion time points, with no effect seen in baseline samples. Also, serum obtained 1 and 2h postingestion significantly increased the mRNA expression of bone markers RUNX2 and connexin43 (CX43) after both 3 and 9 days of incubation periods. Finally, serum obtained 1 and 2h postingestion increased the mRNA expression of -catenin after 9 days of incubation. We conclude that osteoblast activity and functionare increased by dried plum ingestion, which may, in part, explain its beneficial effects on bone health.

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03:02

A review of dried plums, prunes and bone health. "IndyWatch Feed Health"

PMID:  Nutrients. 2017 Apr 19 ;9(4). Epub 2017 Apr 19. PMID: 28422064 Abstract Title:  Dried Plums, Prunes and Bone Health: A Comprehensive Review. Abstract:  The 2015-2020 Dietary Guidelines for Americans advocate for increasing fruit intake and replacing energy-dense foods with those that are nutrient-dense. Nutrition across the lifespan is pivotal for the healthy development and maintenance of bone. The National Osteoporosis Foundation estimates that over half of Americans age 50+ have either osteoporosis or low bone mass. Dried plums, also commonly referred to as prunes, have a unique nutrient and dietary bioactive profile and are suggested to exert beneficial effects on bone. To further elucidate and summarize the potential mechanisms and effects of dried plums on bone health, a comprehensive review of the scientific literature was conducted. The PubMed database was searched through 24 January 2017 for all cell, animal, population and clinical studies that examined the effects of dried plums and/or extracts of the former on markers of bone health. Twenty-four studies were included in the review and summarized in table form. The beneficial effects of dried plums on bone health may be in part due to the variety of phenolics present in the fruit. Animal and cell studies suggest that dried plums and/or their extracts enhance bone formation and inhibit bone resorption through their actions on cell signaling pathways that influence osteoblast and osteoclast differentiation. These studies are consistent with clinical studies that show that dried plums may exert beneficial effects on bone mineral density (BMD). Long-term prospective cohort studies using fractures and BMD as primary endpoints are needed to confirm the effects of smaller clinical, animal and mechanistic studies. Clinical and prospective cohort studies in men are also needed, since they represent roughly 29% of fractures, and likewise, diverse race and ethnic groups. No adverse effects were noted among any of the studies included in this comprehensive review. While the data are not completely consistent, this review suggests that postmenopausal women may safely consume dried plums as part of their fruit intake recommendations given their potential to have protective effects on bone loss.

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02:46

Dried plum in its whole form is a promising and efficacious functional food therapy for preventing bone loss in postmenopausal women. "IndyWatch Feed Health"

PMID:  Nutrients. 2017 May 14 ;9(5). Epub 2017 May 14. PMID: 28505102 Abstract Title:  Bone-Protective Effects of Dried Plum in Postmenopausal Women: Efficacy and Possible Mechanisms. Abstract:  Osteoporosis is an age-related chronic disease characterized by a loss of bone mass and quality, and is associated with an increased risk of fragility fractures. Postmenopausal women are at the greatest risk of developing osteoporosis due to the cessation in ovarian hormone production, which causes accelerated bone loss. As the demographic shifts to a more aged population, a growing number of postmenopausal women will be afflicted with osteoporosis. Certain lifestyle factors, including nutrition and exercise, are known to reduce the risk of developing osteoporosis and therefore play an important role in bone health. In terms of nutrition, accumulating evidence suggests that dried plum (L.) is potentially an efficacious intervention for preventing and reversing bone mass and structural loss in an ovariectomized rat model of osteoporosis, as well as in osteopenic postmenopausal women. Here, we provide evidence supporting the efficacy of dried plum in preventing and reversing bone loss associated with ovarian hormone deficiency in rodent models and in humans. We end with the results of a recent follow-up study demonstrating that postmenopausal women who previously consumed 100 g dried plum per day during our one-year clinical trial conducted five years earlier retained bone mineral density to a greater extent than those receiving a comparative control. Additionally, we highlight the possible mechanisms of action by which bioactive compounds in dried plum exert bone-protective effects. Overall, the findings of our studies and others strongly suggest that dried plum in its whole form is a promising and efficacious functional food therapy for preventing bone loss in postmenopausal women, with the potential for long-lasting bone-protective effects.

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02:26

Identification of a flavonoid isolated from plum as a potent inhibitor of Hepatitis C virus entry. "IndyWatch Feed Health"

PMID:  Sci Rep. 2017 Jun 21 ;7(1):3965. Epub 2017 Jun 21. PMID: 28638096 Abstract Title:  Identification of a flavonoid isolated from plum (Prunus domestica) as a potent inhibitor of Hepatitis C virus entry. Abstract:  Hepatitis C virus (HCV) infection is a major cause of chronic liver diseases that often requires liver transplantation. The standard therapies are limited by severe side effects, resistance development, high expense and in a substantial proportion of cases, fail to clear the infection which bespeak the need for development of well-tolerated antivirals. Since most of the drug development strategies target the replication stage of viral lifecycle, the identification of entry inhibitors might be crucial especially in case of liver-transplant recipients. In the present study we have evaluated fruits which are known for their hepatoprotective effects in order to screen for entry inhibitors. We report the identification of a flavonoid, rutin, isolated from Prunus domestica as a new HCV entry inhibitor. Characterization and confirmation of the chemical structure was done by LC-ESI-MS, NMR and IR spectral analyses. Rutin significantly inhibited HCV-LP binding to hepatoma cells and inhibited cell-culture derived HCV (HCVcc) entry into hepatoma cells. Importantly, rutin was found to be non-toxic to hepatoma cells. Furthermore, rutin inhibits the early entry stage of HCV lifecycle possibly by directly acting on the viral particle. In conclusion, rutin is a promising candidate for development of anti-HCV therapeutics in the management of HCV infection.

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02:15

Water-extracted plum may have potential as a natural agent for the prevention and improvement of obesity. "IndyWatch Feed Health"

PMID:  Exp Ther Med. 2018 Feb ;15(2):1608-1615. Epub 2017 Nov 27. PMID: 29399132 Abstract Title:  Water-extracted plum () attenuates adipogenesis in murine 3T3-L1 adipocyte cells through the PI3K/Akt signaling pathway. Abstract:  The objective of the present study was to evaluate the effects of water-extracted plum (WEP) on adipocyte differentiation, adipogenesis and inflammation in differentiated 3T3-L1 adipocyte cells. WEP was assessed for basic analyses, including high-performance liquid chromatography, total phenolic and flavonoid content and antioxidant activity [1,1-diphenyl-2-picrylhydrazyl (DPPH) assays]. Moreover, the cell viability was measured using an MTT assay. Adipogenesis and lipid accumulation in 3T3-L1 adipocytes was investigated using Oil Red O staining, and the expression of genes and proteins associated with adipogenesis and lipolysis were examined by reverse transcription polymerase chain reaction and western blotting. In addition, sulforaphane using a positive control was performed simultaneously. The WEP significantly suppressed adipocyte differentiation and lipid accumulation in differentiated adipocytes without cytotoxicity. WEP resulted in direct anti-obesity effects through the modulation of adenosine monophosphate-activated protein kinase, sterol regulatory element-binding protein 1c, cytidine-cytidine-adenosine-adenosine-thymidine/enhancer binding protein and peroxisome proliferator-activated receptor . These regulations of molecular expressions were significantly activated via the phosphoinositide 3-kinase/Akt pathway. Moreover, these results provide potential anti-adipogenic effects of WEP and may have potential as a natural agent for the prevention and improvement of obesity.

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01:59

Essential oils of basil alleviated the memory impairment and hippocampal neurodegenerative changes induced by chronic unpredictable stress. "IndyWatch Feed Health"

PMID:  Metab Brain Dis. 2018 Jan 22. Epub 2018 Jan 22. PMID: 29356981 Abstract Title:  Ocimum basilicum improve chronic stress-induced neurodegenerative changes in mice hippocampus. Abstract:  Alzheimer's disease (AD), one of the progressive neurodegenerative diseases might be associated with exposure to stress and altered living conditions. This study aimed to evaluate the effectiveness of Ocimum basilicum (OB) essential oils in improving the neurodegenerative-like changes induced in mice after exposed to chronic unpredictable mild stress (CUMS). Forty male Swiss albino mice divided into four groups (n=10); the control, CUMS, CUMS+Fluoxetine, CUMS+OB were used. Behavioral tests, serum corticosterone level, hippocampus protein level of the glucocorticoid receptors (GRs) and brain-dreived neurotropic factor (BDNF) were determined after exposure to CUMS. Hippocampus was histopathologically examined. Data were analyzed using statistical package for the social sciences (SPSS) and P value of less than 0.05 was considered significant. OB diminished the depression manifestation as well as impaired short term memory observed in the mice after exposure to the CUMS as evidenced by the forced swimming and elevated plus maze test. OB also up-regulated the serum corticosterone level, hippocampal protein level of the glucocorticoid receptor and the brain-derived neurotropic factor and reduced the neurodegenerative and atrophic changes induced in the hippocampus after exposure to CUMS. Essential oils of OB alleviated the memory impairment and hippocampal neurodegenerative changes induced by exposure to the chronic unpredictable stress indicating that it is the time to test its effectiveness on patients suffering from Alzheimer disease.

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01:44

Ultra-Processed Food Linked to Cancer "IndyWatch Feed Health"

processed food cancer

By Brandon TurbevilleNatural Blaze

A new study by French researchers is drawing attention to the possible links between processed foods and cancer.

The study, which classified foods such as mass produced bread, chicken nuggets, and cakes as ultra processed, looked at 105,000 people, showed results which suggest that the more ultra-processed foods people eat, the greater their risk of cancer.

Diet, of course, is already known to affect the risk of cancer, with obesity being heavily linked to cancer rates. Processed meat has also shown an increase in rates of cancer.

Foods considered ultra processed include:

  • Mass-produced packaged breads and buns
  • Sweet or savory packaged snacks inclu...

01:27

Treatment of pancreatic cancer with intravenous vitamin C. "IndyWatch Feed Health"

PMID:  Anticancer Drugs. 2018 Feb 12. Epub 2018 Feb 12. PMID: 29438178 Abstract Title:  Treatment of pancreatic cancer with intravenous vitamin C: a case report. Abstract:  Pancreatic ductal adenocarcinoma (PDA) has a dismal prognosis and is often discovered at an advanced stage with few therapeutic options. Current conventional regimens for PDA are associated with significant morbidity, decreased quality of life, and a considerable financial burden. As a result, some patients turn to integrative medicine therapies as an alternate option after a diagnosis of PDA. Intravenous pharmacologic ascorbic acid (PAA) is one such treatment. The use of PAA has been passionately debated for many years, but more recent rigorous scientific research has shown that there are significant blood concentration differences when ascorbic acid is given parenterally when compared to oral dosing. This pharmacologic difference appears to be critical for its role in oncology. Here, we report the use of PAA in a patient with poorly differentiated stage IV PDA as an exclusive chemotherapeutic regimen. The patient survived nearly 4 years after diagnosis, with PAA as his sole treatment, and he achieved objective regression of his disease. He died from sepsis and organ failure from a bowel perforation event. This case illustrates the possibility of PAA to effectively control tumor progression and serve as an adjunct to standard of care PDA chemotherapy regimens. Our patient's experience with PAA should be taken into consideration, along with previous research in cell, animal, and clinical experiments to design future treatment trials.

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01:25

Prophylactic HPV Vaccines: Current Knowledge of Impact on Gynecological Premalignancies "IndyWatch Feed Health"

Authors: Diane M. Harper and Karen B. Williams

Abstract: Approaches for cervical cancer prevention are changing. Screening still remains the most effective method for cervical cancer prevention. Guidelines are moving to an older group of women to be screened less frequently with combinations of technologies that include biomarkers and cytology. HPV vaccination is an appropriate option for this older group of women as well, should the woman not wish to make her decision about vaccination until 21 years of age, the age of screening. Parents making decisions about HPV vaccination for their young adolescent daughters need to be fully informed that only continued screening prevents cervical cancer. HPV vaccination reduces the possibility of their daughter having an abnormal Pap test by 10% if the vaccines have not waned by the time the young adolescent becomes sexually active. HPV vaccine efficacy must last at least 15 years to contribute to the prevention of cervical cancers. At this time, protection against cervical intraepithelial neoplasia grade 2/3 (CIN 2/3) is 5 years for Gardasil and 8.4 years for Cervarix. The value of the current protection HPV vaccines offer will be viewed differently by different women. Physicians ethical duties are to provide full explanation of the risks and benefits of adding HPV vaccination to the ongoing screening programs, and to support women in their personal choice for cervical cancer prevention.

Access entire article here.

The post Prophylactic HPV Vaccines: Current Knowledge of Impact on Gynecological Premalignancies appeared first on SaneVax, Inc..

01:08

Big Pharma Stands to Lose $18.5 BILLION if Marijuana is Legalized Nationally: Is this Why the Current Administration is Attempting to Halt the Progress of Medical Cannabis? "IndyWatch Feed Health"

Marijuana medical choice dilemma health care concept as a person standing in front of two paths with one offering traditional medicine and the other option with cannabis.

by Paul Fassa
Health Impact News

A 2017 study on medical cannabis by NewFrontier Data looked at the economic impact on the pharmaceutical industry if medical cannabis were to become legal nationally:

In our latest study, From Prescription to Recommendation: How Cannabis Could Disrupt the Pharmaceutical Industry, our data analysts have found that if medical cannabis were legalized nationally, pharmaceutical expenditures on the top nine conditions commonly treated by medical cannabis could fall an estimated $18.5 billion between 2016 and 2019.

The study looks at the impact to pharmaceutical sales on the nine ailments most commonly treated with cannabis which are:

  • Chronic Pain

  • Post-Traumatic Stress Disorder (PTSD)

  • Sleep Disorders

  • Anxiety

  • Nerve Pain

  • Chemotherapy Induced Nausea and Vomiting (CINV)

  • Tourette Syndrome

  • Glaucoma...

01:03

Vitamin C and sodium bicarbonate enhance the antioxidant ability of H9C2 cells and induce heat shock proteins to relieve heat stress. "IndyWatch Feed Health"

PMID:  Cell Stress Chaperones. 2018 Feb 13. Epub 2018 Feb 13. PMID: 29442224 Abstract Title:  Vitamin C and sodium bicarbonate enhance the antioxidant ability of H9C2 cells and induce HSPs to relieve heat stress. Abstract:  Heat stress is exacerbated by global warming and affects human and animal health, leading to heart damage caused by imbalances in reactive oxygen species (ROS) and the antioxidant system, acid-base chemistry, electrolytes and respiratory alkalosis. Vitamin C scavenges excess ROS, and sodium bicarbonate maintains acid-base and electrolyte balance, and alleviates respiratory alkalosis. Herein, we explored the ability of vitamin C alone and in combination with equimolar sodium bicarbonate (Vitamin C-Na) to stimulate endogenous antioxidants and heat shock proteins (HSPs) to relieve heat stress in H9C2 cells. Control, vitamin C (20 g/ml vitamin C for 16 h) and vitamin C-Na (20 g/ml vitamin C-Na for 16 h) groups were heat-stressed for 1, 3 or 5 h. Granular and vacuolar degeneration, karyopyknosis and damage to nuclei and mitochondria were clearly reduced in treatment groups, as were apoptosis, lactate dehydrogenase activity and ROS and malondialdehyde levels, while superoxide dismutase activity was increased. Additionally, CRYAB, Hsp27, Hsp60 and Hsp70 mRNA levels were upregulated at 3 h (p

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01:00

FDA Bans Triclosan and 23 Other Antiseptics from Healthcare Products "IndyWatch Feed Health"

By Heather CallaghanEditor

Triclosan, and to a lesser extent, other antiseptics, have gone through a well-deserved pruning process with the FDA.

But its not quite over yet. In 2015, the FDA determined that anti-bacterial soaps were no more effective than regular soap. In fact, triclosan the former active ingredient in many antibacterial products required up to 9 hours to break down bacteria cell walls. However, the substance was corrosive to organs and linked to other serious health problems, putting the consumer, and especially the healthcare worker, at great personal risk.

In 2016, the FDA ordered antibacterials like triclosan out of soaps and gave companies one year to reformulate. Note: Im still seeing antibacterial soaps for sale online with this ingredient for some reason. Most companies voluntarily removed it from soap, thanks to consumer health awareness. But the chemical remained in the healthcare field. In late 2017, the FDA issued a full-out ban on the marketing of OTC healthcare products containing triclosan and other antiseptics on their hit-list.

Despite triclosan being implicated in the rise of antibiotic-resistance and considered an environmental hazard the chem...

00:47

The antineoplastic effects of sulforaphane may be reduced by the contemporaneous presence of other biological elements such as nerve growth factor. "IndyWatch Feed Health"

PMID:  Food Chem Toxicol. 2018 Jan 30. Epub 2018 Jan 30. PMID: 29407470 Abstract Title:  Anticarcinogenic activities of sulforaphane are influenced by Nerve Growth Factor in human melanoma A375 cells. Abstract:  Melanoma is a severe form of cancer, resistant to conventional therapies. According to in vitro studies, sulforaphane, a dietary component, has been considered a promising antineoplastic candidate. The present study analyzes the in vitro biological effects of sulforaphane in A375 melanoma cell line with or without the addition of Nerve Growth Factor. For the first time, our results show that a supplementation of Nerve Growth Factor partially reverses the sulforaphane-induced: i) inhibition of cell migration, ii) pro apoptotic changes in cell cycle and iii) modulation of active caspase-3. Furthermore, we report the sulforaphane-induced modulation in the expression of Nerve Growth Factor receptors TrKA and p75NTR, shifting their ratio from pro survival to pro apoptotic. In conclusion, the present study evidences that in vivo the antineoplastic effects of sulforaphane may be reduced by the contemporaneous presence of other biological elements such as Nerve Growth Factor and it contributes to a better definition of the real in vivo potentiality of sulforaphane as antineoplastic candidate.

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00:38

Sulforaphane exerts protective effects on microglia and modulates the microglial activation state. "IndyWatch Feed Health"

PMID:  Front Immunol. 2018 ;9:36. Epub 2018 Jan 23. PMID: 29410668 Abstract Title:  Sulforaphane Inhibits Lipopolysaccharide-Induced Inflammation, Cytotoxicity, Oxidative Stress, and miR-155 Expression in Murine Microglial Cells. Abstract:  Sulforaphane (SFN) is a natural product with cytoprotective, anti-inflammatory, and antioxidant effects. In this study, we evaluated the mechanisms of its effects on lipopolysaccharide (LPS)-induced cell death, inflammation, oxidative stress, and polarization in murine microglia. We found that SFN protects N9 microglial cells upon LPS-induced cell death and suppresses LPS-induced levels of secreted pro-inflammatory cytokines, tumor necrosis factor-alpha, interleukin-1 beta, and interleukin-6. SFN is also a potent inducer of redox sensitive transcription factor, nuclear factor erythroid 2-related factor 2 (Nrf2), which is responsible for the transcription of antioxidant, cytoprotective, and anti-inflammatory genes. SFN induced translocation of Nrf2 to the nucleusextracellular signal-regulated kinase 1/2 (ERK1/2) pathway activation. siRNA-mediated knockdown study showed that the effects of SFN on LPS-induced reactive oxygen species, reactive nitrogen species, and pro-inflammatory cytokine production and cell death are partly Nrf2 dependent. Mox phenotype is a novel microglial phenotype that has roles in oxidative stress responses. Our results suggested that SFN induced the Mox phenotype in murine microglia through Nrf2 pathway. SFN also alleviated LPS-induced expression of inflammatory microRNA, miR-155. Finally, SFN inhibits microglia-mediated neurotoxicity as demonstrated by conditioned medium and co-culture experiments. In conclusion, SFN exerts protective effects on microglia and modulates the microglial activation state.

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00:19

Why I spoke out against (the HPV) vaccine "IndyWatch Feed Health"

February 15, 2018: Letter to Hackney Gazette from Councillor Harvey Odze regarding HPV vaccines reprinted with authors permission.

Cllr Harvey Odze (Conservative, Springfield) writes:

I refer to your report on page 7 of the February 1 edition, where you quoted me as having said at the full council meeting on January 24:

Given the considerable body of medical evidence that HPV does not cause cervical cancer; and that the vaccine is on its way to becoming the 21st centurys Thalidomide, can you assure me all parents are given full information about the dangers of the vaccine?

I was asking Cllr Jonathan McShane, the cabinet member for health, social care and devolution, a supplementary question following on from Cllr Jessica Webbs question asking the cabinet member if we can extend the Human Papilloma Virus vaccination programme to boys.

Due to the boorish, undemocratic behavior of a group of Labour councillors, your reporter was unfortunately unable to hear the totallity of my question. So, in order to put the record straight, what I in fact asked was:

Given that there is a considerable body of peer reviewed medical evidence that Human Papilloma Virus does not cause cervical cancer and that the Human Papilloma Virus vaccine is well on its way to becoming the 21st century Thalidomide, except that Thalidomide, had it not had such disastrous side effects, would have been an effective vaccine, will the cabinet member consult the UK Association of Human Papilloma Virus Vaccine Injured Daughters (AHVID) and ensure that all parents are given full information about the dangers of the vaccine as well as its alleged advantages?

This significantly alters the balance of my question.

Since your report I have been inundated with emails of thanks and support for publicly highlighting this issue. If anyone wishes to find more information I suggest they see Joan Shentons three-part documentary series Sacrificial Virgins and the short documentary Manufactured Crisis: HPV, Hype and Horror Trailer 4.

I would suggest that any parents of injured girls who have not already done so contact AHVID, who will give them support in any way they can.

The post Why I spoke out against (the HPV) vaccine appeared first on...

00:18

Concerted redox modulation by sulforaphane alleviates diabetes and cardiometabolic syndrome. "IndyWatch Feed Health"

PMID:  Free Radic Biol Med. 2018 Feb 7. Epub 2018 Feb 7. PMID: 29427794 Abstract Title:  Concerted redox modulation by sulforaphane alleviates diabetes and cardiometabolic syndrome. Abstract:  Diabetes and cardiometabolic disorders such as hypertension and obesity are major risk factors for the development of cardiovascular disease, with a wealth of evidence suggesting that oxidative stress is linked to the initiation and pathogenesis of these disease processes. With yearly increases in the global incidence of cardiovascular diseases (CVD) and diabetes, numerous studies have focused on characterizing whether upregulating antioxidant defenses through exogenous antioxidants (e.g. vitamin E, vitamin C) or activation of endogenous defenses (e.g. the Nuclear factor erythroid 2-related factor 2 (Nrf2) antioxidant defense pathway) may be of benefit. The dietary isothiocyanate sulforaphane (SFN) is currently the subject of several clinical trials for a variety of disease states, including the evaluation of its therapeutic potential to ameliorate diabetic and cardiometabolic complications. SFN is a well characterized and potent Nrf2 inducer, however recent studies suggest its protective actions may be in part mediated by its modulation of various pro-inflammatory (e.g. Nuclear factor-kappa B (NFB)) and metabolic (e.g. Peroxisome Proliferator-Activator Receptor Gamma (PPAR)) signaling pathways. The focus of this review is to provide a detailed analysis of the known mechanisms by which SFN modulates Nrf2, NFB and PPAR signaling and crosstalk and to provide a critical evaluation of the evidence linking these transcriptional pathways with diabetic and cardiometabolic complications and SFN mediated cytoprotection. To allow comparison between rodent and human studies, we discuss the published bioavailability of SFN metabolites achieved in rodents and man in the context of Nrf2, NFB and PPAR signaling. Furthermore, we provide an update on the functional outcomes and implicated signaling pathways reported in recent clinical trials with SFN in Type 2 diabetic patients.

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00:05

Sulforaphane metabolites cause apoptosis via microtubule disruption in cancer. "IndyWatch Feed Health"

PMID:  Endocr Relat Cancer. 2018 Mar ;25(3):255-268. PMID: 29431641 Abstract Title:  Sulforaphane metabolites cause apoptosis via microtubule disruption in cancer. Abstract:  Sulforaphane (SFN) inhibited growth in many cancers, but its half-life is 2h in circulation. However, its metabolites, sulforaphane-cysteine (SFN-Cys) and sulforaphane-N-acetyl-cysteine (SFN-NAC) had longer half-lives and decreased the cell viability in both dose- and time-dependent manners in human prostate cancer. Flow cytometry assay revealed that these two SFN metabolites induced apoptosis with the features such as vacuolization, disappeared nuclear envelope, nuclear agglutination and fragmentation via transmission electron microscopy observation. Western blot showed that the sustained phosphorylation of ERK1/2 mediated by SFN metabolites caused activation andupregulation of cleaved Caspase 3 and downregulation of -tubulin. High expression of -tubulin was demonstrated to be positively correlated with cancer pathological grading. Both co-immunoprecipitation and immunofluorescence staining implicated the interaction between SFN metabolite-induced phosphorylated ERK1/2 and -tubulin, and Caspase 3 cleavage assay showed that -tubulin might be the substrate for cleaved Caspase 3. More, the SFN metabolite-mediated reduction of -tubulin increased the depolymerization and instability of microtubules by microtubule polymerization assay. Reversely,microtubule-associated protein Stathmin-1 phosphorylation was increased via phosphorylated ERK1/2 and total Stathmin-1 was reduced, which might promote over-stability of microtubules. Immunofluorescence staining also showed that SFN metabolites induced the 'nest-like' structures of microtubule distribution resulting from the disrupted and aggregated microtubules, and abnormal nuclear division, suggesting that the disturbance of spindle formation and mitosis turned up. Thus, SFN-Cys and SFN-NAC triggered the dynamic imbalance of microtubules, microtubule disruption leading to cell apoptosis. These findings provided a novel insight into the chemotherapy of human prostate cancer.

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23:44

Ursolic acid derivatives as potential antidiabetic agents. "IndyWatch Feed Health"

PMID:  Drug Dev Res. 2018 Jan 29. Epub 2018 Jan 29. PMID: 29380400 Abstract Title:  Ursolic acid derivatives as potential antidiabetic agents: In vitro, in vivo, and in silico studies. Abstract:  Hit, Lead&Candidate Discovery Protein tyrosine phosphatase 1B (PTP-1B) has attracted interest as a novel target for the treatment of type 2 diabetes, this because its role in the insulin-signaling pathway as a negative regulator. Thus, the aim of current work was to obtain seven ursolic acid derivatives as potential antidiabetic agents with PTP-1B inhibition as main mechanism of action. Furthermore, derivatives 1-7 were submitted in vitro to enzymatic PTP-1B inhibition being 3, 5, and 7 the most active compounds (IC = 5.6, 4.7, and 4.6 M, respectively). In addition, results were corroborated with in silico docking studies with PTP-1B orthosteric site A and extended binding site B, showed that 3 had polar and Van der Waals interactions in both sites with Lys120, Tyr46, Ser216, Ala217, Ile219, Asp181, Phe182, Gln262, Val49, Met258, and Gly259, showing a docking score value of -7.48 Kcal/mol, being more specific for site A. Moreover, compound 7 showed polar interaction with Gln262 and Van der Waals interactions with Ala217, Phe182, Ile219, Arg45, Tyr46, Arg47, Asp48, and Val49 with a predictive docking score of -6.43 kcal/mol, suggesting that the potential binding site could be localized in the site B adjacent to the catalytic site A. Finally, derivatives 2 and 7 (50 mg/kg) were selected to establish their in vivo antidiabetic effect using a noninsulin-dependent diabetes mice model, showing significant blood glucose lowering compared with control group (p 

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23:29

Ursolic acid atenuates high glucose-mediated mesangial cell injury. "IndyWatch Feed Health"

PMID:  Med Sci Monit. 2018 Feb 11 ;24:846-854. Epub 2018 Feb 11. PMID: 29428962 Abstract Title:  Ursolic Acid Attenuates High Glucose-Mediated Mesangial Cell Injury by Inhibiting the Phosphatidylinositol 3-Kinase/Akt/Mammalian Target of Rapamycin (PI3K/Akt/mTOR) Signaling Pathway. Abstract:  BACKGROUND To investigate the protective effect of ursolic acid (UA) on high glucose (HG)-induced human glomerular mesangial cell injury and to determine whether UA inhibits cell proliferation and reactive oxygen species (ROS) production by suppressing PI3K/Akt/mTOR pathway activation. MATERIAL AND METHODS Human mesangial cells were cultured with normal glucose (NG group), high glucose (HG group), mannitol (mannitol hypertonic control group), or high glucose with different concentrations (0.5, 1.0, and 2.0 mmol/L) of UA (HG+UA groups). Cell proliferation and intracellular ROS levels were assessed by methyl thiazolyl tetrazolium (MTT) and dichloro-dihydro-fluorescein diacetate (DCFH-DA) flow cytometry assays, respectively. Western blotting was used to detect mesangial cell expression of PI3K/Akt/mTOR pathway components, including Akt, p-Akt, mTOR, and p-mTOR, and proteins related to cell injury, including TGF-1 and fibronectin (FN). mRNA expression of TGF-1 and FN were evaluated using real-time quantitative polymerase chain reaction (PCR). RESULTS Abnormal proliferation was observed in human glomerular mesangial cells at 48 h after treatment with HG, and UA suppressed the HG-induced proliferation ofmesangial cells in a dose-dependent manner. UA inhibited ROS generation and oxidative stress in mesangial cells and mitigated mesangial cell injury. Treatment with UA reduced Akt and mTOR phosphorylation levels in mesangial cells exposed to HG (p

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22:43

Ursolic acid activates the apoptosis of prostate cancer. "IndyWatch Feed Health"

PMID:  Oncol Lett. 2018 Mar ;15(3):3202-3206. Epub 2017 Dec 27. PMID: 29435058 Abstract Title:  Ursolic acid activates the apoptosis of prostate cancer via ROCK/PTEN mediated mitochondrial translocation of cofilin-1. Abstract:  Ursolic acid has various pharmacological activities, and can reduce blood fat as well as having antihepatic, antitumoral, anti-inflammatory and antiviral properties. However, the pro-apoptotic mechanism by which ursolic acid influences human prostate cancer requires additional study. The aim of the present study was to assess whether ursolic acid activates the apoptosis of prostate cancer and to investigate the mechanism by which the Rho-associated protein kinase 1 (ROCK1)/phosphatase and tensin homolog (PTEN) signaling pathway performs a role in ursolic acid-mediated cofilin-1 to induce apoptosis in human prostate cancer. Firstly, the present study determined the pro-apoptotic mechanism by which ursolic acid influences the cell proliferation and apoptosis of human prostate LNCaP cancer cells. Caspase-3/9 activities and ROCK1, PTEN, Cofilin-1 and cytochrome c protein expression levels were also analyzed. In the present study, it is reported that the pro-apoptotic mechanism of ursolic acid potently suppressed the cell proliferation of human prostate LNCaP cancer cells. The present study revealed that the mediation of ROCK1/PTEN-cofilin-1/cytochrome c protein expression activates caspase-3/9 activities which subsequently induced the apoptosis of human prostate cancer cells. In conclusion, these findings demonstrated that ursolic acid activates the apoptosis of prostate cancer via ROCK/PTEN mediated cofilin-1/cytochrome c which mediated caspase-3/9 activities.

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22:17

Time-traveller claiming to be from 2030 PASSES a lie detector test after claiming that Donald Trump will be re-elected and Artificial Intelligence will take over "IndyWatch Feed Health"

Wouldnt it be great if we could go back in time? We could change the things from our past, stop mistakes before they happen, maybe even save the world. Unfortunately that kind of technology doesnt exist yet, but what about in the future? Well, a new video that has gone viral may prove the existence... View Article

The post Time-traveller claiming to be from 2030 PASSES a lie detector test after claiming that Donald Trump will be re-elected and Artificial Intelligence will take over appeared first on Healthy Holistic Living.

22:16

California Christian Homeschool Family Torn Apart as Children are Medically Kidnapped, Forced into Public School, and Mother is Forced out of Family Home "IndyWatch Feed Health"

Erin This is the shot from the Whidbey Island Bridge ( Point Deception) This is about an hour before he spiked the temp

This photo of the Daugherty family was taken on the Whidbey Island Bridge in Washington while the family was on vacation. Photo supplied by family.

by Health Impact News/MedicalKidnap.com Staff

When the Daugherty family decided to visit Seattle, Washington, for a 4 day whale watching trip to celebrate their sons 10th birthday, they had no idea that a trip to the emergency room would turn into a nightmare that would rip their family apart.

They were more than a thousand miles from home and from their own doctors, when chronically ill Zachary began running a fever and showing signs of an infection.

When his parents took him to the emergency room at Seattle Childrens Hospital, they blindly walked into a web of controversy involving some of the most notorious Child Abuse Specialist doctors in the country, with connections to Medical Kidnap stories we have published spanning from Boston to Arizona, California, and Washington.

California parents Kevin and Erin Daugherty learned that the conflict between Boston area doctors involved with the notorious Justina Pelletier case didnt stay in Boston. It has spread all the way from the East Coast to the West Coast of America. Their son Zachary has been caught in the crossfire of differing philosophies of different doctors.

There is a dangerous trend occurring today involving parents with children with multiple medical issues (medically complex) or children with mysterious conditions that doctors havent figured out how to diagnose yet.

While the concerned parents often have to search for doctors with the expertise to help their children, that very concern is used to accuse them of Munchausen Syndrome by Proxy or Factious Disorder, or the more common term now used in child social services Medical Child Abuse a general all-purpose term used by doctors in the relatively new field of Child Abuse Specialists when parents dare to disagree with them.

Parents versus Doctors The Fight for Ame...

22:12

Heres How to Turn Your Babys Clothes Into Keepsake Memory Bears "IndyWatch Feed Health"

The teddy bear you see below started out as a onesie. It was made out of a crafting project called memory bear, where your babys onesie can be turned into a cuddle buddy once theyve outgrown that cute outfit. Its a great keepsake for moms especially if it holds a special memory, like a hospital... View Article

The post Heres How to Turn Your Babys Clothes Into Keepsake Memory Bears appeared first on Healthy Holistic Living.

22:10

40+ Things Every Mom and Daughter Should Do Together At Least Once "IndyWatch Feed Health"

Nothing beats mother-daughter bonding moments that we can cherish forever. From simple nothings to extravagant activities, theyre always worth the fun because its in the company of someone we love. Here are 50 ideas that mothers and daughters can do to spend quality time together. Stay at Home Activities You can do a number of... View Article

The post 40+ Things Every Mom and Daughter Should Do Together At Least Once appeared first on Healthy Holistic Living.

22:08

Phenethyl isothiocyanate and ursolic acid protects DNA from oxidative damage. "IndyWatch Feed Health"

PMID:  Mol Nutr Food Res. 2018 Jan 30. Epub 2018 Jan 30. PMID: 29383876 Abstract Title:  Histone Methyltransferase Setd7 Regulates Nrf2 Signaling Pathway by Phenethyl Isothiocyanate and Ursolic Acid in Human Prostate Cancer Cells. Abstract:  SCOPE: This study aimed to investigate the role of the epigenetic regulator SET domain-containing lysine methyltransferase 7 (Setd7) in regulating the antioxidant Nrf2 pathway in prostate cancer (PCa) cells and examined the effects of two phytochemicals, phenethyl isothiocyanate (PEITC) and ursolic acid (UA).METHODS AND RESULTS: Lentivirus-mediated shRNA knockdown of Setd7 in LNCaP and PC-3 cells decreased the expression of downstream Nrf2 targets, such as NAD(P)H: quinone oxidoreductase 1 (Nqo1) and glutathione S-transferase theta 2 (Gstt2). Down-regulation of Setd7 decreased soft agar colony formation ability of PCa cells. Knockdown of Setd7 increased reactive oxygen species (ROS) generation. Furthermore, Setd7 knockdown attenuated Nqo1 and Gstt2 expression in response to HOchallenge, whereas increased DNA damage was observed in Setd7 knockdown cells in comet assay. Interestingly, Setd7 expression could be induced by the dietary phytochemicals PEITC and UA. Chromatin immunoprecipitation (ChIP) assays showed that Setd7 knockdown decreased H3K4me1 enrichment in the Nrf2 and Gstt2 promoter regions, while PEITC and UA treatments elevated the enrichment.CONCLUSION: Taken together, these results indicate that Setd7 knockdown decreases Nrf2 and Nrf2-target genes expression and that PEITC and UA induce Setd7 expression, which activates the Nrf2/antioxidant response element (ARE) signaling pathway and protects DNA from oxidative damage. This article is protected by copyright. All rights reserved.

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22:04

Dads Everywhere: We Need to Talk About Your 20-Minute Poop Breaks "IndyWatch Feed Health"

MOTHERS: KEEP READING Theres a nationwide possibly global problem frustrating moms every and it needs to be addressed. Meet Elle Beattie, an Australian award-winning radio producer and mother of 1-year-old baby boy, Lang. Now more than ever, she is your fellow mom-in-arms whos spearheading the problem and has gone viral doing so! Elle... View Article

The post Dads Everywhere: We Need to Talk About Your 20-Minute Poop Breaks appeared first on Healthy Holistic Living.

21:48

Petition Warning The World About 5G At The Winter Olympics "IndyWatch Feed Health"

By Catherine J. Frompovich

Something is going on at the 2018 Olympics in South Korea that is not part of the official sporting events, but has showed up as a prime-time radiation experiment, 5G. No one knows what to expect or how those who are exposed to the intense microwave radiation will react, since no studies have been done to ascertain long-term health effects from billions of millimeter microwave oscillation waves per second.

G stands for generation of microwaves, which are transmitted in the millimeter wave spectrum known as Gigahertz (GHz) with ranges anywhere from 30 GHz to 300 GHz.

Technically, GHz is a unit of alternating current (AC) or electromagnetic (EM) wave frequencies that equals one billion Hertz per second! What does that do to the water content and molecules in a human body?

What readers really have to know, and process mentally, is that one Gigahertz is one billion wave frequencies per second! Now, do the math; multiply that by 30 GHz and any of the ranges up to 300 GHz. I bet youre your calculator cant perform the function, as it may not have enough capacity!

However, independent research scientists say there are very serious health impacts from 5G including cataract formations and damage DNA.

...

21:40

California Christian Homeschool Family Torn Apart as Children are Medically Kidnapped, Forced into Public School, and Mother is Forced out of Family Home "IndyWatch Feed Health"

Erin This is the shot from the Whidbey Island Bridge ( Point Deception) This is about an hour before he spiked the temp

This photo of the Daugherty family was taken on the Whidbey Island Bridge in Washington while the family was on vacation. Photo supplied by family.

by Health Impact News/MedicalKidnap.com Staff

When the Daugherty family decided to visit Seattle, Washington, for a 4 day whale watching trip to celebrate their sons 10th birthday, they had no idea that a trip to the emergency room would turn into a nightmare that would rip their family apart.

They were more than a thousand miles from home and from their own doctors, when chronically ill Zachary began running a fever and showing signs of an infection.

When his parents took him to the emergency room at Seattle Childrens Hospital, they blindly walked into a web of controversy involving some of the most notorious Child Abuse Specialist doctors in the country, with connections to Medical Kidnap stories we have published spanning from Boston to Arizona, California, and Washington.

California parents Kevin and Erin Daugherty learned that the conflict between Boston area doctors involved with the notorious Justina Pelletier case didnt stay in Boston. It has spread all the way from the East Coast to the West Coast of America. Their son Zachary has been caught in the crossfire of differing philosophies of different doctors.

There is a dangerous trend occurring today involving parents with children with multiple medical issues (medically complex) or children with mysterious conditions that doctors havent figured out how to diagnose yet.

While the concerned parents often have to search for doctors with the expertise to help their children, that very concern is used to accuse them of Munchausen Syndrome by Proxy or Factious Disorder, or the more common term now used in child social services Medical Child Abuse a general all-purpose term used by doctors in the relatively new field of Child Abuse Specialists when parents dare to disagree with them.

Parents versus Doctors The Fight for Americas Chil...

21:29

A combination of phenethyl isothiocyanate and TH588 has potential as a novel therapeutic strategy against pancreatic cancer. "IndyWatch Feed Health"

PMID:  Oncol Lett. 2018 Mar ;15(3):3240-3244. Epub 2017 Dec 29. PMID: 29435064 Abstract Title:  TH588, an MTH1 inhibitor, enhances phenethyl isothiocyanate-induced growth inhibition in pancreatic cancer cells. Abstract:  Chemotherapy and radiotherapy are the most common approaches in cancer therapy. They may kill cancer cells through the generation of high levels of reactive oxygen species (ROS), which leads to oxidative DNA damage. However, tumor resistance to ROS is a problem in cancer therapy. MTH1 sanitizes oxidized dNTP pools to prevent the incorporation of damaged bases during DNA replication. Although MTH1 is non-essential in normal cells, cancer cells require MTH1 activity to avoid the incorporation of oxidized dNTPs, which would result in DNA damage and cell death. By targeting a redox-adaptation mechanism, MTH1 inhibition represents a novel therapeutic strategy against cancer. However, recent reports have indicated that growth inhibition by MTH1 inhibitors may be due to off-target cytotoxic effects. TH588, one of the first-in-class MTH1 inhibitors, kills cancer cells by an off-target effect. However, a low concentration of TH588 may effectively inhibit MTH1 activity without inhibiting cell proliferation. Phenethyl isothiocyanate (PEITC) is a dietary anticarcinogenic compound and an inducer of ROS. In the present study, it has been demonstrated that combined treatment with PEITC and TH588 effectively inhibited the growth of pancreatic cancer MIAPaCa-2 and Panc-1 cells. The antioxidant N-acetylcysteine negated this synergistic growth inhibition. PEITC and TH588 cooperatively induced the formation of 8-oxo-deoxyguanine in nuclei and pH2AX foci, a marker of DNA damage. However, the combined effects are not associated with MTH1 mRNA expression in several cancer cell lines, suggesting that the possibility of an off-target effect of TH588 cannot be eliminated. These results suggest that the combination of PEITC and TH588 has potential as a novel therapeutic strategy against pancreatic cancer.

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20:46

Inhibition of autophagy potentiates the anti-metastasis effect of phenethyl isothiocyanate in lung cancer cells. "IndyWatch Feed Health"

PMID:  Mol Carcinog. 2017 Dec 26. Epub 2017 Dec 26. PMID: 29278657 Abstract Title:  Inhibition of autophagy potentiates the anti-metastasis effect of phenethyl isothiocyanate through JAK2/STAT3 pathway in lung cancer cells. Abstract:  Phenethyl isothiocyanate (PEITC) is a natural compound abundant in cruciferous vegetables. PEITC possesses anti-tumor effect in various human malignances. Our previous study has shown that benzyl isothiocyanates (BITC) induce autophagy in lung cancer cells. However, whether autophagy play a role in the inhibitory effect of PEITC on lung cancer metastasis is unclear. In this study, we found that PEITC suppressed migration and invasion of lung cancer cells by regulating MMP2. It also induced autophagy, evidenced by the formation of acidic vesicular organelles (AVOs), the punctate pattern of LC3, the accumulation of LC3-II, and the expression of Beclin-1. Inhibition of autophagy by 3-MA and chloroquine (CQ) or knock down of Beclin-1 enhanced PEITC-caused metastasis inhibition. JAK2/STAT3 pathway was suppressed by PEITC, and further inhibited by 3-MA and CQ or Beclin-1 knock down, as a result of decreased expression of p-JAK2 and p-STAT3. Blocking JAK2/STAT3 pathway by inhibitor AG490 and Stattic suppressed cell migration and decreased the expression of MMP2, MMP9, Twist, and c-Myc. Further in vivo study showed that PEITC inhibited tumor growth, induced autophagy and suppressed JAK2/STAT3 pathway, and inhibitor CQ enhanced this effect. Taken together, our results demonstrate that PEITC inhibits metastasis potential of lung cancer cells, and induces autophagy. The autophagy induced by PEITC preserves metastasis potential of lung cancer cells, via activation of JAK2/STAT3 pathway. Inhibition of autophagy enhanced the inhibitory effect of PEITC on metastasis potential of lung cancer cells. Our finding suggests that targeting autophagy could be a promising strategy for anti-metastasis therapies.

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19:59

Shikonin inhibits cell growth and induces apoptosis in different types of breast cancer cells. "IndyWatch Feed Health"

PMID:  Sci Rep. 2018 Feb 8 ;8(1):2672. Epub 2018 Feb 8. PMID: 29422643 Abstract Title:  RNA-seq transcriptome analysis of breast cancer cell lines under shikonin treatment. Abstract:  Shikonin is a naphthoquinone isolated from the dried root of Lithospermum erythrorhizon, an herb used in Chinese medicine. Although several studies have indicated that shikonin exhibits antitumor activity in breast cancer, the mechanism of action remains unclear. In the present study, we performed transcriptome analysis using RNA-seq and explored the mechanism of action of shikonin in regulating the growth of different types of breast cancer cells. The ICof shikonin on MCF-7, SKBR-3 and MDA-MB-231 cells were 10.3, 15.0, 15.0 respectively. Our results also demonstrated that shikonin arrests the progression of cell cycle and induces apoptosis in MDA-MB-231 cells. Using RNA-seq transcriptome analysis, we found 38 common genes that significantly express in different types of breast cancer cells under shikonin treatment. In particular, our results indicated that shikonin induces the expression of dual specificity phosphatase (DUSP)-1 and DUSP2 in both RNA and protein levels. In addition, shikonin also inhibits the phosphorylation of JNK and p38, the downstream signaling molecules of DUSP1 and DUSP2. Therefore, our results suggest that shikonin induces the expression of DUSP1 and DUSP2 which consequently switches off JNK and p38 MAPK pathways and causes cell cycle arrest and apoptosis in breast cancer cells.

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19:19

Cell Phone Radiation is Unlikely to Cause Cancer: Fact or Fiction? "IndyWatch Feed Health"

According to the World Bank about three quarters of people on the planet now use a cell phone. So they must be safe, right? Wrong

Most people have some notion that cell phones communicate with one another and with cell phone towers by emitting and receiving radiation; radio frequency radiation to be more precise.

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19:15

Media is Ignoring This One Crucial Factor in Florida School Shooting "IndyWatch Feed Health"

Source: Media is Ignoring This One Crucial Factor in Florida School Shooting For more content like this visit REALfarmacy.com.

by DAVID KUPELIAN Here we go again. A horrific mass shooting occurs. Everyone is in shock and grief. Democrats blame guns and Republicans. Pundits urge the public, If you see something, say something. And everyone asks, Why? As information about the perpetrator emerges, a relative confides to a newspaper that the troubled youth who committed []

Source: Media is Ignoring This One Crucial Factor in Florida School Shooting Learn more at REALfarmacy.com - Healthy News and Information.

18:21

Melanie Gaydos: The Model with Ectodermal Dysplasia Redefining Beauty Standards "IndyWatch Feed Health"

For decades if not centuries, the modelling world catered to a specific kind of beauty. In recent years, however, 28-year-old model Melanie Gaydos is one of a growing number of alternative models collectively challenging the status quo and definition of beauty standards. How Melanie Gaydos Is Transforming the Fashion Industry It was not always photographers... View Article

The post Melanie Gaydos: The Model with Ectodermal Dysplasia Redefining Beauty Standards appeared first on Healthy Holistic Living.

18:18

The Most Important Lesson You Need To Learn This Year Based On Your Zodiac Sign "IndyWatch Feed Health"

2018 may have just started, but it already feels like its flying by. Every new year brings new challenges for us, so we better arm ourselves accordingly. Heres what you need to know for 2018, based on your zodiac sign. Lessons From Your Zodiac Sign Aries (March 21 April 19) Being an Aries, youre... View Article

The post The Most Important Lesson You Need To Learn This Year Based On Your Zodiac Sign appeared first on Healthy Holistic Living.

18:16

The Link Between Verbal Abuse and Anxiety That No One Talks About "IndyWatch Feed Health"

When you hear the term abuse, what are some of the first words or images that come to mind? Many people probably think of words like violence, beating, and hitting or images of fighting and abusive familial relationships. However, there is one type of abuse that often goes ignored verbal abuse and it... View Article

The post The Link Between Verbal Abuse and Anxiety That No One Talks About appeared first on Healthy Holistic Living.

18:11

Grandparents Never Die- They Become Invisible Everyone Must Read This Its Heartwarming! "IndyWatch Feed Health"

Growing up, our grandparents were our heroes. They were the ones we would turn to when we were upset with our parents, and they would always take your side. Theyre always there with a story to tell us, or a lesson when we need guidance. They are like a parent that you could call your... View Article

The post Grandparents Never Die- They Become Invisible Everyone Must Read This Its Heartwarming! appeared first on Healthy Holistic Living.

17:52

These Dog Foods Have Been Recalled Because They May Contain EUTHANASIA DRUGS "IndyWatch Feed Health"

By Daisy Luther

***SEE THE UPDATE FOR MORE RECALLS***

Do you feed your dogs wet food? If so, you need to check your labels because 4 major brands have been recalled by the manufacturer, J.M. Smucker Company.

According to a message sent to retailers, the foods could contain a drug that is used in euthanasia.

The J.M. Smucker Company is initiating a voluntary withdrawal of a limited quantity of Gravy Train, Kibbles N Bits, and SkippyWet Dog Food products after learning specific SKUs did not meet our specifications because a minor ingredient may contain low levels of pentobarbital. This is not a threat to pet safety. Although the extremely low levels do not present a health or safety risk, their presence in our products is not acceptable to us. (source)

In a different article, the brand Ol Roy was also included in the list of recalled brands.

Pentobarbital in pet foods can cause death.

Despite reassurances that the tainted food doesnt present a risk, a previous pentobarbital taint caused the death of one familys beloved pet. Nikki Mael shared her tragic story.

Within minutes of sharing a can of Evangers pet food among her five dogs, she was racing the lifeless animals to the emergency vet.

...

17:23

Family Members Say Florida School Shooter Was On Antidepressants for Emotional Issues "IndyWatch Feed Health"

By Rachel Blevins

Parkland, Florida At least 17 people were killed and 15 were wounded during a mass shooting at a high school in Florida on Wednesday, and the reports that have been released about the suspect indicate that he was taking medications for depression and emotional issues that come packed with dangerous side effects.

Nikolas Cruz, 19, is accused of entering Marjory Stoneman Douglas High School and opening fire on his fellow students after he was previously expelled for disciplinary reasons that have not been made public. The Miami Herald reported that Cruz has raised red flags at school in the past, and last year a teacher submitted a warning against letting him on campus with a backpack.

Former classmates said they were not surprised by the shooting. Joshua Charo told the Herald that Cruz fit the description of a disgruntled student who would carry out a mass shooting.

I cant say I was shocked, Charo said. From past experiences, he seemed like the kind of kid who would do something like this.

John Crescitelli shared the same sentiment, saying his son had warned him about Cruz before. If you were to pick one person you might predict in the future would shoot up a school or do this, it would be this kid, he said.

An Instagram account attributed to Cruz that was taken down after the shooting showed a number of photos that displayed his love for guns, knives and killing small animals and rodents.

...

16:39

Top Tips to Decrease Your Breast Cancer Risk "IndyWatch Feed Health"

Source: Top Tips to Decrease Your Breast Cancer Risk For more content like this visit REALfarmacy.com.

By Dr. Mercola Breast cancer is probably one of the most feared diagnoses a woman can get. The mere mention of it conjures up images of death, despair, or at best, disfigurement. According to breastcancer.org,1 one in eight women will develop invasive breast cancer in her lifetime, and nearly 40,000 women lose their lives to []

Source: Top Tips to Decrease Your Breast Cancer Risk Learn more at REALfarmacy.com - Healthy News and Information.

15:11

Pharma Says Opioid Vaccine On The Way "IndyWatch Feed Health"

Pharma giveth and pharma taketh; and sometimes, it does both at the exact same time. While faced with a public backlash over opioid addiction, pharmas solution is now to create a vaccine that will allegedly stop...

The post Pharma Says Opioid Vaccine On The Way appeared first on Vaxxter.

12:03

Puerto Ricos Mental Health Crisis (Podcast) "IndyWatch Feed Health"

From The New York Times: Months after Hurricane Maria devastated Puerto Rico, the island is experiencing a severe mental health crisis. Public health officials say much of the population shows signs of post-traumatic stress.

Podcast 

12:02

Drug Treatment of ADHD Tenuous Scientific Basis "IndyWatch Feed Health"

From Tidsskriftet: Over the past three decades, there has been a dramatic increase in the diagnosis and pharmacological treatment of ADHD. Recent systematic reviews reveal a weak evidence base for the use of pharmaceutical drugs in the treatment of children and adolescents diagnosed with ADHD.

Article

12:01

Psychiatrys Little Known Role in Creating the Holocaust "IndyWatch Feed Health"

From NewBostonPost: In light of the recent commemoration of International Holocaust Remembrance Day, it is important to acknowledge the role that eugenics, a field developed by psychiatrists and psychologists, played in creating the Holocaust.

The recent commemoration of International Holocaust Remembrance Day reminds us to consider the causes of the Nazi genocide. Hitler found justification for his racism in the pseudo-science of eugenics, which means good genes or good race. Eugenics was developed by psychiatrists and psychologists long before the Third Reich. According to The Master Race, an article in Boston Magazine, Massachusetts was one of its epicenters, beginning at a time when Hitler was an aimless Austrian barely in his teens. Eugenics had its roots in the beginning of psychology; it grew strong in America; and its ripest fruit was produced in Nazi Germany.

In 2010 the president of the German Association for Psychiatry and Psychotherapy, Dr. Frank Schneider, admitted and apologized for German psychiatrys leading role in the Holocaust. He said, Under National Socialism [Nazism], psychiatrists showed contempt for their fellow man. They lied to and deceived the patients in their care and their families. They forced them to be sterilized, arranged their deaths, and even performed killings. His apology made headlines in Germany but didnt get mainstream coverage in the United States or the rest of the world.

Article

11:59

I Used to Be Psychotic and Then I Heard a Voice Again "IndyWatch Feed Health"

So there I was, some 16 years or so since I last heard voices that were labelled as psychosis, and then I had another experience. I heard my name being called out from a street cafeI couldnt see anyone attached to the voice.

Having had a look around from where I was standing, I slowly took a few steps backwards towards the cafe, just to see who it was that had called my name. Alas, I could not see who it was.

What I did notice is that all the people in the cafe had appeared not to notice my name being called out. Fortunately, I was not overly concerned but perhaps a little surprised by this clear and concise calling of my name.

As I walked on, I heard the voice again. The voice was familiar, and this time I was able to put a name to the voice. When I realized who the voice was, I became unconcerned by the voice being present and continued my way.

The explanation for hearing the voice last week outside the cafe is quite straightforward and does not represent anything unusual or concerning: I had been awake for 36 hours and had spent the past 12 hours in a highly significant, humbling and deeply humane relational and emotional reality. But what if I had not been able to make sense of such an experience? What if I could not make sense of the experience of hearing the voice (especially as the voice was actually comforting given the previous few days and weeks)? And, what was the relationship between the voice I heard this week and the voices I heard all those years agoif any?

The reason this experience interested me so much is due to a number of factors, not the least of which was my personal journey of working in mental health environments that have the biomedical stamp all over them. Choosing to stand up and speak out with alternatives to the dominant discourse has come with considerable attack, allegation, agitation and threat. I have been subject to countless statements about my supposed lack of understanding, my actions increasing risk, nonsense about being anti-psychiatry, nonsense about being anti-medicationthe list goes on. This kind of vulnerability is of course not a new experience for people that have spoken out before me. But it is unfounded and rather unpleasant for me, and sadly speaks to a dominant discourse that is not founded in science, compassion, humanity or dignity, but using power as an apparent truth. The fact that I have lived experience is broadly accepted in the public mental health system where i work. The sad reality, however, is the prejudice and stereotyping that lies dormant, except for the odd occasion when people cannot contain their underlying views that a person who has previously been psychotic, diagnosed with a disorder, might just not really ever be okay. These stereotypes do inform the views of workers. More concerning is the potential consequences of the stereotypes if I returne...

11:41

Shikonin inhibits migration and invasion of thyroid cancer cells. "IndyWatch Feed Health"

PMID:  Med Sci Monit. 2018 Feb 1 ;24:661-670. Epub 2018 Feb 1. PMID: 29389913 Abstract Title:  Shikonin Inhibites Migration and Invasion of Thyroid Cancer Cells by Downregulating DNMT1. Abstract:  BACKGROUND Shikonin is a component of Chinese herbal medicine. The aim of this study was to investigate the effects of shikonin on cell migration of papillary thyroid cancer cells of the TPC-1 cell line in vitro and expression levels of the phosphate and tensin homolog deleted on chromosome 10 (PTEN) and DNA methyltransferase 1 (DNMT1) genes. MATERIAL AND METHODS The Cell Counting Kit-8 (CCK-8) assay was performed to evaluate the proliferation of TPC-1 papillary thyroid cancer cells, and the normal thyroid cells, HTori-3, in vitro. A transwell motility assay was used to analyze the migration of TPC-1 cells. Western blot was performed to determine the expression levels of PTEN and DNMT1 genes. A methylation-specific polymerase chain reaction (PCR) (MSP) assay was used to evaluate the methylation of PTEN. RESULTS Following treatment with shikonin, the cell survival rate of TPC-1 cells decreased in a dose-dependent manner; the inhibitory effects on HTori-3 cells were less marked. Shikonin inhibited TPC-1 cell migration and invasion in a dose-dependent manner. The methylation of PTEN was suppressed by shikonin, which also reduced the expression of DNMT1 in a dose-dependent manner, and increased the expression of PTEN. Overexpression of DNMT1 promoted the migration of TPC-1 cells and the methylation of PTEN. Levels of protein expression of PTEN in TPC-1 cells treated with shikonin decreased, and were increased by DNMT1 knockdown. CONCLUSIONS Shikonin suppressed the expression of DNMT1, reduced PTEN gene methylation, and increased PTEN protein expression, leading to the inhibition of TPC-1 cell migration.

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11:19

These results provide in-depth insight into the mechanisms of action of shikonin in the treatment of cancer. "IndyWatch Feed Health"

PMID:  Exp Ther Med. 2018 Feb ;15(2):1484-1492. Epub 2017 Dec 5. PMID: 29434733 Abstract Title:  Shikonin causes apoptosis by disrupting intracellular calcium homeostasis and mitochondrial function in human hepatoma cells. Abstract:  Shikonin is known to suppress proliferation and induce apoptosis in a variety of cancer cell lines. In the present study, SMMC-7721 human hepatocellular carcinoma cells were treated with shikonin (1, 2 or 4M) for 12-48 h. Cell morphological alterations and DNA damage were determined. Furthermore, changes in cell cycle, mitochondrial transmembrane potential, calcium homeostasis and levels of reactive oxygen species were measured. Shikonin-treated SMMC-7721 cells exhibited morphological changes and DNA damage. Shikonin inhibited cell proliferation causing cell cycle arrest at the G/Gphase and induced apoptosis in a dose- and time-dependent manner. Shikonin-induced apoptosis was associated with activation of caspases-3, -8 and -9, elevated levels of intracellular Caand reactive oxygen species, reduced mitochondrial membrane potential and enhanced efflux of Caand K. Gene expression B-cell lymphoma 2 (Bcl-2)-associated X protein (Bax), p53 and caspase-3 was up-regulated, whereas Bcl-2 expression was downregulated. Shikonin caused apoptosis by inhibiting cell cycle progression, disrupting Cahomeostasis, inducing oxidative stress and triggering mitochondrial dysfunction. Activation of caspases-3, -8 and -9, Kefflux, and regulation of Bax, Bcl-2, p53 and caspase-3 expression are involved in the process. These results provide in-depth insight into the mechanisms of action of shikonin in the treatment of cancer.

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08:17

The use of wavelength-exclusion filters in biophotonic measurement can be employed to detect cancer in vitro. "IndyWatch Feed Health"

PMID:  Biochem Biophys Rep. 2018 Mar ;13:7-11. Epub 2017 Nov 20. PMID: 29202105 Abstract Title:  Biophotonic markers of malignancy: Discriminating cancers using wavelength-specific biophotons. Abstract:  Early detection is a critically important factor when successfully diagnosing and treating cancer. Whereas contemporary molecular techniques are capable of identifying biomarkers associated with cancer, surgical interventions are required to biopsy tissue. The common imaging alternative, positron-emission tomography (PET), involves the use of nuclear material which poses some risks. Novel, non-invasive techniques to assess the degree to which tissues express malignant properties are now needed. Recent developments in biophoton research have made it possible to discriminate cancerous cells from normal cells both in vitro and in vivo. The current study expands upon a growing body of literature where we classified and characterized malignant and non-malignant cell types according to their biophotonic activity. Using wavelength-exclusion filters, we demonstrate that ratios between infrared and ultraviolet photon emissions differentiate cancer and non-cancer cell types. Further, we identified photon sources associated with three filters (420-nm, 620-nm., and 950-nm) which classified cancer and non-cancer cell types. The temporal increases in biophoton emission within these wavelength bandwidths is shown to be coupled with intrisitic biomolecular events using Cosic's resonant recognition model. Together, the findings suggest that the use of wavelength-exclusion filters in biophotonic measurement can be employed to detect cancer in vitro.

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08:00

Practicing super brain yoga reduced the symptoms of hyperactivity disorder among the school-age children. "IndyWatch Feed Health"

PMID:  Perspect Psychiatr Care. 2018 Feb 10. Epub 2018 Feb 10. PMID: 29427513 Abstract Title:  Effectiveness of super brain yoga for children with hyperactivity disorder. Abstract:  PURPOSE: This study aimed at determining the effectiveness of super brain yoga for children with hyperactivity disorder.DESIGN AND METHOD: This quasiexperimental and interventional research was conducted on 80 school-age children through a pretest-posttest design.RESULTS: Results showed that there was a significant difference between the means of severity of hyperactivity disorder before (75.1) and after the intervention (63.5) (p 

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07:54

A review of yoga and immune system functioning. "IndyWatch Feed Health"

PMID:  J Behav Med. 2018 Feb 10. Epub 2018 Feb 10. PMID: 29429046 Abstract Title:  Yoga and immune system functioning: a systematic review of randomized controlled trials. Abstract:  Yoga is an ancient mind-body practice that is increasingly recognized to have health benefits in a variety of clinical and non-clinical conditions. This systematic review summarizes the findings of randomized controlled trials examining the effects of yoga on immune system functioning which is imperative to justify its application in the clinic. Fifteen RCTs were eligible for the review. Even though the existing evidence is not entirely consistent, a general pattern emerged suggesting that yoga can downregulate pro-inflammatory markers. In particular, the qualitative evaluation of RCTs revealed decreases in IL-1beta, as well as indications for reductions in IL-6 and TNF-alpha. These results imply that yoga may be implemented as a complementary intervention for populations at risk or already suffering from diseases with an inflammatory component. Beyond this, yoga practice may exert further beneficial effects by enhancing cell-mediated and mucosal immunity. It is hypothesized that longer time spans of yoga practice are required to achieve consistent effects especially on circulating inflammatory markers. Overall, this field of investigation is still young, hence the current body of evidence is small and for most immune parameters, more research is required to draw distinct conclusions.

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07:42

Regular stretching exercise of Thai yoga in combination with exercises could promote health-related physical fitness. "IndyWatch Feed Health"

PMID:  J Altern Complement Med. 2018 Feb 13. Epub 2018 Feb 13. PMID: 29437484 Abstract Title:  The Effects of Thai Yoga on Physical Fitness: A Meta-Analysis of Randomized Control Trials. Abstract:  OBJECTIVES: Thai yoga is a traditional Thai exercise used for improving health-related physical fitness. Many studies have evaluated these effects, but their results remain inconclusive. This meta-analysis aimed to examine the effectiveness of Thai yoga on physical fitness.DESIGN/METHODS: PubMed, EMBASE, CINAHL, Cochrane library, Thai Library Integrated System (ThaiLis), Physiotherapy Evidence Database (PEDro), National Rehabilitation Information Center (Rehabdata), Scopus, Web of Science, Thai University library databases/journals, and Thai Physical Therapy database up to March 2016 were searched for randomized controlled trials (RCTs) examining the effect of Thai yoga exercise compared with normal daily activities as controls, in any language. The weighted mean difference (WMD) and 95% confidence intervals (95% CI) were performed using the random-effects model.RESULTS: Seven RCTs met the inclusion criteria. Thai yoga training significantly improved body flexibility by 3.9cm after 4 weeks [95% CI=3.9-4.0; p

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07:25

Allicin alleviated lipid metabolism disorder induced by 1,3-DCP. "IndyWatch Feed Health"

PMID:  Biomed Pharmacother. 2017 Dec ;96:1411-1417. Epub 2017 Nov 21. PMID: 29169723 Abstract Title:  Protective effects of allicin on 1,3-DCP-induced lipid metabolism disorder in HepG2 cells. Abstract:  Allicin (2-propene-1-sulfinothioic acid S-2-propenyl ester), with quite a good range of hepatoprotective and antineoplastic properties, is a functional substance from garlic (Allium sativum L.) The purpose of this study was to provide evidence that allicin could protect 1,3-DCP-induced lipid metabolism disorder in HepG2 cells. Allicin reduced the accumulation of triglycerides (TG) and total cholesterol (TC) in 1,3-DCP-induced HepG2 cells. Allicin significantly increased the phosphorylation of AMP-activated protein kinase (AMPK) and down-regulated the levels of sterol regulatory element binding protein-1 (SREBP-1) and sterol regulatory element binding protein-2 (SREBP-2) in 1,3-DCP-induced HepG2 cells. Additionally, allicin had obvious recovery influence on the phosphorylation level of PKA and CREB in 1,3-DCP-induced HepG2 cells. These observations indicated that allicin alleviated lipid metabolism disorder induced by 1,3-DCP in HepG2 cells by regulating AMPK-SREBPs and PKA-CREB signaling pathways.

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07:09

Allicin ameliorates cognitive impairment in APP/PS1 mice. "IndyWatch Feed Health"

PMID:  Tissue Cell. 2018 Feb ;50:89-95. Epub 2017 Dec 2. PMID: 29429523 Abstract Title:  Allicin ameliorates cognitive impairment in APP/PS1 mice via Suppressing oxidative stress by Blocking JNK Signaling Pathways. Abstract:  In middle and old age, Alzheimer's disease (AD) is a progressive neurodegenerative disorder of brain. As an increasingly aging population, AD represents a huge burden for the patients' family and the country. However, current therapeutical strategies have shown limited effectiveness. Allicin, which is the main composition of garlic, was reported to prevent the learning and memory impairment of AD mouse model. As the mechanism is not clear, in this study, we used the APP (amyloid precursor protein)/PS1 (presenilin 1) double transgenic mice, which express human mutant APP and PS1, to determine the protective effect of allicin on neurons. AD involves a broad range of clinical, cellular, and biochemical manifestations. This has led to many views of AD, e.g. the amyloid, presenilin, oxidative stress, and mitochondrial dysfunction. We confirmed that allicin improves the cognitive function of APP/PS1 double transgenic mice by reducing the expression levels of A, oxidative stress, and improving mitochondrial dysfunction. Application of behavioral, morphological and molecular biology, and other means were conducted to investigate the effect of allicin, which provide an experimental basis for the reliable application of allicin in the treatment of neurodegenerative diseases.

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07:00

All Homeopathic Products Now Illegal? "IndyWatch Feed Health"

Source: All Homeopathic Products Now Illegal? For more content like this visit REALfarmacy.com.

By Dr. Mercola About 5 million U.S. adults and 1 million U.S. children use homeopathy every year, and the remedies are known to be generally safe and unlikely to cause severe adverse reactions, according to the U.S. National Institutes of Healths National Center for Complementary and Integrative Health.1 Yet, if the U.S. Food and Drug []

Source: All Homeopathic Products Now Illegal? Learn more at REALfarmacy.com - Healthy News and Information.

07:00

How to Grow Sage "IndyWatch Feed Health"

By Dr. Mercola

Known for its woolly oblong leaves and musky smell, sage (Salvia officinalis) is one of the easiest herbs to grow. While its ornamental varieties are also popular, sage is best known in its culinary form as a complement to poultry and other meat dishes. In the U.S., a mention of sage is likely to evoke memories of Thanksgiving turkey and stuffing, two dishes in which sage often features prominently.

Beyond its culinary uses, sage has medicinal value to aid digestion, boost cognitive function, manage diabetes and treat inflammation, among others. Fortunately, it is one of the easiest perennial herbs to grow. If you are able to provide plenty of sunshine, good drainage and air circulation, you should consider growing sage.

Options for Starting Sage

Starting sage plants couldnt be easier, and you have three options:1,2

Seed: Sage seed must be sown while fresh. Not only does it not store well, but also, even when the seed is fresh, sage is a relatively slow and poor germinator. When planting indoors, place three seeds in a cell to ensure you get a decent amount of viable seedlings.

Root cuttings: You can grow another sage plant by clipping a 3-inch portion from the very tip of an existing mature stem and placing it in moist potting mix or vermiculite. In about six weeks, roots will emerge. Another option is layering,3 which involves securing a bendable stem from an existing plant to the ground using a piece of wire or rocks.

Before covering it with dirt, make a small nick in the bent stem where you want the roots to grow. You can wait a couple of months or a whole season before separating the new plant from the original.

Transplants:4 If you want to get a head start on the growing season, plant sage seeds in a starter soil mix and maintain them under grow lights for six to eight weeks before the last frost in your area. The seeds will take about three weeks to germinate. Another option is to purchase established transplants from your local garden center or nursery.

...

Start Your Parsley Plants Indoors "IndyWatch Feed Health"

By Dr. Mercola

Whether you prefer curly or flat varieties, parsley is more than a garnish or natural breath freshener. Rich in vitamins K, A and C, as well as minerals like calcium and iron, parsley can be easily grown outdoors when ground temperatures are above 50 degrees F, or indoors year-round. Despite being a slow germinator, parsley does well when maintained in moist, well-drained soil with decent sun exposure.

Parsley is packed with flavonoids and volatile oils known to provide numerous health benefits. This nutrient-rich herb has been shown to act as a diuretic, aid diabetes management, build strong bones, fight cancer, reduce inflammation and strengthen your immune system. Parsley can be incorporated into fresh vegetable juice, blended into smoothies, used to season a variety of foods and transformed into a cleansing tea. Whatever your pleasure, give parsley a try.

Parsley: An Ancient Herb

Parsley (Petroselinum crispum) has a long, rich history, dating back 2,000 years.1,2 It originated in the central Mediterranean region. Later, it became naturalized to other areas of Europe. Today, parsley is grown widely and available worldwide as a fresh herb or dried spice. As a member of the Apiaceae (or Umbelliferae) family, parsley makes its home among other aromatic herbs such as anise, coriander, cumin, dill and fennel, as well as vegetables like carrots, celery and parsnips.

Parsley was highly prized by ancient Greeks, who incorporated it into crowns and wreaths. They also used parsley in burial rituals and hung it on tombs. The Romans initially used parsley as a medicinal plant and later consumed it as a food. The use of parsley as a seasoning is believed to have caught on during the Middle Ages. The curly variety was more popular initially because the flat-leaf type could easily be confused for fools parsley, a poisonous weed.

Today, parsley is used as an herb, green leafy vegetable and spice. Parsley leaf and root are not only popular in European and Mediterranean cuisines, but also have found their way into a variety of international cuisines. Beyond being an attractive garnish, parsley adorns salads, enlivens stocks and...

06:52

Allicin induces apoptosis through activation of both intrinsic and extrinsic pathways in glioma cells. "IndyWatch Feed Health"

PMID:  Mol Med Rep. 2018 Feb 2. Epub 2018 Feb 2. PMID: 29436625 Abstract Title:  Allicin induces apoptosis through activation of both intrinsic and extrinsic pathways in glioma cells. Abstract:  Allicin is an extract purified from Allium sativum (garlic), and previous research has indicated that Allicin has an inhibitory effect on many kinds of tumor cells. The aim of the present study was to explore the anticancer activity of Allicin on human glioma cells and investigate the underlying mechanism. MTT and colony-formation assays were performed to detect glioma cell proliferation, and explore the effect of Allicin at various doses and time-points. The apoptosis of glioma cells was measured by fluorescence microscopy with Hoechst 33258 staining, and then flow cytometry was used to analyzed changes in glioma cell apoptosis. Reverse transcriptionquantitative polymerase chain reaction and western blot analysis were used to detect the effect of Allicin on the expression levels of Fas/Fas ligand (FasL), caspase3, Bcell lymphoma 2 and Bcl2associated X protein. Allicin suppressed the proliferation and colony formation ability of U251 cells in a dose and timedependent manner. A cytotoxic effect of Allicin was observed in glioma cells in a dosedependent manner. Changes in nuclear morphology were observed in U251 cells with Hoechst 33258 staining. The activity of caspases were significantly elevated and Fas/FasL expression levels were increased following treatment with Allicin, at both the mRNA and protein level. These results demonstrated that Allicin suppresses proliferation and induces glioma cell apoptosis in vitro. Both intrinsic mitochondrial and extrinsic Fas/FasLmediated pathways react in glioma cell after treating with Allicin, which then activate major apoptotic cascades. These results implicate Allicin as a novel antitumor agent in treating glioma.

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06:38

Polyphenols have potential use in the prevention and treatment of cardiovascular diseases. "IndyWatch Feed Health"

PMID:  Curr Pharm Des. 2018 Jan 29. Epub 2018 Jan 29. PMID: 29384050 Abstract Title:  Polyphenols: Potential Use In The Prevention And Treatment Of Cardiovascular Diseases. Abstract:  BACKGROUND: Polyphenols are bioactive compounds that can be found mostly in foods like fruits, cereals, vegetables, dry legumes, chocolate and beverages such as coffee, tea and wine. They are extensively used in the prevention and treatment of cardiovascular disease (CVD) providing protection against many chronic illnesses. Their effects on human health depend on the amount consumed and on their bioavailability. Many studies have demonstrated that polyphenols have also good effects on the vascular system by lowering blood pressure, improving endothelial function, increasing antioxidant defences, inhibiting platelet aggregation and low-density lipoprotein oxidation, and reducing inflammatory responses.METHODS: This review is focused on some groups of polyphenols and their effects on several cardiovascular risk factors such as hypertension, oxidative stress, atherogenesis, endothelial dysfunction, carotid artery intima-media thickness, diabetes and lipid disorders.RESULTS: It is proved that these compounds have many cardio protective functions: they alter hepatic cholesterol absorption, triglyceride biosynthesis and lipoprotein secretion, the processing of lipoproteins in plasma, and inflammation. In some cases, human long-term studies did not show conclusive results because they lacked in appropriate controls and in an undefined polyphenol dosing regimen.CONCLUSION: Rigorous evidence is necessary to demonstrate whether or not polyphenols beneficially impact CVD prevention and treatment.

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06:07

Studies on prevention of obesity, metabolic syndrome, diabetes, cardiovascular diseases and cancer by tea. "IndyWatch Feed Health"

n/a PMID:  J Food Drug Anal. 2018 Jan ;26(1):1-13. Epub 2017 Dec 1. PMID: 29389543 Abstract Title:  Studies on prevention of obesity, metabolic syndrome, diabetes, cardiovascular diseases and cancer by tea. Abstract:  Tea, a popular beverage made from leaves of the plant Camellia sinensis, has been studied extensively in recent decades for its beneficial health effects in the prevention of obesity, metabolic syndrome, diabetes, cancer, and other diseases. Whereas these beneficial effects have been convincingly demonstrated in most laboratory studies, results from human studies have not been consistent. Some studies demonstrated that weight reduction, alleviation of metabolic syndrome and risk reduction in diabetes were only observed in individuals who consume 3-4 cups of tea (600-900 mg tea catechins) or more daily. This chapter reviews some of these studies, the possible mechanisms of actions of tea constituents, and the challenges in extrapolating laboratory studies to human situations.

05:49

730,000 Filipino Children Receive Faulty Dengue Vaccine Which Causes Dengue Rather Than Preventing it "IndyWatch Feed Health"

Source: 730,000 Filipino Children Receive Faulty Dengue Vaccine Which Causes Dengue Rather Than Preventing it For more content like this visit REALfarmacy.com.

In a story that is being mostly censored in the U.S. media, vaccine manufacturer Sanofi Pasteur has admitted that their vaccine for dengue (a deadly tropical disease spread mostly by mosquitoes) is defective. In a press release from France, the pharmaceutical company admitted that the vaccine is harmful to those not previously infected with dengue, []

Source: 730,000 Filipino Children Receive Faulty Dengue Vaccine Which Causes Dengue Rather Than Preventing it Learn more at REALfarmacy.com - Healthy News and Information.

05:38

may be effectively used in controlling or treating obesity, hyperlipidemia and other related metabolic diseases. "IndyWatch Feed Health"

PMID:  J Food Sci. 2018 Feb 10. Epub 2018 Feb 10. PMID: 29427445 Abstract Title:  Green Tea Polyphenols Modulate Colonic Microbiota Diversity and Lipid Metabolism in High-Fat Diet Treated HFA Mice. Abstract:  : There is an increasing interest in the effect of dietary polyphenols on the intestinal microbiota and the possible associations between this effect and the development of obesity. However, limited information is available on how these polyphenols affect the gut microbiota and lipid metabolism. The co-action of a high-fat diet (HFD) and tea polyphenol (TP) on gut microbiota and lipid metabolism using a human flora-associated (HFA) C57BL/6J mice model is studied. TP reduced serum total cholesterol, triglyceride, low density lipoprotein, glucose (GLU) and insulin (INS) levels of HFD mice in a dose-dependent manner (P

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05:26

The results show the effects and mechanism of Oolong tea to provide cardio-protective benefits during hypoxic conditions. "IndyWatch Feed Health"

PMID:  Environ Toxicol. 2018 Feb ;33(2):220-233. Epub 2017 Nov 15. PMID: 29139225 Abstract Title:  Oolong tea prevents cardiomyocyte loss against hypoxia by attenuating p-JNK mediated hypertrophy and enhancing P-IGF1R, p-akt, and p-Badactivity and by fortifying NRF2 antioxidation system. Abstract:  Tea, the most widely consumed natural beverage has been associated with reduced mortality risk from cardiovascular disease. Oolong tea is a partially fermented tea containing high levels of catechins, their degree of oxidation varies between 20%-80% causing differences in their active metabolites. In this study we examined the effect of oolong tea extract (OTE) obtained by oxidation at low-temperature for short-time against hypoxic injury and found that oolong tea provides cyto-protective effects by suppressing the JNK mediated hypertrophic effects and by enhancing the innate antioxidant mechanisms in neonatal cardiomyocytes and in H9c2 cells. OTE effectively attenuates 24 h hypoxia-triggered cardiomyocyte loss by suppressing caspase-3-cleavage and apoptosis in a dose-dependent manner. OTE also enhances the IGFIR/p-Akt associated survival-mechanism involving the elevation of p-Badin a dose-dependent manner to aid cellular adaptations against hypoxic challenge. The results show the effects and mechanism of Oolong tea to provide cardio-protective benefits during hypoxic conditions.

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05:18

Fenghuang Oolong teas may have prebiotic-like activity and can be used as functional food components in manipulating intestinal microbiota. "IndyWatch Feed Health"

PMID:  Int J Food Sci Nutr. 2018 Jan 5:1-15. Epub 2018 Jan 5. PMID: 29303032 Abstract Title:  The evaluation of the quality of Feng Huang Oolong teas and their modulatory effect on intestinal microbiota of high-fat diet-induced obesity mice model. Abstract:  The variations in the contents of tea catechins and free amino acids in relation to the quality of Fenghuang Oolong teas (FOT) were determined. It demonstrated that in FOT, which were grown at a high altitude, the contents of methylated estered tea catechins were relatively higher. By human flora-associated (HFA) mice model, the effect of FOT on high-fat diet-induced obesity was investigated by high-throughput sequencing. The shifts in relative abundance of the dominant taxa at the phylum, family and genus levels showed their dramatically effects. A large increase in Bacteroidetes with decrease of Firmicutes was observed after the administration of FOT for 8weeks. Together, these results suggest that FOT are rich in tea catechins, especially O-methylated tea catechin derivatives, which may be affected by the unique growth environment, and FOT may have prebiotic-like activity and can be used as functional food components in manipulating intestinal microbiota.

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05:06

Tea infusions ameliorate cancer in 4TI metastatic breast cancer model. "IndyWatch Feed Health"

PMID:  BMC Complement Altern Med. 2017 Apr 7 ;17(1):202. Epub 2017 Apr 7. PMID: 28388918 Abstract Title:  Tea (Camellia sinensis) infusions ameliorate cancer in 4TI metastatic breast cancer model. Abstract:  BACKGROUND: Tea (Camellia sinensis) infusions are widely consumed beverages with numerous health benefits. However, physiological and molecular responses mediating these activities are poorly understood.METHOD: Three replicates of 4TI cancer cell suspension (2.0  10cells/ml) were challenged in vitro with various concentrations of green, black and purple tea infusions to asseses their cytoxicity and associated differentially expressed genes in the cells. Inhibitory activity was tested by using serial dilutions of respective tea infusions in a 96 well ELISA plate.RESULTS: Green tea had the highest inhibition on 4TI cells proliferation at a concentration of IC = 13.12 g/ml. Further analysis of the 4TI cancer cell line treated with tea using 454 pyrosequencing generated 425,696 reads with an input mean length of 286.54. Trimmed sequences were imported on a CLC genomic workbench v7.03 and annotated on a reference mouse genome (Mus musculus strain C57BL/6 J). Results revealed a differential expression of apoptosis related genes in the transcriptome. Casp8, Casp9, Casp3, Casp6, Casp8AP2, Aifm1, Aifm2 and Apopt1 genes were significantly upregulated indicating the process of apoptosis was initiated and executed.CONCLUSION: These findings on caspases offer valuable information on the mechanism of tea as an anticancer agent and will contribute to further research in future novel treatments.

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04:55

Keemun black tea extract contains potent fatty acid synthase inhibitors and reduces food intake and body weight of rats. "IndyWatch Feed Health"

PMID:  J Enzyme Inhib Med Chem. 2005 Aug ;20(4):349-56. PMID: 16206829 Abstract Title:  Keemun black tea extract contains potent fatty acid synthase inhibitors and reduces food intake and body weight of rats via oral administration. Abstract:  The inhibitory effects of a black tea extract on fatty acid synthase were measured through inhibition kinetics. The Keemun black tea extract showed more potent inhibitory activity on fatty acid synthase than green tea extract. Additionally, the inhibitory ability of the black tea extract depended on the extracting solvent and the conditions used. Only 10-23% of the inhibitory activity from the black tea was extracted by the general method of boiling with water. The results suggested that the main fatty acid synthase inhibitors in black tea might be theaflavins. Which were more potent than epigallocatechin gallate or C75. The reaction site on the fatty acid synthase and the inhibition kinetics for the extract were different from those of epigallocatechin gallate or C75. In addition, Keemun black tea extract significantly reduced food intake, body weight and blood triglyceride of diet-induced obesity SD rats via oral administration.

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04:43

Polysaccharides extracted from Keemun black tea has an observable prevention of liver damage induced by carbon tetrachloride in mice. "IndyWatch Feed Health"

PMID:  Food Chem Toxicol. 2013 Aug ;58:184-92. Epub 2013 Apr 24. PMID: 23623843 Abstract Title:  Protective effects of Keemun black tea polysaccharides on acute carbon tetrachloride-caused oxidative hepatotoxicity in mice. Abstract:  This study was designed to investigate chemical characterization of the water-soluble polysaccharides extracted from Keemun black tea (KBTP), and their antioxidant and hepatoprotective effects against CCl4-induced oxidative damage in mice. HPLC analysis revealed that KBTP is the typical acidic heteropolysaccharides and consisted of nine monosaccharides. Furthermore, KBTP showed highly ferric-reducing antioxidant power and scavenging effects against DPPH, OH and O2(-) in vitro. Administration of KBTP (200, 400 and 800 mg/kg bw) in mice ahead of CCl4 injection could observably antagonize the CCl4-induced increases in serum ALT, AST, TG and TC, and the hepatic MDA and 8-iso-PGF2a levels, respectively. Mice with KBTP pretreatment displayed a better profile of hepatosomatic index and the improved GSH and SOD activities in comparison with CCl4-intoxicated mice. These biochemical results were further supported with liver histopathological assessment, revealing that KBTP has an observable prevention of liver damage induced by CCl4 in mice.

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04:29

The inhibitory effects of Keemun black tea on -amylase and -glucosidase were positively correlated to tea grades. "IndyWatch Feed Health"

PMID:  Food Chem. 2018 Apr 25 ;246:74-81. Epub 2017 Nov 8. PMID: 29291881 Abstract Title:  An emerging strategy for evaluating the grades of Keemun black tea by combinatory liquid chromatography-Orbitrap mass spectrometry-based untargeted metabolomics and inhibition effects on-glucosidase and -amylase. Abstract:  Quantitative analysis and untargeted liquid chromatography mass spectrum (LC-MS) based metabolomics of different grades of Keemun black tea (KBT) were conducted. Quantitative analysis did not show tight correlation between tea grades and contents of polyphenols, but untargeted metabolomics analysis revealed that high-grades KBT were distinguished from the low-grades. S-plot and Variable Importance (VIP) analysis gave 28 marker compounds responsible for the discrimination of different grades of KBT. The inhibitory effects of KBT on-amylase and -glucosidase were positively correlated to tea grades, and the correlation coefficient between each marker compound and inhibitory rate were calculated. Thirteen compounds were positively related to the anti-glycemic activity, and theasinensin A, afzelechin gallate and kaempferol-glucoside were confirmed as grade-related bioactive marker compounds by chemical and bioassay in effective fractions. This study suggested that combinatory metabolomics and bioactivities assay provided a new strategy for the classification of tea grades.

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04:23

28days of polyphenol supplementation modulates the acute JNK, BAD, and Bcl2 response to heavy resistance exercise. "IndyWatch Feed Health"

PMID:  Physiol Rep. 2018 Jan ;6(2). PMID: 29380956 Abstract Title:  Polyphenol supplementation alters intramuscular apoptotic signaling following acute resistance exercise. Abstract:  The purpose of this study was to examine the effects of 28-days of supplementation with an aqueous proprietary polyphenol blend (PPB) sourced from Camellia sinensis on intramuscular apoptotic signaling following an acute lower-body resistance exercise protocol and subsequent recovery. Untrained males (n = 38, 21.8  2.7 years, 173.4  7.9 cm, 77.6  14.6 kg) were randomized to PPB (n = 14), placebo (PL; n = 14) or control (CON; n = 10). Participants completed a lower-body resistance exercise protocol comprised of the squat, leg press, and leg extension exercises. Skeletal muscle microbiopsies were obtained from the vastus lateralis preexercise (PRE), 1-h (1HR), 5-h (5HR), and 48-h (48HR) post-resistance exercise. Apoptotic signaling pathways were quantified using multiplex signaling assay kits to quantify total proteins (Caspase 3, 8, 9) and markers of phosphorylation status (JNK, FADD, p53, BAD, Bcl-2). Changes in markers of muscle damage and intramuscular signaling were analyzed via separate repeated measures analysis of variance (ANOVA). Change in Bcl-2 phosphorylation at 1H was significantly greater in PL compared to CON (P = 0.001). BAD phosphorylation was significantly elevated at 5H in PL compared to PPB (P = 0.015) and CON (P = 0.006). The change in JNK phosphorylation was significantly greater in PPB (P = 0.009), and PL (P = 0.017) compared to CON at 1H, while the change for PL was elevated compared to CON at 5H (P = 0.002). A main effectwas observed (P 

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04:11

Polyphenols from green tea, oolong tea and black tea may modulate the intestinal flora and generate short chain fatty acids. "IndyWatch Feed Health"

PMID:  J Food Sci Technol. 2018 Jan ;55(1):399-407. Epub 2017 Dec 2. PMID: 29358833 Abstract Title:  The modulatory effect of polyphenols from green tea, oolong tea and black tea on human intestinal microbiota in vitro. Abstract:  In the present study, polyphenols from green tea (GTP), oolong tea (OTP) and black tea (BTP) were prepared by extraction with hot water and polyamide column chromatography. In antioxidant assay in vitro, each tea polyphenols exhibited potential activity; the intestinal absorption of GTP, OTP and BTP was investigated individually by Caco-2 transwell system, and each sample was poorly transported, illustrating a low transport rate for tea polyphenols through cell monolayers. The effects of GTP, OTP and BTP on human intestinal microbiota were also evaluated, and each sample induced the proliferation of certain beneficial bacteria and inhibited-and. Moreover, the short-chain fatty acids (SCFA) produced in cultures with tea polyphenols were relatively higher. Together, these results suggested GTP, OTP and BTP may modulate the intestinal flora and generate SCFA, and contribute to the improvements of human health.

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03:52

Comparative studies on the hypolipidemic, antioxidant and hepatoprotective activities of catechin-enriched green and oolong tea "IndyWatch Feed Health"

PMID:  Food Funct. 2018 Jan 31. Epub 2018 Jan 31. PMID: 29384173 Abstract Title:  Comparative studies on the hypolipidemic, antioxidant and hepatoprotective activities of catechin-enriched green and oolong tea in a double-blind clinical trial. Abstract:  This study aimed to compare the beneficial effect of catechin-enriched green tea and oolong tea on mildly hypercholesterolemic subjects. Sixty mildly hypercholesterolemic subjects (180-220 mg dL) were enrolled and divided into three groups as catechin-enriched green tea (CEGT), catechin-enriched oolong tea (CEOT) or placebo. The subjects were instructed to drink 2 300 mL of CEGT (780.6 mg of catechin), CEOT (640.4 mg of catechin) or placebo beverage for 12 weeks. Drinking CEGT and CEOT significantly decreased (p

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03:49

Video: Fake News, Treason, Sedition, and the Deep State "IndyWatch Feed Health"

Sarah Corriher explores how the media and the deep state are working in tandem to undermine America's Republic, and to cause President Trump to lose his mainstream support through a campaign of violence and sabotage of the financial markets.

You may watch this on YouTube if there are any problems watching the video at this site. It can also be viewed at Spkout.

03:35

Aged oolong tea reduces high-fat diet-induced fat accumulation and dyslipidemia. "IndyWatch Feed Health"

PMID:  Nutrients. 2018 Feb 8 ;10(2). Epub 2018 Feb 8. PMID: 29419789 Abstract Title:  Aged Oolong Tea Reduces High-Fat Diet-Induced Fat Accumulation and Dyslipidemia by Regulating the AMPK/ACC Signaling Pathway. Abstract:  While oolong tea (OT) has been shown to induce weight loss and reduce fat accumulation, the mechanisms remain poorly defined, especially for aged OT. In this study, five groups of mice (= 9/group) were used including a normal diet with vehicle treatment, and a high-fat diet (HFD) with vehicle or the water extracts from aged OTs (EAOTs, three different storage years) by oral gavage at 1000 mg/kgBW for 6 weeks. Body weight, fat accumulation, and serum biochemical parameters were used to evaluate obesity. The morphology of hepatocytes and adipocytes was analyzed by being stained with hematoxylin and eosin. The levels of p-AMPK, p-ACC (and non-phosphorylated versions), CPT-1 and FAS were determined by Western blotting and immunohistochemistry. EAOTs decreased HFD-induced body weight, fat accumulation, serum levels of triglyceride, total cholesterol, and low-density lipoprotein cholesterol, while enhancing the serum high-density lipoprotein cholesterol level. At the same time, EAOTs clearly alleviated fatty liver and reduced the size of adipocytes in the epididymal fat, especially in the 2006 group. Most importantly, EAOTs increased the phosphorylation of AMPK and ACC, and up-regulated the expression of CPT-1 but down-regulated the expression of fatty acid synthase, TNF- and iNOS. Thus, EAOTs may inhibit obesity by up-regulating energy expenditure and fatty acid oxidation while inhibiting fatty acid synthesis and inflammation.

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03:33

Oral health expert Robin Whyman slams claims made by fluoride critic Paul Connett "IndyWatch Feed Health"

DHB clinical director for oral health Dr Robin Whyman says evidence shows fluoride can help reduce dental decay. Photo / File

DHB clinical director for oral health Dr Robin Whyman says evidence shows fluoride can help reduce dental decay. Photo / File

Fluoridating drinking water is about protecting the health interests of the wider population, a dental expert says.

This comes in response to a Fluoride Free meeting held on Wednesday, where prominent critic Dr Paul Connett told a 100-person audience about water fluoridation and the harm he argued it posed to health and the environment.

However, Hawkes Bay District Health Board clinical director for oral health Dr Robin Whyman said the board supported fluoridation as evidence showed its effect on reducing dental decay, particularly in children.

We think its good for our community, and we think it has a positive health benefit which will reduce peoples need for treatment and reduce cost both to the DHB and to peoples pockets.

Their position was consistent with that of the Ministry of Health, and other DHBs around the country. It was based on research which had been repeatedly backed up in both national and international studies, he said.

The Wednesday meeting was part of a New Zealand speaking tour featuring Dr Connett, a retired professor of environmental chemistry and toxicology at St Lawrence University, as the guest of Fluoride Free NZ.

His presentation touched on a number of ar...

03:08

Fermented oolong tea high in gallic acid as an effective dietary intervention strategy for tobacco-associated cancers. "IndyWatch Feed Health"

PMID:  Oncotarget. 2018 Jan 2 ;9(1):361-374. Epub 2017 Dec 7. PMID: 29416619 Abstract Title:  The inhibitory activity of gallic acid against DNA methylation: application of gallic acid on epigenetic therapy of human cancers. Abstract:  Epigenome aberrations have been observed in tobacco-associated human malignancies. (-)-epigallocatechin-3-gallate (EGCG) has been proven to modulate gene expression by targeting DNA methyltransferases (DNMTs) through a proposed mechanism involving the gallate moiety of EGCG. We show that gallic acid (GA) changes the methylome of lung cancer and pre-malignant oral cell lines and markedly reduces both nuclear and cytoplasmic DNMT1 and DNMT3B within 1 week. GA exhibits stronger cytotoxicity against the lung cancer cell line H1299 than EGCG. We found that GA reactivates the growth arrest and DNA damage-inducible 45 (GADD45) signaling pathway may through the demethylation of CCNE2 and CCNB1 in H1299 cells. To improve the epigenetic anti-cancer activities of oolong tea, we identified a fungus,which can efficiently increase the GA content in oolong tea via a 2-week fermentation process. The fungus dramatically increased GA up to 44.8 fold in the post-fermentation oolong tea extract (PFOTE), resulting in enhanced demethylation effects and a significant reduction in the nuclear abundances of DNMT1, DNMT3A, and DNMT3B in lung cancer cell lines. PFOTE also showed stronger anti-proliferation activities than oolong tea extract (OTE) and increased sensitivity to cisplatin in H1299 cells. In summary, we demonstrate the potent inhibitory effects of GA on the activities of DNMTs and provide a strong scientific foundation for the use of specialized fermented oolong tea high in GA as an effective dietary intervention strategy for tobacco-associated cancers.

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02:34

Florida School Shooting - and the State of American Education "IndyWatch Feed Health"

This is one of the times I wish with all my heart Dan Olmsted was alive to help guide this conversation. On Wednesday, a 19 year old kid shot and killed 17 students and faculty at a Florida high school....

02:23

Interested In Muscle? Eat Whole Eggs Rather Than Egg Whites "IndyWatch Feed Health"

Egg yolks also contain over 80 percent of the overall vitamins and minerals that can be found within the egg as a whole. The yolk also contains enzymes which help the body absorb the protein in the white. People who consume protein from whole eggs or from egg whites after engaging in exercise differ dramatically in how their muscles build protein, a process called protein synthesis, during the post-workout period, researchers report in a new study.

02:16

EGCG may potentially inhibit aging-induced cardiac hypertrophy, fibrosis, and apoptosis, thereby preserving cardiac function. "IndyWatch Feed Health"

PMID:  J Cardiovasc Pharmacol. 2018 Feb ;71(2):65-75. PMID: 29419571 Abstract Title:  Ameliorative Effect of Epigallocatechin Gallate on Cardiac Hypertrophy and Fibrosis in Aged Rats. Abstract:  The objective of the present study is to evaluate the effect of epigallocatechin gallate (EGCG) on aging-mediated cardiac hypertrophy, fibrosis, and apoptosis. The Wistar albino rats were divided into 4 groups (n = 18). Group I: young (3 months), group II: aged (24-26 months), group III: aged + EGCG (200 mg/kg for 30 days), and group IV: young + EGCG. At the end of 30 days, EGCG administration to the aged animals showed significant (P

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02:03

EGCG could potentially serve as an additional therapeutic intervention in the arsenal against obesity. "IndyWatch Feed Health"

PMID:  Food Funct. 2018 Feb 9. Epub 2018 Feb 9. PMID: 29423494 Abstract Title:  EGCG evokes Nrf2 nuclear translocation and dampens PTP1B expression to ameliorate metabolic misalignment under insulin resistance condition. Abstract:  As a major nutraceutical component of green tea (-)-epigallocatechin-3-gallate (EGCG) has attracted interest from scientists due to its well-documented antioxidant and antiobesity bioactivities. In the current study, we aimed to investigate the protective effect of EGCG on metabolic misalignment and in balancing the redox status in mice liver and HepG2 cells under insulin resistance condition. Our results indicated that EGCG accelerates the glucose uptake and evokes IRS-1/Akt/GLUT2 signaling pathway via dampening the expression of protein tyrosine phosphatase 1B (PTP1B). Consistently, ectopic expression of PTP1B by Ad-PTP1B substantially impaired EGCG-elicited IRS-1/Akt/GLUT2 signaling pathway. Moreover, EGCG co-treatment stimulated nuclear translocation of Nrf2 by provoking P13K/AKT signaling pathway and thus modulated the downstream expressions of antioxidant enzymes such as HO-1 and NQO-1 in HepG2 cells. Furthermore, knockdown Nrf2 by small interfering RNA (siRNA) notably enhanced the expression of PTP1B and blunt EGCG-stimulated glucose uptake. Consistent with these results, in vivo study revealed that EGCG supplement significantly ameliorated high-fat and high-fructose diet (HFFD)-triggered insulin resistance and oxidative stress by up-regulating the IRS-1/AKT and Keap1/Nrf2 transcriptional pathways. Administration of an appropriate chemopreventive agent, such as EGCG, could potentially serve as an additional therapeutic intervention in the arsenal against obesity.

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01:50

EGCG could be a novel treatment of neuro-inflammation and neurodegenerative diseases. "IndyWatch Feed Health"

PMID:  Mol Neurobiol. 2018 Feb 9. Epub 2018 Feb 9. PMID: 29427085 Abstract Title:  Regulatory Effects of Neuroinflammatory Responses Through Brain-Derived Neurotrophic Factor Signaling in Microglial Cells. Abstract:  Inhibition of microglial over-activation is an important strategy to counter balance neurodegenerative progression. We previously demonstrated that the adenosine monophosphate-activated protein kinase (AMPK) may be a therapeutic target in mediating anti-neuroinflammatory responses in microglia. Brain-derived neurotrophic factor (BDNF) is one of the major neurotrophic factors produced by astrocytes to maintain the development and survival of neurons in the brain, and have recently been shown to modulate homeostasis of neuroinflammation. Therefore, the present study focused on BDNF-mediated neuroinflammatory responses and may provide an endogenous regulation of neuroinflammation. Among the tested neuroinflammation, epigallocatechin gallate (EGCG) and minocycline exerted BDNF upregulation to inhibit COX-2 and proinflammatory mediator expressions. Furthermore, both EGCG and minocycline upregulated BDNF expression in microglia through AMPK signaling. In addition, minocycline and EGCG also increased expressions of erythropoietin (EPO) and sonic hedgehog (Shh). In the endogenous modulation of neuroinflammation, astrocyte-conditioned medium (AgCM) also decreased the expression of COX-2 and upregulated BDNF expression in microglia. The anti-inflammatory effects of BDNF were mediated through EPO/Shh in microglia. Our results indicated that the BDNF-EPO-Shh novel-signaling pathway underlies the regulation of inflammatory responses and may be regarded as a potential therapeutic target in neurodegenerative diseases. This study also reveals a better understanding of an endogenous crosstalk between astrocytes and microglia to regulate anti-inflammatory actions, which could provide a novel strategy for the treatment of neuroinflammation and neurodegenerative diseases.

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01:35

EGCG inhibited self-renewal and expression of pluripotency-maintaining transcription factors in human cancer stem cells. "IndyWatch Feed Health"

PMID:  Mol Cells. 2018 Jan 31. Epub 2018 Jan 31. PMID: 29429153 Abstract Title:  Cancer Prevention with Green Tea and Its Principal Constituent, EGCG: from Early Investigations to Current Focus on Human Cancer Stem Cells. Abstract:  Cancer preventive activities of green tea and its main constituent, (-)-epigallocatechin gallate (EGCG) have been extensively studied by scientists all over the world. Since 1983, we have studied the cancer chemopreventive effects of EGCG as well as green tea extract and underlying molecular mechanisms. The first part of this review summarizes groundbreaking topics with EGCG and green tea extract: 1) Delayed cancer onset as revealed by a 10-year prospective cohort study, 2) Prevention of colorectal adenoma recurrence by a double-blind randomized clinical phase II trial, 3) Inhibition of metastasis of B16 melanoma cells to the lungs of mice, 4) Increase in the average value of Young's moduli, i.e., cell stiffness, for human lung cancer cell lines and inhibition of cell motility and 5) Synergistic enhancement of anticancer activity against human cancer cell lines with the combination of EGCG and anticancer compounds. In the second part, we became interested in cancer stem cells (CSCs). 1) Cancer stem cells in mouse skin carcinogenesis by way of introduction, after which we discuss two subjects from our review on human CSCs reported by other investigators gathered from a search of PubMed, 2) Expression of stemness markers of human CSCs compared with their parental cells, and 3) EGCG decreases or increases the expression of mRNA and protein in human CSCs. On this point, EGCG inhibited self-renewal and expression of pluripotency-maintaining transcription factors in human CSCs. Human CSCs are thus a target for cancer prevention and treatment with EGCG and green tea catechins.

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01:21

Inhibitory effect of the green tea molecule EGCG against dengue virus infection. "IndyWatch Feed Health"

PMID:  Arch Virol. 2018 Feb 10. Epub 2018 Feb 10. PMID: 29429035 Abstract Title:  Inhibitory effect of the green tea molecule EGCG against dengue virus infection. Abstract:  Dengue virus (DENV) infection is a major public health problem worldwide; however, specific antiviral drugs against it are not available. Hence, identifying effective antiviral agents for the prevention of DENV infection is important. In this study, we showed that the reportedly highly biologically active green-tea component epigallocatechin gallate (EGCG) inhibited dengue virus infection regardless of infecting serotype, but no or minimal inhibition was observed with other flaviviruses, including Japanese encephalitis virus, yellow fever virus, and Zika virus. EGCG exerted its antiviral effect mainly at the early stage of infection, probably by interacting directly with virions to prevent virus infection. Our results suggest that EGCG specifically targets DENV and might be used as a lead structure to develop an antiviral drug for use against the virus.

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01:07

EGCG exerts a neuroprotective effect on experimental autoimmune thyroiditis. "IndyWatch Feed Health"

PMID:  Exp Ther Med. 2018 Jan ;15(1):1087-1092. Epub 2017 Nov 14. PMID: 29434699 Abstract Title:  Neuroprotective effect of (-)-epigallocatechin-3-gallate on autoimmune thyroiditis in a rat model by an anti-inflammation effect, anti-apoptosis and inhibition of TRAIL signaling pathway. Abstract:  (-)-Epigallocatechin-3-gallate (EGCG) is a polyphenol monomer compound extracted and separated from green tea, and is a key catechin in green tea. Recent research has identified that EGCG is equipped with important biological activities, including antitumor, antioxidant, anti-inflammation, blood fat reduction and radiation protection abilities. In the current study, it was investigated whether EGCG exerts a neuroprotective effect on AIT and examined the possible underlying mechanism. The present study sought to establish an experimental autoimmune thyroiditis (AIT) rat model and to investigate the neuroprotective effect of EGCG in this model. EGCG was demonstrated to inhibit urinary iodine values and thyroid pathological features in AIT model rats. Treatment with EGCG significantly reduced interleukin-1, interferon- (INF-) and tumor necrosis factor- (TNF-) levels in the AIT rats through suppression of nuclear factor-B (NF-B) pathway. In addition, pretreatment with EGCG significantly increased B-cell lymphoma-2 protein expression, and suppressed caspase-3 activity and TNF--related apoptosis-inducing ligand (TRAIL) protein expression levels in the AIT model rats. In conclusion, these results suggested that the neuroprotective effect of EGCG protects against AIT through its anti-inflammatory ability, anti-apoptosis and TRAIL signaling pathway in model rats, and it may be used asa therapeutic agent against AIT caused by inflammation.

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00:30

Hempseed peptides exert hypocholesterolemic effects with a statin-like mechanism. "IndyWatch Feed Health"

PMID:  J Agric Food Chem. 2017 Oct 11 ;65(40):8829-8838. Epub 2017 Oct 2. PMID: 28931275 Abstract Title:  Hempseed Peptides Exert Hypocholesterolemic Effects with a Statin-Like Mechanism. Abstract:  This study had the objective of preparing a hempseed protein hydrolysate and investigating its hypocholesterolemic properties. The hydrolysate was prepared treating a total protein extract with pepsin. Nano HPLC-ESI-MS/MS analysis permitted identifying in total 90 peptides belonging to 33 proteins. In the range 0.1-1.0 mg/mL, it inhibited the catalytic activity of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCoAR) in a dose-dependent manner. HepG2 cells were treated with 0.25, 0.5, and 1.0 mg/mL of the hydrolysate. Immunoblotting detection showed increments in the protein levels of regulatory element binding proteins 2 (SREBP2), low-density lipoprotein receptor (LDLR), and HMGCoAR. However, the parallel activation of the phospho-5'-adenosine monophosphate-activated protein kinase (AMPK) pathway, produced an inactivation of HMGCoAR by phosphorylation. The functional ability of HepG2 cells to uptake extracellular LDL was raised by 50.5 2.7%, 221.5 1.6%, and 109 3.5%, respectively, versus the control at 0.25, 0.5, and 1.0 mg/mL concentrations. Finally, also a raise of the protein level of proprotein convertase subtilisin/kexintype 9 was observed. All of these data suggest that the mechanism of action has some similarity with that of statins.

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00:23

The anti-inflammatory activity of Cannabis extracts on colon epithelial cells derives from a fraction of the extract that contains THCA. "IndyWatch Feed Health"

PMID:  Cannabis Cannabinoid Res. 2017 ;2(1):167-182. Epub 2017 Jul 1. PMID: 29082314 Abstract Title:  Anti-Inflammatory Activity in Colon Models Is Derived from9-Tetrahydrocannabinolic Acid That Interacts with Additional Compounds inExtracts. Abstract:  Inflammatory bowel diseases (IBDs) include Crohn's disease, and ulcerative colitis.preparations have beneficial effects for IBD patients. However,extracts contain hundreds of compounds. Although there is much knowledge of the activity of different cannabinoids and their receptor agonists or antagonists, the cytotoxic and anti-inflammatory activity of wholeextracts has never been characterized in detail withandcolon models.The anti-inflammatory activity ofextracts was studied on three lines of epithelial cells and on colon tissue.flowers were extracted with ethanol, enzyme-linked immunosorbent assay was used to determine the level of interleukin-8 in colon cells and tissue biopsies, chemical analysis was performed using high-performance liquid chromatography, mass spectrometry and nuclear magnetic resonance and gene expression was determined by quantitative real-time PCR.The anti-inflammatory activity of Cannabis extracts derives from D9-tetrahydrocannabinolic acid (THCA) present in fraction 7 (F7) of the extract. However, all fractions ofat a certain combination of concentrations have a significant increased cytotoxic activity. GPR55 receptor antagonist significantly reduces the anti-inflammatory activity of F7, whereas cannabinoid type 2 receptor antagonist significantly increases HCT116 cell proliferation. Also, cannabidiol (CBD) shows dose dependent cytotoxic activity, whereas anti-inflammatory activity was found only for the low concentration of CBD, and in a bell-shaped rather than dose-dependent manner. Activity of the extract and active fraction was verified on colon tissues taken from IBD patients, and was shown to suppress cyclooxygenase-2 () and metalloproteinase-9 () gene expression in both cell culture and colon tissue.It is suggested that the anti-inflammatory activity of Cannabis extracts on colon epithelial cells derives from a fraction of the extract that contains THCA, and is mediated, at least partially, via GPR55 receptor. The cytotoxic activity of theextract was increased by combining all fractions at a certain combination of concentrations and was partially affected by CB2 receptor antagonist that increased cell proliferation. It is suggested that in a nonpsychoactive treatment for IBD, THCA should be used rather than CBD.

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00:00

An extract of fructus cannabis ameliorates learning and memory impairment induced by d-galactose. "IndyWatch Feed Health"

PMID:  Evid Based Complement Alternat Med. 2017 ;2017:4757520. Epub 2017 Oct 19. PMID: 29234402 Abstract Title:  Extract of Fructus Cannabis Ameliorates Learning and Memory Impairment Induced by D-Galactose in an Aging Rats Model. Abstract:  Hempseed (L.) has been used as a health food and folk medicine in China for centuries. In the present study, we sought to define the underlying mechanism by which the extract of Fructus Cannabis (EFC) protects against memory impairment induced by D-galactose in rats. To accelerate aging and induce memory impairment in rats, D-galactose (400mg/kg) was injected intraperitoneally once daily for 14 weeks. EFC (200 and 400mg/kg) was simultaneously administered intragastrically once daily in an attempt to slow the aging process. We found that EFC significantly increased the activity of superoxide dismutase, while lowering levels of malondialdehyde in the hippocampus. Moreover, EFC dramatically elevated the organ indices of some organs, including the heart, the liver, the thymus, and the spleen. In addition, EFC improved the behavioral performance of rats treated with D-galactose in the Morris water maze. Furthermore, EFC inhibited the activation of astrocytes and remarkably attenuated phosphorylated tau and suppressed the expression of presenilin 1 in the brain of D-galactose-treated rats. These findings suggested that EFC exhibits beneficial effects on the cognition of aging rats probably by enhancing antioxidant capacityand anti-neuroinflammation, improving immune function, and modulating tau phosphorylation and presenilin expression.

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Thursday, 15 February

23:31

C. sativa decreased tau and stathmin gene expression and cancer metastasis. "IndyWatch Feed Health"

PMID:  Iran J Basic Med Sci. 2017 Oct ;20(10):1178-1181. PMID: 29147495 Abstract Title:  Standardizedextract attenuates tau and stathmin gene expression in the melanoma cell line. Abstract:  Objectives: Metastasis is the main cause of death in patients with melanoma. Cannabis-based medicines are effective adjunctive drugs in cancer patients. Tau and Stathmin proteins are the key proteins in cancer metastasis. Here we have investigated the effect of a standardizedextract on cell migration and Tau and Stathmin gene expression in the melanoma cell line.Materials and Methods: In the treatment group, melanoma (B1617) was treated 48 hr with various concentrations of standardizedextract. Cells with no treatment were considered as the control group, then study was followed by Quantitative RT-Real Time PCR assay. Relative gene expression was calculated by thect method. Migration assay was used to evaluate cancer metastasis.Results: Tau and stathmin gene expression was significantly decreased compared to the control group. Cell migration was also significantly reduced compared to controls.Conclusion: decreased tau and stathmin gene expression and cancer metastasis. The results may have some clinical relevance for the use of cannabis-based medicines in patients with metastatic melanoma.

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23:17

Extracts of Cannabis with high content of non-psychotropic cannabinoids significantly inhibit aldose reductase activity. "IndyWatch Feed Health"

PMID:  Fitoterapia. 2018 Feb 7. Epub 2018 Feb 7. PMID: 29427593 Abstract Title:  Inhibition of aldose reductase activity by Cannabis sativa chemotypes extracts with high content of cannabidiol or cannabigerol. Abstract:  Aldose reductase (ALR2) is a key enzyme involved in diabetic complications and the search for new aldose reductase inhibitors (ARIs) is currently very important. The synthetic ARIs are often associated with deleterious side effects and medicinal and edible plants, containing compounds with aldose reductase inhibitory activity, could be useful for prevention and therapy of diabetic complications. Non-psychotropic phytocannabinoids exert multiple pharmacological effects with therapeutic potential in many diseases such as inflammation, cancer, diabetes. Here, we have investigated the inhibitory effects of extracts and their fractions from two Cannabis sativa L. chemotypes with high content of cannabidiol (CBD)/cannabidiolic acid (CBDA) and cannabigerol (CBG)/cannabigerolic acid (CBGA), respectively, on human recombinant and pig kidney aldose reductase activity in vitro. A molecular docking study was performed to evaluate the interaction of these cannabinoids with the active site of ALR2 compared to known ARIs. The extracts showed significant dose-dependent aldose reductase inhibitory activity (>70%) and higher than fractions. The inhibitory activity of the fractions was greater for acidic cannabinoid-rich fractions. Comparative molecular docking results have shown a higher stability of the ALR2-cannabinoid acids complex than the other inhibitors. The extracts of Cannabis with high content of non-psychotropic cannabinoids CBD/CBDA or CBG/CBGA significantly inhibit aldose reductase activity. These results may have some relevance for the possible use of C. sativa chemotypes based preparations as aldose reductase inhibitors.

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22:49

These findings support efficacy of artisanal CBD preparations in seizure reduction with few significant side effects. "IndyWatch Feed Health"

PMID:  Epilepsy Behav. 2018 Feb 8. Epub 2018 Feb 8. PMID: 29429908 Abstract Title:  Efficacy of artisanal preparations of cannabidiol for the treatment of epilepsy: Practical experiences in a tertiary medical center. Abstract:  Medically refractory epilepsy continues to be a challenge worldwide, and despite an increasing number of medical therapies, approximately 1 in 3 patients continues to have seizures. Cannabidiol (CBD), one of many constituents of the Cannabis sativa or marijuana plant, has received renewed interest in the treatment of epilepsy. While highly purified CBD awaits Food and Drug Administration (FDA) approval, artisanal formulations of CBD are readily available and are seeing increased use in our patient population. Although randomized controlled trials of CBD are ongoing and promising, data regarding artisanal formulations of CBD are minimal and largely anecdotal. Here, we report a retrospective study to define the efficacy of artisanal CBD preparations in children with epilepsy. Given the known interaction between CBD and clobazam, we also conducted a subgroup comparison to determine if clobazam use was related to any beneficial effects of CBD. Additionally, we compared response rates with CBD and with clobazam alone within an overlapping patient cohort. A pediatric cohort with epilepsy of 108 patients was identified through a medical record search for patients using CBD oil. The addition of CBD resulted in 39% of patients having a>50% reduction in seizures, with 10% becoming seizure-free. The responder rate for clobazam was similar. No patients achieved CBD monotherapy, although the weaning of other antiepileptic drugs (AEDs) became possible in 22% of patients. A comparable proportion had AED additions during CBD therapy. With concomitant use of clobazam, 44% of patients had a 50% reduction in seizures upon addition of CBD compared with 33% in the population not taking clobazam; this difference was not statistically significant. The most common reported side effect of CBD was sedation in less than 4% of patients, all of whom were also taking clobazam. Increased alertness and improved verbal interactions were reported in 14% of patients in the CBD group and 8% of patients in the CBD and clobazam group. Benefits were more marked in the CBD alone group, in contrast to the CBD and clobazam group, but this difference was not statistically significant. In summary, these findings support efficacy of artisanal CBD preparations in seizure reduction with few significant side effects. The response to CBD was independent of concurrent clobazam use, although clobazam may contribute to the sedation seen with concurrent CBD use.

...

22:26

This data further supports the evaluation of CBD and CBD-rich extracts for the potential treatment of endometrial cancer. "IndyWatch Feed Health"

PMID:  J Physiol Biochem. 2018 Feb 13. Epub 2018 Feb 13. PMID: 29441458 Abstract Title:  Cannabinoid-induced cell death in endometrial cancer cells: involvement of TRPV1 receptors in apoptosis. Abstract:  Among a variety of phytocannabinoids,-tetrahydrocannabinol (THC) and cannabidiol (CBD) are the most promising therapeutic compounds. Besides the well-known palliative effects in cancer patients, cannabinoids have been shown to inhibit in vitro growth of tumor cells. Likewise, the major endocannabinoids (eCBs), anandamide (AEA) and 2-arachidonoylglycerol (2-AG), induce tumor cell death. The purpose of the present study was to characterize cannabinoid elements and evaluate the effect of cannabinoids in endometrial cancer cell viability. The presence of cannabinoid receptors, transient receptor potential vanilloid 1 (TRPV1), and endocannabinoid-metabolizing enzymes were determined by qRT-PCR and Western blot. We also examined the effects and the underlying mechanisms induced by eCBs and phytocannabinoids in endometrial cancer cell viability. Besides TRPV1, both EC cell lines express all the constituents of the endocannabinoid system. We observed that at concentrations higher than 5 M, eCBs and CBD induced a significant reduction in cell viability in both Ishikawa and Hec50co cells, whereas THC did not cause any effect. In Ishikawa cells, contrary to Hec50co, treatment with AEA and CBD resulted in an increase in the levels of activated caspase -3/-7, in cleaved PARP, and inreactive oxygen species generation, confirming that the reduction in cell viability observed in the MTT assay was caused by the activation of the apoptotic pathway. Finally, these effects were dependent on TRPV1 activation and intracellular calcium levels. These data indicate that cannabinoids modulate endometrial cancer cell death. Selective targeting of TPRV1 by AEA, CBD, or other stable analogues may be an attractive research area for the treatment of estrogen-dependent endometrial carcinoma. Our data further support the evaluation of CBD and CBD-rich extracts for the potential treatment of endometrial cancer, particularly, that has become non-responsive to common therapies.

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21:14

NHRC issues notices to Cgarh Govt, Ministry of Drinking Water over high levels of fluoride "IndyWatch Feed Health"

New Delhi, Feb 15 The National Human Rights Commission (NHRC) on Thursday issued notices to the Chief Secretary, Government of Chhattisgarh and the Secretary, Union Ministry of Drinking Water and Sanitation, calling for the detailed reports over the increasing number of patients due to high levels of fluoride and arsenic in drinking water, within four weeks.

The Commission has taken suo motu cognizance of a media report that despite the joint efforts of the Centre and the Government of Chhattisgarh, the number of patients suffering from fluorosis and kidney diseases is increasing due to high level of arsenic and fluoride in drinking water in the state, an official statement said.

The number of total 1148 patients till August, 2016, has gone up now to 1170. The villages adjacent to the industrial areas have been reported to be worst affected. These include, among others, areas in Bastar, Raigarh, Bemetara districts.

According to the data released by the government, 55 people have died in Supebeda of Gariaband district. However, villagers claim that 103 deaths have happened due to the diseases caused by drinking contaminated water. About 225 people are suffering from kidney diseases, it said.

According to the media report, about one and an half year back, the Central Government had issued data relating to areas affected with arsenic and fluoride. At that time, the maximum affected areas were found in Rajasthan. Apart from Chhattisgarh, Bihar, West Bengal, Assam, Jharkhand, Punjab, Odisha, Karnataka and Telangana are also suffering from the same health hazard.

*Original article online at http://www.uniindia.com/nhrc-issues-notices-to-c-garh-govt-ministry-of-drinking-water-over-high-levels-of-fluoride/india/news/1139592.html

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Wednesday, 14 February

22:24

Fluoride debate resurrected by Sarnia Mayor Mike Bradley "IndyWatch Feed Health"

Sarnia Mayor Mike Bradley wants residents to have a vote on fluoride. He is recommending the city have a plebiscite about the controversial compound included on the next civic-election ballot.

Bradley said whether or not to fluoridate drinking water is a perennial issue in many Canadian cities.

Ive been on 10 different councils and every council term this issue comes up, he said.

Bradley said the results would be non-binding, but would let council know how the public felt about the issue.

In 2013, Sarnia city council voted 5-4 in favour of getting rid of fluoride. The decision changed nothing, however. Sarnia is one of six municipalities to get their water from the Lambton Area Water Supply System (LAWSS). Fluoride is added to the water at one central water treatment plant, and the water is then pumped to the different municipalities. Unless four of those six municipalities vote to get ride of the controversial compound, the water continues to be fluoridated.

Susan MacFarlane is general manager at LAWSS.

Weve had many votes over the years, said Susan MacFarlane, general manager at LAWSS, adding that theyve always had fluoride at the plant.

Bradley said the last vote taken on fluoride at LAWSS ended in a 3-3 vote.

Fluoride since 1971

Bradley said there hasnt been a referendum on fluoride in Sarnia since 1971, when it was decided to add it to the water.

Sarnia back in the 50s and 60s, kids here had terrible teeth and it was the dentists who pushed for fluoride being in the water, he said. Things have changed.

Bradley said he supports keeping fluoride in the water.

However, I also respect that the next council, when it does come back up, should have the right to know what the public speaks, he said, adding the that a bonus of having a ballot question is that it can increase voter turnout.

Weve had ballot questions in the past that have brought people out to vote specifically on that issue, and then hopefully they vote for mayor and council, he said.

21:18

Fluoride coming to drinking water at Naval Base Kitsap-Bangor "IndyWatch Feed Health"

The Navy is in the process of installing a water fluoridation system at Bangor to comply with a Department of Defense directive issued in 2013.

Those who turn on the tap for a drink at Naval Base Kitsap-Bangor in 2020 will get water that includes fluoride.

The Navy is in the process of installing a water fluoridation system at Bangor to comply with a Department of Defense directive issued in 2013, said base spokesman Jake Chappelle.

That year, the Office of the Undersecretary of Defense mandated that all military installations serving more than 3,300 personnel through their own water treatment facilities install the systems.

Bangor has 9,000 military and civilian personnel on base.

Then-Assistant Secretary of Defense for Health Affairs, Dr. Jonathon Woodson, advocated for the institution of such a policy, since providing optimally fluoridated water at DoD installations helps to improve and sustain the military readiness and health of military personnel, Chappelle said.

Current research and scientific evidence shows that providing optimally fluoridated water directly reduces the risk of dental decay, he said.

The federal Department of Health and Human Services recommends a level of 0.7 milligrams of fluoride as the optimal level in drinking water.

It will cost a little more than $447,000 to install the system. It will not be operational until 2020 because of the required training and hiring of system operators, Chappelle said.

No other Naval Base Kitsap installations currently have or are planned to receive the fluoridation systems, because they do not meet the Department of Defenses base-size requirement of serving 3,300 personnel with a water system.

Joint Base Lewis-McChord has fluoridated water systems.

Kitsap County has one of the highest numbers of fluoridated public water systems in the state, according to the state Department of Health. Water systems with fluoride include the cities of Bainbridge Island, Poulsbo and Port Orchard; Bainbridges Rockaway Beach Water; the Manchester Water District; and Port Orchards West Sound Utility District #1.

Residents of the city of Bremerton voted down a proposal to fluoridate the water in 1999.

*Original article online at http://www.kitsapsun.com/story/news/local/2018/02/14/fluoridation-coming-bangors-drinking-water/329855002/

...

Sunday, 11 February

02:04

How to Cut Your Water Bill in Half With Ollas "IndyWatch Feed Health"

Source: How to Cut Your Water Bill in Half With Ollas For more content like this visit REALfarmacy.com.

An Olla (pronounced oh-yah) is a handmade terracotta clay pot used by ancient cultures as a simple form of drip irrigation. Today it can be utilized for containers, raised beds, and ground applications alike. Sustainability enthusiasts have found attaching ollas to a rainwater collection system is very functional and effective. Research has found that they were []

Source: How to Cut Your Water Bill in Half With Ollas Learn more at REALfarmacy.com - Healthy News and Information.

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